Tissue culture method for increasing differentiation number of effective new shoots of large-leaf catalpa bungei

Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture method for increasing the differentiation number of effective new shoots of large-leaf catalpa bungei. According to the method, a set of combined culture mediums is utilized for increasing the differentiation number of the effective new shoots of large-leaf catalpa bungei, wherein thecombined culture mediums include the induction culture medium, the differentiation culture medium and the growth culture medium. The tissue culture method for increasing the differentiation number ofthe effective new shoots of large-leaf catalpa bungei comprises the steps of establishment of a sterile system, subculture in a two-step relay mode, rooting induction culture, seedling hardening, transplanting and the like. By means of the tissue culture method for large-leaf catalpa bungei, on one hand, the components of the culture mediums are optimized and adjusted; on the other hand, the culture mode is adjusted in the subculture process, tissue culture seedlings are subjected to subculture in the specific culture medium in the two-step relay mode, the total culture time is not prolonged,the proliferation and growth process is considered at the same time, the number of the effective new shoots is increased, and meanwhile the vitrification phenomenon in the tissue culture process is well avoided.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method for increasing the number of effective new shoot differentiation of Phrysus japonicus. Background technique [0002] Catalpa ( Catalpa bungi C.A.Mey) is one of the relatively ancient tree species in my country. It has a series of advantages, mainly including: straight trunk, beautiful tree shape, pink flower color; strong noise reduction and dust retention capabilities, and strong resistance to harmful gases Absorption; its self-pollination is poor, and there is no fruit drop to pollute the environment. At the same time, the leaves are concentrated and easy to clean; the material is excellent, and the processing and application fields are extensive. Golden chrysalis ( Catalpa bungi var. ) is a geographical variety of Catalpa, and the big-leaved Catalpa is an excellent clone selected from the seedling population of Catalpa. Compar...

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Problems solved by technology

And with regard to the research on the tissue culture technology of Chinese catalpa, the existing technology of adopting single culture medium to carry out the multiplication of Chinese catalpa (Zhai Xiaoqiao etc., research on the tissue culture and plant regeneration technology of the stem section of Chinese catalpa, Journal of Henan Agricultural University, 2002, 36 (4): 319-321, the culture medium is WPM+IBA0.3 mg / L+6-BA 5 mg / L) After research, it was found that due to the high concentration of 6-BA (5 mg / L), when this method is used for tissue culture and rapid propagation of Catalpa chinensis, as the number of subcultures increases, the tissue culture seedlings are prone to problems such as large callus, short seedlings, vitrification, etc., resulting in unstable proliferation and can be The number of effective shoot differentiations for subculture or rooting culture is low (see attached figure 2 shown), it is difficult to meet the production requirements of tissue culture industrial seedlings

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