41results about How to "Increase local concentration" patented technology

The invention discloses a leaching reaction kettle. The leaching reaction kettle comprises a reaction tank, a stirring assembly, a guide cylinder and an aeration pipe. The stirring assembly comprises a stirring part; the flow guide cylinder is arranged in the reaction tank, the flow guide cylinder divides the reaction tank into an inner side space and an outer side space, the flow guide cylinder is arranged around the stirring part and defines the inner side space, the stirring part is used for driving fluid in the inner side space to flow upwards, the flow guide cylinder is provided with an upper opening and a lower opening, and the upper opening is communicated with the lower opening. The inner side space and the outer side space are communicated through the upper opening and the lower opening; and the aeration pipe is used for introducing reaction gas into the lower part of the inner side space. The leaching reaction kettle can improve the leaching efficiency of materials and the utilization rate of reaction gas.

The invention discloses an artificial antigen presenting cell for efficiently preparing a chimeric antigen receptor T cell (CAR-T) and a construction method of the artificial antigen presenting cell, and belongs to the field of cell therapy and immunotherapy, the artificial antigen presenting cell takes a K562 cell as a platform, an anti-CD3 single-chain antibody, an anti-CD28 single-chain antibody, a membrane binding type cell factor and a costimulatory ligand are stably expressed through genetic engineering, and the chimeric antigen receptor T cell (CAR-T) is obtained. And the local concentration of the anti-CD3 antibody is improved by adopting an HOTag3 tool. The multi-signal integration strategy obviously enhances the activation and proliferation capacity of the T cells, promotes the CAR-T cells to maintain a low differentiation state, and endows the CAR-T cells with excellent anti-tumor activity and durability. The artificial antigen presenting cell provides a novel efficient tool for the field of cellular immunotherapy, and is expected to promote development of more efficient and safer CAR-T therapy to cope with challenges of various malignant tumors.

The invention provides a curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound as well as a preparation method and application thereof, and belongs to the field of medicines. The curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound is a compound prepared by taking curcumin, antibacterial peptide and tetrahedral framework nucleic acid as raw materials. Cell experiments show that the curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound can significantly improve the osteogenesis activity of periodontal ligament stem cells, recover the mineralization activity, reduce the oxidative stress level and inhibit the occurrence and development of inflammatory reactions. Animal experiments show that the curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound can significantly reduce loss of alveolar bones of periodontitis rats, inhibit the inflammatory state, promote osteogenic activity, recover the structure and quality of the alveolar bones and effectively treat periodontitis. The curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound provided by the invention has a wide application prospect in preparation of drugs for preventing and / or treating periodontitis.

The invention relates to a long-acting whitening and blackening-free composition containing glabridin and pterostilbene and application of the long-acting whitening and blackening-free composition, and belongs to the technical field of whitening cosmetics. The composition provided by the invention is prepared from the following components in parts by weight: 0.005 to 0.1 part of glabridin, 0.03 to 0.4 part of pterostilbene, 0.02 to 0.2 part of herba centellae extract, 0.0005 to 0.005 part of palmitoyl tripeptide-8 and 0.01 to 0.1 part of sodium hyaluronate. The application of the composition provided by the invention comprises the following steps: (1) preparing a phase A containing an inclusion empty shell; (2) dissolving glabridin, pterostilbene and palmitoyl tripeptide-8 to obtain a phase B; dissolving the centella asiatica extract and sodium hyaluronate to obtain a phase C; (3) adding the phase A and the phase C into the phase B, and uniformly mixing to obtain an inclusion phase; and (4) compounding the inclusion phase with a cosmetic base material. The composition provided by the invention integrates melanin regulation and control and skin barrier repair, enhances the whitening effect through targeted transmission and inclusion slow release, prolongs the blackening time, and has a very good application prospect in the whitening cosmetic industry.

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.

