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Cardamine hupingshanesis water-soluble activated selenium polypeptide extract and extraction method thereof

An extraction method and technology of saxifrage, applied in the field of water-soluble plant active selenium polypeptide extract of saxifrage and its extraction, can solve the problems of large loss, large product molecular weight, no extraction and purification method, etc., to avoid protein Denatured, low-purity effect

Inactive Publication Date: 2016-12-07
ANKANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of the plant selenoprotein currently requires degreasing, enzymatic hydrolysis, degumming and multiple alcohol precipitation steps. The operation is complicated, the loss is large, and the molecular weight of the obtained product is relatively large. So far, no water-soluble Low molecular weight active selenium polypeptide and its extraction and purification method and preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The extraction method of the water-soluble plant active selenium polypeptide extract of saxifrage comprises the following steps:

[0025] (1) Pulverize the tiger ear gold;

[0026] (2) Add saxiferium (broken rice chestnut) powder into water at a ratio of 1:10 (mass ratio), extract it with ultrasound at 30-60°C, and use the enzymes present in the plant to hydrolyze the large molecular weight selenium Protein, remove inorganic selenium. Ultrasonic water extraction for 3 times, 30-40min each time, combined water extract;

[0027] (3) filter the water extract to remove the residue and obtain the filtrate;

[0028] (4) Filtrate the obtained saxifolium (broken rice chestnut in a pot and bottle) through pressure filtration with a 0.2 μm filter membrane to obtain a microfiltrate;

[0029] (5) The microfiltrate is pressure-filtered with a 1000-3000 Dalton ultrafiltration membrane to obtain an ultrafiltrate; the purpose of this step is to remove water-soluble macromolecular su...

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Abstract

The invention discloses a cardamine hupingshanesis water-soluble activated selenium polypeptide extract. The extract is obtained in the following steps that cardamine hupingshanesis is smashed and subjected to water extraction to obtain a water extract, the water extract is filtered by means of a filter screen, residues are removed to obtain filtrate, the filtrate is pressurized and filtered by a filter membrane to obtain microfiltration liquid, the microfiltration liquid is pressurized and filtered by an ultrafiltration membrane with the molecular weight being 1000-3000 Dalton to obtain ultrafiltration liquid, the ultrafiltration liquid is pressurized and filtered by a nanofiltration membrane with the molecular weight being 150 Dalton to remove inorganic salt and water to obtain a nanofiltration concentrated solution, ethyl alcohol is added into the nanofiltration concentrated solution, and the mixture stands still and is filtered to obtain precipitate, namely the cardamine hupingshanesis water-soluble activated selenium polypeptide extract. Large-molecular impurities like pectin, polysaccharide and starch are effectively removed sequentially through microfiltration, ultrafiltration and nanofiltration, and high-purity cardamine hupingshanesis water-soluble activated selenium polypeptide with the molecular weight being 150-3000 Dalton is obtained. The method is a pure physical natural process and is suitable for large-scale production and extraction of cardamine hupingshanesis water-soluble activated selenium polypeptide.

Description

technical field [0001] The invention relates to a saxifrage water-soluble plant active selenium polypeptide extract and an extraction method thereof. Background technique [0002] Selenium plays an important role in human health. Selenoproteins have the advantages of low toxicity and high bioavailability. Among them, animal selenoproteins are superior to plant selenoproteins, plant selenoproteins are superior to microbial selenoproteins, and microbial selenoproteins are superior to inorganic selenium. The main ingredient of selenium yeast is selenomethionine, and plant organic selenium is better than selenocysteine. In particular, "peptides" with a molecular weight of 3000-1000 and pure natural small-molecule active selenium polypeptides with a molecular weight of 1000-180 can be directly absorbed by the digestive system, and have higher bioavailability than ordinary selenoproteins with larger molecular weights. It is one of the frontiers of organic selenium research at h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/14
CPCC07K1/145C07K1/30C07K1/34C07K1/36
Inventor 谢娟平
Owner ANKANG UNIV
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