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Method for regenerating plants by high-frequency induction of leaves of catalpa bungei

A regeneration plant, high-frequency induction technology, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low induction rate, limited differentiation efficiency, and lack of tissue culture technology

Active Publication Date: 2020-06-02
LUDONG UNIVERSITY
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Problems solved by technology

At present, the tissue culture technology for Chinese catalpa is very scarce, and the existing rapid propagation technology of Chinese catalpa mostly adopts stem tips and stem segments as explants for propagation, but the induction rate is low and the differentiation efficiency is limited

Method used

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  • Method for regenerating plants by high-frequency induction of leaves of catalpa bungei
  • Method for regenerating plants by high-frequency induction of leaves of catalpa bungei
  • Method for regenerating plants by high-frequency induction of leaves of catalpa bungei

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Embodiment Construction

[0027] A kind of method for high-frequency induction regeneration plant of golden chrysalis leaf, its special feature is to comprise the following steps:

[0028] 1) Primary culture

[0029] Take the stem section of Catalpa catalpa with about 3 cm of axillary buds in the same year as an explant, first soak it with 0.05% by mass detergent for 10 minutes, and then rinse it repeatedly with tap water until there are no air bubbles. Transfer to the ultra-clean workbench, rinse with sterile water for 3 times, sterilize with 70% ethanol for 30 seconds, rinse with sterile water for 3 times, and then use 0.1% HgCl 2 +0.25% Tween-20 for 8 minutes, rinsed with sterile water for 5 times, and finally absorbed the surface moisture with sterile absorbent paper. Under the disinfection conditions, the contamination and browning and death of explants were relatively low. The stem segments were inserted into the primary medium, the culture temperature was 25±1°C, the light intensity was 1500-2...

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Abstract

The invention discloses a method for regenerating plants by high-frequency induction of leaves of catalpa bungei. The method comprises the steps of primary culture, subculture, adventitious bud induction, strong seedling rooting culture and the like. An adventitious bud induction culture medium comprises an improved N6 basic culture medium, 2.5 mg / L of 6-BA, 0.01 mg / L of IBA, 0.1 mg / L of TDZ, 30 g / L of sucrose and 7.0 g / L of agar. The method is simple and convenient to operate, rapid amplification of tissue culture seedlings of the catalpa bungei can be realized in a short time, technical support is provided for excellent planting resource protection and large-scale rapid production of the catalpa bungei, meanwhile, a foundation is laid for genetic engineering breeding of the catalpa bungei, and the method has very important significance for development of molecular mechanism research and germplasm resource improvement of the catalpa bungei.

Description

technical field [0001] The invention relates to a method for a high-frequency induced regeneration system of isolated leaves of Chinese catalpa, and belongs to the technical field of rapid propagation of catalpa tissue culture. Background technique [0002] Catalpa bungei C.A.Mey. is a deciduous tree of Bignoniaceae (Bignoniaceae) genus Catalpa. It is native to my country and has a cultivation history of more than 2,000 years. It is widely distributed in the Yangtze and Yellow River basins and has strong adaptability. , Wide range of uses, is an excellent landscaping tree species and precious furniture materials. Catalpa bungei var., also known as yellow catalpa and arrow catalpa, is an excellent geographical variety of catalpa. It is a native tree species in North China. It is naturally distributed in Shandong, Henan, Jiangsu, Anhui and other provinces. It has strong ability of diseases and insect pests and high survival rate of afforestation. It can be popularized in the N...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 宿红艳王磊张世忠孙亚东杨静静周文宇冯雪燕崔玉满
Owner LUDONG UNIVERSITY
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