133 results about "Proliferation differentiation" patented technology

An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

The present invention provides compounds for modulating protein kinase enzymatic activity for modulating cellular activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. More specifically, the invention provides appropriately functionalized 5,6-fused bicyclics which inhibit, regulate and / or modulate kinase receptor, particularly c-Met, KDR, and flt-3, signal transduction pathways related to the changes in cellular activities as mentioned above, compositions which contain these compounds, and methods of using them to treat kinase-dependent diseases and conditions.

The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and / or the generation of one or more tissue constructs from cell and / or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and / or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.

The invention provides compounds and methods for inhibition of kinases, more specifically IGF 1 R kinases. The invention also provides compounds and methods for inhibition of wildtype Abl. The invention provides compounds for modulating protein kinase enzymatic activity for modulating cellular activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. Compounds of the invention inhibit, regulate and / or modulate kinase receptor signal transduction pathways related to the changes in cellular activities as mentioned above, and the invention includes compositions which contain these compounds, and methods of using them to treat kinase-dependent diseases and conditions. A compound of formula (I), or a pharmaceutically acceptable salt, hydrate, or prodrug thereof, wherein, V is NR1R1a, or O—R1, wherein X is H, halo, C1-C6 alkyl, NO2, mono-, di-, or tri-halo substituted methyl, NR13R,14. C(O)O—C1-C6 alkyl, or N(R13)—C(O)—C1-C6 alkyl; Y is H, halo, OH, C1-C6 alkyl, C0-C6alkyl-NR,15R16, NR15R,6, C1-C6 alkoxy, —N(R13)—(CH2)n-NR15R16, —C(O)O—C1-C6 alkyl, —O—(CH2)n—NR15R16, —C(O)—C1-C6 alkyl, —C0-C6-alkyl-R21, —O—R21, —C(O)—R21, —O—(CH2)n—R21, —C(O)—NR13R14, —C(O)—N(R13)-aryl, —C(O)—N(R13)(CH2)n—NR15R16, —C(O)—N(R13)—(CH2)n-aryl —C(O)—N(R13)—(CH2)n-heterocyclyl; or X and Y together with the atoms to which they are attached form a 4-7 membered heterocyclyl or heteroaryl group containing one or two heteroatoms independently selected from O, N, and S. Z is H, NR2R3, —S—R2a, or —O—R2a

The present invention provides compounds for modulating protein kinase enzymatic activity for modulating cellu-lar activities such as proliferation, differentiation, programmed cell death, migration and chemoinvasion. Even more specifically, the invention provides compounds for modulating c-Kit kinase activity and methods of treating diseases mediated by c-Kit activity utilizing the compounds and pharmaceutical compositions thereof.

The invention relates to the technical field of biological medicinal materials, and particularly relates to a 3D printed porous metal with a bionic three-dimensional (3D) micro-scaffold and a preparation method of the 3D printed porous metal. The bionic 3D micro-scaffold is constructed in each hole of a metal scaffold, and an excellent environment is provided to proliferation and differentiation of cells; the problems in the prior art that cells can only climb and grow on the two-dimensional (2D) space of a pore wall of the metal scaffold due to too large pore, and 3D growth in the whole hole cannot be realized can be solved. The bionic 3D micro-scaffold provided by the invention is possibly used for completely curing patients with bone tissue defect caused by diseases, accidents and other reasons, can be used as a novel interbody fusion cage, is suitable for spinal fusion surgeries, brings hopes for more and more patients, and has an important clinical application value.

The invention discloses methods of proliferation and differentiation of multipotent neural stem cells. Also provided are methods of making cDNA libraries and methods of screening biological agents which affect proliferation differentiation survival phenotype or function of CNS cells.

The invention discloses methods of proliferation and differentiation of multipotent neural stem cells. Also provided are methods of making cDNA libraries and methods of screening biological agents which affect proliferation differentiation survival phenotype or function of CNS cells.

Disclosed herein is a composition for the treatment of articular cartilage damage or loss or defect. The composition for the treatment of articular cartilage injury of the present invention includes (i) cellular components separated, proliferated, and / or differentiated from the umbilical cord blood, (ii) a culture medium; and (iii) a biocompatible polymer. The composition has very superior ability of proliferation and differentiation and easier to be collected and acquired.

