61 results about "Mytilus coruscus" patented technology

The invention discloses a preparation method of an antioxidant peptide derived from mytilus coruscus, which comprises the following steps: 1) adding water into mytilus coruscus meat degreased dry powder serving as a raw material; 2) homogenizing materials obtained by the step 1) first, then preheating, adjusting the pH value to 7.0 to 9.0, and adding protease to start an enzymolysis reaction; and3) adjusting the pH value to 6.9 to 7.1 at the end of the enzymolysis reaction, inactivating enzyme, centrifuging, enriching active peptides by ultrafiltration and finally obtaining the antioxidant peptide by concentration and freeze drying. The invention also discloses the use of the antioxidant peptide obtained by the preparation method as an active functional ingredient. The antioxidant peptide obtained by the preparation method has high activity.

The invention provides a method for preparing a marine mussel adhesive protein waterproof biological adhesive, and relates to a biological adhesive. The invention provides the method for preparing marine mussel-Mytilus coruscus adhesive protein waterproof biological adhesive with degradability, water resistance and good biological compatibility by a method of biological gene engineering. The method comprises the following steps: extracting total RNA from the foot part of alive Mytilus coruscus to be reversely transcribed into cDNA; designing a PCR primer according to an Mcfp-1 gene sequence of the Mytilus coruscus; connecting the obtained DNA fragment in a prokaryotic expression vector pGEX-4T through PCR amplification to construct a recombinant plasmid; then transforming the recombined plasmid in a colon bacillus BL21 bacterial strain; and finally, purifying and modifying the expressed polypeptide product. The preparation method is simple, and the expression product is soluble, can be easily purified with low cost, and has similar or better effect compared with CELL-TAK<TM> cell and tissue adhesive without cytotoxicity. The adhesive accords with the requirement of scientific research, and can be used as a biological medical material.

A method for artificial oxytocin and fertilization of Mytilus coruscus comprises the following steps of a parent cultivating pool: building an outdoor cultivating cement pool with the dimension of 10cmx1.5mx1m, stocking inflatable stones, covering canvas above the cement pool to prevent rain and sunlight, and shading the periphery of the cement pool by dark cloth to regulate strength of light; selecting Mytilus coruscus which has new and integral shell surfaces, full gonads, complete gill, light red ovaries and white testes, and is free of damage and powerful in closure of double shells; preparing baits, wherein chlamydomonas live baits including cultured Dunaliella viridis, latymonas subcordiformis and Chaetoceros serve as primary baits, and artificial compound feed including spirulina powder, chlorella powder and yeast tablets serves as secondary baits; drying parent Mytilus coruscus with developed gonads in the shade for 6-8 hours, stimulating the parent parent Mytilus coruscus for 1 hour through running water, and feeding the parent parent Mytilus coruscus into prepared warming seawater.

The invention relates to an artificial spawning and cultivation method for mytilus coruscus. The method comprises the following steps of parent selection, intensified parent cultivation, artificial acceleration of maturation, artificial spawning, larvae rearing, larva cultivation and juvenile mytilus coruscus cultivation. The artificial spawning and cultivation method has the advantages that the season limit on mytilus coruscus reproduction is broken through, the time limit on mytilus coruscus reproduction is avoided, and the artificial spawning and cultivation method is of great significance in developing juvenile mollusk production and sustainable cultivation of economic mussels such as mytilus coruscus. On the basis of existing artificial cultivation technology for mytilus coruscus, through further study, experiment parameters are optimized for control over artificial spawning and larvae hatching conditions in the experiment process, the cultivation technology is more precise, and the cultivation hatchability and young mytilus coruscus survival rate are improved.

The invention discloses a mytilus coruscus half-shelf removal device, which comprises a processing bench, a shell opening device, a posterior adductor cutting device, a mytilus coruscus fixing device, a half-shell removal device and a control and pneumatic system portion. The processing bench plays a role in supporting; the shell opening device forces mytilus coruscus to open by a certain width through a wedged tool; the posterior adductor cutting device cuts off posterior adductor of the mytilus coruscus through a concave cutter; the mytilus coruscus fixing device is used for fixing the mytilus coruscus through a mytilus coruscus fixing table; the half-shelf removal device enables a gas nozzle to jet high-pressure impact gas to realize impact shell removal; the control and pneumatic system portion provides power for feeding and retreating of the wedged tool and the concave tool, provides the high-pressure impact gas for the gas nozzle and controls each electromagnetic valve to control the mytilus coruscus half-shelf removal device to operate. By the mytilus coruscus half-shelf removal device, mechanical half-shelf removal of the mytilus coruscus is realized, manpower and cost are saved, and efficiency is improved.

