57 results about "Proliferation differentiation" patented technology

The invention discloses a bionic nanofiber-hydrogel composite biological scaffold as well as a preparation method and application thereof. The bionic nanofiber-hydrogel composite biological scaffold is a composite scaffold for simulating a gray-white matter partition structure of a spinal cord tissue, and comprises bionic nanofibers and a bionic hydrogel material, wherein the bionic nanofibers are arranged in an oriented manner and simulate a white matter ordered structure around the spinal cord, and the bionic hydrogel material is combined with the bionic nanofibers and simulates gray matter in the center of the spinal cord. According to the bionic nanofiber-hydrogel composite biological scaffold, the biological activity of loaded stem cells or adult cells can be improved, the problem that a large number of transplanted exogenous cells die due to unbalanced oxidative stress generated after tissue damage can be solved, and it is guaranteed that the transplanted cells reside in a damaged area and play a role; therefore, the spinal cord microenvironment is improved, proliferation and differentiation of endogenous neural stem cells are promoted, functional recovery after spinal cord injury is finally promoted, and great potential is achieved in spinal cord injury treatment.

The invention belongs to the technical field of hydrogel, and relates to piezoelectric / conductive integrated hydrogel for jaw defect repair, which is prepared from methylacryloyl protein, zwitterionic monomer and titanate piezoelectric particles through ultraviolet crosslinking under the action of a crosslinking agent and a photoinitiator, the hydrogel comprises, by mass, 1.0%-10% of methacrylated protein, 1.0%-10% of zwitterionic monomers, 1.0%-10% of titanate piezoelectric particles and 0.1%-5% of an initiator, and the volume fraction of an accelerant is 0.05%-1.0%. The hydrogel disclosed by the invention has excellent mechanical strength and certain flexibility, can realize piezoelectric and conductive functions of the hydrogel, can effectively promote proliferation, differentiation and mineralization of bone cells under the action of an electric field, accelerates bone tissue repair, and has a more remarkable bone regeneration effect compared with a non-electrical stimulation material in the prior art.

The invention relates to the technical field of biological medicine, in particular to a drug screening model construction method based on airway organs, a model and application. The construction method of the drug screening model of the airway organoid comprises the steps of obtaining 2D airway epithelial cells, culturing the airway organoid, establishing an airway organoid inflammation model and / or establishing an airway organoid mucus secretion / goblet cell metaplasia model. Wherein the model is established according to the culture mode of the airway organ in cooperation with a specific stimulant for proliferation and differentiation. Compared with a traditional animal model, the organ-like model is low in cost and short in construction period, can better simulate the pathological process, can realize airway organ-like culture and establishment of an inflammation model and a mucus secretion / goblet cell metaplasia model at the same time, and has a good application prospect in drug detection of asthma and COPD diseases.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention provides a method for detecting genes related to early growth traits of Inner Mongolia down producing goats based on copy number variation (CNV). The method comprises the following steps: acquiring whole genome re-sequencing data of 461 Inner Mongolia down producing goats; the method comprises the following steps: detecting autosomal CNV by using CNV native software, and screening out 26,003 non-redundant CNV which covers 1, 015.4 Mb (38.97%) of an autosomal genome; gWAS analysis is carried out on the basis of CNV, 11 CNVs obviously related to early growth traits (birth weight CSZ, weaning weight DRZ, daily gain ADG before weaning and 12-month-old weight ZWT) are identified, and two CNVs influence ADG and DRZ at the same time; seven key candidate genes (ZN845, SOX15, FGF11, GPS2, DVL2, SPRY4 and STAT2) are analyzed and identified through bioinformatics, and GO and KEGG function enrichment analysis is utilized to find that the genes participate in biological pathways such as cell proliferation, differentiation and protein phosphorylation. The invention provides a theoretical basis for molecular marker-assisted breeding of Inner Mongolia down producing goats, and has important scientific value and application prospect.

