Cuttage propagation method for survived seedlings by tissue culture and transplantation of catalpa bungei

A technique of tissue culture and cutting propagation, applied in botany equipment and methods, cultivation, soilless cultivation, etc., to achieve the effects of reducing production costs, increasing promotion speed and scale, and high survival rate

Inactive Publication Date: 2010-06-09
LIANYUNGANG FORESTRY TECHN GUIDANCE STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report yet for the method of utilizing catalpa tissue culture to be transplanted into living seedlings for cutting propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In spring, summer and autumn, when "Wanchu No. 1" is transplanted into live seedlings and grows to 50cm, cut the upper 30cm long branches, and then cut them to about 5cm long as cuttings, and the lower cut is smooth and inclined at 45°. The upper incision is smooth and flat, about 1cm away from the bud, leaving 1 leaf at the top of the cuttings, cutting off 2 / 3 of the remaining leaves, and keeping 1 / 3; dip the base of the cut cuttings in the prepared root-stimulating agent for 8 seconds. (The root promoting agent uses indole butyric acid (IBA) 200mg, pyridoxine hydrochloride (VB 6 )3mg and sodium molybdate (Na 2 MoO 4 2H 2 O) 0.5mg and 1kg of water are prepared to make a mixed solution). The cuttings cuttages treated with the above-mentioned root-promoting agent are placed in a plug tray equipped with a substrate, and the substrate is prepared by mixing perlite and peat in a ratio of 5:2, and using 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution S...

Embodiment 2

[0017] In spring, summer, and autumn, when the live seedlings transplanted by tissue culture of "Yuchu No. 1" grow to 80cm, cut the upper 45cm long branches, and then cut them to about 15cm long as cuttings. The lower cut is smooth and inclined at 45°. The upper incision is smooth and flat, about 1cm away from the buds, leaving 1 leaf on the top of the cuttings, cutting off 2 / 3 of the remaining leaves, and keeping 1 / 3; dip the base of the cut cuttings in the prepared root-stimulating agent for 5 seconds. (The root promoting agent uses indole butyric acid (IBA) 500mg, pyridoxine hydrochloride (VB 6 ) 8mg and sodium molybdate (Na 2 MoO 4 2H 2 O) Add 1.0 mg of water to 1 kg to form a mixed solution). The cuttings cuttings treated with the above-mentioned root-promoting agent are placed in a plug tray equipped with a substrate, and the substrate is prepared by mixing perlite and peat in a ratio of 5:5, and using 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution...

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Abstract

The invention discloses a cuttage propagation method for survived seedlings by tissue culture and transplantation of catalpa bungei, and is characterized by comprising the following steps of: 1), preparing a cuttage substrate and a root growth promoting agent; 2) scissoring 30 to 50cm of twigs when the survived seedlings obtained through the tissue culture and transplantation of the selected good singleplant tissue grow to a height between 50 and 80cm, shearing the twigs into 5 to 15cm of small segments and reserving 1/3 blades, dipping the small segments into the prepared root growth promoting agent for 5 to 8 s, and inserting the dipped small segments into the prepared substrate; and 3) inserting cuttingwood into a potted tray containing the substrate, then totally sealing the potted tray with a plastic film, and keeping the relative humidity between 85 and 95 percent, and the temperature between 20 and 30 DEG C, wherein a cuttingwood base starts generating callus after 10 to 13 days, the callus grows a root system after 18 to 21 days, the root system grows buds after 25 to 30 days, and the seedlings are transplanted after 40 days. The method can keep the original good properties of a catalpa bungei stock plant, and has over 95 percent of cuttage survival rate, low cost and high efficiency.

Description

technical field [0001] The invention relates to a cutting propagation method of plants, in particular to a cutting propagation method of excellent single catalpa tree tissue culture and transplanting into living seedlings, belonging to the technical field of asexual propagation of forest seedlings. Background technique [0002] Catalpa bungei C.A. Meyer. belongs to the Bignoniaceae family and belongs to the genus Catalpa. It has been cultivated in my country for more than 2,000 years. It is a high-quality timber tree species traditionally cultivated in my country. It is famous for its excellent material and is known as the "King of Wood". . Catalpa has a straight trunk and a graceful crown, with a height of more than 30m and a diameter at breast height of more than 1m. The branches and leaves of catalpa are luxuriant, and the flowering period is from April to May. The flowers are colorful and lovely. People are pleasing to the eye. The fruit ripening period is from Septemb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00C05G1/00C05G3/00
Inventor 王定胜张永忠王永昌蒋泽平黄建庭
Owner LIANYUNGANG FORESTRY TECHN GUIDANCE STATION
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