21results about How to "Prevent recurrence" patented technology

The invention discloses a double-layer microneedle patch and a preparation method thereof.The preparation method of the double-layer microneedle patch comprises the following steps that firstly, 3D printing is conducted through polydimethylsiloxane, and a PDMS mold similar to a machining microneedle is obtained; dispersing the medicine into the sericin solution to serve as an outer layer solution; dispersing germanium nanoparticles into the polycaprolactone solution to obtain an inner layer solution; and finally, adding the outer-layer solution into the needle-shaped groove of the PDMS mold, so that the needle-shaped groove is filled with the outer-layer solution, drying, adding the inner-layer solution, drying, and demolding to obtain the double-layer microneedle patch. When the double-layer microneedle patch is used for drug delivery, drugs can be dissolved and released in a short time, the inner layer is polycaprolactone (PCL) carrying photo-thermal agent germanium nanoparticles, degradation is slow, a photo-thermal effect is generated under irradiation of 808nm near-infrared light, residual tumor cells are induced to die immunogenicity, an immune microenvironment is programmed in a time sequence, and the double-layer microneedle patch has the advantages of being high in sensitivity, high in sensitivity and good in stability. Postoperative recurrence and metastasis of tumors are efficiently inhibited, and meanwhile operative wound repair is promoted.

The invention discloses an antigen peptide polymer vesicle tumor vaccine as well as a preparation method and application thereof. The antigen peptide polymer vesicle tumor vaccine is obtained by assembling and cross-linking a polymer pre-grafted with antigen peptide and a block polymer and loading an immunologic adjuvant through electrostatic interaction. The vesicle system disclosed by the invention has the advantages of small size, simplicity and controllability in preparation, excellent biocompatibility, high in-vivo circulation stability, targeting of main immune organs, intracellular delivery of antigen peptides and the like. The vesicle nano vaccine displaying the antigen peptide on the surface and encapsulating the immunologic adjuvant can promote dendritic cell maturation and antigen presentation, improve the specific response of mice to tumor-associated antigens, effectively inhibit tumor growth, remarkably prolong the lifetime of tumor-bearing mice and induce a strong immune protection effect, and is high in safety and easy to popularize. A multifunctional'ready-to-use 'nano vaccine concept is embodied, a flexible platform is provided, and different peptide antigens and / or adjuvants can be used for treating different cancers.

The present invention discloses an immune-enhancing targeted hollow manganese dioxide radiotherapy sensitizer and its preparation method and application, which belongs to the field of tumor treatment technology. Aiming at the problems of short circulation time and poor targeting of radiotherapy sensitizers, the present invention adopts an engineered extracellular vesicle modification method to improve the circulation time, so that it has tumor targeting; Aiming at the problem of poor improvement effect of hypoxic microenvironment in the prior art, the present invention prepares hollow structure MnO2 by template self-etching method to improve the catalytic efficiency of overexpressed hydrogen peroxide in tumor cells, and uses metformin, a cell respiration inhibitory drug, in synergy to reduce cell oxygen consumption, improve hypoxic microenvironment from two aspects, and enhance radiotherapy sensitization effect; At the same time, the present invention effectively regulates the immunosuppressive microenvironment of tumors by reducing manganese ions generated by sensitizers, and activates the cGAS-STING pathway to enhance the activity of natural immune cells, release immune factors, and inhibit the metastasis and recurrence of tumors after radiotherapy.

The invention relates to the technical field of biology, in particular to a targeting peptide modified nano-micelle co-loaded with near-infrared fluorescent dye and an immunologic adjuvant, a preparation method and an application of the targeting peptide modified nano-micelle co-loaded with the near-infrared fluorescent dye and the immunologic adjuvant. A nano-carrier of the nano-micelle is a PLGA-PEG nano-carrier, a targeting peptide is a TMTP1 targeting peptide, a near-infrared fluorescent dye is indole green, and an immunologic adjuvant is MPLA. The nano-micelle can target ovarian cancer primary lesion and metastatic lesion, increase aggregation of micelles in tumor tissues, generate PTT effect through laser irradiation under the guidance of near-infrared fluorescence, cause immune death of tumor cells, release tumor-associated antigens, and promote DC cell recognition of presentation antigens and DC cell activation under the assistance of an immunologic adjuvant, so that the tumor-associated antigens can be degraded, and the tumor-associated antigens can be degraded. In addition, macrophages and NK cells can be collected, T cell immune response is induced, secretion of immunomodulatory factors is promoted, other tumor cells not irradiated by laser are recognized and killed, immune memory is generated, and tumor relapse is inhibited.

