Tissue culture and rhizome expansion method of red torch turmeric

Abstract

The invention discloses a method for tissue culture and rhizome expansion of turmeric turmeric. The steps include 1) germination acceleration of rhizomes; 2) preparation of explants; 3) aseptic seedling cultivation: the medium is MS; at a temperature of 25±2°C , light intensity 1500Lx, and light time 12h / d under tissue culture conditions for 7‑10d; 4) Adventitious bud induction culture: the medium is MS+6‑BA3.0mg / L+TDZ 0.2 mg / L; the temperature is 25± 2°C, the light intensity is 1500Lx, and the light time is 12h / d; 5) Proliferation culture and rhizome expansion: the medium is: MS+6‑BA 2.0 mg / L+NAA 0.3mg / L, and methyl jasmonate 2.0 mg / L: 6) rooting culture. The invention has a rooting rate of 100%, shortens the multiplication and rooting period, and makes the seedling production of "red torch" turmeric not limited by time and season.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture rapid propagation and rhizome expansion of "Red Torch" turmeric. Background technique [0002] "Red Torch" tulips ( Curcuma hybrida 'Red Torch') is a perennial herbaceous bulbous flower of the genus Curcuma in the Zingiberaceae family. It has spikes, pedicels higher than the leaves, many bracts, beautiful and bright colors, and a long-lasting flowering period. It can not only enrich high-grade cut flower varieties in my country, but also Using it as a potted flower for ornamental purposes or for landscaping has high ornamental value, good quality, high economic benefits, and broad prospects for development and utilization. But at present, only conventional bulbs can be used for vegetative propagation, the propagation speed is slow and the quality of the bulbs declines, which cannot meet the market demand. High-quality and neat seedlings c...

AI Technical Summary

Problems solved by technology

"Study on detoxification tissue culture technology of Wenyujin" (Wang Hong et al., Pharmaceutical Biotechnology, 2009, 6), "Cultivation and rapid propagation of Wenyujin callus" (Wang Xiaohui, etc., Northern Horticulture, 2008, 10), "Wenyujin tissue culture Cultivation and Rapid Propagation" (Wang Hong et al., Plant Physiology Communication, 2007, 3) and "Methods for Detoxification and Rapid Propagation of Curcuma Curcuma" (Patent Application No.: 200710070702.7), although a tissue culture rapid propagation method for Curcuma curcuma has been reported, but , the related methods also have the following deficiencies: 1) Since the terminal buds or axillary buds grown on underground rhizomes are mostly used as explants for tissue culture, it is difficult to sterilize and sterilize, and the contamination rate of tissue culture is high; 2) Adventitious buds 3-5 adventitious buds can be differentiated from each explant; it takes 80-90 days from adventitious bud induction to proliferation and rooting, the production cycle is longer and the efficiency becomes lower; 3) The produced tissue culture seedlings The rhizomes are relatively slender, and the "Red Torch" turmeric needs the relevant nutrients accumulated in the rhizomes to promote growth during the growth process. The seedlings with smaller rhizomes have a lower survival rate after transplanting, generally at 90%, and the quality of the seedlings decreases. The growth is slow, which leads to the failure of normal flowering in the current year, and the harvested bulbs need to be planted in the second or even third year before they can bloom