21 results about "Mytilus coruscus" patented technology

The invention relates to a mytilus coruscus byssus adhesion protein gene Mfp5 coding region SNP marker and application thereof, the mytilus coruscus byssus adhesion protein gene Mfp5 coding region SNP marker comprises a gene sequence numbered as an McMfp5 coding region, the nucleotide sequence of the gene sequence is shown as SEQ ID No.1, an SNP marker primer pair is designed based on the sequence, three SNP markers numbered as McMfp51, McMfp52 and McMfp53 are obtained through sequencing, and the primer sequences of the three SNP markers are shown as SEQ ID No.2 and SEQ ID No.3 in sequence. The primer designed aiming at the gene sequence of the McMfp5 coding region is used for SNP (Single Nucleotide Polymorphism) marker typing, is high in specificity, stable in amplification and high in polymorphism, can be used for genetic diversity analysis, germplasm identification and marker-assisted selection breeding of mytilus coruscus populations, and provides a powerful tool for genetic breeding of mytilus coruscus.

The invention discloses a preparation method of high-purity recombinant mussel mucoprotein, and belongs to the technical field of recombinant protein preparation, the idea of improving target protein expression by adopting fusion protein is adopted, and fusion expression is performed on partial peptide fragments of an amino acid sequence of mytilus coruscus Mfp-3 and an amino acid sequence of common mussel preCol-D to obtain the high-purity recombinant mussel mucoprotein. The recombinant mussel mucin which is high in purity, low in endotoxin content and good in adhesion performance is obtained by combining improvement of induction, tyrosinase modification and separation and purification processes, a new thought is provided for industrial production of the recombinant mussel mucin through successful preparation of the recombinant mussel mucin, raw material support is provided for multi-field application of the recombinant mussel mucin, and the method is suitable for industrial production of the recombinant mussel mucin. The customized sequence recombinant mussel mucoprotein is prepared according to application requirements, and the application of the mussel mucoprotein in different fields is promoted.

The application discloses a preparation method of high-purity recombinant mussel adhesive protein and belongs to the technical field of recombinant protein preparation. The amino acid sequence of Mfp-3 of Mytilus coruscus and the partial peptide segment of the amino acid sequence of preCol-D of Mytilus edulis are fused and expressed according to the idea of improving target protein expression by using fusion protein, and the improvement of the induction, tyrosinase modification and separation and purification process is combined to obtain the recombinant mussel adhesive protein with high purity, low endotoxin content and good adhesion performance. The successful preparation of the recombinant mussel adhesive protein provides a new idea for the industrial production of the recombinant mussel adhesive protein, provides raw material support for the multi-field application of the recombinant mussel adhesive protein, realizes the preparation of the customized sequence recombinant mussel adhesive protein according to application requirements, and promotes the application of the mussel adhesive protein in different fields.

The invention relates to the field of aquatic product processing equipment, and discloses a device for stripping mytilus coruscus bulls through water jet at low temperature, the device comprises an operation table, a first sliding rail and a mounting table are arranged on the operation table, a placing plate is slidably connected to the first sliding rail, and a fixing assembly is arranged on the placing plate; a water jet stripping mechanism and a waste water circulating mechanism are arranged in the operation table, an anti-freezing assembly is arranged on the water jet stripping mechanism, a displacement mechanism and cooling equipment are arranged on the mounting table, the mounting table is connected with a mounting block through the displacement mechanism, and an angle fine adjustment mechanism is arranged on the mounting block. The high-pressure water jet stripping technology is combined with the visual sensor to achieve efficient and accurate stripping, the stripping efficiency is improved, stable operation of equipment in a low-temperature environment is ensured through an anti-freezing pipe and dynamic heating, the problem that the stripping efficiency is low due to water flow freezing is solved, and water resources are saved and the operation cost is reduced through the wastewater circulation mechanism.

The utility model relates to the technical field of mytilus coruscus breeding, and discloses a mytilus coruscus ecological breeding floating bed which comprises a PVC surrounding pipe and a foam column, a PVC net plate is fixedly installed in the PVC surrounding pipe, a plurality of meshes are formed in the PVC net plate, and a remote control receiving box is fixedly connected to the side face of the PVC surrounding pipe. According to the ecological breeding floating bed for mytilus coruscus, connecting arms on the two sides are used for controlling damping rods and springs to stretch, positioning columns on the side faces of the connecting arms and the connecting rods move outwards, then foam columns are aligned to the positioning columns, the connecting arms are loosened and reset under the potential energy action of the damping rods and the springs, and then the positioning columns position the foam columns; the whole buoyancy can be enhanced by utilizing the foam column, the floating bed is controlled in a remote control mode in the whole operation process to carry out movable uniform seed feeding, then the uniformity of the mytilus coruscus seeds in the culture pond is kept, the whole survival rate is improved, and the problem that labor is wasted by manual operation can be solved in the whole operation process.