The present invention discloses a lymph node targeted tumor in situ vaccine and a preparation method and application thereof, which belongs to the field of biomedicine technology. The immune adjuvant CpG is coated on mannose nanoparticles to obtain a CpG nanoadjuvant, and then an MMP responsive gel is used as a delivery system to deliver the CpG nanoadjuvant and trametinib together, thereby achieving sustained in situ antigen production, lymph node drainage and APCs presentation, and further activating anti-tumor immune response. The vaccine has a use in preparing vaccine drugs for treating tumors, and as an effective cancer vaccine for immunotherapy, achieves targeting to lymph nodes to induce anti-tumor immunity.

The invention discloses application of niclosamide in preparation of a medicine for treating endometriosis, and belongs to the technical field of biological medicine. In-vivo and in-vitro experiments prove that the niclosamide can down-regulate the expression of primary endometriosis mesenchymal cells CD47 through a JAK / STAT3 pathway, enhance the phagocytosis of macrophages and play a role in effectively treating endometriosis; the invention provides the preparation method of the niclosamide microneedle, the preparation method is simple, the prepared LPHM-coated DDI NPs shows synergistic cytotoxicity and apoptosis induction activity in endometriosis mesenchymal cells, growth and recurrence of a focus of a subcutaneous endometriosis mouse model can be effectively inhibited through tumor ablation and anti-angiogenesis, and the niclosamide microneedle has a good application prospect. Rapid and effective treatment of endometriosis is realized in a microneedle patch mode.

This invention discloses the application of a PDLIM2 gene overexpression viral vector in the preparation of drugs for treating podocyte disease, wherein the nucleotide sequence of the PDLIM2 gene is shown in SEQ ID NO:1. This invention is the first to discover and verify the core role of the PDLIM2 gene in podocyte protection, confirming its significant downregulation in DKD and FSGS disease models, and demonstrating through functional experiments that PDLIM2 overexpression can effectively stabilize the podocyte cytoskeleton, inhibit apoptosis, and reduce proteinuria, thus establishing it as a key target gene for the treatment of podocyte disease. This invention is the first to intervene at the gene level in the core link of podocyte damage—cytoskeleton stability. By specifically upregulating the expression of PDLIM2 in podocytes, it directly enhances their intrinsic cytoskeleton support and anti-damage ability, achieving a fundamental shift in treatment strategy from "symptomatic support" to "causal repair."

An anti-CD47 monoclonal antibody and the use thereof in combination with a monoclonal antibody, a bispecific antibody and / or a tumor therapeutic agent, wherein the anti-CD47 monoclonal antibody is secreted by a hybridoma cell line with a deposit number of CCTCC NO: C2018135.

The present application belongs to the technical field of fruit tree disease prevention and control, and particularly relates to a method for removing sporadic citrus Huanglongbing trees in citrus orchards. According to the epidemiological characteristics of citrus Huanglongbing, the control strategy of 'completely removing diseased plants, strictly preventing and controlling citrus psyllids, and comprehensively preventing and controlling' is implemented at sporadic disease points in the diffusion front area. The sporadic disease point orchards are promptly cleaned of diseased trees, and the diffusion front area is comprehensively prevented and controlled to prevent the disease from occurring again. In this way, disease information and risk warnings can be promptly released, the removal of diseased trees and the strict prevention and control of citrus psyllids can be achieved, the invasion and reproduction of exogenous psyllids can be hindered, the risk of healthy seedlings getting sick can be reduced, and the spread of citrus Huanglongbing can be blocked. The present application provides technical support for delaying the further spread of citrus Huanglongbing and saves human and material resources.