The invention relates to an andrographolide nanocrystal suspension, preparation thereof and application of the andrographolide nanocrystal suspension in preparation of an andrographolide nanocrystal dry powder inhaler with an anti-inflammatory effect. The andrographolide nanocrystal suspension contains a) an active component andrographolide nanocrystal; b) a stabilizer; wherein the content of andrographolide in the suspension is 0.1%-30%, and the content of the stabilizer is 0.1%-5%. According to the andrographolide nanocrystal dry powder inhalant and the preparation method thereof, andrographolide nanocrystals are prepared into micro powder by adopting a spray drying technology, and auxiliary material addition and a preparation process are optimized, so that the andrographolide nanocrystal dry powder inhalant with good inhalation performance is successfully prepared and directly acts on the respiratory tract through a pulmonary drug delivery system, the administration dosage is reduced, and the inhalation effect is improved. Higher lung local concentration is obtained, the first-pass effect of the liver is avoided, meanwhile, the efflux effect of p-glycoprotein is avoided as much as possible, and then the bioavailability is improved; the technical problem that the bioavailability is poor due to the fact that andrographolide is excreted through p-glycoprotein and rapidly absorbed and metabolized in the intestinal tract is solved.

The invention relates to the technical field of medical instruments, in particular to an intravascular stent. According to the specific technical scheme, the intravascular stent comprises a stent skeleton, after the stent skeleton is subjected to surface treatment, at least one layer of film covering section is arranged outside the stent skeleton, and medicine release sections are arranged at the two ends of the stent skeleton. The problems that in the drug release process of an existing stent, the drug release rate is uneven, biocompatibility is poor, and thrombus and restenosis are caused by a metal stent are solved.

The invention discloses a targeted nano delivery system for pulmonary fibrosis treatment and a preparation method of the targeted nano delivery system. The lung targeting nano delivery system comprises a carrier and a substance A, the carrier is a nano-particle formed by TM2 or a nano-particle formed by TM2 and DOPE (Dioctyl Olefin Polyethylene); the structural formula of the TM2 is shown as a formula I; the substance A is selected from at least one of the following substances: 1) a substance for inhibiting the expression of a TTC3 gene, 2) a substance for knocking out the TTC3 gene, and 3) a substance for inhibiting the activity or content of the TTC3. The targeted nano-delivery system can reach the deep part of lung tissue through oral trachea administration to realize the effect of safely and effectively delivering drugs.

The invention discloses a conductive aeration membrane cathode electro-Fenton sewage treatment device and method based on iron circulation and application. The invention belongs to the technical field of electro-Fenton sewage treatment. The invention aims to solve the technical problems of low O2 utilization rate, poor ferrous cyclic regeneration performance and poor selective degradation effect in the current electro-Fenton complex water treatment technology. The nano confinement conductive gas diffusion film is composed of a base film and an active layer, wherein the active layer is composed of fractured CNT and internal BN. An aeration membrane assembly is arranged in the reaction chamber of the device, and the surface, adjacent to the anode, of the assembly is composed of the gas diffusion membrane. By means of the design, oxygen directly makes contact with the catalyst layer through the gas diffusion film and is consumed immediately, and mass transfer resistance generated by water when the oxygen is diffused to the catalyst layer is avoided while 100% utilization of the oxygen is achieved. The nanometer confinement conductive aeration membrane cathode is high in ferrous cyclic regeneration capacity and has a good treatment effect in actual nanofiltration concentrated water, and selective degradation of ECs is achieved.

The invention belongs to the technical field of preparation of four-membered ring derivatives, and particularly relates to a four-membered ring derivative and a preparation method thereof. The preparation method of the four-membered ring derivative comprises the following steps: (1) adding two substrates, a photocatalyst and a solvent into a quartz tube, and stirring; (2) putting the quartz tube under ultraviolet light to carry out photocatalytic reaction; and (3) evaporating the reaction liquid to dryness, and separating through column chromatography to obtain the target compound. According to the invention, cis-olefin is used as an initial raw material to carry out cycloaddition reaction, so that the method has high regioselectivity and diastereoselectivity; the homodimerization reaction of olefin is effectively inhibited through reversible cis-trans isomerization reaction, so that the four-membered ring derivative is efficiently obtained. The four-membered ring derivative prepared by the invention plays a basic role in drug design, and provides a solid structural support for medical molecules.

