124 results about "Proliferation differentiation" patented technology

The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and / or the generation of one or more tissue constructs from cell and / or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and / or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.

The invention provides compounds and methods for inhibition of kinases, more specifically IGF 1 R kinases. The invention also provides compounds and methods for inhibition of wildtype Abl. The invention provides compounds for modulating protein kinase enzymatic activity for modulating cellular activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. Compounds of the invention inhibit, regulate and / or modulate kinase receptor signal transduction pathways related to the changes in cellular activities as mentioned above, and the invention includes compositions which contain these compounds, and methods of using them to treat kinase-dependent diseases and conditions. A compound of formula (I), or a pharmaceutically acceptable salt, hydrate, or prodrug thereof, wherein, V is NR1R1a, or O—R1, wherein X is H, halo, C1-C6 alkyl, NO2, mono-, di-, or tri-halo substituted methyl, NR13R,14. C(O)O—C1-C6 alkyl, or N(R13)—C(O)—C1-C6 alkyl; Y is H, halo, OH, C1-C6 alkyl, C0-C6alkyl-NR,15R16, NR15R,6, C1-C6 alkoxy, —N(R13)—(CH2)n-NR15R16, —C(O)O—C1-C6 alkyl, —O—(CH2)n—NR15R16, —C(O)—C1-C6 alkyl, —C0-C6-alkyl-R21, —O—R21, —C(O)—R21, —O—(CH2)n—R21, —C(O)—NR13R14, —C(O)—N(R13)-aryl, —C(O)—N(R13)(CH2)n—NR15R16, —C(O)—N(R13)—(CH2)n-aryl —C(O)—N(R13)—(CH2)n-heterocyclyl; or X and Y together with the atoms to which they are attached form a 4-7 membered heterocyclyl or heteroaryl group containing one or two heteroatoms independently selected from O, N, and S. Z is H, NR2R3, —S—R2a, or —O—R2a

The present invention provides compounds for modulating protein kinase enzymatic activity for modulating cellu-lar activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. Even more specifically, the invention provides compounds for modulating c-Kit kinase activity and methods of treating diseases mediated by c-Kit activity utilizing the compounds and pharmaceutical compositions thereof.

The invention discloses methods of proliferation and differentiation of multipotent neural stem cells. Also provided are methods of making cDNA libraries and methods of screening biological agents which affect proliferation differentiation survival phenotype or function of CNS cells.

Disclosed herein is a composition for the treatment of articular cartilage damage or loss or defect. The composition for the treatment of articular cartilage injury of the present invention includes (i) cellular components separated, proliferated, and / or differentiated from the umbilical cord blood, (ii) a culture medium; and (iii) a biocompatible polymer. The composition has very superior ability of proliferation and differentiation and easier to be collected and acquired.

The invention discloses a constructing method of stem cell breeding and differentiation microenvironment in vitro in the stem cell tissue engineering domain, which comprises the following steps: adopting sodium alginate and polylysine as material to prepare APA microencapsulation ES cell; adding microencapsulation ES cell in the ES growing culture medium; stewing at 37 Deg C in the culture box with 5% CO2; changing culture medium every 2-3d to obtain the product. The invention simplifies the operation to generate high-density and large scale augmentation, which can inhibit or delay the oriental differentiation for ES cell.

The present invention provides compounds for modulating protein kinase enzymatic activity for modulating cellular activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. More specifically, the invention provides quinazolines and quinolines which inhibit, regulate, and / or modulate kinase receptor, particularly c-Met, KDF, c-Kit, flt-3 and flt-4, signal transduction pathways related to the changes in cellular activities as mentioned above, compositions which contain these compounds, and methods of using them to treat kinase-dependent diseases and conditions. The present invention also provides methods for making compounds as mentioned above, and compositions which contain these compounds.

The invention discloses a hormone-free rapid propagation organic production method of anoectochilus formosanus germchits. The method comprises the following steps of: step 1, sterilely germinating seeds, including disinfecting anoectochilus formosanus capsules and taking out the seeds, and inoculating the taken seeds on a culture medium I to be cultured until the seeds are germinated to protocorms; step 2, carrying out proliferation differentiation on the protocorms, including transferring the protocormss formed from seed germination to a culture medium II to have proliferation differentiation cultivation; and step 3, growing roots from strong seedlings, including transferring the protocorms to a culture medium III to have strong seedling root growth cultivation when the differentiation buddings of the protocorms grow to 1.5-2.5cm, and carrying out seeding hardening and transplantation when the seedlings grow to 5-6cm high and the roots are 2-3cm. According to the method, a technical route of seed, protocorm and germchit is adopted, and no any hormone is added into the culture mediums used during the cultivation process, so that on the basis that the rapid germination of the anoectochilus formosanus seeds is ensured, the purpose that the produced germchits have no residual exceeding is ensured, and germchits of high quality are provided for producing organic products of anoectochilus formosanus.

