Culture method of cordyceps militaris
A technology of Cordyceps militaris and a cultivation method, applied in the field of cultivating Cordyceps militaris, can solve problems such as unstable yield and quality, and achieve the effects of tidy growth of fruiting bodies, shortened growth cycle, and fast germination.
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Embodiment 1
[0039] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:
[0040] Step 1: Activation of the strains of Cordyceps militaris
[0041] (1) Formula of mother seed medium:
[0042] Potato 180g, glucose 15g, agar 20g, peptone 8g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml, autoclave;
[0043] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 14 hours, 121°C high-pressure sterilization for 4 hours;
[0044] (2) Activation of strains:
[0045] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;
[0046] Among them, the activated Cordyceps militaris strain is the first generation species;
[0047] The second step: inoculation culture:
[0048]...
Embodiment 2
[0065] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:
[0066] Step 1: Activation of the strains of Cordyceps militaris
[0067] (1) Formula of mother seed medium:
[0068] Potato 200g, glucose 20g, agar 23g, peptone 10g, potassium dihydrogen phosphate 5g, magnesium sulfate 3g, water 1000ml, autoclave;
[0069] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 16 hours, 121°C high-pressure sterilization for 4 hours;
[0070] (2) Activation of strains:
[0071] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;
[0072] Among them, the activated Cordyceps militaris strain is the first generation species;
[0073] The second step: inoculation culture:
[0074...
Embodiment 3
[0091] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:
[0092] Step 1: Activation of the strains of Cordyceps militaris
[0093] (1) Formula of mother seed medium:
[0094] Potato 190g, glucose 18g, agar 22g, peptone 9g, potassium dihydrogen phosphate 3g, magnesium sulfate 2g, water 1000ml, autoclave;
[0095] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 15 hours, 121°C high-pressure sterilization for 4 hours;
[0096] (2) Activation of strains:
[0097] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;
[0098] Among them, the activated Cordyceps militaris strain is the first generation species;
[0099] The second step: inoculation culture:
[0100]...
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