The application discloses a blended yarn conveying and winding equipment with a deburring function, a fixed base is arranged in a hollow frame structure, a winding drum is fixedly connected to one side of the top of the fixed base, a support frame is arranged in a back-shaped frame structure, the bottom of the support frame is fixedly connected to the top of the fixed base, a deburring device is fixedly connected to the side of the support frame, a tensioning device is rotatably connected to the inner wall side of the support frame below the deburring device, the deburring device comprises a straightening drum, a deburring rod is fixedly connected to the side of the straightening drum, a driving shaft of a first motor is fixedly connected to the side of the straightening drum, the fixed end of the first motor is fixedly connected to the side of the support frame, and the side of the straightening drum is rotatably connected to the side of the support frame.

The invention relates to the technical field of biomedical materials, in particular to radio frequency responsive polyion liquid as well as a preparation method and application thereof. The radio frequency responsive polyion liquid comprises a temperature-sensitive block formed by polymerization of N-isopropylacrylamide and a functional block formed by polymerization of a monomer as shown in a formula I; formula I: [cation] X, the cation being selected from the group consisting of 1-vinyl-3-ethylimidazolium, 1-vinyl-3-butylimidazolium, 1-vinyl-3-hexylimidazolium, benzyl ethyl trimethyl ammonium, or methacryloyloxyethyl trimethyl ammonium; x is a halide ion; and in the block copolymer, anions combined with cations in the functional block are combretastatin phosphate ions. Through the innovative design of the block copolymer, temperature sensitivity, radio frequency responsiveness and treatment activity are integrated, and a multifunctional integrated intelligent material platform is successfully constructed.

This invention provides a nucleic acid aptamer that targets and binds to the IGF2BP2 protein and its application, belonging to the field of pharmaceutical technology. This invention is the first to design a highly specific nucleic acid aptamer targeting the RNA recognition protein IGF2BP2, obtaining a nucleic acid aptamer that can target and specifically bind to the IGF2BP2 protein (see SEQ ID No. 1-2). This nucleic acid aptamer can effectively inhibit the activity of tumor cells, tumor stem cells, and breast cancer brain metastases, breast cancer lung metastases, breast cancer liver metastases, breast cancer kidney metastases, and breast cancer bone metastases, providing a new drug component for the treatment of malignant and refractory tumors (especially glioblastoma and distant metastatic breast cancer). Furthermore, it was discovered that modification with N6-methyldeoxyadenosine monophosphate can further enhance the specificity of the nucleic acid aptamer and strengthen its efficacy in inhibiting tumor cell activity.

This invention discloses a collaborative management system and method for river cyanobacteria based on AI-based early warning and targeted drug delivery. The system comprises an intelligent sensing and early warning layer, a central decision-making and control layer, and a precise targeted execution layer. The intelligent sensing and early warning layer collects environmental data of the target river through an air-ground-water three-dimensional monitoring network and uses an AI prediction model to provide early warning of cyanobacteria outbreak risks. The central decision-making and control layer receives the risk warnings and, in conjunction with algae spatial distribution, hydrological conditions, and weather forecasts, generates management strategies and control instructions based on a preset rule base and optimization algorithms. The precise targeted execution layer includes at least one unmanned targeted drug delivery vessel that receives the control instructions and executes drug delivery to complete the river cyanobacteria control. This invention achieves a shift from passive response to active intervention and from extensive management to precise control, offering advantages such as high efficiency, environmental friendliness, and long-term effectiveness.

The present application belongs to the technical field of steel pipe detection, and particularly relates to a seamless steel pipe toughness performance defect optimization method and system. The method compares the test output value with the preset comparison interval, identifies the test parameters that cause the test output value to deviate, and classifies the test parameters as positive parameters or negative parameters according to the deviation direction. The test parameters that cause the test output value to deviate are classified, the test parameters are determined as impact parameters or necessary parameters, the impact parameters are defined as optimization items, and the necessary parameters are defined as risk items. Key defects are identified, and the severity is quantitatively evaluated to provide a risk warning signal for production managers. The present application provides a data basis for exploring the causes of defects through identification and classification of defect causes, avoids indiscriminate treatment of all test parameters that cause deviation, improves the efficiency of problem diagnosis and solution, and realizes risk warning and active prevention.

