28 results about "Proliferation differentiation" patented technology

The application discloses a culture medium for tissue culture of Limosella australis, which comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium. The primary culture medium is MS+6BA 0.4-0.6 mg / L+NAA 0.04-0.06 mg / L+AC 0.8-1.2 mg / L+2,4-D 0.04-0.06 mg / L. The proliferation and differentiation culture medium is modified MS+6BA 0.09-0.11 mg / L+NAA 0.04-0.06 mg / L+AC 1.5-2.5 mg / L. The rooting culture medium is 1 / 2MS+IAA 0.09-0.11 mg / L+AC 2.5-3.5 mg / L. The technical scheme has the highest callus induction rate of 86.7%, the highest differentiation rate of 86.7% and the highest rooting rate of 93.3%.

The invention discloses a skeletal muscle repair method and system based on muscle satellite cell regulation and control. The method comprises the following steps: performing proliferation and differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation and control map; determining a collaborative repair time window according to the atlas, extracting difference response deviation correction parameters to form an activity correction factor, and performing repair intensity correction on the collaborative repair time window to generate a self-adaptive repair window; carrying out exercise prescription adaptation on the self-adaptive repair window, identifying a satellite cell activation regulation and control channel, obtaining activation delay time through activation efficiency mapping, and delimiting a response level according to the activation delay time to generate layered repair configuration; activation delay evaluation is conducted on layered repair configuration to determine a preferred activation mode, dynamic activation characteristics are extracted based on optimal activation time window data to form an intervention execution sequence, a skeletal muscle repair execution instruction is output in combination with an exercise prescription parameter table, and dynamic collaborative adaptation of satellite cell repair state monitoring and training scheme parameters is achieved.

The present application relates to a method for inducing pluripotent cells to proliferate and / or differentiate into megakaryocyte progenitor cells (MKPs), comprising adding a human platelet lysate (hPL) and an HDAC inhibitor to an MKP differentiation medium. The present application also provides a culture medium used in the method, and a composition comprising the culture medium.

The invention discloses a cell culture medium supplement as well as a preparation method and application thereof, and relates to the technical field of cell biology. The supplement comprises DL-alpha-tocopheryl acetate, linolenic acid, ethanolamine, L-carnitine hydrochloride, 3, 3 ', 5-triiodoL-sodium thyrosinate, and serum albumin. As a cell culture additive with definite chemical components and simple composition, the cell culture additive can replace a traditional culture system with unknown chemical components or too complex composition, and is used for supporting proliferation, differentiation and characterization analysis of cells. The culture medium additive is clear in chemical component, simple to prepare, stable in effect and relatively low in cost, can promote in-vitro proliferation and oxidation resistance of cells including human embryonic stem cells and mesenchymal stem cells, and has important application value in actual production.

The present application relates to a method for inducing proliferation and / or differentiation of pluripotent cells into megakaryocyte progenitor cells (MKP), comprising adding human platelet lysate (hPL) and an HDAC inhibitor to an MKP differentiation medium. The invention also provides a culture medium used by the method and a composition containing the culture medium.

The application discloses application of a polysaccharide wrapping film in preparation of a cell culture product and belongs to the technical field of cell culture food. The application specifically forms the polysaccharide wrapping film on the periphery of a cell inoculation support after the cell inoculation support, can relieve the phenomenon that cells fall off from the support during culture, and further guarantees effective proliferation and differentiation of muscle stem cells and / or fat stem cells on the support, thereby providing a new solution approach for efficient and standardized production of the cell culture product.

The utility model relates to the technical field of medical instruments, and discloses a full-porous cervical vertebra fusion cage which comprises a machining area and a porous area. The machining area is provided with a threaded hole for leading in a surgical instrument; the porous area comprises an upper surface, a lower surface, a front surface, a rear surface and two side surfaces; and the upper surface and the lower surface are provided with anti-skid surfaces. The threaded hole in the machining area is used for being matched with a surgical instrument matched with the threaded hole, and surgical implantation is facilitated. The porous area provides more growth space for cells, adhesion, migration, proliferation and differentiation of the cells are facilitated, and finally the bone ingrowth effect is achieved. The full-porous cervical vertebra fusion cage has the mechanical property matched with the human body structure and good biocompatibility, can form a tight bonding interface with the human body tissue, improves the combination stability of the fusion cage and the human body tissue, has the mechanical property matched with the human body tissue, and can be applied to clinical treatment of cervical degenerative diseases.