The invention discloses a mytilus coruscus antibacterial peptide. The name of the antibacterial peptide is myticin, and the open reading frame of cDNA gene coded by the antibacterial peptide has a special nucleotide sequence. The invention also discloses a preparation method and a eukaryotic expression vector of the mytilus coruscus antibacterial peptide and application thereof in antibiotic preparation. The antibacterial peptide provided by the invention can be used as a feed additive to substitute some antibiotics in the traditional feed and used in the aquaculture industry, thereby realizing green culture, reducing or even eliminating the accumulation of antibiotic in aquatic products, and improving the quality of the aquatic products; and the antibacterial peptide also can be used as novel biological antibiotic and used for treatment or prevention of bacterial disease.

The invention discloses a method for high efficient preparation of novel Mytilus coruscus antimicrobial peptide Mytichitin-A in pichia pastoris. A gene engineering method is adopted and the full-length gene of the antimicrobial peptide Mytichitin-A is inserted into an exosome type expression vector pPICZalphaA through EcoRI and KpnI restriction enzyme cutting sites, so as to obtain a pPICZalphaA-Mytichitin-A recombinant expression vector. Through an electrotransformation method, a SacI enzyme linearized recombinant plasmid pPICZalphaA-Mytichitin-A is transferred into the pichia pastoris GS115. Through optimizing, the optimum expression conditions are determined as follows: methanol concentration is 1.0%, fermentation time is 96h, and the yield is 45.5 microgram/mL. The result of bacteriostasis experiments shows that the recombinant antibacterial peptide has an obvious inhibition effect on both staphylococcus aureus and bacillus subtilis 151-1.

The invention provides a method for artificially protecting the seedlings of the mytilus coruscus. The method comprises the following steps of: putting the juvenile mytilus coruscus into a net piece bag in the early temporary cultivation, suspending the net piece bag into the sea water with the depth of 3+/-0.4m, and leading the net piece bag to have no adherent sludge and the other attachments; dispersing the mytilus coruscus seedlings which are adhered to one another after the temporary cultivation, washing off the floating sludge out of the mytilus coruscus seedlings and the harmful livingbeings, and packing the seedlings, wherein the density is thick rather than overlapping, and the pressure of the net piece to the juvenile seedlings is controlled such that the juvenile seedlings arenot accumulated in the net piece bag when the seedlings are packed; and removing the net piece after the seedlings are packed, continuously performing the later temporary cultivation, and harvesting the seedlings after the temporary cultivation. The method for artificially protecting the seedlings of the mytilus coruscus is preformed in the sea water with a proper depth, and leads the juvenile seedlings to grow in the environment which is smooth in water flow and small in sludge and miscellaneous algae disoperation by measures, so that the survival rate is more than 50%, specifically the survival rate is improved by at least 8% compared with the prior art.

The invention provides an automatic processing production line for Mytilus coruscus, belongs to the technical field of fishery tools, and solves the technical problems of high labor intensity and lowefficiency in existing Mytilus processing. The automatic processing production line for the Mytilus coruscus comprises a conveyor belt and a driving motor driving the conveyor belt to operate, whereinan inclined directional conveying device is arranged on the side, close to an input end, of the conveyor belt; multiple fixing boxes capable of accommodating and fixing the Mytilus coruscus are placed on the conveyor belt at intervals, a meat removing device is arranged on the side, close to an output end, of the conveyor belt, a shell opening device is also arranged between the directional conveying device and the meat removing device, the shell opening device comprises a shell opening tool and a cut-off tool which are arranged vertically downwards, and the shell opening tool and the cut-offtool are arranged in the width direction of the conveyor belt side by side at an interval; and the meat removing device comprises a jet nozzle for jetting high-pressure water flows. The automatic processing production line is high in automation degree and high in work efficiency.

The invention belongs to the field of aquatic product biogenetic engineering, and discloses an electroporation transfection method of eyespot larvae of mytilus coruscus. siRNA is designed according toa full-length sequence of thyroid hormone receptor genes of the mytilus coruscus obtained by clone, and different parameter settings are adopted to analyze the knock reduction situations of differentpulse patterns on the thyroid hormone receptor genes; the larvae after 96 hours of electroporation transfection are collected, and larvae RNA is extracted; and thyroid hormone receptor gene primers are designed, and the expression situation of the thyroid hormone receptor genes is detected by a fluorogenic quantitative PCR. According to the electroporation transfection method of the eyespot larvae of the mytilus coruscus, compared with the treatment without siRNA and non-sense siRNA, results show that the addition of the thyroid hormone receptor gene siRNA significantly reduces the expressionquantity of the genes, and the knock reduction efficiency is nearly 100%.