The invention relates to the field of cell culture and discloses a culture medium for thick-shelled mussel mantle cells and an application thereof. The culture medium comprises a basal culture medium and additives, wherein the additives comprise thick-shelled mussel serum, proanthocyanidins, bovine insulin, growth factors, antibiotics, defensins, mussel mucin and a buffer. The culture medium can be used for in vitro culture of thick-shelled mussel mantle cells and can better maintain the survival and growth of in vitro cultured thick-shelled mussel mantle cells. Defensins in a specific proportion are added to the culture medium, and the best growth and reproduction state can be achieved when the thick-shelled mussel mantle cells are cultured using the culture medium in the specific proportion. The application also provides an application method of the culture medium, which is simple to operate and can produce cultured thick-shelled mussel mantle cells with high cell density, good morphology and high wall adhesion.

The invention provides a method for promoting gonad development of mytilus coruscus, which is characterized in that estradiol is used for promoting gonad development of mytilus coruscus, and the treatment concentration of estradiol is 10-1000 [mu] g / L. Sampling analysis is carried out on the mytilus coruscus in various seasons in the Shengsi squids, the development conditions of different seasonal glands are revealed, the influence of the exogenous estradiol on the development of the gonads of the mytilus coruscus is explored, the spermiation and ovulation of parent mytilus coruscus induced by the estradiol and the fertilization and incubation conditions of larvae are observed, and the deformity rate of D-type larvae is counted. The invention not only provides a theoretical basis for biological characteristics of the mytilus coruscus in the Shengsi squids, but also reveals the important influence of the exogenous estradiol on breeding of the breeding mussels, and provides important theoretical support and practical guidance for sustainable development of the breeding industry of the mytilus coruscus.

The application discloses a mussel polysaccharide and a preparation method and application thereof. The mussel polysaccharide MP-I with a novel structure is prepared from Mytilus coruscus through the process steps of water extraction and concentration, enzymatic deproteinization, alcohol precipitation and anion exchange, and the like. Animal experiments prove that the mussel polysaccharide MP-I prepared by the application can effectively improve intestinal flora and relieve intestinal inflammation by inhibiting the expression of inflammatory signal pathways such as JAK2 / STAT3 and NF-kappa B. Therefore, the mussel polysaccharide MP-I is a natural and safe drug or food for improving inflammatory bowel disease, can effectively improve the inflammatory bowel disease, is safe, non-toxic and free of side effects, the raw material is abundant, the steps are simple, the cost is low, and the effect is remarkable.

The invention discloses a mytilus coruscus byssus adhesion protein mutant, application thereof and a method for identifying phenotypic characters of mytilus coruscus byssus, and relates to the technical field of biology. The amino acid sequence of the mytilus coruscus byssus adhesion protein mutant is as shown in SEQ ID NO. 4. In-depth study shows that non-synonymous mutation of a mytilus coruscus Mfp3-6 gene coding region can directly influence functional characteristics of protein, so that a clear causal relationship is established between a genotype and a mytilus coruscus byssus adhesion performance phenotype. The invention further provides a mytilus coruscus byssus adhesion protein mutant. The mytilus coruscus byssus adhesion protein mutant has an excellent adhesion strength characteristic, so that the mytilus coruscus byssus adhesion protein mutant has a wide application prospect in the field of functional adhesion materials. The protein mutant can be widely applied to development of materials such as medical glue, dressing and gel, provides important technical support for research and development of bionic adhesion technology and novel biological materials, and has good industrialization potential and social value.

The invention belongs to the technical field of biology, and particularly discloses a psychrobacter flexneri strain which is named as Psychrobacter fozii M209, is classified and named as Psychrobacter fozii, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241109. The invention further discloses application of the psychrobacter flexneri M209 in induction of juvenile mollusk attachment of mytilus coruscus, a biofilm formed by the strain has activity of inducing juvenile mollusk attachment of mytilus coruscus and can adapt to extreme environments, and a novel scheme with a wider application range is provided for increasing the attachment rate of mytilus coruscus and increasing the yield of mytilus coruscus.