The application discloses a culture medium for tissue culture of Limosella australis, which comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium. The primary culture medium is MS+6BA 0.4-0.6 mg / L+NAA 0.04-0.06 mg / L+AC 0.8-1.2 mg / L+2,4-D 0.04-0.06 mg / L. The proliferation and differentiation culture medium is modified MS+6BA 0.09-0.11 mg / L+NAA 0.04-0.06 mg / L+AC 1.5-2.5 mg / L. The rooting culture medium is 1 / 2MS+IAA 0.09-0.11 mg / L+AC 2.5-3.5 mg / L. The technical scheme has the highest callus induction rate of 86.7%, the highest differentiation rate of 86.7% and the highest rooting rate of 93.3%.

The invention discloses a skeletal muscle repair method and system based on muscle satellite cell regulation and control. The method comprises the following steps: performing proliferation and differentiation correlation analysis on satellite cell steady state data and aerobic exercise intensity data to establish a proliferation regulation and control map; determining a collaborative repair time window according to the atlas, extracting difference response deviation correction parameters to form an activity correction factor, and performing repair intensity correction on the collaborative repair time window to generate a self-adaptive repair window; carrying out exercise prescription adaptation on the self-adaptive repair window, identifying a satellite cell activation regulation and control channel, obtaining activation delay time through activation efficiency mapping, and delimiting a response level according to the activation delay time to generate layered repair configuration; activation delay evaluation is conducted on layered repair configuration to determine a preferred activation mode, dynamic activation characteristics are extracted based on optimal activation time window data to form an intervention execution sequence, a skeletal muscle repair execution instruction is output in combination with an exercise prescription parameter table, and dynamic collaborative adaptation of satellite cell repair state monitoring and training scheme parameters is achieved.

The present application relates to a method for inducing pluripotent cells to proliferate and / or differentiate into megakaryocyte progenitor cells (MKPs), comprising adding a human platelet lysate (hPL) and an HDAC inhibitor to an MKP differentiation medium. The present application also provides a culture medium used in the method, and a composition comprising the culture medium.

The invention provides a cleaning and preserving fluid for fetal brain tissues and a preserving method thereof, and belongs to the technical field of tissue preservation. The cleaning and preserving fluid comprises trehalose, nerve growth factors, a GlutaMAX supplement, Y27632, S-nitrosoglutathione, tert-butylhydroquinone, a Versene solution and polyvinylpyrrolidone, compared with a traditional tissue preserving fluid, the cleaning and preserving fluid not only can improve the purity of neural stem cells, but also can maintain the activity and the yield of the neural stem cells in fetal brain tissue, and the cleaning and preserving fluid has the advantages that the cleaning and preserving fluid is simple in structure and convenient to use. The structural integrity of the cells is guaranteed, a high-quality sample is provided for subsequent research on proliferation, differentiation and the like of the neural stem cells, and the method has a wide application prospect.

The invention discloses a cell culture medium supplement as well as a preparation method and application thereof, and relates to the technical field of cell biology. The supplement comprises DL-alpha-tocopheryl acetate, linolenic acid, ethanolamine, L-carnitine hydrochloride, 3, 3 ', 5-triiodoL-sodium thyrosinate, and serum albumin. As a cell culture additive with definite chemical components and simple composition, the cell culture additive can replace a traditional culture system with unknown chemical components or too complex composition, and is used for supporting proliferation, differentiation and characterization analysis of cells. The culture medium additive is clear in chemical component, simple to prepare, stable in effect and relatively low in cost, can promote in-vitro proliferation and oxidation resistance of cells including human embryonic stem cells and mesenchymal stem cells, and has important application value in actual production.