The invention discloses a constructing method of stem cell breeding and differentiation microenvironment in vitro in the stem cell tissue engineering domain, which comprises the following steps: adopting sodium alginate and polylysine as material to prepare APA microencapsulation ES cell; adding microencapsulation ES cell in the ES growing culture medium; stewing at 37 Deg C in the culture box with 5% CO2; changing culture medium every 2-3d to obtain the product. The invention simplifies the operation to generate high-density and large scale augmentation, which can inhibit or delay the oriental differentiation for ES cell.

The invention relates to a stevia rebaudiana tissue culture propagation method and a culture medium of the method, and particularly relates to a tissue culture rapid propagation method for directly forming a bud by using a stevia rebaudiana leaf as an explant. The stevia rebaudiana tissue culture method comprises the following steps: (1), collecting the explant; (2), disinfecting and inoculating; (3), performing thickening induction-culturing; (4), budding and seedling; (5), transferring and grafting, and rooting; and (6), hardening and transplanting. According to the stevia rebaudiana tissue culture method provided by the invention, materials are easily available, a plurality of seed sources can be rapidly propagated, the explant tissue culture has no culture phase of callus cell proliferation and differentiation, thus the culture time is shortened, extremely high reproduction rate is achieved; the stevia rebaudiana tissue culture propagation method is easy to operate and is capable of realizing large-scale production; and the operation of researches such as genetic transformation is facilitated in a culture process. The culture medium provided by the invention ensures that the leaf is capable of reaching the expected culture target in each phase.

The invention discloses a recombinant human-like collagen protein-human original cell growth factor fusion protein and a preparation method and application thereof. The prepared fusion protein is provided with a special fibrous structure and good biological tissue compatibility, and can promote proliferation and differentiation of epidermis cells, endothelial cells and fibroblast cells. The preparation method comprises the following steps: obtaining encoded DNA sequence of the fusion protein, reconstructing and recombining fusion protein expressed by an expression vectors in escherichia coli, pichia pastoris or chinese hamster ovary cells. The fusion protein comprises a first zone and a second zone, wherein the first zone has at least 85% of sequence homology with human collagen protein and has the function of the human collagen protein, and the second zone has at least 85% of human original cell growth factor and has the function of the human original cell growth factor; connecting peptide is arranged between the first zone and the second zone; the general formula of the connecting peptide is (GGGS)n; preferably, the amino acid sequence of the fusion protein is SEQ ID NO. 15.

The invention discloses a hormone-free rapid propagation organic production method of anoectochilus formosanus germchits. The method comprises the following steps of: step 1, sterilely germinating seeds, including disinfecting anoectochilus formosanus capsules and taking out the seeds, and inoculating the taken seeds on a culture medium I to be cultured until the seeds are germinated to protocorms; step 2, carrying out proliferation differentiation on the protocorms, including transferring the protocormss formed from seed germination to a culture medium II to have proliferation differentiation cultivation; and step 3, growing roots from strong seedlings, including transferring the protocorms to a culture medium III to have strong seedling root growth cultivation when the differentiation buddings of the protocorms grow to 1.5-2.5cm, and carrying out seeding hardening and transplantation when the seedlings grow to 5-6cm high and the roots are 2-3cm. According to the method, a technical route of seed, protocorm and germchit is adopted, and no any hormone is added into the culture mediums used during the cultivation process, so that on the basis that the rapid germination of the anoectochilus formosanus seeds is ensured, the purpose that the produced germchits have no residual exceeding is ensured, and germchits of high quality are provided for producing organic products of anoectochilus formosanus.

The invention discloses a bionic tissue engineering scaffold containing a micropore and nanofiber composite structure and a preparation method thereof. The bionic tissue engineering scaffold comprisesmicropores and a nanofiber network structure. The micropores have diameters of 20-200 micrometers, the diameter of the nanofiber network structure is 10-2000nm and the length of the nanofiber networkstructure is 1-100 micrometers. The bionic tissue engineering scaffold fully simulates the structural features of a natural extracellular matrix, the micropores provide conditions for cell growth into the scaffold and high porosity is conducive to permeation and diffusion of oxygen and nutrients. The network structure of the nanometer fiber network bionic extracellular matrix can promote cell adhesion, growth, proliferation, differentiation and migration. The preparation method well solves the problems of the existing traditional scaffold preparation method and has the potential of becoming anovel tissue engineering scaffold preparation technology.

