Styrax zhejiangensis in-vitro embryo induced cluster bud rapid propagation method

A benzoin, in vitro technology, which is applied in the field of tree seedling cultivation, can solve the problems of weak spreading ability, less natural regeneration of seedlings, serious flower drop and fruit drop, etc., and achieves the effect of shortening the production time and the breeding cycle.

Active Publication Date: 2020-02-07
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The seeds of Zhejiang benzoin have a certain natural reproductive ability, but under natural conditions, the flower and fruit drop is serious, there are obvious seed size years, the seed coat is thick, and it needs to be dormant in a thick humus layer to germinate, and the spreading ability is weak. In addition, the seeds contain oil, and birds and animals like to eat them, so the natural renewal of seedlings can germinate very little
Zhejiang benzoin grows slowly, and the annual growth of adult plants is only 10-20cm. In addition, the number of plants is very small, and cuttings are used for propagation. The number of spikes is limited, and the rooting rate is not ideal.
At present, there is no report about the rapid breeding method of Zhejiang benzoin both at home and abroad

Method used

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  • Styrax zhejiangensis in-vitro embryo induced cluster bud rapid propagation method
  • Styrax zhejiangensis in-vitro embryo induced cluster bud rapid propagation method
  • Styrax zhejiangensis in-vitro embryo induced cluster bud rapid propagation method

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Experimental program
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Embodiment 1

[0036] 1 Materials and methods

[0037] 1.1 Materials

[0038] 1.1.1 Plant material

[0039] The seeds of Zhejiang benzoin in the same year (harvested in Taohuawu, Longjiang, Jiande Forest Farm, Jiande City).

[0040] 1.1.2 Reagents

[0041] Basic medium includes WPM, MS, B5, N6 components, plant hormones include NAA, 6-BA, IBA, TDZ and GA, other reagents include sucrose, hydrolyzed casein and crystal agar.

[0042] 1.2 Method

[0043] 1.2.1 Seed detoxification

[0044] Shelled seeds ( figure 1 ) in a solution of detergent + Tween 80 (the amount of detergent added is about 0.5wt%, and the amount of Tween 80 added is about 0.2wt%) for 0.5-1h, then rinsed with running water for 0.5-1h, and turn Soak in 3% potassium permanganate for 30 minutes, then rinse with sterile water for 3 to 5 times, and then soak in sterile water for 48 to 72 hours; remove the hard seeds with a sheller in the ultra-clean bench The outer testa, and remove the inner testa attached to the endosperm w...

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Abstract

The invention relates to a tiny population plant-styrax zhejiangensis in-vitro embryo induced cluster bud rapid propagation method. According to the method, mature in-vitro embryos of styrax zhejiangensis are used as explants, and after pretreatment, the in-vitro embryos are directly induced to generate cluster buds; by bud multiplication culture and strong seedling culture, a great quantity of robust bud seedlings can be obtained. The method has advantages that seed dormancy of styrax zhejiangensis can be broken, cluster bud generating time is shortened, an average multiplication coefficientof the cluster buds reaches 6.5 times in two months, and the method is significant to expansion of styrax zhejiangensis which is a tiny population plant and establishment of a styrax plant tissue culture system.

Description

[0001] (1) Technical field [0002] The invention relates to a method for rapid propagation of clustered buds induced by isolated embryos of Zhejiang benzoin, a plant with a very small population, and belongs to the technical field of tree seedling cultivation. [0003] (2) Background technology [0004] Zhejiang Benzoin (Styrax zhejiangensis), a shrub or small tree, is a species of Benzoinaceae Benzoin. It is a very small population of wild plants unique to Zhejiang Province. It has a beautiful tree shape and fragrant flowers. It is a rare ornamental plant. Its flowers, fruits, and wood all have important economic value and can be used for landscaping, medicinal health care, and energy utilization. At present, it is only found in Taohuawu, Longjiang Forest District, Jiande Forest Farm, Zhejiang Province. Due to its extremely narrow distribution area, its number is rare, and it is endangered. The surveyed number in 2018 was only 106. It is extremely endangered and has been list...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李婷婷邱帅吴初平袁位高傅国林余裕龙盛卫星石从广
Owner ZHEJIANG FORESTRY ACAD
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