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Method for cultivating immature capsicum seeds and rapidly propagating seedlings

A technology of immature seeds, which is applied in the field of pepper immature seed cultivation and rapid seedling propagation, can solve the problems of low efficiency, long breeding cycle, and many pepper breeding processes, so as to reduce experimental steps, increase growth speed, and improve The effect of breeding efficiency

Active Publication Date: 2019-01-11
HUNAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for cultivating immature capsicum seeds and rapidly multiplying seedlings, so as to solve the technical problems of many capsicum breeding processes, long breeding cycles and low efficiency in the prior art

Method used

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  • Method for cultivating immature capsicum seeds and rapidly propagating seedlings
  • Method for cultivating immature capsicum seeds and rapidly propagating seedlings
  • Method for cultivating immature capsicum seeds and rapidly propagating seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The present invention provides a kind of capsicum immature seed culture and the method for rapidly multiplying seedling, comprise the steps:

[0031] (A) Acquisition of immature seeds: Take the green pepper 6421 fruits 25-30 days after field pollination, put them in a ziplock bag, seal the bag tightly, leave it at room temperature for 3 days, take it out, rinse it under running water, and use 75% alcohol Wipe the peel 2-3 times with a cotton ball. Cut the fruit in half lengthwise with a scalpel, and finally gently remove the immature seeds with tweezers for inoculation.

[0032] (B) Germination culture of immature seeds: Inoculate the immature seeds in the seed germination medium on the ultra-clean workbench and cultivate them until seedlings grow. The formula of the seed germination medium is 1 / 2MS medium (see Table 1)+0.5mg / L gibberellin+20g / L sucrose+6g / L agar powder, and the pH value is 5.8. The culture conditions are as follows: the culture temperature before the...

Embodiment 2

[0045] The immature seeds were inoculated in the seed germination medium on an ultra-clean bench and cultivated until seedlings grew. The medium formula is 0.7g / L ammonium nitrate+1.5g / L potassium nitrate+0.07g / L potassium dihydrogen phosphate+0.5mg / L gibberellin+20g / L sucrose+6g / L agar powder, other operations and conditions are the same as in Example 1. After 7 days of inoculation, it was found that the germination rate of the seeds in Example 2 and Example 1 all reached greater than 95%, indicating that it is feasible to replace the 1 / 2MS culture medium with this inorganic salt, and this method saves the experimental cost and reduces the experimental cost. step.

Embodiment 3

[0047] The capsicum immature seeds were inoculated in the medium supplemented with 0.5g / L streptomycin, and the experiment was not carried out on the ultra-clean bench, and other operations and conditions were the same as in Example 2. After 3 days of inoculation, it was observed that adding low-concentration streptomycin to the culture medium was equivalent to the inoculation effect in the ultra-clean workbench, and the seed contamination rate in Example 3 and Example 2 was less than 1%; after 7 days of inoculation, the seed germination rate was observed It was found that the addition of streptomycin did not affect the germination of the seeds, and the germination rates of the seeds in Example 3 and Example 2 were both greater than 95%, indicating that the method of adding streptomycin can simplify the inoculation process.

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Abstract

The invention provides a method for cultivating immature capsicum seeds and rapidly propagating seedlings. The method comprises the following steps: A) obtaining immature seeds: cutting every washed green capsicum into two halves, taking out the immature seeds, and inoculating with the immature seeds; B) performing germination culturing on the immature seeds: inoculating a seed germination mediumwith the immature seeds, and culturing the immature seeds; C) carrying out primary culturing: inoculating a primary medium with hypocotyls containing growth points, and carrying out culturing until adventitious buds appear; D) subculturing: transferring the adventitious buds to a subculture medium in order to elongate axillary buds; E) carrying out rooting culturing: inoculating a routing medium with explants with the elongated axillary buds, and performing culturing; and F) hardening and transplanting obtained tissue culture seedlings. The culture of the immature seeds adopts an open culturing technology, and inoculation does not need to be carried out on a super clean workbench, so the method is simple and convenient to operate, and is not limited by the site. The method can be combinedwith the propagation of capsicum seedlings to obtain a large number of plants in a short time, so the capsicum germplasm resource creation and new variety breeding cycle is significantly shortened.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating immature pepper seeds and rapidly multiplying seedlings. Background technique [0002] Pepper (Capsicum annuum L.) is the third largest vegetable crop after beans and tomatoes in the world. It has strong adaptability, wide cultivation area and rich nutrition, and is deeply loved by people. According to the statistics of the bulk vegetable industry technology system of the Ministry of Agriculture, my country's existing chili planting area is 1.5-2 million hectares, ranking first in vegetables. Peppers are native to Central and South America. They like temperature and light but are not cold-resistant. They also have a long growth cycle. Generally, only two crops can be planted a year in my country, and the breeding cycle for innovative varieties is long. Therefore, shortening the breeding time and improving the breeding efficiency are more and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 周书栋杨博智马艳青欧立军刘峰郑井元陈文超张竹青李雪峰杨莎梁成亮李鑫戴雄泽邹学校
Owner HUNAN VEGETABLE RES INST
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