The invention relates to a separation, culture and induction differentiation method for precursor adipocyte in chicken muscle and belongs to the technical field of cytology. According to the method, hormone mixture 3-isobutyl-1-methylxanthine, dexamethasone and insulin are used, an induction differentiation system of external-source oleic acid and rosiglitazone is added, generation of oil drop ofprecursor adipocyte in the chicken muscle is induced, and an effective research means can be provided for the quality of poultry meat and particularly fat deposition in poultry muscle. By adopting themethod, intramuscular precursor adipocyte which is even in component and exuberant in proliferation and differentiation can be obtained, the effective induction differentiation system of adipocyte inthe chicken muscle is obtained, and the method has the advantages that the method is simple and convenient, easy to operate, low in cost, high in vigor and excellent in proliferation and differentiation capacity and can achieve acquirement of a large number of cells.

The invention discloses a method for constructing tissue engineering islet by adipose derived mesenchymal stem cells and the obtained tissue engineering islet. The method employs adipose derived mesenchymal stem cells with extensive sources to perform culture on islet acellular matrix, so that the acellular matrix can provide a three-dimensional space suitable for growth to adipose derived mesenchymal stem cells so as to promote cell adhesion, proliferation and differentiation, and formation of a three-dimensional structure engineering islet tissue able to stably secrete insulin. The method realizes gathering of insulin secreting cells, enhances cell utilization and is expected to promote the effect of repairing islet injury by tissue engineering method.

The invention provides a method for preparing polyelectrolyte composite coating. Disulfide bonds formed between sulfydryl of RGD specifically crosslink the polyelectrolyte composite coating, as a result, crosslinking of the polyelectrolyte composite coating is realized while RGD is introduced; and in addition, as the disulfide bonds are introduced, the biodegradable property of the final polyelectrolyte composite coating can be provided with in vivo responsiveness. The coating is suitable for surface treatment of titanium radical implants embedded in vivo, can promote adherence proliferation differentiation of bone precursors, can promote early development of ossification around the implants, can realize fast osseointegration between hard tissues and permanent implanted materials and facilitates long-term stability of implanted materials. Polyelectrolytes used in the invention all have fine biodegradability and biocompatibility, the degradation products of the polyelectrolytes can be absorbed by human bodies; and in addition, animal experiments indicate that the polyelectrolyte composite coating can promote osteogenesis of injured parts and can be constructed on the surface of medical pure titanium or titanium alloy implants.

The present invention provides compositions and methods for modulating the growth, development, and repair of bone, cartilage, or other connective tissue. Also provided are devices and stimulation waveforms to differentially modulate the behavior of osteoblasts, chondrocytes, and other connective tissue cells to promote proliferation, differentiation, matrix formation, or mineralization for in vitro or in vivo applications. Continuous mode and pulsed segment mode of cell stimulation with charge-balanced signals can be used. Bone, cartilage, and other connective tissue growth is stimulated in part by the release of nitric oxide via electrical stimulation, and can be regulated by simultaneous supply of NO donors and NO synthase inhibitors. Bone, cartilage and other connective tissue growth is stimulated in part by the release of BMP-2 and BMP-7 in response to electrical stimulation that promotes cell differentiation. The methods and devices are useful for promoting bone fracture repair, cartilage and connective tissue repair, and for engineering tissue for transplantation.

The invention belongs to the technical field of plant cell engineering and discloses a method for performing tissue culture and rapid propagation by using Euonymus japonicus L.f. aureo-marginatus Rehd stem tips and segments as explants. The method comprises the steps of conducting cluster bud induction and rooting culture on the stem tips and the stem segments with lateral buds to obtain complete regenerated plants, and sequentially conducting callus induction, callus proliferation and differentiation and rooting culture on the stem segments without lateral buds so as to obtain regenerated plants. The regenerated plants can be obtained in the two ways, operation is simple, the pollution rate and browning rate are low in the culturing process, a large amount of seedlings can be obtained within a short time without seasonal and environmental limits, and the shortcoming of cutting propagation is overcome, meanwhile conditions are provided for genetic transformation and somaclone variation breeding of the seedlings, and the method has significant practical value on theoretical research and landscape application.