The invention discloses a compound shellfish modified biological phosphorus removal material and a preparation method and application thereof. In the method, mytilus coruscus shells, oyster shells and scallop shells are taken as raw materials and smashed according to a certain proportion, and the smashed raw materials are subjected to preheating treatment, calcining treatment and heat-preserving treatment to obtain a powdery compound shellfish modified biological phosphorus removal material, and the phosphorus removal material is applied to phosphorus removal of wastewater. The compound shellfish modified biological phosphorus removal material has the advantages of simple preparation process, realization of recycling of waste resources, and certain social, economic and environmental benefits. The prepared modified biological phosphorus removal material has a removal rate of 90 percent or more for phosphorus in water bodies, and has very high phosphorus removal and water purification capabilities. The phosphorus removal effect and the maintenance time can be improved remarkably in an adding way that the modified biological phosphorus removal material is wrapped with a slow-release package, and the slow-release package is added into a water body.

The invention discloses a method for cultivating nuclear free marine rainbow pearls with mytilus coruscus. The method comprises the following steps: S1, selection of the mytilus coruscus for pearl cultivation; S2, donor sheet selection: making pearl nuclei of various small shapes by using shells and adhering mantle pieces cut from the similar mytilus coruscus onto each of the pearl nuclei; S3, operation and postoperative treatment: inserting the pearl nuclei together with the mantle pieces into mantles on the two sides of a mytilus coruscus body and/or a genital gland by using a nucleus implanting tool and hanging the mytilus coruscus into sea for quiet cultivation after the completion of pearl nucleus inserting operation. The pear cultivating method disclosed by the invention fills up the blank of pearl cultivation with the mytilus coruscus in the world, is low in investment cost, can cultivate without a cage, is large in economic development potential and achieves the purpose of production demands of farmers due to breeding twice a year; the pearls are natural, colorful and black and can be directly used for jewelry making.

The invention discloses a method for improving frozen storage quality of frozen aquatic products. The method comprises the following steps: (1) preparing degreased mytilus coruscus powder; (2) addingcompound proteinase for hydrolization; (3) performing refining; and (4) performing freezing fresh keeping. The method has the beneficial effects that the raw materials for the method are selected fromnatural substances or substances easy to degrade, so that toxic substances are not remained; based on three measures of a bacteriostat, superhigh pressure and ultralow temperature freezing, the content of harmful microorganisms in the meat quality of the aquatic products is very low, so that the condition that the aquatic products are putrid and deteriorated due to the microorganisms is effectively delayed; and through combined use of active polypeptides, a fresh keeping agent and quick freezing, the deteriorating speed of protein in the aquatic products can be effectively reduced to the lowest; after the aquatic products are subjected to frozen storage for a long term, the mouth feel, the water retention property, the tender taste, the gelation property and the nutrient value of the meatquality of the aquatic products can also be preserved; and the method is capable of improving the frozen storage quality of the frozen aquatic products efficiently and safely.

The invention provides a artificial breeding method for Mytilus coruscus, which comprises the step of larval breeding with the water temperature of 14-21 DEG C and the salinity of 25-28. Water is changed once through siphonage or a method of discharging water from the bottom of a pool in the morning every day, and the total water change volume is gradually increased from 30 percent to 50 percent; bait is taken as algae and fed twice every day, namely, being fed once after water change in the morning, and being fed once more in the evening according to the density of algae in the cultivating water body, so as to satisfy the requirement on algae density. The method provided by the invention has the advantages that as the prior art is further researched, the requirement on water temperature in the larval breeding step is decreased, and the breeding process is more reasonable by optimizing other parameters, therefore, compared with the prior art, the energy consumption is low, and the bred young Mytilus coruscus is strong.

The invention provides an automatic shelling device of mytilus coruscus. The automatic shelling device comprises a feeding hole, a controller, a machine base, a first motor, a first linear motor, a first movable block, a first fisheye bearing, a first expansion plate, a supporting plate, a channel, an electrically operated valve, a second fisheye bearing, a first drainage valve, a first water outlet pipe, a second expansion plate, a baffle plate, a rotary shaft, a flange bearing, a mesh plate, a first case, a water inlet valve, a water inlet pipe, a rack, a second motor, a second gear, a second case, a vibrating plate, a third fisheye bearing, a shock insulation plate, a vibrating motor, a second drainage valve, a second water outlet pipe, a chain, a wheel stand, a first gear, a first double-head air cylinder, a second linear motor, a second movable block, a third double-head air cylinder, a second high-pressure nozzle, a third high-pressure nozzle, a second double-head air cylinder, a first high-pressure nozzle, a through hole, a case door, a frame and supporting legs, wherein the first case is mounted below the rack; the feeding hole is mounted on the first case; the water inlet pipe is mounted on the first case. The automatic shelling device has the advantage that the positions of the nozzles can be adjusted.

