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Method for measuring malonaldehyde content of catalpa bungei leaves

A technology of sheet malondialdehyde and determination method, which is applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of malondialdehyde content. detection, etc.

Inactive Publication Date: 2019-05-21
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that malondialdehyde (MDA) content cannot be detected in catalpa leaf, the purpose of the invention is to provide a kind of method that accurately detects malondialdehyde (MDA) content in catalpa leaf

Method used

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  • Method for measuring malonaldehyde content of catalpa bungei leaves
  • Method for measuring malonaldehyde content of catalpa bungei leaves
  • Method for measuring malonaldehyde content of catalpa bungei leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The detection effect of malondialdehyde content of catalpa leaf under the different concentration of iron sulfate of embodiment 1

[0036] (1) Material

[0037] The young leaves of 1-year-old catalpa seedlings were used as materials.

[0038] (2) Reagent preparation

[0039] Trichloroacetic acid (TCA) solution: configure the concentration of trichloroacetic acid solution to be 0.75%.

[0040] Thiobarbituric acid (TBA) solution: 0.02mol / L.

[0041] Iron Sulfate (Fe 2 (SO 4 ) 3 ) solution: 0.01g / L and 0.02g / L.

[0042] (3) Measurement process

[0043] Grind the leaf sample into powder with liquid nitrogen, weigh 4.50g (accurate to 0.01g) of the sample powder, place it in a stoppered Erlenmeyer flask, add 45mL TCA solution accurately, shake well and stopper, place it on a constant temperature shaker at 50°C 30min, the rotation frequency is 110rpm, take it out, filter with filter paper, and take the filtrate for later use.

[0044] Take 9 extracts respectively and ...

Embodiment 2 3

[0047] The detection effect of catalpa leaf malondialdehyde content under the different concentrations of embodiment 2 trichloroacetic acid

[0048] (1) Material

[0049] The young leaves of three 1-year-old catalpa grafted seedlings were used as materials, and they were numbered as sample 1, sample 2 and sample 3 respectively.

[0050] (2) Reagent preparation

[0051]Trichloroacetic acid (TCA) solution: prepare the concentration of trichloroacetic acid solution as 0.5% and 1.0%.

[0052] Thiobarbituric acid (TBA) solution: 0.02mol / L.

[0053] Iron Sulfate (Fe 2 (SO 4 ) 3 ) solution: 0.02g / L.

[0054] (3) Measurement process

[0055] First, according to the design of 3 repetitions of 2 concentrations of TCA solutions, accurately weigh 6 parts of sample 1, sample 2 and sample 3, each 1.00g, put them into a mortar, add 2ml of 0.5% and 1.0 % TCA, grind until homogenized, transfer the homogenate to a 25ml centrifuge tube, and wash it with 8ml of TCA with a concentration of...

Embodiment 3

[0060] The influence of embodiment 3 different temperatures on MDA extraction effect

[0061] (1) Material

[0062] The leaves of 1-year-old catalpa seedlings were used as materials.

[0063] (2) Reagent preparation

[0064] Trichloroacetic acid (TCA) solution: the concentration is 0.75%.

[0065] Thiobarbituric acid (TBA) solution: the concentration is 0.02mol / L.

[0066] Iron Sulfate (Fe 2 (SO 4 ) 3 ) solution: the concentration is 0.02g / L.

[0067] (3) Measurement process

[0068] First, according to the design of 3 repetitions at 3 temperatures, 9 samples were accurately weighed, each 1.00g, put into a mortar, 2ml of TCA with a concentration of 0.75% was added, ground to a homogenate, and the homogenate was transferred to 25ml centrifuge tube, and rinsed with 8ml of TCA with a concentration of 0.75%; then, shake the centrifuge tube in a constant temperature shaker at 50°C, 60°C, and 70°C for 30min at a rotation frequency of 110rpm; finally, take out the shaken cent...

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Abstract

The invention relates to a method for measuring the malonaldehyde content of catalpa bungei leaves. The method comprises: firstly, carrying out ice bath grinding on collected fresh leaves; then addingtrichloroacetic acid and shaking in a constant temperature shaker to extract malondialdehyde; after an extracting solution is acquired, adding ferric sulfate and a thiobarbituric acid to eliminate soluble sugar interference and carrying out chromogenic reaction; and finally, using a spectrophotometer or a microplate reader to detect the light absorption values of the reaction solution at 450 nm,532 nm, and 600 nm wave lengths, and calculating the malonaldehyde content in the leaves. By using the method for measuring the malonaldehyde content of the catalpa bungei leaves, a problem that themalonaldehyde content of the catalpa bungei leaves can not be rapidly detected is solved; the method is suitable for large scale and simple operation; technical guarantee is provided for the measurement of the malonaldehyde content of the catalpa bungei leaves; and the method is suitable for the evaluation of catalpa bungei germplasm resources, the selection of diverse and appropriate new varieties, the development of a cultivation technology, the measurement of a plant damage degree and other aspects.

Description

technical field [0001] The invention relates to a method for detecting secondary metabolites in plant tissues, in particular to a method for determining the content of malondialdehyde in catalpa leaves. Background technique [0002] Malondialdehyde (MDA) is the final decomposition product of membrane lipid peroxidation in plants under stress or aging. After MDA is released from the place where it is produced on the membrane, it can react with proteins and nucleic acids to change the properties of these macromolecules. configuration, or make it produce cross-linking reaction, thereby losing its function, and also relax the bridge bonds between cellulose molecules, or inhibit protein synthesis. Therefore, the accumulation of MDA will cause certain damage to membranes and cells, and its content can be Reflects the extent to which plants suffer adversity damage. [0003] Under acidic and high temperature conditions, MDA can react with thiobarbituric acid (TBA) to form a reddish...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 麻文俊王军辉刘莹杨桂娟何润华易飞
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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