3results about How to "Reduce ionic strength" patented technology

The invention relates to the technical field of environmental engineering, in particular to a fluorine ion crystallization recovery method based on a PCR (polymerase chain reaction) pellet reactor, which comprises the following steps: (1) measuring and recording the concentration of fluorine ions in wastewater to be treated, and adding a calcium salt agent and a special seed crystal according to the concentration of the fluorine ions in the wastewater to be treated; (2) adding a calcium salt agent and a special seed crystal, uniformly stirring and mixing, and adjusting the pH value of the wastewater to 6.0-7.5; (3) controlling the reaction time to be 10-30 minutes; (4) recycling fluorine-containing pellet crystals after the wastewater treatment is completed; the invention provides a fluorine ion crystallization recovery method based on a PCR (polymerase chain reaction) pellet reactor, which comprises the following steps: adding a calcium-containing composite chemical agent and a seed crystal into fluorine-containing wastewater to induce fluorine element crystallization to form microcrystals, and crystallizing layer by layer to form a fluorite-like layer as time goes on so as to realize recovery and reutilization of fluorine resources.

The invention provides a sphingosine supramolecular body and a preparation method and application thereof, and belongs to the technical field of cosmetics, and the sphingosine supramolecular body is prepared by dissolving hydroxy acid and sphingosine in water according to a molar ratio of 1: (0.5-5). According to the sphingosine supramolecular body provided by the invention, the supramolecular body is formed by self-assembly through the strong hydrogen-bond interaction between the hydroxy acid and sphingosine molecules, so that the ionic strength of the hydroxy acid can be effectively reduced, and the problem of poor water solubility of sphingosine can be solved.

The application discloses a method for semi-quantitative analysis of expression level of secreted protein of human joint cartilage cells by using immunoblotting technology. The method comprises the following steps: 1) preparing a secreted protein culture solution; 2) collecting a sample of the secreted protein culture solution; 3) performing dialysis desalting treatment on the sample; 4) concentrating the secreted protein culture solution to prepare a powder; 5) re-dissolving the secreted protein culture solution powder and quantifying the protein; 6) separating the protein components in the re-dissolved culture solution; and 7) detecting the expression level of the secreted protein in the re-dissolved culture solution sample by using immunoblotting method. The low-temperature dialysis and vacuum concentration technology can significantly improve the concentration of the secreted protein in the human joint cartilage tissue culture solution sample under the condition of maintaining the integrity of the protein structure, and is suitable for detecting the chemical fluorescence signal of the secreted protein in the sample by using the immunoblotting technology. The application provides an important cell reaction detection tool for studying the pathogenesis of bone and joint diseases caused by degenerative changes of cartilage tissue and researching and developing biological small-molecule drugs for treating the diseases.