Idiopathic male infertility-related medium-chain fatty acid marker in serum and detection method and application thereof

A medium-chain fatty acid and male infertility technology, applied in the fields of analytical chemistry and clinical medicine, can solve problems such as low sensitivity and complicated experimental steps

Inactive Publication Date: 2017-04-05
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

NMR technology is characterized by no damage to the components to be measured, simple sample pretreatment, but low sensitivity; gas chromatography-mass spectrometry has good sensitivity and reproducibility, but derivatization methods are generally used for sample pretreatment , making the experimental steps more complicated

Method used

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  • Idiopathic male infertility-related medium-chain fatty acid marker in serum and detection method and application thereof
  • Idiopathic male infertility-related medium-chain fatty acid marker in serum and detection method and application thereof
  • Idiopathic male infertility-related medium-chain fatty acid marker in serum and detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Embodiment 1: Research object selection and group basis

[0106] The subjects of this part of the study are from the first-diagnosed idiopathic male infertility cases and healthy childbearing controls in the Affiliated Hospital of Nanjing Medical University. The research content and the informed consent form were approved by the Ethics Committee of Nanjing Medical University and complied with the requirements of relevant laws and regulations. Cases and controls signed the informed consent after understanding the content. All subjects underwent a complete physical examination and completed a questionnaire including basic personal information, living habits, occupational and environmental exposures, genetic risk factors, sexual and reproductive function, disease history, and physical activity. In the first stage, 113 idiopathic male infertility cases and 113 healthy controls were included; in the second stage, 15 idiopathic male infertility cases and 15 healthy controls ...

Embodiment 2

[0137] Example 2: UPLC-MS metabolomics biomarker screening for idiopathic male infertility

[0138] 1. Sample pretreatment

[0139] 1.1. Take 10 μL of serum, add 10 μL of internal standard A, add 10 μL of internal standard B, add 10 μL of internal standard C, add 40 μL of methanol (reagent A), and vortex for 30 seconds.

[0140] 1.2. Centrifuge at 16000g at 4°C for 15min in a centrifuge, transfer the supernatant to a 1.5mL imported EP tube, and concentrate the supernatant to dryness in a centrifugal concentrator at room temperature.

[0141] 1.3. Reconstitute with 5 μL ultrapure water (reagent D) and wait for analysis.

[0142] 2. Instrument testing

[0143] 2.1. Analytical instruments: UPLC Ultimate 3000system (Dionex) high-performance liquid chromatography; Q-Exactive high-resolution mass spectrometer.

[0144] 2.2. Liquid phase conditions:

[0145] 2.2.1 The liquid chromatographic column is a Hypersil GOLD C18 chromatographic column (100mm×2.1mm, particle size 1.9μm, Th...

Embodiment 3

[0158] Example 3: Stability analysis of capric acid and dodecanoic acid in serum

[0159] The method of Example 2 was used to evaluate the stability of capric acid and dodecanoic acid levels in serum (the interval was 2 weeks). The results showed that the measured levels of capric acid and dodecanoic acid in serum were stable ( figure 2 ), with properties as diagnostic / monitoring markers.

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Abstract

The invention belongs to the field of analytical chemistry and clinical medicine and discloses an idiopathic male infertility-related medium-chain fatty acid marker in serum and a detection method and application thereof. The marker is capric acid and/or lauric acid, and an UPLC-Q exactive MS method is adopted for detection. The marker can be used for auxiliary diagnosis and monitoring of idiopathic male infertility, has high sensitivity and specificity, and has clinic promotion value.

Description

[0001] field of invention [0002] The invention belongs to the fields of analytical chemistry and clinical medicine, and relates to medium-chain fatty acid markers in serum related to idiopathic male infertility and a detection method and application thereof based on UPLC-Q exactive MS. Background technique [0003] About 15% of couples in the world are plagued by infertility, and about 50% of them are male infertility. There are many causes of male infertility, and the same patient may have a single cause, or two different or related causes successively. However, there is still a considerable proportion of male infertility that cannot explain the cause, which is called idiopathic male infertility, which brings great difficulties to the diagnosis and treatment of the disease. The WHO standard for male infertility is that the husband and wife have lived together for more than 1 year after marriage without taking contraceptive measures, and the woman is infertile due to the ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2800/34
Inventor 陈敏健夏彦恺陆春城肖甜胡艳辉周作民陈道桢吴笛王心如
Owner NANJING MEDICAL UNIV
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