Method for growing dendrobium seedlings
A Dendrobium and seed technology, applied in the field of plant cultivation, can solve the problems of poor quality of seedlings, high operating costs, and large investment, and achieve the effects of convenient operation, energy saving, and light reduction
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Embodiment 1
[0019] Dendrobium seedling raising method provided by the invention, through the following steps:
[0020] A. In the inoculation room, inoculate the conventionally sterilized seeds in vitro in the following medium: MS+NAA0.5mg / L+banana puree 50g / L, at a temperature of 22°C, a light intensity of 3000Lx, and a light time of 10 hours per day. Under the condition of cultivating for 60 days, a round bulb will be formed;
[0021] B. Transfer the round corm obtained in step A into the following medium: 1 / 2MS+NAA0.5mg / L+banana mash 50g / L+mashed potato 50g / L, at a temperature of 22°C, a light intensity of 3000Lx, and a light time of 10 Under the condition of 1 hour / day, cultivate for 50 days to form differentiated seedlings;
[0022] C, inoculate the differentiated seedlings gained in the B step in the medium in the seedling bottle, the medium is: 1 / 2MS+agar 4g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available plastic wrap The joint part of the bo...
Embodiment 2
[0024] A. In the inoculation room, inoculate the conventionally sterilized seeds in vitro in the following medium: MS+NAA 2mg / L+banana puree 50g / L, under the conditions of temperature 26°C, light intensity 1500Lx, light time 12 hours a day, Cultivate for 80 days to form a round bulb;
[0025] B. Transfer the round corm obtained in step A into the following culture medium: 1 / 2MS + mashed banana 50g / L + mashed potato 50g / L, under the conditions of 12 hours / day at a temperature of 26°C, light intensity of 1500Lx, and light time, Cultivate for 80 days to form differentiated seedlings;
[0026] C, inoculate the differentiated seedlings of B step in the culture medium in the seedling bottle, the culture medium is: 1 / 2MS+agar 6g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available plastic wrap and The joint part of the bottle body is then placed in an ordinary greenhouse with a shading rate of 80%, and a shading net with a shading rate of 80% is set...
Embodiment 3
[0028] A. In the inoculation room, inoculate the routinely sterilized stem nodes in vitro in the following medium: MS+NAA0.1mg / L+banana puree 50g / L, at a temperature of 24°C, light intensity of 2000Lx, and light time of 11 hours per day. Under the condition of culturing for 60 days, a round bulb will be formed;
[0029] B. Transfer the round corm obtained in step A into the following medium: 1 / 2MS+NAA0.2mg / L+banana mash 50g / L+mashed potato 50g / L, at a temperature of 24°C, a light intensity of 2000Lx, and a light time of 11 Under the condition of hour / day, cultivate for 65 days to form differentiated seedlings;
[0030] C, inoculate the differentiated seedlings of B step in the culture medium in the seedling bottle, the culture medium is: 1 / 2MS+agar 5g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available preservative film and The joint part of the bottle body is then placed in an ordinary greenhouse with a shading rate of 70%, and a shade net ...
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