Preparation method of conductive double-layer hydrogel for integral regeneration of bone and cartilage by electrical stimulation

A hydrogel and osteochondral technology, applied in tissue regeneration, prosthetics, medical science, etc., can solve the problems of poor host tissue integration, poor mechanical properties, poor repair effect, etc., to improve mechanical properties, good Tissue adhesion, improve the binding effect

Inactive Publication Date: 2019-08-16
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrogel is poorly integrated with the host tissue and cannot be repaired by gradient
However, the traditional osteochondral integrated repair hydrogel is mainly composed of natural polymers, which has the disadvantage of poor mechanical properties and cannot withstand the complex stress of the osteochondral injury site.
resulting in a poor repair

Method used

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  • Preparation method of conductive double-layer hydrogel for integral regeneration of bone and cartilage by electrical stimulation
  • Preparation method of conductive double-layer hydrogel for integral regeneration of bone and cartilage by electrical stimulation
  • Preparation method of conductive double-layer hydrogel for integral regeneration of bone and cartilage by electrical stimulation

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preparation example Construction

[0028] A method for preparing a conductive double-layer hydrogel for electrical stimulation of osteochondral integral regeneration, comprising the following steps:

[0029] Step 1: Disperse conductive particles and dopamine monomers in water to form a suspension. The total concentration of dopamine and conductive particles in the suspension is 1-20 mg / L, and the mass ratio of dopamine to conductive particles is 0.25-4:1. Adjust the pH of the solution to 8-9 with calcium hydroxide at a concentration of 30-60 mg / L, and stir at 50-90°C for 2-10 hours. After the reaction is completed, calcium hydroxide solution A containing dopamine-modified conductive particles is obtained .

[0030] Step 2: Dissolve polyvinyl alcohol in deionized water to prepare polyvinyl alcohol solution B with a concentration of 200-500 mg / L; after complete dissolution, add calcium hydroxide solution A containing conductive particles modified by dopamine, wherein, The volume ratio of solution A and solution ...

Embodiment 1

[0036] A method for preparing a conductive double-layer hydrogel for electrical stimulation of osteochondral integral regeneration, comprising the following steps:

[0037] Step 1: disperse graphene and dopamine monomers in water to form a suspension, the total concentration of dopamine and graphene in the suspension is 1 mg / L, and the mass ratio of dopamine and graphene is 0.25-4:1. The pH of the solution was adjusted to 8 with calcium hydroxide at a concentration of 30-60 mg / L, stirred at 50°C for 2 hours, and a calcium hydroxide solution A containing dopamine-modified graphene was obtained after the reaction was completed.

[0038] Step 2: Dissolve polyvinyl alcohol in deionized water to prepare polyvinyl alcohol solution B with a concentration of 200 mg / L; after completely dissolving, add calcium hydroxide solution A containing dopamine-modified graphene, wherein solution A The volume ratio of solution B and solution B is 1:1, and mixed solution C is obtained after uniform...

Embodiment 2

[0045]A method for preparing a conductive double-layer hydrogel for electrical stimulation of osteochondral integral regeneration, comprising the following steps:

[0046] Step 1: Disperse single-walled carbon nanotubes and dopamine monomers in water to form a suspension. The total concentration of dopamine and single-walled carbon nanotubes in the suspension is 5 mg / L, and the mass ratio of dopamine to single-walled carbon nanotubes is 1:1. The pH of the solution was adjusted to 8.5 with calcium hydroxide at a concentration of 40 mg / L, and stirred at 60° C. for 3 hours. After the reaction was completed, a calcium hydroxide solution A containing dopamine-modified single-walled carbon nanotubes was obtained.

[0047] Step 2: Dissolve polyvinyl alcohol in deionized water to prepare polyvinyl alcohol solution B with a concentration of 300 mg / L; after completely dissolving, add calcium hydroxide solution A containing dopamine-modified single-walled carbon nanotubes, wherein , the...

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Abstract

The invention discloses a preparation method of a conductive double-layer hydrogel for integral regeneration of bone and cartilage by electrical stimulation. The preparation method comprises the following steps: step 1, dispersing conductive particles and dopamine monomers in water to form a suspension liquid, adding a calcium hydroxide solution to adjust the suspension liquid to be alkalescent, and carrying out a full reaction to generate a calcium hydroxide solution A containing dopamine-modified conductive particles; step 2, preparing a polyvinyl alcohol solution B, adding the polyvinyl alcohol solution B into the solution A to obtain a mixed solution C; step 3, dropwise adding phosphoric acid into the mixed solution C for a reaction, then carrying out forming, and carrying out freeze-thawing circulation to obtain a lower-layer hydrogel; step 4, dissolving polyvinyl alcohol, natural macromolecules and dopamine in deionized water to form a mixed solution, and carrying out a completereaction under a weak alkaline condition to obtain a solution D; and step 5, placing the solution D on the lower-layer hydrogel, and carrying out freeze-thawing circulation to obtain the required double-layer hydrogel. The double-layer hydrogel disclosed by the invention is good in tissue adhesion and electrical stimulation response capability.

Description

technical field [0001] The invention relates to the technical field of biomaterial preparation, in particular to a method for preparing a conductive double-layer hydrogel for electrical stimulation of osteochondral integral regeneration. Background technique [0002] Bone and cartilage-related diseases are the most common diseases in humans, and they affect millions of people worldwide. One of the main difficulties in osteochondral integrated repair is that three aspects must be considered: bone, cartilage, and the bone-cartilage interface. Among them, the osteochondral (bone-cartilage) interface is the fixation point of hyaline cartilage and subchondral bone and thus plays a key connection role. In addition, it provides the mechanical structure that supports energy transfer from joints to bones for biomechanical movement. Due to the poor regeneration ability of hyaline cartilage, damaged osteochondral tissue is difficult to treat. Recovery from injury is also difficult d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L29/04C08L89/00C08L5/08C08L79/04C08K9/04C08K3/04C08K3/32A61L27/40A61L27/16A61L27/12A61L27/22A61L27/08A61L27/20A61L27/18A61L27/50A61L27/52A61L27/58
CPCA61L27/08A61L27/12A61L27/16A61L27/18A61L27/20A61L27/222A61L27/227A61L27/50A61L27/52A61L27/58A61L2430/02A61L2430/06C08J3/075C08J2329/04C08J2405/08C08J2479/04C08J2489/00C08K9/04C08K2003/325C08K2201/001C08K2201/011C08K3/041C08K3/042C08L29/04C08L89/00C08L5/08C08L79/04
Inventor 鲁雄罗嘉庆谢超鸣
Owner SOUTHWEST JIAOTONG UNIV
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