Use of 3, 4 dihydro-8-hydroxy-1-hydro-2-benzopyran 1 derivative for inhibiting plant pathogenic fungus

A technology of inhibition and germs, applied in the field of antibacterial substances, can solve the problems of single species, few types of biological fungicides, and inability to prevent and control various plant fungal diseases, and achieve the effect of convenient use and strong antibacterial activity

Inactive Publication Date: 2006-05-24
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytopathogenic fungi are important pathogens that cause a variety of plant diseases. At present, many antibacterial substances produced by microbial metabolism have been used in production and have made a great contribution to alleviating the pollution of agricultural products caused by chemical pesticides. However, due to biological fungicides There are few types and a single variety, which is far from meeting the needs of preventing and controlling various plant fungal diseases, especially related to Phytophthora phytophthora of the present invention, and there is no effective biological fungicide to control it at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inhibition of Phytophthora ramie

[0024] The PDA medium prepared with different concentrations of benzopyran derivatives had different inhibition rates on the mycelial growth of Phytophthora ramie, as shown in Table 2. It can be seen from the table that when the concentration is 0.3 μg / ml-0.8 μg / ml, its inhibitory effect reaches 55.98%-100%. Taking the logarithm of different treatment concentrations in Table 2 as the abscissa and the inhibition rate as the ordinate, the linear regression equation can be obtained as: Y=97.902X+109.05. In the formula, Y represents the mycelia inhibition rate; X represents the concentration logarithm, and obtains the middle concentration (EC) of the inhibition of benzopyran derivatives to Phytophthora ramie mycelium by the regression equation 50 ) is 0.25 μg / ml. EC 50 Indicates the concentration of benzopyran derivatives when the inhibition rate reaches 50%. Therefore, the ideal inhibitory effect can be achieved at a concentration of ...

Embodiment 2

[0027] Inhibition of Cucumber Phytophthora

[0028] The PDA medium made of different concentrations of benzopyran derivatives has different inhibition rates on the mycelial growth of Phytophthora cucumber, as shown in Table 3. It can be seen from the table that the concentration ranges from 0.4 μg / ml to 1.0 μg / ml When, its inhibitory effect reaches 57.91% to 100%. Taking the logarithm of the different treatment concentrations in Table 3 as the abscissa, and the inhibition rate as the ordinate, the regression equation is: Y=106.23X+98.371, and the effect of the benzopyran derivatives on Phytophthora cucumber is obtained by the regression equation. Intermediate Inhibitory Concentration (EC 50 ) is 0.35 μg / ml. Therefore, its inhibitory effect is better when the concentration is 0.30μg / ml-1.0μg / ml.

[0029] Treatment concentration (μg / ml)

Embodiment 3

[0031] Inhibitory Effect on Apple Spotted Deciduous Fungus

[0032] The toxic PDA medium made of different concentrations of benzopyran derivatives had different inhibition rates on the mycelial growth of P. It can be seen from the table that when the concentration is 1.0 μg / ml~10 μg / ml, its inhibitory effect reaches 56.48%~100%. Taking the logarithm of different treatment concentrations in table 4 as the abscissa, the inhibition rate as the ordinate obtains the regression equation as Y=42.352X+58.725, and obtains the middle concentration of the inhibition of benzopyran derivatives to the apple spot leaf fall bacterium by the regression equation (EC 50 ) is 0.62μg / ml, so the inhibitory effect is ideal when the concentration is 0.50μg / ml~10μg / ml.

[0033] Treatment concentration (μg / ml)

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PUM

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Abstract

The invention relates to the application of 3, 4dihydro 8 hydroxyl 1 benzopyrane 1 ramification extracted from Beijing mutation metabolite of Xenorhabdus nematophila pathogenic bacillus to prohibiting plant pathogenic fungi, dissolving the benzopyrane ramification freeze dried pure products in axenic water, mixing into a mother solution in 1000 mug / ml, then separately using PDA culture medium to dilute the mother solution into solutions in 0.20 mug / ml-20 mug / ml. The benzopyrane ramification extracting technique is simple and the produced bacteria are easy to culture in batches; the benzopyrane ramification has stronger bacteriostatic activity to various plant pathogenic fungi, especially to plant Phytophthora palmi; and this compound is firstly used to prohibit plant pathogenic fungi, and its bacteriostatic activity is strong, and it is convenient and safe to use.

Description

technical field [0001] The invention relates to the use of antibacterial substances produced by Xenorhabdus nematophila var.pekingensis metabolism. In particular, it relates to the application in the suppression of phytopathogenic fungi. Background technique [0002] 3,4 dihydro, 8 hydroxy, 1 hydrogen isolated from metabolites of Xenorhabdus nemotophilus var. 2 Benzopyran 1 derivatives-Xenocoumacinsl, this compound Gregson et al. declared a new structure patent in 1989 (WO86 / 01509, United state patent 4,837,222), and its structural formula is C 22 h 34 N 5 o 6 , Carry out this compound in the patent to Escherichia coli (Escbericbia coli), Pseudomonas aeruginosa (Pseudomonasaeruginosa), Lu Shi Acinetobacter (Acinetobacter lwoffi), gigantic bacillus kernel (Bacillus megaterium), dry mycobacterium (Corynebacterium xerosis), Sarcina lutea, Staphylococcus aureus, Streptococcus pyogenes, Streptococcus sanguis and other bacteria, but not against phytopathogenic fungi Inhibiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/76A01N63/02C12N1/20C12P17/06
Inventor 杨秀芬杨怀文简恒朱昌雄刘峥黄武仁袁京京
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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