The present invention relates to 1,2-dicarboxamide compounds of Formula (I); pharmaceutically acceptable salts, pharmaceutically acceptable stereoisomers, N-oxides or combination thereof, wherein 'X', 'Y', 'Z', ring A, ring B, R1, R2, R3, R4, R5, R6, 'm' and 'n' are as defined herein. The present invention also relates to 1,2-dicarboxamide compounds that modulate cellular activities like proliferation, differentiation, adhesion, migration and apoptosis by modulating protein kinase enzymatic activity. In particular the invention relates to compounds which inhibit, regulate, and / or modulate tyrosine kinases such as FLT1 (VEGFR1), FLT4 (VEGFR3), KDR (VEGFR2), MET (C-Met), MERTK (c-Mer), RET, AXL, TEK (TIE 2) and EGFR. The present disclosure relates to 1,2-dicarboxamide compounds for use in modulating kinase enzymatic activity and accordingly modulating kinase-dependent associated diseases and conditions such as cancers like Thyroid carcinoma, Ovarian carcinoma, Pancreatic carcinoma, Prostatic carcinoma, Renal cell carcinoma, Hepatocellular carcinoma, Breast carcinoma, Colorectal carcinoma, Oral squamous cell carcinoma, Colorectal, Lung adenocarcinoma or Endometrial cancer.

The application belongs to the field of biomedical science and neurology, and particularly relates to application of CHCHD2 gene and a protein coded by the CHCHD2 gene in preparation of a product for promoting proliferation and differentiation of neural stem cells. The application proves that the CHCHD2 gene and the protein coded by the CHCHD2 gene are highly expressed in in-vivo neural development and in-vitro neural differentiation process; and that knocking down or over-expressing the CHCHD2 gene or the protein coded by the CHCHD2 gene has a certain regulating effect on proliferation and differentiation of neural stem cells. The application provides a new scheme for preparation of a product for promoting proliferation and differentiation of neural stem cells, and provides a new target for drug research and development of neural regeneration and repair treatment of central nervous system development deficiency diseases, neurodegenerative diseases, cerebrovascular diseases, craniocerebral and spinal cord injuries.

This application proposes a method for three-dimensional cell culture using an injectable thermosensitive hydrogel. Cells are mixed with a thermosensitive hydrogel precursor solution and incubated at 34-37°C for 1-10 minutes, followed by the addition of cell culture medium. After culture, a vitamin B6 derivative is added for degradation, yielding a cell suspension. The cultured cells are then separated by centrifugation. Using a hydrogel with a rapid sol-gel phase transition provides cells with a three-dimensional support environment close to the in vivo environment, enabling cells to grow, proliferate, differentiate, and interact with other cells and the matrix under similar physiological conditions, achieving high-quality in vitro cell culture. Specifically, the three-dimensional structure is less affected by temperature during culture, which is beneficial for stable cell culture. After culture, the gel structure degrades rapidly and completely, allowing for rapid cell recovery; this is a high-quality cell culture method.

The application belongs to the field of tissue repair porous material research, and discloses a 3D printing cell culture chip for high-throughput structure screening and a preparation method and application thereof. The chip is formed by 3D printing and comprises a cell culture block, an anti-adhesion belt, a cell isolation groove and a substrate. The cell culture block is composed of a structural matrix of unit structures and / or parallel groups thereof, at least one of which has a gradient change in curvature, porosity, pore size and connected pore size. The isolation groove contains the anti-adhesion belt. The 3D printing cell culture chip for high-throughput structure screening can realize the detection and analysis of the adhesion, proliferation and differentiation of various cells on the high-throughput structural matrix under the same time and the same culture environment, and realizes rapid structure screening. The chip is integrated with a variety of non-cytotoxic materials combined with 3D printing technology, and is applied to the structure optimization research work of various tissue repair materials, thereby improving the work efficiency of structure screening.