The present invention discloses a 3D printed bionic meniscus scaffold and a preparation method thereof. It belongs to the field of medical prosthesis technology. The meniscus scaffold prepared by the present invention is composed of a layer of annular fiber layer and two layers of rotating grid-shaped fiber layers stacked from bottom to top, and has good mechanical properties. In biological tissue engineering technology, it can effectively avoid the adverse effects caused by the formation of a well-shaped barrel structure, which is conducive to the growth of cells in all directions, and provides a framework and metabolic site for cell adhesion, proliferation, differentiation and the final formation of new tissues. The present invention also provides a preparation method for the above-mentioned meniscus, which effectively simulates the internal collagen fiber structure of the meniscus through 3D printing technology, and has more excellent biomechanical properties, biocompatibility and the ability to promote cell secretion of extracellular matrix.

The present application relates to a method for inducing proliferation and / or differentiation of pluripotent cells into megakaryocyte progenitor cells (MKP), comprising adding human platelet lysate (hPL) and an HDAC inhibitor to an MKP differentiation medium. The invention also provides a culture medium used by the method and a composition containing the culture medium.

The invention relates to the technical field of biological medicines, and particularly discloses an application composition of stem cell exosomes in promoting tissue repair, which comprises the stem cell exosomes selected from induced pluripotent stem cells, mesenchymal stem cells, embryonic stem cells, hematopoietic stem cells or neural stem cells and used for promoting tissue repair and cell regeneration, and the stem cell exosomes selected from the induced pluripotent stem cells, the mesenchymal stem cells, the embryonic stem cells, the hematopoietic stem cells and the neural stem cells. The tissue repair promoting factor is selected from an epidermal growth factor, a transforming growth factor beta, a platelet-derived growth factor or a combination thereof, and is used for optimizing cell proliferation, differentiation and wound healing; the biodegradable carrier is selected from hydrogel, lipidosome, polymer micelle, nano particles or natural plant extract; by optimizing the transmission relation between the biodegradable carrier and the stem cell exosome and combining with the cell proliferation model, different doses and delivery modes can be flexibly regulated and controlled, the treatment scheme is further optimized, and the effect of the treatment scheme is accurately predicted. Due to the design, the common problems of unstable effect, long treatment period and the like in the traditional stem cell application are solved.

The invention belongs to the technical field of medical biology, and particularly relates to application of porcine cumulus cell miR-31 in improvement of maturation quality of oocytes, and the nucleotide sequence of the porcine cumulus cell miR-31 is shown as SEQ ID NO.1. The invention also relates to a preparation method of the porcine cumulus cell miR-31. The miR-31 can improve the cumulus expansion level, improve the oocyte quality, enhance the mitochondrial function and reduce oxidative stress, target MAPK8 to participate in a JNK channel, inhibit the apoptosis level of the oocyte and remarkably improve the in-vitro oocyte quality. The miR-31 can be used as a potential biomarker for maturation of oocytes. The results provide a theoretical basis for further discussing the effect of miRNA participating in proliferation and differentiation of animal reproduction related cells and the action mechanism of miRNA on follicle development and oocyte maturation.

The application discloses application of a polysaccharide wrapping film in preparation of a cell culture product and belongs to the technical field of cell culture food. The application specifically forms the polysaccharide wrapping film on the periphery of a cell inoculation support after the cell inoculation support, can relieve the phenomenon that cells fall off from the support during culture, and further guarantees effective proliferation and differentiation of muscle stem cells and / or fat stem cells on the support, thereby providing a new solution approach for efficient and standardized production of the cell culture product.

The utility model relates to the technical field of medical instruments, and discloses a full-porous cervical vertebra fusion cage which comprises a machining area and a porous area. The machining area is provided with a threaded hole for leading in a surgical instrument; the porous area comprises an upper surface, a lower surface, a front surface, a rear surface and two side surfaces; and the upper surface and the lower surface are provided with anti-skid surfaces. The threaded hole in the machining area is used for being matched with a surgical instrument matched with the threaded hole, and surgical implantation is facilitated. The porous area provides more growth space for cells, adhesion, migration, proliferation and differentiation of the cells are facilitated, and finally the bone ingrowth effect is achieved. The full-porous cervical vertebra fusion cage has the mechanical property matched with the human body structure and good biocompatibility, can form a tight bonding interface with the human body tissue, improves the combination stability of the fusion cage and the human body tissue, has the mechanical property matched with the human body tissue, and can be applied to clinical treatment of cervical degenerative diseases.

