Method for growing dendrobium seedlings

A Dendrobium and seed technology, applied in the field of plant cultivation, can solve the problems of poor quality of seedlings, high operating costs, and large investment, and achieve the effects of convenient operation, energy saving, and light reduction

Inactive Publication Date: 2011-08-10
红河群鑫石斛种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of large investment, high energy consumption, high operating costs, difficult management, and poor quality of seedlings in the prior art, the invention provides a method for raising dendrobium seedlings with low consumption, easy management and excellent seedling quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dendrobium seedling raising method provided by the invention, through the following steps:

[0020] A. In the inoculation room, inoculate the conventionally sterilized seeds in vitro in the following medium: MS+NAA0.5mg / L+banana puree 50g / L, at a temperature of 22°C, a light intensity of 3000Lx, and a light time of 10 hours per day. Under the condition of cultivating for 60 days, a round bulb will be formed;

[0021] B. Transfer the round corm obtained in step A into the following medium: 1 / 2MS+NAA0.5mg / L+banana mash 50g / L+mashed potato 50g / L, at a temperature of 22°C, a light intensity of 3000Lx, and a light time of 10 Under the condition of 1 hour / day, cultivate for 50 days to form differentiated seedlings;

[0022] C, inoculate the differentiated seedlings gained in the B step in the medium in the seedling bottle, the medium is: 1 / 2MS+agar 4g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available plastic wrap The joint part of the bo...

Embodiment 2

[0024] A. In the inoculation room, inoculate the conventionally sterilized seeds in vitro in the following medium: MS+NAA 2mg / L+banana puree 50g / L, under the conditions of temperature 26°C, light intensity 1500Lx, light time 12 hours a day, Cultivate for 80 days to form a round bulb;

[0025] B. Transfer the round corm obtained in step A into the following culture medium: 1 / 2MS + mashed banana 50g / L + mashed potato 50g / L, under the conditions of 12 hours / day at a temperature of 26°C, light intensity of 1500Lx, and light time, Cultivate for 80 days to form differentiated seedlings;

[0026] C, inoculate the differentiated seedlings of B step in the culture medium in the seedling bottle, the culture medium is: 1 / 2MS+agar 6g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available plastic wrap and The joint part of the bottle body is then placed in an ordinary greenhouse with a shading rate of 80%, and a shading net with a shading rate of 80% is set...

Embodiment 3

[0028] A. In the inoculation room, inoculate the routinely sterilized stem nodes in vitro in the following medium: MS+NAA0.1mg / L+banana puree 50g / L, at a temperature of 24°C, light intensity of 2000Lx, and light time of 11 hours per day. Under the condition of culturing for 60 days, a round bulb will be formed;

[0029] B. Transfer the round corm obtained in step A into the following medium: 1 / 2MS+NAA0.2mg / L+banana mash 50g / L+mashed potato 50g / L, at a temperature of 24°C, a light intensity of 2000Lx, and a light time of 11 Under the condition of hour / day, cultivate for 65 days to form differentiated seedlings;

[0030] C, inoculate the differentiated seedlings of B step in the culture medium in the seedling bottle, the culture medium is: 1 / 2MS+agar 5g / L+sucrose 20g / L, cover the bottle cap, seal the bottle cap with commercially available preservative film and The joint part of the bottle body is then placed in an ordinary greenhouse with a shading rate of 70%, and a shade net ...

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Abstract

The invention provides a method for growing dendrobium seedlings, which includes in vitro inoculation of seeds, formation of circular corms through cultivation, and formation of differentiation seedlings by the cultivation of the circular corms, and is characterized by steps as follows: inoculating the differentiation seedlings on a 1 / 2 MS culture medium in a seedling raising bottle; covering with a lid; sealing the part of the lid which is connected with the bottle body with a fresh-keeping film; then placing the bottle in an ordinary greenhouse with a shading rate of 60-80% and after growing in a natural lighting environment with a temperature of 5-34 DEG and illumination intensity of 3500-9000Lx for 2 months, taking the seedlings out of the bottle and permanent-planting in a field. Theinvention can save not only the electric energy consumed in the commodity seedling culturing phase, but also the cost incurred by sterilization, sterilization medicine and manpower, and is convenientto be operated, time-saving and labor-saving. Particularly, after the culturing in the natural lighting environment, the antibacterial and disease-resistant capability of the commodity seedlings is improved, and the planting survival rate of dendrobium seedlings is greatly increased and attains over 90%.

Description

technical field [0001] The invention relates to a method for raising seedlings, in particular to a method for raising seedlings of dendrobium officinale and dendrobium dendrobium, belonging to the technical field of plant cultivation. Background technique [0002] Dendrobium is a traditional precious Chinese medicine. There are more than 70 varieties such as Tiepi, Nobile, Huoshan, Huancao, and Horsewhip. Among them, Dendrobium candidum can be used to treat fever due to its functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, moistening lung and relieving cough. Injured body fluid, dry mouth and polydipsia, asthenia heat after illness and other diseases. In addition, modern pharmacological studies have shown that the polysaccharide contained in Dendrobium candidum has anti-tumor, anti-aging, enhancing human immunity and dilating blood vessels, etc., and its medicinal value is extremely high. Therefore, Dendrobium candidumWal1.ex Lind1 i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 查应洪
Owner 红河群鑫石斛种植有限公司
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