The invention discloses a cultivation technology for planktonic larvae of mytilus coruscus, and relates to artificial shellfish cultivation. The cultivation technology for the planktonic larvae of the mytilus coruscus comprises the following steps: selecting a cultivation time, selecting a cultivation sea area, building an outdoor cultivation pond, cultivating the planktonic larvae, performing cultivation condition management, and performing feeding management. According to the cultivation technology, the survival rate of the planktonic larvae is increased, spat bodies are enlarged, a problem of two 'dangerous periods of spat sinking' ubiquitous in artificial cultivation is solved through a research, a technical problem is solved, and the cultivation risk is lowered.

The invention discloses a mytilus coruscus multi-drug resistance protein Abcc. The multi-drug resistance protein Abcc is a cloned gene, which is obtained by cloning a gene core fragment of mytilus coruscus Abcc by a gene cloning technology. The invention also discloses a novel marine biological pollution detection marker, which uses mytilus coruscus as a biological sample for detecting marine pollution, and uses the mytilus coruscus multi-drug resistance protein Abcc as a detection marker for marine pollution. The relative expression amount of a multi-drug resistance protein Abcc gene of the mytilus coruscus multi-drug resistance protein Abcc is the highest in digestive glands and gills, and a PCR reaction system for cloning can ensure the specificity of an amplification reaction and improve the PCR amplification efficiency. The detection marker provided by the invention has strong specificity, can directly take target cells or target molecules in organisms as reaction endpoints to carry out sensitive biological reactions at the molecular level, and has an early warning effect on marine pollutant exposure and toxicity effects.

The invention relates to a usage of potassium ions in promoting the metamorphosis of mytilus coruscus larvae and shortening the seed settlement time of the mytilus coruscus larvae and further relates to a usage of a potassium ion antagonist in inhibiting the metamorphosis of the mytilus coruscus larvae and a method for promoting or inhibiting the metamorphosis of the mytilus coruscus larvae. The usage and the method have the advantages that evidences prove that by the potassium ions, the metamorphosis rate of the mytilus coruscus larvae can be improved, and the seed settlement time of the mytilus coruscus larvae can be shortened, so that the potassium ions can be used for increasing the production success rate of mytilus coruscus seeds and shortening the production period; and meanwhile, cheap and nontoxic sylvite is suitable for large-scale seed production. The evidences also prove that the potassium ion antagonist can inhibit the metamorphosis of the mytilus coruscus larvae, and a novel way is provided to control marine contamination. The conclusion of the study is helpful in stating the settlement and metamorphosis mechanism of mytilus coruscus.

The invention provides an application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion, and the method for reducing juvenile mytilus coruscus adhesion by 2 (5H)-furanone comprises the following steps: carrying out enlarged culture on bacteria in a liquid culture medium to obtain an initial bacterial stock solution;after centrifuging, discarding the supernatant, mixing with sterilized filtered seawater, and resuspending to obtain a bacterial solution from which the culture medium is washed away; adding the bacterial liquid and 2 (5H)-furanone into a sterile culture dish containing a sterilized slide for bacterial microorganism coating culture, then adding sterilized filtered seawater to wash the slide to obtain a bacterial microorganism coating, transferring the bacterial microorganism coating into the sterile culture dish, and adding mytilus coruscus juvenile mollusk; according to the method, the quorumsensing inhibitor 2 (5H)-furanone is used for interfering with microbial film formation such as EPS biomass and a bacterial metabolism process so that attachment of mytilus coruscus juvenile mollusksis remarkably reduced, and a basis is provided for the field of biological pollution prevention.

