A diblock fusion protein with dual functions of adhesion and antifreeze, its synthesis method and application

A fusion protein and synthesis method technology, applied in the biological field, can solve the problems of cumbersome operation, cumbersome preparation method of superhydrophobic material, protein inactivation, etc., and achieves the effects of low equipment requirements, easy later production expansion, and low cost.
CN110776569BActive Publication Date: 2022-03-15TIANJIN UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2022-03-15

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to a diblock fusion protein with dual functions of adhesion-antifreeze and its synthesis method and application; a gene expression carrier containing antifreeze protein gene and adhesion protein gene is prepared by using genetic engineering as a technical means, And transform it into host cells, make it overexpress the protein by inducer, separate and purify to obtain the fusion protein. The amino acid sequence of the fusion protein is SEQ ID NO.3, and the nucleotide sequence is SEQ ID NO.4. Mussel adhesion protein and antifreeze protein were fused at the gene level to construct a two-block fusion protein with dual functions of adhesion and antifreeze; the fusion protein was expressed by Escherichia coli fermentation, and purified by affinity chromatography Albumin, using acetic acid to purify inclusion bodies, constructs a high-purity separation and purification method, and realizes the one-step synthesis of fusion proteins by microorganisms. This process is mild and efficient, low in cost, low in equipment requirements, and easy to expand production in the later stage.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The present invention belongs to the field of biotechnology, and more particularly to two-block fusion proteins and synthesis methods and applications having adhesive-antifreeze double function; including gene design of two-block fusion proteins having adhesion-antifreeze dual function, Construction of recombinant expression vectors containing a fusion protein gene fragment, fermentation expression, fusion protein separation, and its application in antifreeze surfaces in E. coli. Background technique

[0002] The antifreeze protein (AFP) is a class of protein compounds having improved biological antifreeze resistance, which can bind to small crystals, prevent ice crystallization and crystal growth. Typically, the larger the resection of the antifreeze protein is, the better the organism to low temperature. (The thermal hysteresis effect is a refrigeration of the solution in a non-agreed, and does not change its melting point, so that the ice point and melti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More