Ex-vitro rooting method for tissue culture seedlings of catalpa bungei

Abstract

The invention discloses an ex-vitro rooting method for tissue culture seedlings of catalpa bungei. The method comprises the following steps that 1) a rooting material is selected; 2) a wound of the rooting material is healed; 3) the tissue culture seedlings are subjected to cutting rooting; 4) the seedlings are tended in a nutrient block. According to the ex-vitro rooting method for the tissue culture seedlings of the catalpa bungei, firstly, an incision of the rooting material is subjected to wound healing treatment, then later rooting culture is conducted so that the tissue of the incision can be healed well, the risk that the incision is infected with bacteria is reduced, and in the later period, the seedlings can root better; the whole rooting culturing time is shortened, in addition,after rooting, the catalpa bungei seedlings can grow roots in a natural environment at the indoor temperature, the main root has a large number of fibrils, the root system activity is high, after 3-5days of new root growing, new roots can directly absorb nutrition in a medium to promote new leaf growing, and the survival rate of rooting is high. The method is suitable for the tissue culture seedlings of the catalpa bungei.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for rooting outside a bottle, in particular to a method for rooting outside a bottle for tissue culture seedlings of catalpa. Background technique [0002] Catalpa is a deciduous tree belonging to the Bignoniaceae catalpa. Under good site conditions, the height of the tree can reach 30 meters, and the diameter at breast height can reach more than 1 meter. Catalpa is native to my country, and it is a unique native tree species in my country. It is tall and straight, with a straight trunk, beautiful tree posture, and gorgeous flowers and colors. It is a tall flower-viewing tree. Because of its excellent material, it is known as the "King of Wood". Catalpa wood has a high value and a high yield rate. However, due to various reasons, it is now an endangered tree species. Catalpa is a deep-cultivation tree species, with few fibrous roots in the surface cultivation layer, and...

AI Technical Summary

Problems solved by technology

However, due to the influence of its genetic characteristics, the catalpa tree has the characteristics of self-fertilization in seed reproduction, so the germination rate is low, and the traits of the offspring are seriously separated.

Therefore, in order to keep the good traits of the female plant, only vegetative propagation can be carried out, and the vegetative propagation mode of cutting rooting has limited its application due to the low rooting rate; However, catalpa saplings grafted with catalpa are not resistant to repeated stubble, and the root system is easily infected with phylloxera nematode. Once the disease occurs, the losses will be heavy, and "big and small foot diseases" are prone to occur, and the trees are difficult to grow.

Catalpa seedlings propagated through tissue culture technology are self-rooted seedlings, and there is no problem of catalpa being infected with root-knot nematode disease. However, the rooting method of traditional catalpa seedlings needs to undergo rooting in the bottle (25-30 days) and rooting outside the bottle. There are three stages of seedling hardening (25-30 days) and nutrient pot seedling tending (20-30 days), and it takes 70-120 days to obtain finished seedlings, and the acquisition cycle of the entire finished seedlings is longer

In addition, the substrate used for rooting in traditional Chinese catalpa seedlings is agar medium with added sugar and minerals. The leaves of the plants do not have photosynthetic ability, and the roots are mostly fleshy roots. It takes a long time to harden the seedlings in the greenhouse. It adapts to the natural environment, and its survival rate is unstable and easily affected by the environment

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