The invention discloses a handheld tool and a method for measuring mytilus coruscus gould. A measuring body is arranged on the upper portion of the integrally T-shaped handheld tool, and a handle is arranged on the lower portion of the handheld tool and is fixedly connected with the measuring body. The handheld tool is characterized in that an LED display screen is arranged on the front surface of a main shell, four bulbs are arranged at four corners of the back surface of the main shell, a miniature camera is arranged in the center of the back surface of the main shell, a sonar receiving and transmitting device is further mounted on the back surface of the main shell, and the miniature camera is positioned in the center of the sonar receiving and transmitting device; a size measuring switch, a distance measuring switch, a light switch and an automatic monitoring switch are arranged on the front surface of a handle shell, a power switch is arranged in a side surface of the handle shell, and a battery charging hole is formed in the bottom of the handle shell; a lithium battery and a computer chip are arranged inside the handle. The handheld tool and the method have the advantages that the sizes of the underwater mytilus coruscus gould can be efficiently measured by the aid of the handheld tool, and the handheld tool can be used as an auxiliary tool for investigating and evaluating the mytilus coruscus gould in natural environments; the sizes of the mytilus coruscus gould can be easily, accurately and effectively measured by the aid of the handheld tool.

The invention provides an application of shell powder of mytilus coruscus in the aspect of bacteriostasis of marine bacteria, which comprises the following steps: cleaning, drying, grinding and calcining waste shells of mytilus coruscus to obtain the shell powder; inoculating marine bacteria to be detected into the liquid culture medium for activation, then adding the shell powder into the liquidculture medium for reaction, and sterilizing the reaction product for later use In the invention, the shell powder disclosed by the invention has different antibacterial effects on shewanella, vibriosplendidus and pseudoalteromonas at different concentrations, and the 24-hour antibacterial rates respectively reach 93.6%, 92.1% and 95.7%; the antibacterial effects of the calcined shell powder on shewanella bacteria and vibrio splendidus tend to be stable within 3 hours and 6 hours respectively, and the antibacterial rates of the shell powder are not remarkably different along with the increaseof time; therefore, the shell powder of the mytilus coruscus has antibacterial activity on certain marine bacteria, the antibacterial effect can tend to be stable in a short time, and a new directionis provided for resource utilization of shells of waste mytilus coruscus.

The invention discloses deep-sea bacillus which has the preservation number of CCTCC M 2018065, and is classified and named as Bacillus sp. A microbial envelope formed by bacteria has a relatively high induction activity on the adhesion of a juvenile mollusk of mytilus crassitesta. The invention further relates to application of the deep-sea bacillus to inducing the adhesion of the juvenile mollusk of the mytilus crassitesta.

The invention provides cytochrome P450 enzyme CYP450. The nucleotide sequence of a gene of the cytochrome P450 enzyme CYP450 is SEQID NO.1, and the amino acid sequence of the cytochrome P450 enzyme CYP450 is SEQID NO.2. The invention also provides mytilus coruscus cytochrome P450 enzyme CYP450 as a novel marine organism pollution detection marker, and a method for detecting marine organism pollution. The novel marine organism pollution detection marker disclosed by the invention has the beneficial effects that the mytilus coruscus cytochrome P450 enzyme CYP450 is used as a marine pollution detection marker, is high in sensitivity and easy to detect and analyze, and can also be used for artificial breeding of mytilus coruscus, diseases and water quality deterioration can be discovered timely, generation of red tides can be prevented, and economic losses are reduced; and after the sea is polluted, the cytochrome P450 enzyme CYP450 has notable effects in the oxidation process, the contentof the cytochrome P450 enzyme CYP450 is notably increased, and through the changes, marine pollution can be effectively detected.

The invention provides a method for artificial propagation of mytilus coruscus in an intertidal zone of a rocky reef phase. According to the invention, mytilus coruscus juveniles are temporarily conserved at a sea area; the mytilus coruscus larvae are wrapped in net bags prepared with a papyrus material; the net bags with the mytilus coruscus larvae are connected in series, and are placed into the intertidal zones of the rocky reef phase or a dimension stone phase of a natural sea area, such that the artificial propagation of the mytilus coruscus is completed. According to the invention, thick hemp ropes are selected as an attachment base, and thin hemp ropes are wound on the thick manila ropes, such that the base surface area to which mytilus coruscus byssuses are attached is increased. The net bags used for wrapping the mytilus coruscus larvae and the hemp ropes used for attachment of the mytilus coruscus larvae can be rapidly digested under seawater erosion, such that artificial removal is not needed. Therefore, manual labor is effectively reduced, and plastic garbage is not brought to the environment. Mytilus coruscus can first attach to the net bags or the hemp ropes, and with the digestion of the net bags and the hemp ropes, the mytilus coruscus can attach to rocky reef gaps. With the mytilus coruscus secondary attachment manner, mytilus coruscus larvae attachment rate on the rocky reef is greatly improved, and the mytilus coruscus artificial propagation effect is improved.