The invention discloses a culture method of cymbidium, aiming at solving the problems that when the cymbidium is bred by a tissue culture technique at present, mutation is inevitably produced, so that the seedling quality is influenced, and the breeding speed of protocorm-like bodies is limited. The culture method comprises the following steps: inducing the protocorm-like bodies by bud apical meristem, carrying out subculture multiplication of the protocorm-like bodies, carrying out differentiation on the protocorm-like bodies to produce adventitious buds, and carrying out strong seedling culture on the adventitious buds to obtain seedlings. After the culture method is used, the problem of mutation in the seedling production of the cymbidium can be effectively solved, the occurrence of mutation is reduced, the value-added ratio is increased, more than half of the subculture multiplication time is shortened, and the multiplication rate of the protocorm-like bodies is improved. The test proves that the value-added rate of the protocorm-like bodies is increased by 80-120%, the mutational rate is reduced by 5-9%, and the problem of contradiction between the value-added rate and the mutational rate of the protocorm-like bodies of the cymbidium can be effectively solved. The culture method has important significance for the rapid breeding and industrial large-scale production of the seedlings of the cymbidium, and has better market prospects and application values.