The present invention relates to 1,2-dicarboxamide compounds of Formula (I); pharmaceutically acceptable salts, pharmaceutically acceptable stereoisomers, N-oxides or combination thereof, wherein 'X', 'Y', 'Z', ring A, ring B, R1, R2, R3, R4, R5, R6, 'm' and 'n' are as defined herein. The present invention also relates to 1,2-dicarboxamide compounds that modulate cellular activities like proliferation, differentiation, adhesion, migration and apoptosis by modulating protein kinase enzymatic activity. In particular the invention relates to compounds which inhibit, regulate, and / or modulate tyrosine kinases such as FLT1 (VEGFR1), FLT4 (VEGFR3), KDR (VEGFR2), MET (C-Met), MERTK (c-Mer), RET, AXL, TEK (TIE 2) and EGFR. The present disclosure relates to 1,2-dicarboxamide compounds for use in modulating kinase enzymatic activity and accordingly modulating kinase-dependent associated diseases and conditions such as cancers like Thyroid carcinoma, Ovarian carcinoma, Pancreatic carcinoma, Prostatic carcinoma, Renal cell carcinoma, Hepatocellular carcinoma, Breast carcinoma, Colorectal carcinoma, Oral squamous cell carcinoma, Colorectal, Lung adenocarcinoma or Endometrial cancer.

The application belongs to the field of biomedical science and neurology, and particularly relates to application of CHCHD2 gene and a protein coded by the CHCHD2 gene in preparation of a product for promoting proliferation and differentiation of neural stem cells. The application proves that the CHCHD2 gene and the protein coded by the CHCHD2 gene are highly expressed in in-vivo neural development and in-vitro neural differentiation process; and that knocking down or over-expressing the CHCHD2 gene or the protein coded by the CHCHD2 gene has a certain regulating effect on proliferation and differentiation of neural stem cells. The application provides a new scheme for preparation of a product for promoting proliferation and differentiation of neural stem cells, and provides a new target for drug research and development of neural regeneration and repair treatment of central nervous system development deficiency diseases, neurodegenerative diseases, cerebrovascular diseases, craniocerebral and spinal cord injuries.

According to the method for differentiating the SH-SY5Y cells into the neuron-like cells, rapid proliferation of the cells is facilitated in a DMEM culture medium containing 10% of serum, a differentiation reagent EC23 is used when differentiation begins, a DMEM / F12 culture medium with the concentration of 5% of serum is replaced, cell differentiation is facilitated, and finally, a serum-free culture medium NBA containing a differentiation reagent BDNF is used for differentiating the cells into the neuron-like cells. Compared with the prior art, the neuron-like cells with high SH-SY5Y differentiation efficiency can be rapidly obtained, a network is formed between the improved neuron-like cells, the survival rate of the cells is also greatly improved, and the neuron-like cells can be better used for research of subsequent experiments.

This application proposes a method for three-dimensional cell culture using an injectable thermosensitive hydrogel. Cells are mixed with a thermosensitive hydrogel precursor solution and incubated at 34-37°C for 1-10 minutes, followed by the addition of cell culture medium. After culture, a vitamin B6 derivative is added for degradation, yielding a cell suspension. The cultured cells are then separated by centrifugation. Using a hydrogel with a rapid sol-gel phase transition provides cells with a three-dimensional support environment close to the in vivo environment, enabling cells to grow, proliferate, differentiate, and interact with other cells and the matrix under similar physiological conditions, achieving high-quality in vitro cell culture. Specifically, the three-dimensional structure is less affected by temperature during culture, which is beneficial for stable cell culture. After culture, the gel structure degrades rapidly and completely, allowing for rapid cell recovery; this is a high-quality cell culture method.