The invention belongs to the technical field of environmental pollution treatment and water treatment, and particularly relates to a method for degrading organic pollutants by coupling a nano confinement catalytic membrane with micro-nano bubbles. The method comprises the following steps: adding micro-nano bubble water and an oxidizing agent into an organic pollutant solution, regulating the pH value to prepare a pretreatment mixed solution, and enabling the pretreatment mixed solution to pass through a nano confinement catalytic membrane to catalytically degrade the organic pollutants, the nano confinement catalytic membrane comprises an organic polymer membrane substrate and a catalyst loaded in the organic polymer membrane substrate; the loading capacity of the catalyst is 5 to 30 mg. The problems that antibiotics are difficult to degrade, pollutant concentration is easily caused by membrane separation, a catalyst is difficult to recycle, and membrane flux and catalytic activity are difficult to balance are solved, the method has the advantages of being high in degradation efficiency, stable in membrane flux, easy to recycle the catalyst and wide in application range, and wastewater containing organic pollutants can be efficiently treated.

The present invention provides a detection system for early tumor screening and its application in the preparation of tumor antigen detection products, belonging to the technical field of early tumor screening. The present invention provides the application of a micro-pot array chip combined with micron-sized magnetic beads in the preparation of an ELISA detection product for early tumor screening. The present invention uses micron-sized hydrophilic micro-pots as reaction carriers for ELISA detection, and uses micron-sized magnetic beads to encapsulate capture antibodies used to capture tumor antigens. By limiting the fluorescent groups produced by a single enzyme-catalyzed substrate to an extremely small volume, a high local concentration of fluorescent product molecules is ensured to generate a sufficiently strong optical signal to be detected, thereby realizing the detection of extremely low concentrations of tumor antigens. At the same time, the present invention can reduce the amount of sample collection, such as the amount of blood collection, and the amount of serum required for detection can be achieved by drawing blood from the fingertips, greatly reducing the degree of trauma to the patient.

The invention provides a composition for improving the thrombolysis capacity of a human body, a preparation method and application thereof.The composition is prepared from, by weight, 5-30 parts of earthworm protein, 30-120 parts of hippophae rhamnoides total flavone extract, 50-150 parts of silymarin extract, 20-80 parts of maltodextrin and 15-60 parts of pregelatinized starch, the earthworm protein activates a fibrinolytic system to promote t-PA to release and dissolve thrombus, and the hippophae rhamnoides total flavone extract activates the fibrinolytic system to promote t-PA to release and dissolve thrombus; the hippophae rhamnoides total flavonoids regulate the ratio of PGI2 / TXA2 to inhibit platelet aggregation and reduce blood fat, the silymarin protects the liver function to enhance LDL-C metabolism clearing, the hippophae rhamnoides total flavonoids, the silymarin and the hippophae rhamnoides total flavonoids cooperate to form a thrombolysis-antithrombosis-metabolism regulation three-dimensional intervention system, a whole-course low-temperature mixing direct tabletting process is adopted

The utility model belongs to the technical field of waste treatment, and discloses a demisting device for an absorption tower, the demisting device comprises a demisting assembly I and a demisting assembly II, the demisting assembly I is coaxially mounted in an air inducing pipeline, a backflow cavity I is positioned between the side wall of a reaction cavity and the inner side wall of the absorption tower, the top end of the backflow cavity I is open, and the air inducing pipeline is communicated with the backflow cavity I; a second backflow cavity is formed between the outer side wall of the air inducing pipeline and the outer cavity wall of the top end of the reaction cavity, the second backflow cavity is communicated with the air inducing pipeline, a hydrophilic semi-permeable membrane is arranged at the communication position, and the side end of the second backflow cavity is communicated with the first backflow cavity; the bottom of the exhaust cavity communicates with an air inlet pipeline, and the lower end of the air inlet pipeline communicates with the air inducing pipeline and the first backflow cavity. The second demisting assembly is installed in the air inlet pipeline, and an exhaust port communicating with the outside is formed in the top of the exhaust cavity. According to the demisting device, the flowing path of liquid is changed, the situation that the liquid and gas are repeatedly mixed to generate mist again can be reduced, secondary demisting is conducted through the second demisting assembly, and the demisting effect is improved.

