Plant extract with herbicidal activity and application of plant extract
A technology of herbicidal activity and extracts, applied in the fields of application, plant growth regulators, herbicides and algicides, etc., to achieve significant social and economic benefits
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Embodiment 1
[0035] Example 1 Preparation of bald fir organic solvent extract——methanol extract
[0036] The stem bark of Pine fir is dried at 50°C, crushed and passed through a 60-mesh sieve, and then the dry powder is placed in a container, and 3 times the volume of methanol (m / v) is added for extraction for 48-72 hours. The extraction was repeated 3 times, and the filtrates were combined and concentrated to obtain the methanol extract of bald fir.
Embodiment 2
[0037] Example 2 Preparation of bald fir steam distillate - essential oil
[0038] Sieve the bark of the stem bark of Chinese fir balata after crushing, put 500g of dry powder into a 1000mL round bottom flask, add 800mL of double-distilled water, and heat it in an electric furnace for steam distillation to obtain the essential oil of Chinese fir baldness.
Embodiment 3
[0039] Example 3 The weeding experiment of bald fir on red thistle, ghost needle grass and crabgrass
[0040] 1. Experimental method
[0041] (1) Experimental plants: take red thistle (dicot weed), ghost needle grass (dicot weed), crabgrass (monocot weed) as examples for research.
[0042](2) In order to clarify the herbicidal activity of bald fir, the "small cup method" was used to measure its inhibitory effect on different weeds. Put 2-3 layers of glass beads at the bottom of a disposable small plastic cup, add 5mL of a certain concentration of bald fir methanol extract, put 10 germinated seeds in each small cup, seal it with plastic wrap, and leave some small holes on the film for ventilation . Set the concentration gradient as: 200, 100, 50, 25, 12.5 and 0 μg / mL, and repeat each concentration three times. After the treatment, put the cuvette into a light incubator at a temperature of 26° C., a light of 4000 lux, and a photoperiod of 16:8.
[0043] (3) After 7 days, roo...
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