Cultivation method for micropropagation of catalpa bungei

A cultivation method and micro-propagation technology, applied in the field of asexual rapid propagation of forestry seedlings, can solve the problems of lack of catalpa tree seedlings, mixed varieties, long cultivation time, etc. Create simple and convenient effects

Inactive Publication Date: 2017-05-10
HUBEI FORESTRY SCI INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the invention is to overcome the lack of exi

Method used

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  • Cultivation method for micropropagation of catalpa bungei
  • Cultivation method for micropropagation of catalpa bungei
  • Cultivation method for micropropagation of catalpa bungei

Examples

Experimental program
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Embodiment 1

[0037] Such as figure 1 As shown, the present embodiment provides a kind of cultivation method of Chinese catalpa micropropagation, carries out according to following step order:

[0038] 1) Acquisition of explants

[0039] Collect catalpa tree branches that are healthy and full of buds and cut them into 4cm branches, put them in a detergent solution prepared from 3g of washing powder and 1000g of water, and scrub with a brush. The parts with buds take a little longer to wash, and then Rinse the branches with running water for 1 hour; on the ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, rinse with sterile water for 4 times, then disinfect with 1‰ of mercury liter for 6 minutes, rinse with sterile water for 5 times, Drain the water, cut into 2 ~ 3cm long bud stems and inoculate them into medium A. Medium A includes: DKW medium is used as the basic medium, and the concentration of 1.2mg / L 6-BA (6- Benzylaminoadenine), concentration is the IBA (indole butyri...

Embodiment 7

[0060] The present embodiment has screened the culture medium used in the cultivation method of the catalpa micropropagation. The plant tissue culture is to use the basic medium as the basic carrier of the cultured tissue, and then add different hormone components on the basic medium to obtain The best state of cultivation, therefore very important for the screening of the basal medium of different cultivating tree species, the present embodiment uses DKW as the basal medium of the present invention, and compares with MS, 1 / 2MS as the basal medium, to catalpa The explant inoculation, transfer and subculture of the basic medium screening study were carried out, and the results are as follows:

[0061]

[0062] As can be seen from the above table, using DKW as the basic medium for catalpa tissue culture culture starts quickly, has high proliferation rate, good growth of bottle seedlings, and good rooting and survival rate. It is the best basic medium for catalpa tissue culture...

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Abstract

The invention provides a cultivation method for micropropagation of catalpa bungei. The cultivation method comprises a step 1) of expalnt obtaining; a step 2) of change over culture; a step 3) of multiplication of subculture; a step 4) of cuttage culture; and a step 5) of seedling exercising and transplantation. A propagation material for the cultivation method for micropropagation of catalpa bungei comes from a matrix, namely a bred excellent seed tree, it is guaranteed that produced seedlings are all good stocks representing female parent clone, a phenomenon of variety complexity does not exist, a seedling culture propagation coefficient is remarkably improved, and the rooting rate, and the survival rate and the strong seedling rate of cutting seedlings are improved. Besides, the tissue culture rooting process of the catalpa bungei tissue culture seedlings is omitted, rootless seedlings with heights of 2-3 m are directly utilized to perform micro cuttage culture, and culture time and production cost are greatly saved while the seedling expanding propagation coefficient is ensured.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of forestry seedlings, and in particular relates to a method for cultivating catalpa micropropagation. Background technique [0002] The catalpa tree is majestic, tall and straight, with multi-colored flowers, excellent material, straight texture, not warped or cracked, corrosion-resistant and moisture-resistant. It is a precious and high-quality timber tree species unique to my country and a famous garden ornamental tree species. It has been known as the "king of wood" since ancient times. say. Catalpa has strong growth adaptability, salt and alkali resistance, acid resistance, sound attenuation, dust retention, adsorption of sulfur dioxide, chlorine and other toxic gases, and has no pollution to the environment. It has been recognized as a "human health tree species" by the United Nations World Health Association. With the increasing demand for wood in modern society and people...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G24/00A01G31/00A01H4/001A01H4/008
Inventor 张新叶李振芳陈慧玲杨杉张亚东彭婵黄国伟马林江周国清杜拾平
Owner HUBEI FORESTRY SCI INST
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