This invention provides a catalyst for the hydroxylation reaction of p-hydroxybenzonitrile and its preparation method, belonging to the field of catalyst technology. The method includes the following steps: Step S1, preparing a pure silica support using pure silica molecular sieves, then loading and activating it with copper to obtain an activated copper-supported support; Step S2, further preparing a copper-based functionalized support; Step S3, pre-assembling p-hydroxybenzonitrile, polymerizable β-cyclodextrin functional monomers, N-vinylimidazolium, and acrylamide, then adding the copper-based functionalized support, ethylene glycol dimethacrylate, and AIBN for polymerization to obtain the catalyst for the hydroxylation reaction of p-hydroxybenzonitrile. This invention is beneficial for improving the dispersion stability of copper species and the substrate enrichment ability, as well as improving the selectivity of the hydroxylation reaction of p-hydroxybenzonitrile and the catalyst recycling performance.

The invention discloses an electrically-mediated iron-carbon composite filler constructed wetland system for removing sulfonamide antibiotics and resistance genes as well as a construction method and a use method of the electrically-mediated iron-carbon composite filler constructed wetland system, and belongs to the technical field of water treatment. Coconut shell biochar is modified by hydrochloric acid and then compounded with pyrite to form a composite filler with targeted adsorption, efficient electron conduction and multi-mechanism catalysis functions, and the composite filler is used as a core matrix to construct a vertical flow constructed wetland system applying an external direct-current electric field. The system realizes efficient, synchronous and stable removal of sulfonamide antibiotics and resistance genes thereof in a water body by virtue of directional enrichment of the filler on pollutants, an electron transfer network formed by a composite material and an electrochemical-microorganism synergistic effect jointly excited by a direct-current electric field and the filler.

The application discloses a kind of phosphogypsum leachate processing method and device, it is related to sewage treatment technical field, comprising: reaction tank and fixedly connected in the inside of the reaction tank support seat, the inside of the support seat is rotatably connected with stirring rod, the top of the support seat is equipped with first drive motor, and the output of the first drive motor is fixedly connected with the stirring rod;Suction aid, located in the inside of the reaction tank, for auxiliary with the sewage at the bottom of the reaction tank is pumped to top and mixed reagent discharge.The application is by being provided with suction aid, so that sewage forms the circulating flow of "bottom-top", this process can avoid the local concentration of bottom sewage due to gravity deposition (or pollutant enrichment) too high, while let top not fully mixed sewage and bottom circulating sewage again contact, so that the sewage concentration in the whole reaction tank is more uniform.

The invention discloses a composition for miRNA detection based on an infinite zipper hybridization chain reaction (ZHCR), a fluorescent biosensor as well as a construction method and application of the fluorescent biosensor. According to the invention, HCR and PER are combined, a single-stranded DNA product generated by PER is used as a track for fixing hairpins, and ZH1 and ZH2 are generated by respectively reacting and combining with legged hairpins H1-l and H2-l of HCR; a target recognition unit is further designed according to the miRNA sequence, after the target miRNA is combined with the target recognition unit, a closed chain falls off, an initiation sequence is exposed, ZH2 and ZH1 are initiated to generate ZHCR, and a fluorescence signal is generated. The preparation of the reaction substrate and the sequence design are simple, and the free hairpin can be positioned on a long track, so that the reaction rate is improved, isothermal, rapid and sensitive amplification of miRNA in vitro is realized, and a new platform with a prospect can be provided for efficient imaging and in-vitro detection of miRNA in living cells.

