Human umbilical cord mesenchymal stem cell injection cryopreservation liquid and preparation method thereof

A technique for stromal stem cells and injection, which is applied to the field of cryopreservation of human umbilical cord mesenchymal stem cell injection and its preparation, can solve the problems of unclear cell growth factors, cytotoxicity, and limitations of freezing conditions, and achieves improved safety. and biocompatibility, less toxic and side effects, and the effect of reducing damage

Active Publication Date: 2021-06-04
朱灏
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  • Abstract
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Problems solved by technology

[0003]The seed bank of human umbilical cord mesenchymal stem cells has been widely used clinically, but there is also the problem that it cannot be applied on a large scale due to the limitation of cryopreservation conditions. No exogenous contamination during storage is the key to the safety and reliability of human umbilical cord mesenchymal stem cells in clinical application. On the other hand, the clinical application of stem cells cultured with animal serum may cause cross-infection of pathogens. Therefore, it is necessary to develop a serum-free cryopreservation solution for human umbilical cord mesenchymal stem cells with clear components.
[0004]The long-term activity of human umbilical cord mesenchymal stem cell seed bank cells is closely related to the cryopreservation environment. Important impact, the existing cryopreservation solution has a greater toxic effect on cells

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  • Human umbilical cord mesenchymal stem cell injection cryopreservation liquid and preparation method thereof

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preparation example Construction

[0058] The second aspect of the present invention provides the preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution, comprising the following steps: adding pre-cooled glucose injection and sodium chloride injection to dimethyl sulfoxide 1. Compound electrolyte injection, mixed uniformly; then add pre-cooled human serum albumin, mixed uniformly to obtain.

[0059] In a preferred embodiment, after the cryopreservation solution is prepared, a pre-cooling step is also included.

[0060] In a preferred embodiment, the pre-cooling is at 2-8°C for at least 30 minutes.

[0061] In a most preferred embodiment, the preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: adding pre-cooled glucose injection, sodium chloride to dimethyl sulfoxide Injection, compound electrolyte injection, mix evenly; then add pre-cooled human serum albumin, mix evenly, and ge...

Embodiment 1

[0067] Embodiment 1 provides a human umbilical cord mesenchymal stem cell injection cryopreservation solution, the preparation raw materials of the cryopreservation solution, by volume percentage, at least include the following components: dimethyl sulfoxide 5%, compound electrolyte injection Liquid 56%, Dextran-40 Glucose Injection 8.5%, 0.9% Sodium Chloride Injection 3%, 10% Glucose Injection 2.5%, 20% Human Albumin 25%.

[0068] The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: absorb DMSO and add it to a 500mL receiver, and slowly add precooled 10% glucose injection and 0.9% sodium chloride injection to it , Dextran-40 Glucose Injection, and Compound Electrolyte Injection, shake while adding until evenly mixed; then slowly add precooled 20% human serum albumin, mix upside down, and precool to obtain; the precooled Pre-cool for 50 min in a medical freezer at 4 °C.

Embodiment 2

[0070] Embodiment 2 provides a human umbilical cord mesenchymal stem cell injection cryopreservation solution, the preparation raw materials of the cryopreservation solution, by volume percentage, at least include the following components: dimethyl sulfoxide 5%, compound electrolyte injection Liquid 40%, Dextran-40 Glucose Injection 5%, 0.9% Sodium Chloride Injection 1%, 10% Glucose Injection 1%, 20% Human Albumin 48%.

[0071] The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: absorb DMSO and add it to a 500mL receiver, and slowly add precooled 10% glucose injection and 0.9% sodium chloride injection to it , Dextran-40 Glucose Injection, and Compound Electrolyte Injection, shake while adding until evenly mixed; then slowly add precooled 20% human serum albumin, mix upside down, and precool to obtain; the precooled Pre-cool for 50 min in a medical freezer at 4 °C.

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Abstract

The invention relates to the technical field of cryopreservation solutions, in particular to a human umbilical cord mesenchymal stem cell injection cryopreservation solution and a preparation method thereof. Preparation raw materials of the human umbilical cord mesenchymal stem cell injection cryopreservation liquid at least comprise the following components: human serum albumin, dimethyl sulfoxide, a compound electrolyte injection, a sodium chloride injection and a glucose injection. The human umbilical cord mesenchymal stem cell injection cryopreservation liquid provided by the invention is convenient in material taking, does not violate ethical problems, has small toxic and side effects on cells, is suitable for clinical use, obviously improves the safety and biocompatibility of cell reinfusion to a human body, and can improve the motility and proliferation and differentiation capabilities of umbilical cord mesenchymal stem cells on the premise of reducing the use amount of DMSO (Dimethylsulfoxide); after resuscitation, direct reinfusion is carried out, centrifugal washing is not needed, and damage to cells is reduced.

Description

technical field [0001] The invention relates to the technical field of cryopreservation solution, in particular to a cryopreservation solution of human umbilical cord mesenchymal stem cell injection and a preparation method thereof. Background technique [0002] Human umbilical cord mesenchymal stem cells refer to a kind of pluripotent stem cells isolated from umbilical cord. Compared with other sources of mesenchymal stem cells, they have obvious advantages in clinical application, such as easy to obtain materials, easy to collect, cryopreservation and relatively pure, etc. High differentiation potential, can be differentiated in multiple directions, such as osteoblasts, chondrocytes, hepatocytes, insulin-like cells, nerve cells and cardiomyocytes, etc., and there are no ethical issues. At present, human umbilical cord mesenchymal stem cells have been applied in the clinical field and achieved encouraging therapeutic effects. [0003] The seed bank of human umbilical cord ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 朱灏
Owner 朱灏
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