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Method for breeding mimosa

A technology of mimosa and formula, applied in the field of plant tissue culture, can solve the problems of low rooting rate, inconvenient operation, low differentiation rate and the like, and achieve the effects of increased rooting rate, easy operation and shortened occurrence time.

Inactive Publication Date: 2005-03-30
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after testing, this propagation method mainly has the disadvantages of using a wide variety of hormones, inconvenient operation, low differentiation rate, and low rooting rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Disinfection of explants: take mimosa seeds, soak them in 65% ethanol for 20 seconds, sterilize them in 0.1% mercuric chloride for 15 minutes, and rinse them with sterile water for 3 times;

[0020] (2) Germination of sterile seedlings: Inoculate the sterilized seeds on 1 / 2MS medium and cultivate them for 15 days to obtain sterile seedlings. The culture conditions are temperature 25±2°C, light intensity 1500Lux, light time 12h·d -1 (meaning "hour / day", the same below);

[0021] (3) Induction of callus: cut the hypocotyls of sterile seedlings, transfer them to MS medium supplemented with 2mg / L2, 4-D and 0.2mg / L 6-BA, and culture them for 30 days to induce callus. Wounded tissue, callus induction rate 100%;

[0022] (4) Differentiation of shoots: after one month, inoculate on the MS medium supplemented with 1mg / L 6-BA and 0.5mg / L TDZ, and when cultured for two months, the differentiation frequency is 40%. The number of seedlings on the top is 5-10;

[0023] (5) For...

Embodiment 2

[0026] Other operations and the control group are the same as in Example 1, except that in step (1), the mimosa seeds are taken and soaked in 75% ethanol for 60 seconds, sterilized in 0.1% mercuric chloride for 4 minutes, and rinsed with sterile water for 6 minutes. time; in the step (2), the culture time is 30 days; in the step (3), the culture time is 10 days; in the step (4), the medium formula is MS+2mg / L 6-BA, and the culture time is 30 days , the adventitious bud differentiation rate reached 60%; in step (5), the medium was changed to 1 / 2MS+1.0mg / L NAA, and in 20 days, the rooting rate was 64.2%. As the culture time prolongs, the rooting rate will be higher .

[0027] And the adventitious bud induction rate of the control group is only 8%, and the adventitious root occurrence rate is the highest 50%, which is obviously worse than the method of the present invention.

Embodiment 3

[0029] Other operations and matched groups are the same as in Example 1, except that in step (1), the Mimosa seeds are taken and soaked in 70% ethanol for 30 seconds, sterilized in 0.1% mercuric chloride for 8 minutes, and rinsed with sterile water for 5 minutes. time; in the step (4), the Mimosa callus is transferred to the B5 medium that has 2mg / L 6-BA added, and the adventitious bud differentiation rate reaches 70%; in the step (5), the medium is 1 / 2MS +1mg / L NAA, the culture time is 10 days, and the rooting rate is 64.2%.

[0030] And control group step (4) adopts additional hormone 2, the B5 medium of 4-D 0.5mg / L and 2mg / L 6-BA, adventitious bud induction rate is only 8%, adopts B5+2mg / L in step (5). In the culture medium of L IAA, the incidence of adventitious roots is only 50%, and the growth is slow, which is obviously worse than the method of the present invention.

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Abstract

A method for reproducing mimose includes disinfecting explant, geminating to obtain aseptic seedlings, inducing calli by use of the culture medium MS+2mg / L 2,4-D+0.2mg / L6-BA, inducing the generation of adventitious bud by use of the culture medium MS or B5+1.0-2.0 mg / L 6-BA+0-0.5 mg / L TD2, and rooting by use of the culture medium B5+2mg / L IAA or 1 / 2 MS+0.5-2 mg / L IBA or NAA.

Description

(1) Technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of mimosa in vitro culture by using tissue culture technology. (2) Background technology [0002] Plant tissue culture refers to the process of using any organ, tissue or cell of a plant to carry out sterile culture growth and development under artificial control conditions. The technology takes less materials, the cost of cultivating plant materials is low, the growth cycle is short, and the management is convenient. Utilizing the rapid propagation technology of plant tissue culture, a large number of plants that maintain the biological characteristics and genetic traits of the female parent can be reproduced in a short period of time. It is understood that there are nearly a thousand species of fast-growing plants in my country. There are several kinds of basic medium commonly used, such as MS, B5, White, N6, etc. In ti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈刚李玲
Owner SOUTH CHINA NORMAL UNIVERSITY
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