The invention belongs to the technical field of shellfish genetic breeding, and particularly relates to a cultivation method of triploid offspring seeds for promoting growth of mytilus coruscus. According to the cultivation method, when the proportion of first polar bodies in fertilized eggs of mytilus coruscus accounts for 35%-40% of the total fertilized eggs, a cytochalasin B (CB) solution induces the fertilized eggs of mytilus coruscus, and then incubation and development are carried out. According to the mytilus coruscus triploid offspring seeds cultivated through the cultivation method, the triploid rate can reach 84%, the growth rate is improved by 30%-40% compared with that of diploid, larvae enter an attachment metamorphosis stage in advance, the seedling cultivation cost is greatly reduced, and a new direction is provided for industrialization development of mytilus coruscus.

The invention belongs to the technical field of biological application, and particularly relates to a mytilus coruscus shell length characteristic related SNP molecular marker and application thereof. The nucleotide sequence of the SNP molecular marker related to the shell length characteristic of mytilus coruscus is as shown in SEQ ID NO: 1, and the mutation type is C / A mutation. The development method of the molecular marker comprises the following steps: collecting a mytilus coruscus sample, measuring phenotypic data of the shell length of the sample, combining with whole genome association analysis, screening out SNP candidate sites obviously associated with the shell length characteristic, designing specific primers, and finally determining the associated SNP marker through two-stage analysis of primary screening and cross-population verification by utilizing PCR amplification and a first-generation sequencing technology. According to the invention, a mytilus coruscus shell length characteristic SNP marking system is established, the cost is greatly reduced compared with that of traditional chip detection, and false positive interference is eliminated through large sample verification. The screening and verification method of the SNP molecular marker can be used for molecular marker-assisted breeding, accelerates breeding of improved varieties of mytilus coruscus, and provides technical support for genetic mechanism analysis of growth traits of mytilus coruscus.

The invention relates to the field of seafood processing, and discloses a thick-shell mussel quality maintaining method based on steam and vacuum synergistic heating, which comprises the following steps: S1, pretreatment: firstly selecting fresh thick-shell mussels with the same size, and then taking complete mussel meat by using a meat taking tool; s2, vacuum sealing: putting the shellfish meat taken out in the step S1 into a food-grade retort pouch, and immersing in a steam water bath at 95-100 DEG C, and S3, seasoning and packaging: firstly preparing seasoning liquid, then mixing the seasoning liquid with the shellfish meat cured in the step S2, and soaking for 45-60 minutes, so that the flavor is uniformly permeated; and S4, low-temperature storage and shelf life prediction: establishing a suitable cold chain storage environment by using a refrigerator, and putting the vacuum shellfish meat prepared in the step S3 into the refrigerator for storage. Through a steam vacuum synergistic heating technology, the limitation of a traditional single hot processing technology is effectively overcome, compared with traditional boiling water heating (BH), the juice loss rate is reduced by 30% or above, and the structural integrity of muscle fibers is remarkably improved.

The invention belongs to the technical field of biological application, and particularly relates to a mytilus coruscus body weight character related SNP molecular marker and application thereof. The nucleotide sequence of the SNP molecular marker related to the weight of mytilus coruscus is as shown in SEQ ID NO: 1, and the mutation type is A / G mutation. The development method of the molecular marker comprises the following steps: collecting a mytilus coruscus sample, measuring weight phenotype data of the sample, combining whole genome association analysis, screening out SNP candidate sites obviously associated with the weight character, designing specific primers, and finally determining the associated SNP marker through two-stage analysis of primary screening and cross-population verification by utilizing PCR amplification and a first-generation sequencing technology. The invention focuses on the development of practical molecular markers of weight traits, constructs a multi-geographical population joint analysis population, establishes a standardized weight monitoring specification, and adopts an extreme phenotype grouping strategy to enhance the detection efficacy, thereby identifying the weight trait SNP marker of mytilus coruscus with breeding application value. And core technical support is provided for breeding of high-yield new varieties of mytilus coruscus.

This invention relates to the field of molecular biology, and in particular to a molecular marker, primer pair, and their applications related to mussel species. The invention provides a molecular marker related to mussel species, comprising molecular marker 1 with the nucleotide sequence shown in SEQ ID NO.1 and molecular marker 2 with the nucleotide sequence shown in SEQ ID NO.2, and designs an amplification primer pair based on molecular marker 1 and molecular marker 2. Results from specific embodiments of the invention show that this primer pair can accurately identify *Mussela purpurea*, *Mussela thick-shelled*, and hybrid mussels. Therefore, the molecular marker and primer pair provided by this invention can be used to identify and screen *Mussela purpurea*, *Mussela thick-shelled*, or hybrid mussels of *Mussela purpurea* and *Mussela thick-shelled*, promoting the orderly development of artificial breeding and aquaculture production of mussels.

