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Purification method for sodium hyaluronate

A sodium hyaluronate and purification method technology, applied in the field of sodium hyaluronate purification, can solve the problems of high energy consumption, unfavorable large-scale production operations, and long time, and achieve the effects of increasing yield, reducing adverse reactions, and reducing absorption

Active Publication Date: 2012-06-27
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 3. After complexation and precipitation agglomeration, the dissociation time generally takes 8-10 hours. On the one hand, the sodium hyaluronate will degrade during the process and the energy consumption is high; on the other hand, it is not conducive to large-scale production operations.

Method used

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  • Purification method for sodium hyaluronate
  • Purification method for sodium hyaluronate

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Prepare sodium hyaluronate with the existing extraction method, take cockscomb enzymatic hydrolysis liquid and filter 400ml of medicinal liquid after activated carbon adsorption and filtration, take 1.6g of CPC and add a little water to dissolve, stir and mix with medicinal liquid, and complex and precipitate for 30 minutes. After the complex precipitation is complete, let the precipitate stand still, discard the supernatant, and prepare 65ml of 0.4mol / L dissociation solution to dissociate the complex precipitation for 5 hours. After the dissociation is completed, take an appropriate amount of purified water and dilute the dissociation solution To 260ml, stir while diluting, the complexed precipitate begins to precipitate, stop diluting when the dissociated solution is diluted to 390ml, continue to stir the complexed precipitate for 30min for secondary complexation, discard the supernatant after the complexed precipitation is completed, and wash the precipitate After one...

Embodiment 2

[0028] Sodium hyaluronate was prepared by the existing extraction method, and 1000ml of the medicinal solution was taken from the cockscomb enzymatic hydrolysis solution once activated carbon was adsorbed and filtered, 4.0g of CPC was dissolved in a little water, stirred and mixed with the medicinal solution, and complexed and precipitated for 30 minutes. After the complex precipitation is complete, let the precipitate stand still, discard the supernatant, and prepare 163ml of 0.4mol / L dissociation solution to dissociate the complex precipitation for 4 hours. After the dissociation is complete, take an appropriate amount of purified water and dilute the dissociation solution to 650ml, stirring while diluting, the complexed precipitate begins to precipitate, stop diluting when the dissociated solution is diluted to 1250ml, continue to stir the complexed precipitate for 30min for secondary complexation, discard the supernatant after the complexed precipitation is completed, and wa...

Embodiment 3

[0030] Sodium hyaluronate was prepared by the existing extraction method, and 1000ml of the medicinal solution was taken from the cockscomb enzymatic hydrolysis solution once activated carbon was adsorbed and filtered, 4.0g of CPC was dissolved in a little water, stirred and mixed with the medicinal solution, and complexed and precipitated for 30 minutes. After the complex precipitation is complete, let the precipitate stand still, discard the supernatant, and prepare 163ml of 0.4mol / L dissociation solution to dissociate the complex precipitation for 5 hours. After the dissociation is complete, take an appropriate amount of purified water and dilute the dissociation solution to 650ml, stirring while diluting, the complexed precipitate begins to precipitate, stop diluting when the dissociated solution is diluted to 1180ml, continue to stir the complexed precipitate for 30min for secondary complexation, discard the supernatant after the complexed precipitation is completed, and wa...

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Abstract

The invention relates to a purification method for sodium hyaluronate, comprising the following steps of: performing adsorption and filtration on comb enzymolysis solution once by active carbon, uniformly stirring and mixing the obtained filtrate with CPC (cetylpyridinium chloride) aqueous solution, performing complex-precipitation thoroughly, standing the precipitate, removing the supernatant, and stirring and dissociating the complex precipitate by dissociating solution; diluting the dissociated liquid to 2-8 folds of the volume thereof, performing complex-precipitation for the second time, removing the supernatant after the complex-precipitation is finished, washing the precipitate for 1-3 times, and stirring and dissociating the complex precipitate by the dissociating solution; and adsorbing the liquid dissociated for the second time by the active carbon, and then removing carbon and filtering, precipitating the filtrate by alcohol, dehydrating the obtained sodium hyaluronate, and vacuumizing. The sodium hyaluronate finished product prepared by the method is low in absorbance, few in impurities, high in yield, good in the physicochemical property of intermediate, and few in adverse reactions.

Description

Technical field: [0001] The invention relates to a purification method of sodium hyaluronate. Background technique: [0002] Hyaluronic acid (HA for short) is composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose-(1→4)-O-β-D-glucuronic acid bis A linear polysaccharide composed of sugar repeating units with the molecular formula (C 14 h 21 NO 11 )n, according to different tissue sources, the molecular weight range is 2×10 5 ~7×10 6 , the number of disaccharide units is 300 to 11,000 pairs. [0003] [0004] Commercial hyaluronic acid is generally in the form of sodium salt, white fibrous or powdery solid, with strong hygroscopicity, soluble in water, insoluble in organic solvents. The macromolecular network structure of sodium hyaluronate passes through the interaction with H 2 O forms hydrogen bonds to bind a large amount of water, and has the functions of forming various matrices, regulating osmotic pressure, regulating the transport of macromolecular substances, fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 张云陈亮马贤鹏
Owner SHANGHAI JINGFENG PHARMA
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