Cultivation method for micropropagation of catalpa bungei
A cultivation method and micro-propagation technology, applied in the field of asexual rapid propagation of forestry seedlings, can solve the problems of lack of catalpa tree seedlings, mixed varieties, long cultivation time, etc. Create simple and convenient effects
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Embodiment 1
[0037] Such as figure 1 As shown, the present embodiment provides a kind of cultivation method of Chinese catalpa micropropagation, carries out according to following step order:
[0038] 1) Acquisition of explants
[0039] Collect catalpa tree branches that are healthy and full of buds and cut them into 4cm branches, put them in a detergent solution prepared from 3g of washing powder and 1000g of water, and scrub with a brush. The parts with buds take a little longer to wash, and then Rinse the branches with running water for 1 hour; on the ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, rinse with sterile water for 4 times, then disinfect with 1‰ of mercury liter for 6 minutes, rinse with sterile water for 5 times, Drain the water, cut into 2 ~ 3cm long bud stems and inoculate them into medium A. Medium A includes: DKW medium is used as the basic medium, and the concentration of 1.2mg / L 6-BA (6- Benzylaminoadenine), concentration is the IBA (indole butyri...
Embodiment 7
[0060] The present embodiment has screened the culture medium used in the cultivation method of the catalpa micropropagation. The plant tissue culture is to use the basic medium as the basic carrier of the cultured tissue, and then add different hormone components on the basic medium to obtain The best state of cultivation, therefore very important for the screening of the basal medium of different cultivating tree species, the present embodiment uses DKW as the basal medium of the present invention, and compares with MS, 1 / 2MS as the basal medium, to catalpa The explant inoculation, transfer and subculture of the basic medium screening study were carried out, and the results are as follows:
[0061]
[0062] As can be seen from the above table, using DKW as the basic medium for catalpa tissue culture culture starts quickly, has high proliferation rate, good growth of bottle seedlings, and good rooting and survival rate. It is the best basic medium for catalpa tissue culture...
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