In a method and apparatus for analyzing multivariable data sets, a general computerized platform is provided for evaluating the relationship between large number of measurements of sets of variables characterizing components of complex states of a system under induced stimulation or controlled conditions. The linked responses of variables and their temporal relations tell about the network of interactions and their hierarchy. Processing of data sets by a simple neural network gives a matrix of weight parameters, that allow to identify fingerprints of complex states characterized by patterns of measured variable and estimate the interactions between the components characterized by the measured variables. The results are provided numerically and by color-coded presentation indicating dominating relations between variables and strongly responding variables. When applied to dynamic responses of a system, the analysis can construct a schematic hierarchical architecture of the network of interaction between the components of the studied system. Applications in biology include analysis of measurements characterizing responses of molecular components in cells under changes induced by stimuli (e.g. drugs, growth factors, hormones, mutations or forced expression of a proteins), and identification of complex cellular states (e.g. proliferation, differentiation, transformation, starvation, necrosis, apoptosis, and the time dependencies of the above effects).

The invention provides a method for preparing person stem cells with the improved neural restoration function. The method includes the steps that a fusion gene segment containing a plurality of neurotrophic factors is built and recombined to virus expression vectors, and the person stem cells are transfected. Fusion protein containing a plurality of neurotrophic factors can be highly expressed, and the combined effect of the neurotrophic factors can be developed to promote restoration of the neurological functions; meanwhile, the proliferation and differentiation capacity of the stem cells is used for participating in restoration of the neurological functions, and tumorigenicity is avoided; it is expected that the person stem cells can achieve the better treatment effect on nervous system diseases.

The invention provides a preparation method of cross-linking microspheres based on microfluidics. The preparation method comprises the following steps of pushing an injector of a water phase solutionand an oil phase solution which are prepared in advance through a pushing and injecting pump device, enabling a water-oil two-phase solution to form water-in-oil type emulsion liquid drops at an exitchannel of a micro-fluidic chip, and performing illumination at an ultra-violet curing lamp to prepare PEGDA photo-crosslinking microspheres; and enabling the PEGDA photo-crosslinking microspheres tobe charged into a sodium hydroxide solution so as to obtain PEGDA-chitosan cross-linking microspheres, wherein the diameter range of the cross-linking microspheres is 240-440[mu]m, and is controlled through the flow velocity and the flow velocity ratio of the water phase solution and the oil phase solution. The preparation method is flexible and easy to operate. A preparation method of an injecting type chondrocyte composite body comprises the following steps of A1, preparing the cross-linking microspheres; and A2, enabling the cross-linking microspheres to carry chondrocyte. Biological cellsare carried on the cross-linking microspheres having a dual-network hydrogel system, and through proliferation and disintegration of the biological cells, the injecting type cell composite body can beprepared, and is a novel tissue repair material which is widely applied.

The invention relates to the technical field of cryopreservation solutions, in particular to a cryopreservation solution for long-term preservation of human umbilical cord mesenchymal stem cells. The cryopreservation solution for long-term preservation of the human umbilical cord mesenchymal stem cells at least comprises the following preparation raw materials: dimethyl sulfoxide, human serum albumin, a compound electrolyte injection, a sodium chloride injection, a trehalose solution, dextran and a vitamin C injection. The cryopreservation liquid has small toxic and side effects on the human umbilical cord mesenchymal stem cells, can be directly used clinically, obviously improves the safety and biocompatibility of human umbilical cord mesenchymal stem cell retransfusion to human bodies, can improve the motility, proliferation and differentiation capabilities of the human umbilical cord mesenchymal stem cells on the premise of reducing the use amount of DMSO (dimethylsulfoxide), and can be directly retransfused after resuscitation, centrifugal washing is not needed, and damage to cells is reduced.