The invention discloses a bionic tissue engineering scaffold containing a micropore and nanofiber composite structure and a preparation method thereof. The bionic tissue engineering scaffold comprisesmicropores and a nanofiber network structure. The micropores have diameters of 20-200 micrometers, the diameter of the nanofiber network structure is 10-2000nm and the length of the nanofiber networkstructure is 1-100 micrometers. The bionic tissue engineering scaffold fully simulates the structural features of a natural extracellular matrix, the micropores provide conditions for cell growth into the scaffold and high porosity is conducive to permeation and diffusion of oxygen and nutrients. The network structure of the nanometer fiber network bionic extracellular matrix can promote cell adhesion, growth, proliferation, differentiation and migration. The preparation method well solves the problems of the existing traditional scaffold preparation method and has the potential of becoming anovel tissue engineering scaffold preparation technology.

The invention discloses a method for constructing tissue engineering islet by adipose derived mesenchymal stem cells and the obtained tissue engineering islet. The method employs adipose derived mesenchymal stem cells with extensive sources to perform culture on islet acellular matrix, so that the acellular matrix can provide a three-dimensional space suitable for growth to adipose derived mesenchymal stem cells so as to promote cell adhesion, proliferation and differentiation, and formation of a three-dimensional structure engineering islet tissue able to stably secrete insulin. The method realizes gathering of insulin secreting cells, enhances cell utilization and is expected to promote the effect of repairing islet injury by tissue engineering method.

The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells.

In a method and apparatus for analyzing multivariable data sets, a general computerized platform is provided for evaluating the relationship between large number of measurements of sets of variables characterizing components of complex states of a system under induced stimulation or controlled conditions. The linked responses of variables and their temporal relations tell about the network of interactions and their hierarchy. Processing of data sets by a simple neural network gives a matrix of weight parameters, that allow to identify fingerprints of complex states characterized by patterns of measured variable and estimate the interactions between the components characterized by the measured variables. The results are provided numerically and by color-coded presentation indicating dominating relations between variables and strongly responding variables. When applied to dynamic responses of a system, the analysis can construct a schematic hierarchical architecture of the network of interaction between the components of the studied system. Applications in biology include analysis of measurements characterizing responses of molecular components in cells under changes induced by stimuli (e.g. drugs, growth factors, hormones, mutations or forced expression of a proteins), and identification of complex cellular states (e.g. proliferation, differentiation, transformation, starvation, necrosis, apoptosis, and the time dependencies of the above effects).

The invention provides a targeting nucleic acid drug as well as a preparation method and an application thereof. The targeting nucleic acid drug comprises an amino lipid-hyaluronic acid conjugate, a cationic liposome and a complex containing protamine, chondroitin sulfate and a nucleic acid drug, the amino lipid part of the amino lipid-hyaluronic acid conjugate is combined with the cationic liposome, and the cationic liposome wraps the complex. The targeting nucleic acid drug inhibits self-renewal and migration invasion of cancer cells by specifically blocking a Hedgehog signal transduction pathway of cell self-proliferation and differentiation, induces cell apoptosis, and can effectively inhibit recurrence of tumors.

The invention relates to the technical field of cryopreservation solutions, in particular to a human umbilical cord mesenchymal stem cell injection cryopreservation solution and a preparation method thereof. Preparation raw materials of the human umbilical cord mesenchymal stem cell injection cryopreservation liquid at least comprise the following components: human serum albumin, dimethyl sulfoxide, a compound electrolyte injection, a sodium chloride injection and a glucose injection. The human umbilical cord mesenchymal stem cell injection cryopreservation liquid provided by the invention is convenient in material taking, does not violate ethical problems, has small toxic and side effects on cells, is suitable for clinical use, obviously improves the safety and biocompatibility of cell reinfusion to a human body, and can improve the motility and proliferation and differentiation capabilities of umbilical cord mesenchymal stem cells on the premise of reducing the use amount of DMSO (Dimethylsulfoxide); after resuscitation, direct reinfusion is carried out, centrifugal washing is not needed, and damage to cells is reduced.

The present invention provides novel substituted pyrazolone compounds, pharmaceutical acceptable salts and formulations thereof useful in modulating the protein tyrosine kinase activity, and in modulating cellular activities such as proliferation, differentiation, apoptosis, migration and invasion. The invention also provides pharmaceutically acceptable compositions comprising such compounds and methods of using the compositions in the treatment of hyperproliferative disorders in mammals, especially humans.