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Gene detection method of sperm cell apoptosis related gene

A gene detection and sperm cell technology, applied in the field of molecular biology, can solve problems affecting FASLG apoptosis signal transduction and other issues

Inactive Publication Date: 2014-03-19
上海中优医药高科技股份有限公司
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Problems solved by technology

FASLG C844T polymorphism may affect the expression of FASLG, and then affect the FASLG-mediated apoptosis signal transduction

Method used

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  • Gene detection method of sperm cell apoptosis related gene
  • Gene detection method of sperm cell apoptosis related gene
  • Gene detection method of sperm cell apoptosis related gene

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Embodiment Construction

[0083] The purpose of the present invention is to provide a method for detecting genes related to sperm cell apoptosis, and combine the specific environmental exposure factors and lifestyle factors related to azoospermia and oligospermia in the Chinese population to evaluate the risk of male infertility. predictive evaluation.

[0084] To achieve the above objectives, the present invention provides a method for detecting sperm cell apoptosis-related genes. The specific steps are:

[0085] Step 1: Types of samples tested and sampling methods

[0086] Use a sampling swab to swab the left and right cheeks in the mouth at least 40 times up and down to ensure that sufficient cell samples are collected;

[0087] Step 2: Extraction method of genomic DNA

[0088]The genomic DNA of each oral epithelial cell sample is extracted by silica gel adsorption method, and the time is 2 hours to 2.5 hours. After electrophoresis detection, clear white bands can be seen with the naked eye to ju...

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Abstract

The invention discloses a gene detection method of a sperm cell apoptosis related gene. The gene detection method is characterized in that detection of the sperm cell apoptosis related gene is provided for all subjects by simultaneously detecting and analyzing SNPs site gene portability of individual NOS3 gene and FASLG gene, and forecast evaluation on a male infertility risk is carried out by combining with unique environmental exposure factors and lifestyle factors related to azoospermia and oligospermia in Chinese population.

Description

technical field [0001] The invention relates to a method for detecting sperm cell apoptosis-related genes, which can be used to evaluate the detection of sperm cell apoptosis-related genes, and belongs to the technical field of molecular biology. Background technique [0002] Azoospermia and oligospermia are one of the most common causes of male infertility. According to the statistics of the World Health Organization, about 10% to 15% of couples of childbearing age in the world cannot have normal fertility due to various factors, of which male factors account for half, and 15% to 20% of male causes are caused by azoospermia. At present, the deletion, mutation and genetic polymorphism of spermatogenesis-related genes are considered to be important genetic causes of male spermatogenesis disorders. [0003] The process of spermatogenesis is precisely regulated by many genes, and the deletion or mutation of genes can lead to the interruption of spermatogenesis. Sperm maturati...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 毛丹丹王校
Owner 上海中优医药高科技股份有限公司
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