The invention provides a targeting nucleic acid drug as well as a preparation method and an application thereof. The targeting nucleic acid drug comprises an amino lipid-hyaluronic acid conjugate, a cationic liposome and a complex containing protamine, chondroitin sulfate and a nucleic acid drug, the amino lipid part of the amino lipid-hyaluronic acid conjugate is combined with the cationic liposome, and the cationic liposome wraps the complex. The targeting nucleic acid drug inhibits self-renewal and migration invasion of cancer cells by specifically blocking a Hedgehog signal transduction pathway of cell self-proliferation and differentiation, induces cell apoptosis, and can effectively inhibit recurrence of tumors.

The invention relates to the technical field of cryopreservation solutions, in particular to a human umbilical cord mesenchymal stem cell injection cryopreservation solution and a preparation method thereof. Preparation raw materials of the human umbilical cord mesenchymal stem cell injection cryopreservation liquid at least comprise the following components: human serum albumin, dimethyl sulfoxide, a compound electrolyte injection, a sodium chloride injection and a glucose injection. The human umbilical cord mesenchymal stem cell injection cryopreservation liquid provided by the invention is convenient in material taking, does not violate ethical problems, has small toxic and side effects on cells, is suitable for clinical use, obviously improves the safety and biocompatibility of cell reinfusion to a human body, and can improve the motility and proliferation and differentiation capabilities of umbilical cord mesenchymal stem cells on the premise of reducing the use amount of DMSO (Dimethylsulfoxide); after resuscitation, direct reinfusion is carried out, centrifugal washing is not needed, and damage to cells is reduced.

The invention relates to an application of a 5-substituted-2,4-thiazolidinedione compound as shown in general formula (I) disclosed in the specification or pharmaceutically acceptable salts thereof in the preparation of IGF1R (insulin-like growth factor 1 receptor) function regulating medicaments. The 5-substituted-2,4-thiazolidinedione compound has a better effect in the aspect of inhibiting IGF1R kinase activity, and the IGF1R plays an important role in promoting the proliferation and differentiation of tumor cells, so that the compound provided by the invention can be used for preparing the medicaments for treating tumor diseases.

The invention provides a controlled-release polylactic acid-glycolic acid copolymer microsphere containing a dexamethasone transforming growth factor. A preparation method comprises the following steps: preparing a DEX / PLGA composite microsphere by a water-oil-water emulsion process; positively charging the polylactic acid-glycolic acid copolymer microsphere embedded with DEX through polyethyleneimine of which the surface is covered with positive charges, and mixing TGF-beta3 with a heparin / poly L-lysine solution to form a mixture of which the molar charge ratio is 0-3.15; and mixing TGF-beta3-embedded heparin / poly L-lysine nanoparticles with the PLGA microsphere embedded with the DEX, and covering to obtain the microsphere. The growth-promoting proliferation differentiation action of the growth factor and the anti-inflammatory action of the DEX are compounded; the inflammatory effects caused by a cell attachment carrier are reduced; the controlled-release polylactic acid-glycolic acid copolymer microsphere relatively adapts to cell attachment; the controlled-release polylactic acid-glycolic acid copolymer microsphere is nontoxic and harmless to a living body; a cell scaffold can be degraded by the living body after being treated, and can be used as a carrier for preparing a biological scaffold.

The invention provides a method for promoting directional differentiation and proliferation of mesenchymal stem cells towards neural precursor cells. The method comprises the following steps: firstly, embedding the mesenchymal stem cells in calcium alginate microbeads which are prepared with the presence of a calcium chloride solution and contains gelatin microspheres and chondroitin sulfate; then, adding a neural induction differentiation medium, and cultivating in a rotary reactor, so as to promote the in vitro directional differentiation and proliferation of the mesenchymal stem cells towards the neural precursor cells; and after differentiation, dissolving the calcium alginate microbeads by virtue of a sodium citrate solution, so that differentiated cells are obtained. The method disclosed by the invention is simple to operate and low in cost; by virtue of the gelatin microspheres, a necessary growth space is provided for internal cells of a stent; by virtue of the chondroitin, the biochemical functions of a neural tissue extracellular matrix can be simulated; by virtue of the calcium alginate microbeads, three-dimensional support, which is similar to the extracellular matrix, is provided; and by virtue of the rotary reactor, a necessary micro-gravity environment is provided, so that mass transfer is further enhanced and proliferation of the cells is achieved.