Rooting culture method of catalpa bungei tissue culture seedling

Abstract

The invention discloses a rooting culture method of catalpa bungei tissue culture seedling. The method sequentially comprises the following steps: (1) obtaining aseptic seedlings of the catalpa bungei; (2) subculturing the aseptic seedlings; (3) performing rooting culture of the catalpa bungei tissue culture seedlings; (4) hardening seedlings and transplanting. By adopting the catalpa bungei tissue culture seedling rooting culture method, the propagation ratio is controlled to 1: (3-3.5), the height of the plant of the seedlings is controlled to be 2.5-4cm, and the rooting culture is carried out to guarantee that the rooting proportion is up to 90 percent or above; the production cost is reduced, and the same culture medium is used in the steps of obtaining the aseptic seedlings and subculturing during the rooting culture of the catalpa bungei tissue culture seedling, so that an operation step is eliminated and the production cost is reduced, thus being more suitable for industrial production. According to the rooting culture method of the catalpa bungei tissue culture seedling, the forest tissue culture industrialization for an experiment is realized, the industrialization process is simplified, the steps are reduced, the labor force is controlled, and the cost control is facilitated, thus promoting the industrialization of the woody plant tissue culture. The method is suitable for the rooting culture of the catalpa bungei tissue culture seedling.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for plant tissue culture, in particular to a method for rooting culture of catalpa tree tissue culture seedlings. Background technique [0002] Catalpa bungei C.A.Meyer, belonging to Bignoniaceae and Catalpa genus, is a deciduous tree. Under good site conditions, the height of the tree can reach 30m, and the diameter at breast height can reach more than 1m. However, in terms of reproduction, since the male and female flowering periods of Catalpa do not meet, they are often self-sterile and require artificial pollination to bear fruit. The rate of seed production is very low, the rate of seed germination is not high, and seed reproduction is relatively difficult, resulting in relatively few resources of Catalpa. At present, the root tiller or grafting methods are mostly used to produce catalpa seedlings. With the increase of felling and utilization of catalpa, the conventi...

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Problems solved by technology

[0006] In the above studies on the proliferation medium of catalpa, the hormone ratio is mainly based on the combination of cytokinin and auxin, and the research is mostly at the theoretical level, and the rooted seedlings of tissue culture need to be hardened before transplanting in the greenhouse. In the industrial production process, this step consumes a lot of labor costs, and reducing the cost of hardening seedlings is another difficult problem to be solved in the industrial production of catalpa tissue culture

[0007] Chinese invention patent application CN104054580 A discloses "a kind of tissue culture method that improves catalpa multiplication coefficient", comprises the following steps (1) establishment of sterile culture; (2) induction of axillary buds; (3) proliferation of axillary buds; ( 4) rooting culture of sprouts; (5) hardening and transplanting, five steps, the best axillary bud induction formula for this patent application is MS+BA 0.8mg / L+IBA 0.1mg / L; the best axillary bud proliferation formula is MS+BA1.5mg / L+ZT O.1mg / L+IAA 0.2mg / L; the highest multiplication coefficient is 15, although this method obtains a higher multiplication coefficient, but the multiplication is mainly realized through two steps of axillary bud induction and axillary bud multiplication , if factory production is carried out, the method steps are cumbersome and the labor cost is large, which is not conducive to large-scale factory production

[0008] In the industrial production process of catalpa, it is necessary not only to ensure the expansion ratio, but also to ensure the ratio of seedlings that can be used for rooting. However, most of the existing documents and reports are limited to the theoretical level. The rooting materials need to be selected from the expansion seedlings, which can be used for rooting. The proportion of seedlings is extremely low, which is difficult to meet the requirements of industrial production

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