The invention provides an antigen peptide of a Thr255 O-GlcNAc glycosylation site of a targeted PGK1 protein, an antibody and application of the antigen peptide and the antibody. The antigen peptide has immunogenicity of a Thr255 O-GlcNAc glycosylation site of a targeted PGK1 protein, and the amino acid sequence of the antigen peptide is as shown in any one of SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4. The antigen peptide provided by the invention is helpful for researching the important effect of PGK1 in cell energy balance, and also provides a potential action target for the key effect possibly played in the biological processes of tissue repair, stem cell differentiation, damage repair and the like; and how to regulate and control glycosylation modification of PGK1 is further explored, so that proliferation, differentiation and tissue repair capacities of stem cells are enhanced, and a potential application prospect is provided for the field of regenerative medicine.

The present invention provides a soft tissue repair material and a preparation method thereof, comprising the following steps: soaking the extracellular matrix in a composite amino acid phosphate buffer solution containing L-arginine, L-asparagine, and L-isoleucine for protecting the structure and activity before decellularization; washing after soaking, and performing decellularization treatment with a surfactant to obtain a decellularized tissue; after washing, soaking the decellularized tissue in an MES buffer solution, and then soaking it in an MES buffer solution containing EDC and NHS for fixation, and obtaining a tissue with a fixed structure after washing; placing the tissue with a fixed structure in an NaOH solution for virus inactivation, and obtaining a soft tissue repair material after washing. The method of the present invention can maximize the retention of the structural integrity and active components of the extracellular matrix in the decellularized extracellular matrix, facilitate the influence of the extracellular matrix on cell proliferation and differentiation, and be better used for soft tissue repair.

The application belongs to the field of tissue repair porous material research, and discloses a 3D printing cell culture chip for high-throughput structure screening and a preparation method and application thereof. The chip is formed by 3D printing and comprises a cell culture block, an anti-adhesion belt, a cell isolation groove and a substrate. The cell culture block is composed of a structural matrix of unit structures and / or parallel groups thereof, at least one of which has a gradient change in curvature, porosity, pore size and connected pore size. The isolation groove contains the anti-adhesion belt. The 3D printing cell culture chip for high-throughput structure screening can realize the detection and analysis of the adhesion, proliferation and differentiation of various cells on the high-throughput structural matrix under the same time and the same culture environment, and realizes rapid structure screening. The chip is integrated with a variety of non-cytotoxic materials combined with 3D printing technology, and is applied to the structure optimization research work of various tissue repair materials, thereby improving the work efficiency of structure screening.

The invention discloses a culture method of cornu cervi pantotrichum stem cells, and belongs to the technical field of tissue engineering. The culture method comprises the following steps: taking antler stem periosteum tissue, refrigerating and transporting the antler stem periosteum tissue through a special transportation preserving fluid, cutting, cleaning and centrifuging to obtain tissue precipitate; resuspending the precipitate by using an optimized serum-free culture medium, inoculating to a culture dish pre-coated with gelatin according to a specific density, standing and culturing, and removing non-adherent cells; after trypsinization and centrifugation, cells are resuspended by using a sorting buffer solution, and a cell population of a specific surface marker is screened by using a flow cytometry sorter, so that the antler stem cells are obtained; and finally, carrying out passage amplification under a low-oxygen condition to obtain the pilose antler stem cells after passage amplification. By optimizing a transportation and preservation system, a culture environment and a sorting process, the purity, the activity and the proliferation and differentiation capacity of the pilose antler stem cells are effectively improved, the process standardization degree is high, and reliable technical support is provided for application of the pilose antler stem cells in the fields of tissue engineering, regenerative medicine and the like.

