Photosynthetic bacterium rhodobacter sphaeroides strain, bacterial drug agent, and preparation method and application of bacterial drug agent

A technology of Rhodobacter sphaeroides and photosynthetic bacteria, which is applied in the field of bacterial agents and its preparation, and in the field of photosynthetic bacteria Rhodobacter sphaeroides strains, can solve the problems of high cost and difficulty in the prevention and treatment of rice bacterial streak disease, and achieve low cost, Not easy to drug resistance, simple cultivation process

Active Publication Date: 2019-09-06
HUNAN PLANT PROTECTION INST
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  • Abstract
  • Description
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Problems solved by technology

[0005] The invention provides a photosynthetic bacterium Rhodobacter sphaeroides strain, a bacterial agent and its preparation method and applicat

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  • Photosynthetic bacterium rhodobacter sphaeroides strain, bacterial drug agent, and preparation method and application of bacterial drug agent
  • Photosynthetic bacterium rhodobacter sphaeroides strain, bacterial drug agent, and preparation method and application of bacterial drug agent
  • Photosynthetic bacterium rhodobacter sphaeroides strain, bacterial drug agent, and preparation method and application of bacterial drug agent

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[0036] According to another aspect of the present invention, also provide a kind of preparation method of the biocontrol agent of photosynthetic bacterium Rhodobacter sphaeroides strain as above-mentioned, comprise the following steps: Cultivate by multi-layer plate culture method until red single colony appears; S2, seed culture: insert the red single colony cultivated in step S1 into a serum bottle, and carry out seed culture with photosynthetic bacteria liquid medium to the logarithmic growth phase to obtain Bacteria liquid; S3, production cultivation: inoculate the bacterium liquid in the step S2 by the inoculation amount of 3%~6% of the total volume of the photosynthetic bacteria strain production medium, cultivate to the logarithmic growth phase to obtain the biocontrol of the photosynthetic bacteria Rhodobacter sphaeroides strain Bacteria.

[0037] In this embodiment, the temperature for seed cultivation in step S2 is 30°C to 35°C, the light conditions are 2500Lx to 400...

Embodiment 1

[0054] The inhibitory effect of the photosynthetic bacteria strain Rhodobacter sphaeroides A13 on the pathogenic bacteria causing rice bacterial stripe disease was measured by the inhibition zone method. Take 0.1ml of pathogenic bacteria and smear it on the LB plate, put a sterilizing steel ring in the center, and Add 0.05ml of photosynthetic bacteria solution to the steel ring, incubate at 30°C for 7 days, and observe whether there is a zone of inhibition.

Embodiment 2

[0056] Under greenhouse conditions, the 5×10 6 cfu / mL photosynthetic bacterium Rhodobacter sphaeroides A13 strain biocontrol fungus Soak the rice seeds germinated to white at 30°C for 1 hour, sow the treated rice seeds in pots, and keep the seedlings at 28°C during the identification period. Cultivate in a greenhouse under 10h / d light conditions, and record the plant height regularly. Three representative plants were selected to measure the plant height and wet weight respectively. After the rice enters the seedling stage, the rice seedlings are inoculated with Xanthomonas oryzae, and the disease index is investigated 7 days after the inoculation and the control effect is calculated.

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Abstract

The invention discloses a photosynthetic bacterium rhodobacter sphaeroides strain, a bacterial drug agent, and a preparation method and an application of the bacterial drug agent. The photosynthetic bacterium rhodobacter sphaeroides strain is rhodobacter sphaeroides A13, is preserved in China Center for Type Culture Collection and has the preservation number of CCTCC No:M 2018605. The photosynthetic bacterium rhodobacter sphaeroides strain, the rhodobacter sphaeroides A13, has the advantages of simple cultivation process, short cultivation time and low cost; and a biocontrol agent and a biocontrol fermentation liquid for prevention and control of paddy rice bacterial leaf streak can be prepared from the photosynthetic bacterium rhodobacter sphaeroides strain by the simple, fast and low-cost operation method.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a photosynthetic bacterium Rhodobacter sphaeroides strain. In addition, the present invention also relates to a microbial agent comprising the photosynthetic bacterium Rhodobacter sphaeroides strain and its preparation method and application. Background technique [0002] Bacterial spot disease of rice, also known as thin streak disease and streak disease, mainly damages leaves. The lesions are small dark green water-soaked spots at first, and soon expand into dark green to yellowish-brown thin stripes between the veins, with a size of about 1×10mm, and the two ends of the lesions are infiltrating green. A large amount of beaded yellow bacterial pus often overflows from the lesions, which are small colloidal particles after drying. When the disease is severe, the streaks merge into irregular yellowish-brown to withered white spots, which are similar to bacterial b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P21/00C12R1/01
CPCC12N1/20C12R2001/01C12N1/205A01N63/10
Inventor 程菊娥杜晓华苏品刘勇张德咏戴建平周波郑立敏王忠勇
Owner HUNAN PLANT PROTECTION INST
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