A tissue culture method for increasing the number of effective new shoot differentiation of Phrysus japonicus

Abstract

The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method for increasing the number of effective new shoot differentiations of P. chinensis. The method utilizes a group of combination culture medium to improve the differentiation quantity of new shoots of Phryrocystus macrophyllum, and the combination culture medium includes an induction medium, a differentiation medium and a growth medium. The tissue culture method for improving the number of differentiation of new shoots of the golden chinensis comprises: establishment of an aseptic system, subculture in a two-step relay mode, induced rooting culture, hardening of seedlings, transplanting and the like. The tissue culture method of Golden Siberia japonicus provided by this application, on the one hand, optimizes and adjusts the components of the medium, and on the other hand, adjusts the culture method in the process of subculture, and adopts a two-step relay method to carry out tissue culture seedlings on a specific medium. Subculture, while not prolonging the overall culture time, takes into account the process of proliferation and growth, increases the number of effective new shoots, and at the same time better avoids the occurrence of vitrification during the tissue culture process.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method for increasing the number of effective new shoot differentiation of Phrysus japonicus. Background technique [0002] Catalpa ( Catalpa bungi C.A.Mey) is one of the relatively ancient tree species in my country. It has a series of advantages, mainly including: straight trunk, beautiful tree shape, pink flower color; strong noise reduction and dust retention capabilities, and strong resistance to harmful gases Absorption; its self-pollination is poor, and there is no fruit drop to pollute the environment. At the same time, the leaves are concentrated and easy to clean; the material is excellent, and the processing and application fields are extensive. Golden chrysalis ( Catalpa bungi var. ) is a geographical variety of Catalpa, and the big-leaved Catalpa is an excellent clone selected from the seedling population of Catalpa. Compar...

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Problems solved by technology

And with regard to the research on the tissue culture technology of Chinese catalpa, the existing technology of adopting single culture medium to carry out the multiplication of Chinese catalpa (Zhai Xiaoqiao etc., research on the tissue culture and plant regeneration technology of the stem section of Chinese catalpa, Journal of Henan Agricultural University, 2002, 36 (4): 319-321, the culture medium is WPM+IBA0.3 mg / L+6-BA 5 mg / L) After research, it was found that due to the high concentration of 6-BA (5 mg / L), when this method is used for tissue culture and rapid propagation of Catalpa chinensis, as the number of subcultures increases, the tissue culture seedlings are prone to problems such as large callus, short seedlings, vitrification, etc., resulting in unstable proliferation and can be The number of effective shoot differentiations for subculture or rooting culture is low (see attached figure 2 shown), it is difficult to meet the production requirements of tissue culture industrial seedlings

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