The invention discloses a multienzyme synergistic catalysis carbon sequestration method, and belongs to the technical field of bioengineering. Virus-like nanoparticles P22 composed of capsid protein (CP) and scaffold protein (SP) are used as a platform for constructing a carboxyl body simulant, carbonic anhydrase (CA) and ribulose-1, 5-diphosphate carboxylase / oxygenase (RuBisCO) are connected to the scaffold protein in a gene fusion mode, the non-covalent self-assembly characteristic of CP and SP is utilized, and the carboxyl body simulant is constructed through the non-covalent self-assembly characteristic of CP and SP. The artificial carboxyl body based on the virus-like particles is constructed by adding the virus-like particles into the carrier to realize intracellular and extracellular recruitment of the cargo protein, the catalytic efficiency of RuBisCO is further improved through multi-enzyme synergistic catalysis, and the artificial carboxyl body can be used for enhancing the CO2 immobilization capability of cells. According to the invention, a simple carboxyl body simulant is constructed from bottom to top, and early evolution benefits of multi-enzyme concerted catalysis realized by co-localization of CA and RuBisCO are deeply understood, so that a new solution idea is provided for better understanding the carbon sequestration function of a carboxyl body.

The utility model discloses an organic matter purifying agent raw material pretreatment device, and the device includes the stirring tank with the cone bottom and is arranged in the agitator of stirring tank, wherein, the agitator includes stirring shaft and sets up the intermediate stirring structure, the outer periphery stirring structure and the bottom stirring structure on stirring shaft, the outer periphery stirring structure includes the extension arm fixed in stirring shaft both sides, the extension arm end is provided with the stirring column and is provided with the stirring arc plate between stirring column, the stirring arc plate shape is semicircular arc plate, and one end is fixed in the upper end of one stirring column, and the other end is fixed in the lower end of another stirring column. The device is through multilayer stirring and optimization feeding design, improves the utilization of medicine, reduces the operating cost, prevents the equipment from blocking, is applicable to industrial wastewater and domestic sewage treatment, and significantly improves the mixing uniformity and process efficiency.

The invention relates to the technical field of traditional Chinese medicine composition production, in particular to a traditional Chinese medicine composition for rapidly relieving renal colic and a preparation method thereof. Comprising the following components in parts by weight: 10-30 parts of lysimachia christinae hance nanoparticles, 6-16 parts of rhizoma corydalis, 8-18 parts of radix paeoniae alba, 5-15 parts of endothelium corneum gigeriae galli, 8-18 parts of rhizoma alismatis, 5-10 parts of lygodium japonicum, 2-8 parts of beta-cyclodextrin, 0.5-1.5 parts of a poloxamer surfactant and 2-8 parts of sodium carboxymethyl cellulose. According to the invention, flavonoid glycoside in lysimachia christinae hance is extracted, glycosylated and emulsified into nano-particles, so that the absorption and utilization rate of flavonoid glycoside in a body are improved, and the drug effect of flavonoid glycoside is improved. Beta-cyclodextrin and sodium carboxymethyl cellulose are adopted as carriers, the release speed of the traditional Chinese medicine composition can be regulated and controlled, the release speed of components is slowed down to a certain degree, and the medicine can continuously play a role in the body.

The invention discloses a jacket liner type reactor based on supergravity, a reaction method and application, the reactor comprises a reactor body, and is characterized in that a material circulation assembly is arranged in the reactor body, a supergravity generation unit is arranged outside the reactor body, the supergravity generation unit penetrates through the reactor body, and the material circulation assembly is connected with the supergravity generation unit. The output end of the supergravity generating unit extends into the material circulating assembly, a rotor assembly matched with the material circulating assembly is arranged at the output end of the supergravity generating unit, and the rotor assembly comprises a material suction cavity in the center and a filler cavity arranged around the material suction cavity; the material circulation assembly is provided with a circulation material input port and a circulation material output port, materials entering the material suction cavity enter the material circulation assembly after passing through the filling cavity under the action of centrifugal force, enter the reactor body through the circulation material output port, and then enter the material suction cavity through the circulation material input port for circulation. The reactor has the advantages of good mass and heat transfer effect and high reaction efficiency.

