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65 results about "Natural processes" patented technology

Isothermal amplification method for rapid detection of listeria monocytogenes

The invention provides a real-time fluorescent quantitative helicase-dependent isothermal nucleic acid amplification method for detecting listeria monocytogenes. Primer pair sequences having specific amplification function on listeria monocytogenes are designed so as to reflect a natural process for simulating the replication of in-vivo DNA at a constant temperature, DNA double strands are solved at a constant temperature by virtue of helicase, the listeria monocytogenes genomic DNA is adopted as a template, specific isothermal nucleic acid amplification is performed so as to achieve the exponential growth of the target sequence, the real-time fluorescent helicase-dependent isothermal nucleic acid amplification detection method is established, detection results are analyzed by virtue of an isothermal amplification curve and melting curve of the genomic DNA so that listeria monocytogenes is rapidly detected. By the method, the disadvantage that the traditional PCR needs to rely on the repeated heating and cooling of an instrument to obtain the single-stranded template is overcome and the method is simple in principle, convenient to operate and listeria monocytogenes in foods can be rapidly, simply, sensitively and specifically detected.
Owner:JIANGNAN UNIV

Preparation method of wolfberry cooking wine

The invention relates to a preparation method of wolfberry cooking wine, and especially relates to a preparation method of wolfberry cooking wine with the alcohol degree of 18-25% (vol) and based on brewed liquor or yellow rice wine. The preparation method of the wolfberry cooking wine comprises the following steps: preprocessing a main raw material wolfberry by adopting an ultralow temperature refrigeration sublimation technology to prepare freeze-dried wolfberry whole powder, extracting, and carrying out fine filtration. Main ingredients comprise hawthorn fruit and dried orange peel. The wolfberry cooking wine has pure color and clarified body, and has the mellow aroma of natural wolfberry. Each 100mL of the cooking wine contains 10-20g of natural wolfberry nutritional components. The method is mainly characterized in that base wine used in the immersion process is brewed liquor or yellow rice wine, and the raw material wolfberry is completely integrated and coordinated to other plant components in natural process state to naturally complete the preparation. The wolfberry cooking wine has the unique mellowness of wolfberry, the preparation method is a brand new cooking wine preparation method, and the wolfberry cooking wine makes high-end cooking and characteristic dishes better, and is a high-quality nutritional cooking wine.
Owner:宋庆元

Cardamine hupingshanesis water-soluble activated selenium polypeptide extract and extraction method thereof

The invention discloses a cardamine hupingshanesis water-soluble activated selenium polypeptide extract. The extract is obtained in the following steps that cardamine hupingshanesis is smashed and subjected to water extraction to obtain a water extract, the water extract is filtered by means of a filter screen, residues are removed to obtain filtrate, the filtrate is pressurized and filtered by a filter membrane to obtain microfiltration liquid, the microfiltration liquid is pressurized and filtered by an ultrafiltration membrane with the molecular weight being 1000-3000 Dalton to obtain ultrafiltration liquid, the ultrafiltration liquid is pressurized and filtered by a nanofiltration membrane with the molecular weight being 150 Dalton to remove inorganic salt and water to obtain a nanofiltration concentrated solution, ethyl alcohol is added into the nanofiltration concentrated solution, and the mixture stands still and is filtered to obtain precipitate, namely the cardamine hupingshanesis water-soluble activated selenium polypeptide extract. Large-molecular impurities like pectin, polysaccharide and starch are effectively removed sequentially through microfiltration, ultrafiltration and nanofiltration, and high-purity cardamine hupingshanesis water-soluble activated selenium polypeptide with the molecular weight being 150-3000 Dalton is obtained. The method is a pure physical natural process and is suitable for large-scale production and extraction of cardamine hupingshanesis water-soluble activated selenium polypeptide.
Owner:ANKANG UNIV
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