The invention discloses mussel polysaccharide as well as a preparation method and application thereof. Mytilus coruscus is used as a raw material, the mussel polysaccharide MP-I with a brand-new structure is prepared through the process steps of water extraction and concentration, enzymolysis for deproteinization, alcohol precipitation, anion exchange and the like, and animal experiments prove that the prepared mussel polysaccharide MP-I can effectively improve intestinal flora and improve the intestinal immunity. The intestinal inflammation can be relieved by inhibiting the expression of inflammation signal channels such as JAK2 / STAT3 and NF-kappa B; therefore, the mussel polysaccharide MP-I is a natural and safe medicine or food for improving the inflammatory bowel disease, can effectively improve and verify the inflammatory bowel disease, and is safe, free of toxic and side effects, rich in raw materials, simple in steps, low in cost and remarkable in effect.

The invention relates to the technical field of molecular biology, in particular to a molecular marker related to mussel varieties, a primer pair and application of the molecular marker. The invention provides a molecular marker related to mussel varieties, the molecular marker comprises a molecular marker 1 with a nucleotide sequence as shown in SEQ ID NO.1 and a molecular marker 2 with a nucleotide sequence as shown in SEQ ID NO.2, and an amplification primer pair is designed based on the molecular marker 1 and the molecular marker 2. Results of the specific embodiment of the invention show that the primer pair can be used for accurately identifying common mussels, mytilus coruscus and hybrid mussels. Therefore, the molecular marker and the primer pair provided by the invention can be used for identifying and screening mytilus edulis, mytilus coruscus or hybrid mussels of mytilus edulis and mytilus coruscus, and the ordered development of artificial breeding and breeding production of mussels is promoted.

The invention discloses a mytilus coruscus Wujin shell color related SNP molecular marker and application thereof, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the mutation type is G / A mutation. The molecular marking method comprises the following steps: collecting a mytilus coruscus sample; quantifying the shell color phenotype by using a color measuring instrument; screening out SNP candidate sites significantly associated with the black gold shell color by combining whole genome association analysis GWAS; designing a specific primer, and adopting a PCR amplification and first-generation sequencing technology; through two-stage analysis of preliminary screening and cross-group verification, the stably associated SNP marker is finally determined. A low-cost typing system of the mytilus coruscus black gold shell color SNP marker is established for the first time, the detection cost is greatly reduced compared with that of a traditional chip, and false positive interference is eliminated through large sample verification; the marker can be directly used for molecular marker-assisted breeding, accelerates the breeding of a high-added-value Wujin strain, and provides technical support for the analysis of a genetic mechanism of shell color formation.

The invention relates to the technical field of biology, in particular to mytilus coruscus exosome-like nano-vesicles and application thereof in preparation of osteogenesis products. The functional activity research system of the exosome-like nano vesicles from aquatic products can be improved in the academic level, meanwhile, novel safe and efficient active substances are explored for the bone health field, high-value utilization of shellfish resources can be promoted, the academic value is remarkable, and the method has important industrial application prospects.

The application relates to the field of aquatic product processing equipment, and discloses a device for stripping thick shell mussel columns through water jet flow at low temperature, which comprises an operation table, a sliding rail one and a mounting table are arranged on the operation table, a placing plate is slidably connected to the sliding rail one, a fixing assembly is arranged on the placing plate, a water jet flow stripping mechanism and a wastewater circulating mechanism are arranged in the operation table, an anti-freezing assembly is arranged on the water jet flow stripping mechanism, a displacement mechanism and cooling equipment are arranged on the mounting table, the mounting table is connected with a mounting block through the displacement mechanism, and an angle fine adjustment mechanism is arranged on the mounting block. The high-pressure water jet flow stripping technology is combined with a visual sensor to realize efficient and accurate stripping, the stripping efficiency is improved, the anti-freezing pipe and dynamic heating ensure stable operation of the equipment under a low-temperature environment, the problem of low stripping efficiency caused by water freezing is solved, and water resource saving and operation cost reduction are realized through the wastewater circulating mechanism.

The invention relates to the technical field of molecular biology, in particular to a C-type lectin gene MytiCTL17A and application of coding protein of the C-type lectin gene MytiCTL17A. The invention discovers and verifies that the C-type lectin protein MytiCTL17A from mytilus coruscus has calcium ion dependence, remarkable and broad-spectrum microalgae agglutination activity for the first time. Experiments prove that the protein can effectively agglutinate multiple different microalgae (including chlorella and two kinds of isochrysis) in taxonomy, and an obvious agglutination block mass is formed in a short time. Compared with lectin reported in early literatures, the protein provided by the invention shows excellent broad spectrum, which means that a single preparation can cope with various microalgae problems, and has wider application potential. The protein provides a brand new core material basis for developing a new generation of efficient, environment-friendly and broad-spectrum microalgae treating agent.