Method for generating somatic embryos of catalpa bungei through induction

A technology of somatic embryos and catalpa, applied in the field of bioengineering

Inactive Publication Date: 2014-09-17
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the induction of somatic embryos in Catalpa

Method used

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  • Method for generating somatic embryos of catalpa bungei through induction
  • Method for generating somatic embryos of catalpa bungei through induction
  • Method for generating somatic embryos of catalpa bungei through induction

Examples

Experimental program
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Embodiment Construction

[0018] (1) Treatment of mature catalpa seeds

[0019] Wrap the mature catalpa seeds with gauze and rinse with tap water for 30 minutes, then sterilize the surface with 75% alcohol for 60 seconds, rinse with sterile water twice, then sterilize with 0.1% mercuric chloride solution for 12 minutes, and rinse with sterile water for 6 times. When inoculating, cut the gauze, and slightly cut the seed coats at the edges of the seeds for inoculation. When inoculating, cut the gauze, and slightly cut the seed coats at the edges of the seeds for inoculation. 3 seeds were received in each bottle, 30 bottles were treated, and repeated 3 times.

[0020] (2) Callus induction

[0021] Spread the processed catalpa seeds on the callus-inducing medium of 1 / 2MS+0.3-0.5mg L zeatin+0.3-0.9mg L indole acetic acid, add 3% sucrose to the medium, and adjust the pH= 5.8. Place the culture medium in a culture room at a temperature of 25-28°C without light and culture in the dark for 20 days to obtain ...

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PUM

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Abstract

The invention provides a method for generating somatic embryos of catalpa bungei through induction. The method comprises the steps of after matured catalpa bungei seeds are disinfected, putting the catalpa bungei seeds into a culture medium for callus induction, generating callus tissues through dark cultivation, then performing subculture multiplication cultivation on the callus tissues to obtain a large amount of calluses, and finally paving the calluses into an induction culture medium to generate the somatic embryos of the catalpa bungei through the induction. A mature regeneration system for cultivating a new insect and disease-resistant product of the catalpa bungei by a transgenic technology is supplied.

Description

technical field [0001] The invention relates to a method of bioengineering technology, in particular to a plant tissue culture technology, in particular to the induction of somatic embryos of Chinese catalpa. Background technique [0002] Catalpa bungei is a tall tree of the Bignoniaceae genus Catalpa. It is native to China and has a cultivation history of more than 2,000 years. The catalpa tree is tall and straight, with a dense crown. The branches, leaves and flowers all have high ornamental value. Among China's rich tree resources, only its "wood" has both appearance and appearance, and has been known as the "King of Wood" since ancient times. Catalpa has strong adaptability. It is distributed in the vast area from the coast in the east to Gansu in the west, Yunnan in the south, and the Great Wall in the north. It is an excellent and precious timber tree species that can be cultivated in large areas. Catalpa tree pests and diseases have been serious in recent years, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王鹏杨如同李亚马玲玲汪庆李林芳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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