Method for utilizing formamide deionized for promoting somatic embryogenesis of cunninghina lanceolata

Abstract

The invention discloses a method for utilizing formamide deionized for promoting somatic embryogenesis of cunninghina lanceolata. Embryonic calluses with a good subculture state are inoculated to a somatic embryo inducing culture medium, dark culture is conducted in an incubator, and the somatic embryogenesis of the cunninghina lanceolata is induced and promoted, wherein a 1/2DCR serves as a basicculture medium of the somatic embryo inducing culture medium, and FD with the concentration not exceeding 0.1134 is added. According to the method, as is proved by experiments, the low-concentrationFD can accelerate the somatic embryogenesis of the cunninghina lanceolata, and the high-concentration FD can cause browning death of the embryonic calluses of the cunninghina lanceolata. When the FD concentration is 0.1134, the somatic embryogenesis time can be shortened to 35 d. The somatic embryogenesis of the cunninghina lanceolata can be efficiently and quickly promoted, and the practicabilityis very high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for promoting somatic embryogenesis of Chinese fir by utilizing deionized formamide (Formamide Deionized, FD). Background technique [0002] Chinese fir (Cunninghina lanceolata) belongs to the fir genus of the family Fir, and is one of the important timber tree species in my country. It grows fast, has a straight and round stem shape, is light, soft and delicate in texture, easy to process, beautiful in texture, has a special aroma, and is neither warped nor cracked. Anti-moth and rot-resistant, it is an excellent raw material for construction, furniture, bridges, shipbuilding, papermaking and wood fiber industries [Ren Haiqing, Huang Anmin, Liu Junliang, Fei Benhua. Research progress and suggestions on the processing and utilization of Chinese fir [J]. Timber Industry, 2006 (01): 25-27.]. At present, there are mainly three ways to propagate Chine...

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Problems solved by technology

At present, there are mainly three ways to propagate Chinese fir seedlings: seeds, cuttings and traditional tissue culture [Wen Yafeng. Research on induction and subculture of Chinese fir embryonic tissue based on immature zygotic embryos [D]. Fujian Agriculture and Forestry University, 2011.], But the seed yield and seed sowing quality of improved seeds are unstable, the supply of high-grade improved varieties above the second generation is insufficient, and the seed sowing quality is poor, especially the seeds have many astringent seeds, which usually account for 40% to 60% of the weight [Zheng Renhua. Advances in research on genetics and breeding of Chinese fir. and countermeasures [J]. World Forestry Research, 2005 (03): 63-65.]; and cutting seedlings are limited by the number of mother tree spikes, site space and rooting rate, etc., and its scale is difficult to meet modern forestry production. There is a large demand for Chinese fir seedlings; in addition, traditional tissue culture root induction is more difficult, and the number of induced roots is small and weak, which affects the quality of seedlings and the success rate of afforestation to a certain extent

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