The invention belongs to the technical field of anti-freezing hydrogel, and particularly relates to anti-freezing hydrogel and application of the anti-freezing hydrogel in preparation of a rapid trauma hemostasis product, and the anti-freezing hydrogel is prepared through the following steps: 1) preparing a nanocellulose solution; 2) adding a catalyst, a cross-linking agent and dopamine into the nanocellulose solution, and reacting to obtain a first mixed solution; 3) cooling the first mixed solution, dialyzing and drying to obtain an intermediate solid product; 4) mixing the intermediate solid product with water to obtain a second mixed solution, then adding glycerol and polyvinyl alcohol, and after the intermediate solid product is completely dissolved, adding a borax solution to obtain a third mixed solution; and 5) adding a coagulation promoting drug into the third mixed solution to obtain a fourth mixed solution, reacting at 90-92 DEG C for 0.5-1 hour, cooling to room temperature, and molding to obtain the anti-freezing hydrogel. The material has excellent tissue adhesion, freezing resistance and rapid hemostatic effect.

This invention relates to the field of pharmaceutical intermediate synthesis technology, and discloses a method for the continuous preparation of 4-AA using a highly stable supported ruthenium catalyst. The method involves preparing a substrate solution by combining a 4-AA precursor cyclizer, acetate, and an acetonitrile-acetic acid mixed solvent. The resulting liquid peracetic acid is directly used as the oxidant solution. After pre-cooling, the solution is continuously fed into a fixed-bed reactor packed with the supported ruthenium catalyst for oxidative acetylation. The oxidant is added in stages along the material flow direction. The reaction solution undergoes reducing quenching, extraction, washing, concentration, and crystallization to obtain 4-AA. This invention reduces ruthenium component migration and loss, shortens the residence time of liquid peracetic acid before the reaction, reduces its decomposition risk, improves reaction heat transfer and oxidant concentration distribution, and enhances continuous operation stability and reaction safety.

The invention relates to the technical field of lead ion detection, in particular to a lead ion detection colorimetric sensor and application thereof.The lead ion detection colorimetric sensor comprises hemin, a substrate chain S, an enzyme chain Dz and a DNA chain DS with three palindromic structures; in the absence of Pb < 2 + >, the substrate chain S and the enzyme chain Dz are hybridized to form a stable S-Dz double chain; when Pb is introduced, an enzyme chain Dz can be activated, and lead ions are specifically recognized and used as cofactors. Through an annealing process, the DNA chain DS is subjected to intramolecular hybridization and self-folding so as to be self-assembled into a definitely-defined and monodisperse DNA nanosphere; the substrate chain S and the enzyme chain Dz can be hybridized to form a stable S-Dz double chain; a plurality of S-Dz are concentrated on a limited nanosphere surface (DS-S-Dz), the catalysis direction is optimized, side reactions are reduced, and a signal DNAzyme can be quickly formed, so that a color signal is remarkably amplified, and lead ion detection with higher sensitivity, lower background and higher stability is realized.

This invention discloses the application of a fullerol-supported palladium nanowire composite material in the electrocatalytic dechlorination of 2,4-dichlorophenol. The fullerol-supported palladium nanowire composite material is loaded onto a conductive substrate to form a working electrode. This working electrode is used as the cathode of an electrochemical reactor to perform an electrocatalytic dechlorination reaction on wastewater containing 2,4-dichlorophenol. This invention, by modifying fullerol-modified palladium nanowires, yields a highly promising electrocatalyst for the dechlorination of 2,4-dichlorophenol, which enhances the adsorption and activation capacity for 2,4-dichlorophenol, thereby improving catalytic selectivity and reaction rate.

