Culture method of cordyceps militaris

A technology of Cordyceps militaris and a cultivation method, applied in the field of cultivating Cordyceps militaris, can solve problems such as unstable yield and quality, and achieve the effects of tidy growth of fruiting bodies, shortened growth cycle, and fast germination.

Inactive Publication Date: 2015-08-12
吴中区胥口精益生物医药研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to propose a method for cultivating Cordyceps militaris in view of the shortcomings of the above prior art. The output, quality instability and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:

[0040] Step 1: Activation of the strains of Cordyceps militaris

[0041] (1) Formula of mother seed medium:

[0042] Potato 180g, glucose 15g, agar 20g, peptone 8g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml, autoclave;

[0043] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 14 hours, 121°C high-pressure sterilization for 4 hours;

[0044] (2) Activation of strains:

[0045] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;

[0046] Among them, the activated Cordyceps militaris strain is the first generation species;

[0047] The second step: inoculation culture:

[0048]...

Embodiment 2

[0065] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:

[0066] Step 1: Activation of the strains of Cordyceps militaris

[0067] (1) Formula of mother seed medium:

[0068] Potato 200g, glucose 20g, agar 23g, peptone 10g, potassium dihydrogen phosphate 5g, magnesium sulfate 3g, water 1000ml, autoclave;

[0069] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 16 hours, 121°C high-pressure sterilization for 4 hours;

[0070] (2) Activation of strains:

[0071] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;

[0072] Among them, the activated Cordyceps militaris strain is the first generation species;

[0073] The second step: inoculation culture:

[0074...

Embodiment 3

[0091] The present embodiment provides a kind of culture method of militaris sinensis, specifically comprises the following steps:

[0092] Step 1: Activation of the strains of Cordyceps militaris

[0093] (1) Formula of mother seed medium:

[0094] Potato 190g, glucose 18g, agar 22g, peptone 9g, potassium dihydrogen phosphate 3g, magnesium sulfate 2g, water 1000ml, autoclave;

[0095] The conditions for high-temperature sterilization are: 100°C normal pressure sterilization for 15 hours, 121°C high-pressure sterilization for 4 hours;

[0096] (2) Activation of strains:

[0097] The day before the activation, put the Cordyceps militaris strains in the refrigerator in a constant temperature incubator at 24°C for 1 day, and transfer the Cordyceps militaris strains to the parent culture medium for activation on the second day;

[0098] Among them, the activated Cordyceps militaris strain is the first generation species;

[0099] The second step: inoculation culture:

[0100]...

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Abstract

The present invention discloses a culture method of cordyceps militaris. The method comprises the following steps: step 1, activating a culture of cordyceps militaris; step 2 carrying out inoculated culture; step 3 carrying out management of producing mushrooms; and step 4, harvesting. The culture method disclosed by the present invention is simple in process and low in cost, and a free room can be used to produce, so that the problems that the output and the quality in artificial culture and production are instable and the like are solved.

Description

technical field [0001] The invention belongs to the technical field of cultivation of medicinal and edible fungi, and in particular relates to a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris belongs to the genus Cordyceps of the family Ergotaceae. It is of the same genus and different species as Cordyceps sinensis. It can grow in middle or shallow mountainous areas and can parasitize on the chrysalis of various insects. Cordyceps militaris has lower requirements on the environment, liquid fermentation can form mycelium, and artificial large-scale solid culture can obtain spores, and its medicinal value is similar to that of the well-known Chinese and foreign Cordyceps sinensis. The components of Cordyceps militaris, such as Cordyceps polysaccharide and cordycepic acid, are equivalent to the content of natural Cordyceps sinensis, and the content of some components even exceeds that of Cordyceps sinensis. For example, the content ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 王肇生王明义
Owner 吴中区胥口精益生物医药研究所
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