Cross-linking microspheres, and preparation method and application of injecting type chondrocyte composite body of cross-linking microspheres

Abstract

The invention provides a preparation method of cross-linking microspheres based on microfluidics. The preparation method comprises the following steps of pushing an injector of a water phase solutionand an oil phase solution which are prepared in advance through a pushing and injecting pump device, enabling a water-oil two-phase solution to form water-in-oil type emulsion liquid drops at an exitchannel of a micro-fluidic chip, and performing illumination at an ultra-violet curing lamp to prepare PEGDA photo-crosslinking microspheres; and enabling the PEGDA photo-crosslinking microspheres tobe charged into a sodium hydroxide solution so as to obtain PEGDA-chitosan cross-linking microspheres, wherein the diameter range of the cross-linking microspheres is 240-440[mu]m, and is controlled through the flow velocity and the flow velocity ratio of the water phase solution and the oil phase solution. The preparation method is flexible and easy to operate. A preparation method of an injecting type chondrocyte composite body comprises the following steps of A1, preparing the cross-linking microspheres; and A2, enabling the cross-linking microspheres to carry chondrocyte. Biological cellsare carried on the cross-linking microspheres having a dual-network hydrogel system, and through proliferation and disintegration of the biological cells, the injecting type cell composite body can beprepared, and is a novel tissue repair material which is widely applied.

Description

technical field

[0001] The invention relates to the field of tissue engineering, in particular to a preparation method and application of a cross-linked microsphere and an injectable chondrocyte complex. Background technique

[0002] The articular cartilage of the human body ensures the stability of the joints and the movement ability of the human body. Diseases such as trauma or arthritis destroy the normal tissue structure of cartilage, resulting in cartilage defects of various sizes. The cartilage covering the joint surface is a special tissue that ensures the flexibility and accuracy of movement. Its self-repair ability is poor. Once the cartilage is damaged or diseased, the condition will gradually aggravate and directly cause joint pain and limited movement function. Articular cartilage damage caused by various trauma and pathological factors is extremely common in orthopedic clinics. Due to the poor self-repair ability of articular cartilage, it can accelerate its de...

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