The invention discloses a culture method of cornu cervi pantotrichum stem cells, and belongs to the technical field of tissue engineering. The culture method comprises the following steps: taking antler stem periosteum tissue, refrigerating and transporting the antler stem periosteum tissue through a special transportation preserving fluid, cutting, cleaning and centrifuging to obtain tissue precipitate; resuspending the precipitate by using an optimized serum-free culture medium, inoculating to a culture dish pre-coated with gelatin according to a specific density, standing and culturing, and removing non-adherent cells; after trypsinization and centrifugation, cells are resuspended by using a sorting buffer solution, and a cell population of a specific surface marker is screened by using a flow cytometry sorter, so that the antler stem cells are obtained; and finally, carrying out passage amplification under a low-oxygen condition to obtain the pilose antler stem cells after passage amplification. By optimizing a transportation and preservation system, a culture environment and a sorting process, the purity, the activity and the proliferation and differentiation capacity of the pilose antler stem cells are effectively improved, the process standardization degree is high, and reliable technical support is provided for application of the pilose antler stem cells in the fields of tissue engineering, regenerative medicine and the like.

The invention discloses application of combination of CD40L and cytokines in promotion of in-vivo or in-vitro proliferation and / or differentiation of HIS mouse B cells. The invention also discloses a construction method of the fully human monoclonal antibody development platform. The construction method comprises the following steps: firstly, taking an HIS mouse immune antigen as a first signal; and giving CD40L to the HIS mouse, taking a cell factor as a second signal, taking the spleen, and sorting B cells which are specifically combined with the antigen, so that the antigen-specific antibody can be screened out. According to the invention, an effective second signal combination is clearly screened out in vitro, and B cells are promoted to differentiate towards plasma mother cells. In vivo, a scheme of'antigen + CpG sensitization-> 24 hours-> second signal intravenous injection 'is adopted, so that the proportion and total number of antigen-specific B cells are remarkably improved, the antigen-specific B cells are promoted to be converted from TR / Naive to Bmem (CD38-), and meanwhile, the antigen-specific antibody titer is improved.

The invention provides a method for drawing a sepsis immune cell dynamic change map and application, and belongs to the technical field of sepsis treatment. On the basis of the scRNA-seq technology, the immune library is constructed, the immune microenvironment is more comprehensively analyzed by adopting the scVDJ-seq sequencing technology, the cloning source of a single cell can be accurately monitored, the proliferation, differentiation and mutation paths of the cloned cell can be reconstructed, and the microenvironment with the immune function change can be more accurately obtained. The map drawn by the method can allow clinical workers to more accurately evaluate the immune state of the patient through related marker genes, so that dynamic change capture can guide the treatment opportunity of immune regulation of the sepsis patient in clinic, and accurate treatment of the sepsis immune function can be realized.

The invention relates to a particle acellular matrix and a preparation method and application thereof.The particle acellular matrix comprises acellular matrix particles with gradient particle sizes and can be used for promoting skin wound healing, and the particle acellular matrix makes full contact with a complex wound surface and is degraded in a staged mode; a favorable microenvironment can be continuously provided for migration, growth, proliferation and differentiation of cells, so that wound healing is promoted.

The invention provides application of LncRNA as an ovarian cancer biomarker and a therapeutic target, and belongs to the field of biotechnology and medicine. The LncRNASLC8A1-AS1 is used as an ovarian cancer marker to positively regulate the occurrence and development of ovarian cancer, proliferation and differentiation of ovarian cancer cells can be inhibited by inhibiting the expression of the LncRNASLC8A1-AS1, an effective molecular marker is provided for diagnosis, disease assessment and prognosis of ovarian cancer, and a new target is also provided for treatment of ovarian cancer.

The invention belongs to the field of biological materials and nervous tissue engineering, and provides a mimic peptide CIDPC modified magnetic directional freeze-drying scaffold as well as a preparation method and application thereof, and the mimic peptide CIDPC modified magnetic directional freeze-drying scaffold has a good directional topological structure and biocompatibility. Through the synergistic effect of an external magnetic field and the mimic peptide, proliferation, differentiation and directional migration of nerve cells can be effectively promoted, angiogenesis is promoted, in-vivo inflammation is regulated and controlled, axon regeneration and angiogenesis are stimulated, and sensory and motor neuron regeneration is stimulated, so that a remarkable effect is shown in spinal cord injury repair; and a new thought and a new method are provided for nervous tissue engineering injury repair.