The present invention discloses an in vivo capture system for cells to be tested and a working method thereof, the system comprising an immune micro-nano bubble supply device, an acoustic resonance immune guide body in vivo delivery device, an ultrasonic emitting device and an acoustic resonance immune guide body; the immune micro-nano bubble supply device provides immune micro-nano bubbles coupled with antibodies, the immune micro-nano bubbles are injected into the body to specifically bind to the cells to be tested to form a bubble complex; the guide body in vivo delivery device delivers the acoustic resonance immune guide body to the in vivo area to be tested; the ultrasonic emitting device emits ultrasonic waves to stimulate the acoustic resonance immune guide body in the body to generate a local strong field; the surface coupling of the acoustic resonance immune guide body can identify antibodies of the cells to be tested, and when being delivered to the body, it specifically binds to the cells to be tested to capture the cells to be tested, and uses the local strong field to adsorb the bubble complex to capture the cells to be tested. The present invention can increase the number of captured trace cells to be tested in the body and improve the accuracy of subsequent detection.

The invention belongs to the technical field of the crossing field of nano biological materials and intelligent drug delivery systems, and discloses a dual-power liposome nano motor and a preparation method and application thereof, the dual-power liposome nano motor comprises liposome, metal nanoparticles and DNAzyme, the metal nanoparticles have catalase-like activity, and the DNAzyme has the activity similar to catalase. The metal nanoparticles and the DNAzyme are entrapped by the lipidosome. The dual-power liposome nano-motor provided by the invention is applied to tumor treatment, gene therapy regulates gene expression to inhibit tumor growth, chemodynamic therapy can destroy tumor cells through chemical reaction of drugs, and photodynamic therapy energy is transmitted to surrounding oxygen, so that the tumor cells are prevented from being damaged. According to the present invention, the three treatment modes are combined to form the complementary advantages, such that the treatment effectiveness is improved, and the occurrence of the adverse reaction is reduced; the singlet oxygen with the strong activity is subjected to the oxidation reaction with the nearby biological macromolecules, and the generated cytotoxicity can kill the cancerous cells; and the three treatment modes are combined together so as to form the complementary advantages, such that the treatment effectiveness is improved, and the occurrence of the adverse reaction is reduced.

This invention discloses a magnetic drug-loaded liposome for nerve repair and its preparation method. The magnetic drug-loaded liposome prepared by this method contains magnetic nanoparticles and a drug. The mass fractions of egg yolk lecithin, cholesterol, DSPE-MPEG 2000, BFe3O4, and the drug in 1 mL of the liposome solution are 0.5%–1.5%, 0.15%–0.35%, 0.1%–0.35%, 0.1%–0.2%, and 0.1%–0.2%, respectively. The drug is one or more of the natural antioxidants such as piracetamine, resveratrol, or tea polyphenols. The multifunctional magnetic drug-loaded liposome provided by this invention, combined with magnetic field loading, can be used for the repair of damaged nerve cells.

This invention relates to the field of microneedle drug delivery technology, specifically to a soluble microneedle and its preparation method. The soluble microneedle includes a needle hub and at least one needle body located on the needle hub. The needle body includes a first intermediate segment, a second intermediate segment, and a needle tip. The first intermediate segment is located on the needle hub, and the second intermediate segment is located between the first intermediate segment and the needle tip. The density of the second intermediate segment is greater than that of the first intermediate segment. The needle tip contains a drug active ingredient. The soluble microneedle provided by this invention has the drug active ingredient concentrated in the needle tip. Furthermore, the second intermediate segment, which is in contact with the needle tip, is a high-density segment, which can prevent the drug active ingredient in the needle tip from diffusing into the second intermediate segment and the first intermediate segment. Therefore, the soluble microneedle of this invention can significantly increase the local drug delivery concentration, achieving precise, targeted, and quantitative drug delivery.