Human umbilical cord mesenchymal stem cell injection cryopreservation liquid and preparation method thereof
A technique for stromal stem cells and injection, which is applied to the field of cryopreservation of human umbilical cord mesenchymal stem cell injection and its preparation, can solve the problems of unclear cell growth factors, cytotoxicity, and limitations of freezing conditions, and achieves improved safety. and biocompatibility, less toxic and side effects, and the effect of reducing damage
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[0058] The second aspect of the present invention provides the preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution, comprising the following steps: adding pre-cooled glucose injection and sodium chloride injection to dimethyl sulfoxide 1. Compound electrolyte injection, mixed uniformly; then add pre-cooled human serum albumin, mixed uniformly to obtain.
[0059] In a preferred embodiment, after the cryopreservation solution is prepared, a pre-cooling step is also included.
[0060] In a preferred embodiment, the pre-cooling is at 2-8°C for at least 30 minutes.
[0061] In a most preferred embodiment, the preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: adding pre-cooled glucose injection, sodium chloride to dimethyl sulfoxide Injection, compound electrolyte injection, mix evenly; then add pre-cooled human serum albumin, mix evenly, and ge...
Embodiment 1
[0067] Embodiment 1 provides a human umbilical cord mesenchymal stem cell injection cryopreservation solution, the preparation raw materials of the cryopreservation solution, by volume percentage, at least include the following components: dimethyl sulfoxide 5%, compound electrolyte injection Liquid 56%, Dextran-40 Glucose Injection 8.5%, 0.9% Sodium Chloride Injection 3%, 10% Glucose Injection 2.5%, 20% Human Albumin 25%.
[0068] The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: absorb DMSO and add it to a 500mL receiver, and slowly add precooled 10% glucose injection and 0.9% sodium chloride injection to it , Dextran-40 Glucose Injection, and Compound Electrolyte Injection, shake while adding until evenly mixed; then slowly add precooled 20% human serum albumin, mix upside down, and precool to obtain; the precooled Pre-cool for 50 min in a medical freezer at 4 °C.
Embodiment 2
[0070] Embodiment 2 provides a human umbilical cord mesenchymal stem cell injection cryopreservation solution, the preparation raw materials of the cryopreservation solution, by volume percentage, at least include the following components: dimethyl sulfoxide 5%, compound electrolyte injection Liquid 40%, Dextran-40 Glucose Injection 5%, 0.9% Sodium Chloride Injection 1%, 10% Glucose Injection 1%, 20% Human Albumin 48%.
[0071] The preparation method of the human umbilical cord mesenchymal stem cell injection cryopreservation solution comprises the following steps: absorb DMSO and add it to a 500mL receiver, and slowly add precooled 10% glucose injection and 0.9% sodium chloride injection to it , Dextran-40 Glucose Injection, and Compound Electrolyte Injection, shake while adding until evenly mixed; then slowly add precooled 20% human serum albumin, mix upside down, and precool to obtain; the precooled Pre-cool for 50 min in a medical freezer at 4 °C.
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Abstract
Description
Claims
Application Information
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