The invention provides a casein source 3D printing bio-ink and a preparation method of a biological scaffold and cell culture meat. The bio-ink is prepared from the following raw materials in parts by weight: 15.6 parts of gelatin; 2.6 parts of sodium alginate; 4.2 parts of casein; 52.5 parts of a sodium hydroxide deionized water solution with a pH value of 8; and 87.5 parts of a sterile 1 * PBS buffer solution. The ink is subjected to extrusion type 3D printing and calcium chloride crosslinking to obtain a biological scaffold; c2C12 cells are inoculated on the support, and cell culture meat is obtained through proliferation-differentiation culture. The problems that existing 3D printing bio-ink is single in performance and poor in component adaptability, a biological scaffold material is insufficient in mechanical stability, and nutritional ingredients are deficient are solved. The prepared bio-ink has excellent mechanical properties and printing adaptability, and provides key technical support for industrial preparation of cell culture meat.

The application belongs to the technical field of biomedical materials, and particularly relates to a triple helix self-assembled recombinant humanized type I collagen fiber implant, a preparation method and application thereof. The application firstly provides a recombinant humanized type I collagen, which has a unique triple helix structure and excellent self-assembly properties. Secondly, the application optimizes the self-assembly process of the recombinant humanized type I collagen to prepare a recombinant humanized type I collagen implant with ordered arrangement of collagen fibers. The implant has excellent injectability, durability, high biological safety, no pyrogen and hemolysis risk, low immunogenicity, can significantly promote the adhesion, migration, proliferation and differentiation of human skin fibroblasts, can significantly promote the adhesion, migration and proliferation of chondrocytes, and can promote the chondrogenic differentiation of bone marrow mesenchymal stem cells, and can be applied to the fields of skin, cartilage, bone and the like.

The application provides application of PDGF-D in regulation of stem cell proliferation and / or differentiation. PDGF-D is combined with a PDGFRbeta receptor in stem cells to activate a downstream Erk channel to regulate cell stemness, and the application regulates expression of the PDGF-D, thereby effectively regulating proliferation and differentiation activities of embryonic stem cells.

This invention discloses the application of the PINK1 gene in porcine trophoblast cells and a method for constructing cell lines. This invention regulates the proliferation, differentiation, and autophagy of porcine trophoblast cells by controlling the expression level of the PINK1 gene, thereby enhancing placental function and efficiency. Induced expression of the PINK1 gene activates the Parkin-P62-LC3B pathway, promotes mitophagy, increases the expression of Mgst1, CTBP2, and IGFBP3 genes, promotes cell proliferation, inhibits apoptosis, and enhances cellular antioxidant capacity. Knockout of the PINK1 gene produces the opposite effect. This invention provides methods for constructing PINK1-induced expression and knockout cell lines. Stable porcine trophoblast cell lines offer new technical means for studying porcine placental development mechanisms, improving placental efficiency, and enhancing sow reproductive performance, which is of great significance to the development of the pig industry.

The application discloses an sgRNA molecule for knocking down or knocking out an IGFBP3 gene and application thereof in increasing deer antler weight, and relates to the technical field of deer antler regeneration. Knocking out the IGFBP3 gene in the caprine horn shank periosteum cells can promote the proliferation and differentiation of deer antler stem cells, inhibit cell apoptosis, and reduce the cell apoptosis rate. Promoting the proliferation of the deer antler stem cells is helpful to increasing the deer antler weight. The verification of the main function of the IGFBP3 gene provides more accurate and effective data for molecular selection and breeding of high-yield caprine. Therefore, the inhibitor of the IGFBP3 gene and / or the inhibitor of IGFBP3 protein are expected to increase the deer antler weight, and have a wide application prospect.

The invention discloses application of myricetin in proliferation and differentiation of intestinal stem cells, and belongs to the field of organoid culture. After the organ-like model is treated by myricetin, the relative expression quantity of Lgr5 mRNA is remarkably increased, which indicates that the model can respond to intervention of myricetin, up-regulate expression of an intestinal stem cell marker Lgr5, and contribute to proliferation and tissue repair of intestinal stem cells. Immunofluorescence detection shows that the protein expression intensity of the myricetin group MUC2 is enhanced, which proves that the model can verify the promotion effect of myricetin on goblet cell differentiation and mucus secretion and improve the intestinal barrier function. QPCR detection proves that after myricetin treatment, the relative expression quantity of mRNA closely connected with related genes ZO-1, Occludin and Claudin-1 is remarkably increased, which indicates that the organ model can evaluate the protection and strengthening effects of myricetin on intestinal epithelium close connection, and has potential application value in treatment of intestinal barrier injury related diseases.

