Method for producing DHA and astaxanthin by mixed culture of Schizochytrium and Haematococcus pluvialis
A technology for Haematococcus pluvialis and Schizochytrium algae, which is applied in the field of microorganisms, can solve the problems of complex process, water environment pollution, non-reusable culture medium, etc., and achieves the effect of simplifying the culture process and reducing the culture cost.
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Embodiment 1
[0027] (1) Cultivation of Haematococcus Pluvialls
[0028] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.
[0029] Haematococcus pluvialis was inoculated into the BBM culture medium for the cultivation and expansion of the algae species. The culture conditions for the growth stage of Haematococcus pluvialis were temperature 23°C, light intensity 1200lx, light time 14h / day, culture medium pH 8.0, culture During the process, carbon dioxide is used to control the pH of the culture solution to not exceed 9.
[0030] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin ...
Embodiment 2
[0039] (1) Cultivation of Haematococcus Pluvialls
[0040] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.
[0041] Haematococcus pluvialis was inoculated into the BBM culture medium for cultivation and expansion of algae species. The culture conditions of Haematococcus pluvialis growth stage were: temperature 23°C, light intensity 1500lx, light time 12h / day, culture medium pH 7, culture During the process, hydrochloric acid is used to control the pH of the culture solution to not exceed 9.
[0042] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin 0.012g / L.
[...
Embodiment 3
[0052] (1) Cultivation of Haematococcus Pluvialls
[0053] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.
[0054] Haematococcus pluvialis was inoculated into BBM culture medium for cultivation and expansion of algae species. The culture conditions of Haematococcus pluvialis growth stage were: temperature 28°C, light intensity 2000lx, light time 12h / day, culture medium pH 8.0, During the cultivation process, acetic acid was used to control the pH of the culture solution to not exceed 9.
[0055] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin 0.012g / L.
[005...
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