Rhabdomyosarcoma Fusion Gene Related Circular RNA Molecular Marker and Its Application
A technology of rhabdomyosarcoma and molecular markers, applied in the field of biomedical testing, can solve problems such as the specific mechanism to be elucidated, and achieve high detection rate and sensitivity
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[0034] This embodiment provides a molecular marker of rhabdomyosarcoma fusion gene-related circular RNA, said marker is F-circP3F, and the nucleotide sequence of the marker is shown in SEQ ID No.1. The molecular markers can be used in the diagnosis of rhabdomyosarcoma products.
[0035]The design idea of this application is: when the applicant uses RT-PCR technology to amplify the fusion gene PAX3-FOXO1 and its related circ RNA in the acinar RMS, it is speculated that PAX3-FOXO1 may be composed of intron 4 and intron 4 of PAX3 during the transcription process. Intron 2 of FOXO1 is complementary paired to form a circular lariat, and the intron is back-spliced to form a circular RNA associated with the fusion gene. Then, by optimizing the primer design and linear RNA digestion, a positive target fragment was amplified in the acinar RMS cell line using divergent primers, and it was confirmed as a fusion by Sanger sequencing the PCR product and comparing it with the NCBI datab...
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