Ex-vitro rooting method for tissue culture seedlings of catalpa bungei

A technology of tissue culture seedlings and external rooting, applied in the field of plant cultivation, can solve the problems of unstable survival rate, separation of offspring traits, self-fertilization, etc., achieve shortening rooting cultivation time, high rooting survival rate, and promote new leaf extraction raw effect

Inactive Publication Date: 2019-06-18
上海杉一植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the influence of its genetic characteristics, the catalpa tree has the characteristics of self-fertilization in seed reproduction, so the germination rate is low, and the traits of the offspring are seriously separated.
Therefore, in order to keep the good traits of the female plant, only vegetative propagation can be carried out, and the vegetative propagation mode of cutting rooting has limited its application due to the low rooting rate; However, catalpa saplings grafted with catalpa are not resistant to repeated stubble, and the root system is easily infected with phylloxera nematode. Once the disease occurs, the losses will be heavy, and "big and small foot diseases" are prone to occur, and the trees are difficult to grow.
Catalpa seedlings propagated through tissue culture technology are self-rooted seedlings, and there

Method used

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  • Ex-vitro rooting method for tissue culture seedlings of catalpa bungei
  • Ex-vitro rooting method for tissue culture seedlings of catalpa bungei
  • Ex-vitro rooting method for tissue culture seedlings of catalpa bungei

Examples

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Embodiment 1

[0026] A method for rooting out of a bottle of catalpa tissue culture seedlings, comprising the following steps:

[0027] 1) Selection of rooting material: select catalpa tree tissue culture seedlings with a plant height of 2-4cm, a subculture period of 30 days, and dark green leaves, remove the base leaves and axillary buds, and leave 3-5 fully expanded leaves on the top, Obtain rooting material A;

[0028] 2) Wound healing of rooting material: Coat the incision of rooting material A with plant wound healing agent, then insert it into medium M and cultivate it for 3 days. The culture conditions are: culture temperature 25°C, light intensity 3000Lux, light duration 12h / day , to obtain rooting material B; wherein, the medium M is: 60 mesh vermiculite and deionized water are sterilized at 121 ° C for 10 minutes, and the volume ratio of 60 mesh vermiculite: deionized water is = 1:1 . The plant wound healing agent is a mixture of a bactericide and an auxiliary agent, and the con...

Embodiment 2-6

[0033] Embodiment 2-6 is respectively a kind of flat external rooting method of catalpa tree tissue culture seedling, and its step is similar to embodiment 1, and difference only is that the technical parameter involved wherein is different, specifically see the following table for details:

[0034]

[0035]

[0036] The catalpa rooted seedling that makes by above-mentioned embodiment 2-6 method can grow root under room temperature natural environment, there is a large amount of fibrous roots on main root, and root system vigor is strong, and after 3-5 days new root sends out, directly absorbs the nutrition in the matrix and promotes new growth. The leaves are pumped, and the rooting survival rate is high, which can reach more than 98%. The leaf color is dark green, and the plant height is more than 6cm in 25-30 days.

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Abstract

The invention discloses an ex-vitro rooting method for tissue culture seedlings of catalpa bungei. The method comprises the following steps that 1) a rooting material is selected; 2) a wound of the rooting material is healed; 3) the tissue culture seedlings are subjected to cutting rooting; 4) the seedlings are tended in a nutrient block. According to the ex-vitro rooting method for the tissue culture seedlings of the catalpa bungei, firstly, an incision of the rooting material is subjected to wound healing treatment, then later rooting culture is conducted so that the tissue of the incision can be healed well, the risk that the incision is infected with bacteria is reduced, and in the later period, the seedlings can root better; the whole rooting culturing time is shortened, in addition,after rooting, the catalpa bungei seedlings can grow roots in a natural environment at the indoor temperature, the main root has a large number of fibrils, the root system activity is high, after 3-5days of new root growing, new roots can directly absorb nutrition in a medium to promote new leaf growing, and the survival rate of rooting is high. The method is suitable for the tissue culture seedlings of the catalpa bungei.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for rooting outside a bottle, in particular to a method for rooting outside a bottle for tissue culture seedlings of catalpa. Background technique [0002] Catalpa is a deciduous tree belonging to the Bignoniaceae catalpa. Under good site conditions, the height of the tree can reach 30 meters, and the diameter at breast height can reach more than 1 meter. Catalpa is native to my country, and it is a unique native tree species in my country. It is tall and straight, with a straight trunk, beautiful tree posture, and gorgeous flowers and colors. It is a tall flower-viewing tree. Because of its excellent material, it is known as the "King of Wood". Catalpa wood has a high value and a high yield rate. However, due to various reasons, it is now an endangered tree species. Catalpa is a deep-cultivation tree species, with few fibrous roots in the surface cultivation layer, and...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/15A01G2/10
Inventor 冒文娟
Owner 上海杉一植物科技有限公司
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