Method for producing DHA and astaxanthin by mixed culture of Schizochytrium and Haematococcus pluvialis

A technology for Haematococcus pluvialis and Schizochytrium algae, which is applied in the field of microorganisms, can solve the problems of complex process, water environment pollution, non-reusable culture medium, etc., and achieves the effect of simplifying the culture process and reducing the culture cost.

Active Publication Date: 2021-07-09
CABIO BIOTECH WUHAN CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of the two-step method is to solve the contradiction between the growth and reproduction of Haematococcus cells and the different conditions required for spore formation and accumulation of astaxanthin. The disadvantages are (1) complex process; (2) measures to induce astaxanthin accumulation The culture medium needs to be replaced, so the harvested culture medium cannot be reused, resulting in a large amount of waste water being discharged during the production process, which has a polluting effect on the water environment

Method used

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  • Method for producing DHA and astaxanthin by mixed culture of Schizochytrium and Haematococcus pluvialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Cultivation of Haematococcus Pluvialls

[0028] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.

[0029] Haematococcus pluvialis was inoculated into the BBM culture medium for the cultivation and expansion of the algae species. The culture conditions for the growth stage of Haematococcus pluvialis were temperature 23°C, light intensity 1200lx, light time 14h / day, culture medium pH 8.0, culture During the process, carbon dioxide is used to control the pH of the culture solution to not exceed 9.

[0030] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin ...

Embodiment 2

[0039] (1) Cultivation of Haematococcus Pluvialls

[0040] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.

[0041] Haematococcus pluvialis was inoculated into the BBM culture medium for cultivation and expansion of algae species. The culture conditions of Haematococcus pluvialis growth stage were: temperature 23°C, light intensity 1500lx, light time 12h / day, culture medium pH 7, culture During the process, hydrochloric acid is used to control the pH of the culture solution to not exceed 9.

[0042] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin 0.012g / L.

[...

Embodiment 3

[0052] (1) Cultivation of Haematococcus Pluvialls

[0053] Haematococcus pluvialis was purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, number FACHB-1927.

[0054] Haematococcus pluvialis was inoculated into BBM culture medium for cultivation and expansion of algae species. The culture conditions of Haematococcus pluvialis growth stage were: temperature 28°C, light intensity 2000lx, light time 12h / day, culture medium pH 8.0, During the cultivation process, acetic acid was used to control the pH of the culture solution to not exceed 9.

[0055] The formula of BBM culture medium is: NaNO 3 0.25g / L, MgSO 4 ·7H 2 O 0.14g / L, KH 2 PO 4 0.02g / L, CaCl 2 0.02g / L, EDTA 0.0004g / L, FeSO 4 ·7H 2 O 0.002g / L, ZnSO 4 ·7H 2 O 0.002g / L, MnCl 2 4H 2 O0.00004g / L, CoNO 3 ·6H 2 O 0.00006g / L, KOH 0.08g / L, H 3 BO 3 0.0001g / L, CuSO 4 ·5H 2 O0.00003g / L, MoO 3 0.00001g / L, vitamin B 12 0.00003g / L, NaHCO 3 0.06g / L, ampicillin 0.012g / L.

[005...

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Abstract

The invention discloses a method for producing DHA and astaxanthin by mixed culturing Schizochytrium and Haematococcus pluvialls, inoculating Schizochytrium in the fermentation liquid after the growth period of Haematococcus pluvialls to carry out mixed bacterial fermentation culture , Supplement carbon source and sodium glutamate during the fermentation process, and extract oil after the cultivation is completed. The invention has the characteristics of simplifying the cultivation process, reducing the production cost, improving the stability of oil extraction and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms. More specifically, the present invention relates to a method for producing DHA and astaxanthin by mixing Schizochytrium and Haematococcus pluvialis. Background technique [0002] Astaxanthin is a member of the Qinjiaohuang family of the carotenoid family. Qinjiaohuang helps to prevent the oxidation of vitamin A, vitamin E and other carotenoids. It has the strongest antioxidant effect among all carotenoids, 10 times stronger than β-carotene and 100 times stronger than vitamin E. It has been confirmed by nutritionists that astaxanthin can cross the barrier from blood to brain, so astaxanthin can protect the brain, central nervous system and eyes. [0003] Haematococcus pluvialis cultivation method is currently the most important production method of natural astaxanthin. Astaxanthin prepared from microalgae such as Haematococcus pluvialis is in 3S, 3'S configuration, with high antioxidant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P23/00C12P7/64C12R1/89
CPCC12P7/6427C12P23/00C12P39/00
Inventor 汪志明李翔宇余超熊淑婷陆姝欢
Owner CABIO BIOTECH WUHAN CO LTD
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