The invention discloses application of combination of CD40L and cytokines in promotion of in-vivo or in-vitro proliferation and / or differentiation of HIS mouse B cells. The invention also discloses a construction method of the fully human monoclonal antibody development platform. The construction method comprises the following steps: firstly, taking an HIS mouse immune antigen as a first signal; and giving CD40L to the HIS mouse, taking a cell factor as a second signal, taking the spleen, and sorting B cells which are specifically combined with the antigen, so that the antigen-specific antibody can be screened out. According to the invention, an effective second signal combination is clearly screened out in vitro, and B cells are promoted to differentiate towards plasma mother cells. In vivo, a scheme of'antigen + CpG sensitization-> 24 hours-> second signal intravenous injection 'is adopted, so that the proportion and total number of antigen-specific B cells are remarkably improved, the antigen-specific B cells are promoted to be converted from TR / Naive to Bmem (CD38-), and meanwhile, the antigen-specific antibody titer is improved.

The invention provides a method for drawing a sepsis immune cell dynamic change map and application, and belongs to the technical field of sepsis treatment. On the basis of the scRNA-seq technology, the immune library is constructed, the immune microenvironment is more comprehensively analyzed by adopting the scVDJ-seq sequencing technology, the cloning source of a single cell can be accurately monitored, the proliferation, differentiation and mutation paths of the cloned cell can be reconstructed, and the microenvironment with the immune function change can be more accurately obtained. The map drawn by the method can allow clinical workers to more accurately evaluate the immune state of the patient through related marker genes, so that dynamic change capture can guide the treatment opportunity of immune regulation of the sepsis patient in clinic, and accurate treatment of the sepsis immune function can be realized.

The invention relates to a particle acellular matrix and a preparation method and application thereof.The particle acellular matrix comprises acellular matrix particles with gradient particle sizes and can be used for promoting skin wound healing, and the particle acellular matrix makes full contact with a complex wound surface and is degraded in a staged mode; a favorable microenvironment can be continuously provided for migration, growth, proliferation and differentiation of cells, so that wound healing is promoted.

The invention provides application of LncRNA as an ovarian cancer biomarker and a therapeutic target, and belongs to the field of biotechnology and medicine. The LncRNASLC8A1-AS1 is used as an ovarian cancer marker to positively regulate the occurrence and development of ovarian cancer, proliferation and differentiation of ovarian cancer cells can be inhibited by inhibiting the expression of the LncRNASLC8A1-AS1, an effective molecular marker is provided for diagnosis, disease assessment and prognosis of ovarian cancer, and a new target is also provided for treatment of ovarian cancer.

Provided in the present invention is a new 3D microtissue model based on tissue microenvironment reduction. Specifically, provided in the present invention is the use of a cytokine combination in the promotion of the growth of a tumor 3D micromicrotissue model, wherein the cytokine combination is selected from at least two of IL-6, Gremlin-1, FGF2, HGF and IGF-1. The combination of cytokines can promote the proliferation, differentiation and tissue formation of a tumor microtissue model to obtain more microtissue models with larger volumes, so that the growth microenvironment of a tumor can be reproduced in a more realistic manner, and a more comprehensive tool is provided for the subsequent research of disease mechanisms and the screening and development of drugs.

The invention relates to the technical field of biology, in particular to a method for detecting biological functions of mesenchymal stem cells. The invention provides a method for evaluating the functions of mesenchymal stem cells by using the combination of surface molecules CD155 and secretory factors IDO, which is different from the conventional detection method and is characterized in that the biological functions of the cells are rapidly judged by detecting the surface CD155 of the mesenchymal stem cells through flow cytometry and detecting the concentration of cytokines IDO through an ELISA (Enzyme-Linked Immuno Sorbent Assay) method. The biological functions refer to proliferation, differentiation, adhesion, anti-apoptosis, factor secretion and anti-inflammatory functions of the mesenchymal stem cells, and the method is rapid and accurate, has a wide application prospect and is worthy of popularization and application.