The present invention generally relates to the provision of a conditioned medium comprising magnetically-induced cell secretome, the methods of producing the same, and a system for the production of said conditioned medium. More particularly, the present invention provides an improved method of producing a conditioned medium comprising cell secretome induced by a directionally-specific pulsing electromagnetic field (PEMF), wherein the conditioned medium of the present invention is capable of enhancing proliferation, differentiation, survival or senescence of recipient cells. Also provided are a system for the production of said PEMF-conditioned medium and the improved conditioned medium thereof, suitable for use in medical and commercial applications.

This invention relates to the field of biomedical technology and provides the application of ginsenoside Rg2 in the preparation of drugs for treating multiple sclerosis (MS). Ginsenoside Rg2 exerts its therapeutic effect on MS through the following mechanisms: reducing the expression levels of pro-inflammatory factors, promoting regulatory T cell differentiation, and inhibiting the infiltration of immune cells into the central nervous system; inhibiting NLRP3 signaling pathway activation and reducing neuronal pyroptosis; preventing the immune system from destroying myelin and reducing the ratio of demyelinated area to white matter area; upregulating oligodendrocyte transcription factor expression, promoting oligodendrocyte precursor cell proliferation and differentiation, and promoting myelin regeneration; and inhibiting microglia activation. This invention solves the problems of limited efficacy, significant side effects, only delaying progression, and limited administration methods of existing drugs.

The application discloses a skeletal muscle repair method and system based on muscle satellite cell regulation, and performs proliferation differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation map; a synergistic repair time window is determined according to the map, a difference response deviation correction parameter is extracted to form an activity correction factor, repair intensity correction is performed on the synergistic repair time window to generate a self-adaptive repair window; the self-adaptive repair window is subjected to exercise prescription adaptation and satellite cell activation regulation pathways are identified, an activation delay time is obtained through activation efficiency mapping, a response level is generated according to the activation delay time to form a layered repair configuration; a preferred activation mode is determined through activation delay evaluation on the layered repair configuration, dynamic activation characteristics are formed by extracting data of the best activation time window, skeletal muscle repair execution instructions are output in combination with an exercise prescription parameter table, and satellite cell repair state monitoring and dynamic synergistic adaptation of training scheme parameters are realized.

The present invention provides a composition for promoting proliferation, differentiation and maturation of neural stem cells, promoting activation of small glial cells, and promoting activation of astrocytes. It is found that a composition comprising lactic acid bacteria (in particular enterococcus faecium), clostridium butyricum (in particular clostridium butyricum), and saccharifying bacteria (in particular bacillus subtilis) has an effect of increasing the number of neural stem cells in a resting period, an effect of promoting differentiation and maturation of neural stem cells into nerve cells, an effect of promoting proliferation of neural stem cells, and an effect of promoting proliferation of neural stem cells. The present invention relates to an effect of promoting maturation of immature nerve cells, an effect of promoting activation of small glial cells, an effect of promoting activation of astrocytes, an effect of promoting generation of adult nerves, an effect of maintaining brain function homeostasis, and an effect of maintaining and / or improving memory and / or learning ability.

This invention provides a casein-derived 3D printing bio-ink, a bio-scaffold, and a method for preparing cell-cultured meat. The bio-ink is prepared from the following raw materials in parts by weight: gelatin: 15.6 parts; sodium alginate: 2.6 parts; casein: 4.2 parts; sodium hydroxide deionized water solution at pH=8: 52.5 parts; sterile 1×PBS buffer solution: 87.5 parts. Using this ink, a bio-scaffold is obtained through extrusion 3D printing and calcium chloride cross-linking. C2C12 cells are seeded onto the scaffold, and cell-cultured meat is obtained through proliferation-differentiation culture. This invention solves the problems of existing 3D printing bio-inks, such as limited performance, poor component compatibility, insufficient mechanical stability of bio-scaffold materials, and lack of nutritional components. The prepared bio-ink exhibits excellent mechanical properties and printing compatibility, providing key technical support for the industrial-scale preparation of cell-cultured meat.