The application relates to the technical field of emergency management, and discloses an emergency response and early warning system for a sudden combustion and explosion event, which comprises a multi-source data access module, which is used for collecting target equipment data and environment benchmark equipment data of energy storage equipment or a centralized charging site in real time, including temperature, current, voltage, fan state and smoke / odor sensor data, and pre-processing the collected target equipment data and environment benchmark equipment data; an abnormal index preset module, which is used for presetting a composite abnormal index representing early signs of thermal runaway and risk grading standards; and a same-site benchmarking module, which is based on the collected target equipment data and environment benchmark equipment data. The same-site benchmarking module is adopted, the environment benchmark data and abnormal acceleration change trend calculation are carried out, the technical effect of identifying the composite abnormal index in an extremely early stage is achieved, early warning is realized, and the problem that the safety window period for the operation party to take initial disposal actions is short is solved.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to an oral and external compound type combined preparation for treating vitiligo and a preparation method of the oral and external compound type combined preparation. The skin antibacterial liquid is prepared from the following raw materials: a traditional Chinese medicine concentrated solution, polyethylene glycol 200, nano titanium dioxide, grape seed oil, polydimethylsiloxane and absolute ethyl alcohol; the dolichos peptide tablet is prepared from the following raw materials: traditional Chinese medicine dry paste powder, bone collagen peptide, soybean peptide powder, calcium carbonate, microcrystalline cellulose, D-mannitol, zinc gluconate, sodium selenite and purified water. The traditional Chinese medicine composition treats vitiligo in an integral-local synergistic manner, provides nutrients necessary for melanin generation, improves skin microcirculation, enhances body antioxidant and antibacterial capabilities, reduces damage of an immune system to melanin color cells, accelerates pigment recovery of leukoplakia at a focus position, and maintains a healthy state of skin.

The invention relates to a dendrimer-based polypeptide nano vaccine as well as a preparation method and application thereof. The polypeptide nano vaccine is obtained by loading MnO2 nano particles on a functionalized dendrimer and wrapping a polypeptide antigen. After the polypeptide nano vaccine disclosed by the invention is injected into a mouse body through peritumoral injection, MnO2 plays a chemical kinetic treatment role through a Fenton-like reaction, and tumor cells are induced to generate immunogenic cell death, so that dendritic cell maturation is promoted; meanwhile, the polypeptide nano vaccine can activate an immune system and initiate potent immune response by activating a cGAS-STING pathway and being cooperated and combined with presentation of a polypeptide antigen OVA257-264, a new chemical kinetics / immune combined treatment strategy is provided, and the polypeptide nano vaccine has a remarkable inhibition effect on tumor growth and recurrence and has potential application value in the field of biological medicine.

A modified immune cell and a use thereof in immunotherapy, the immune cell being a PD-1+T cell from peripheral blood. The immunotherapy is used in cancer treatment or treatment and prevention of diseases related to viral infections.

The invention discloses application of a PDLIM2 gene overexpression virus vector in preparation of a medicine for treating podocytosis. The nucleotide sequence of a PDLIM2 gene is as shown in SEQ ID NO: 1. The core effect of the PDLIM2 gene in podocytosis protection is found and verified for the first time, it is confirmed that the expression of the PDLIM2 gene in DKD and FSGS disease models is remarkably reduced, and functional experiments prove that the overexpression of the PDLIM2 can effectively stabilize podocytoskeleton, inhibit apoptosis and relieve proteinuria, so that the PDLIM2 gene is determined as a key target gene for treating podocytosis. According to the invention, the stability of the cytoskeleton, which is a core link of podocyte injury, is intervened from the gene level for the first time. The expression of the PDLIM2 in the podocyte is specifically up-regulated, so that the internal cytoskeleton support and damage resistance of the podocyte are directly enhanced, and the fundamental treatment strategy conversion from symptomatic support to cause repair is realized.

A keloid mechanical balance microneedle, a preparation method and an application in preparing a product for preventing keloid recurrence, wherein the preparation method of the keloid mechanical balance microneedle is as follows: firstly, according to the average thickness of postoperative scar tissue of an object, a preliminary skin-scar finite element model composed of a scar structure and a skin structure is constructed, and the thickness of each position of the whole scar structure is the same; then, the keloid mechanical balance microneedle is finally obtained through four steps. The keloid mechanical balance microneedle is prepared according to the postoperative scar tissue of an object, and through the keloid mechanical balance microneedle, the stress distribution between the keloid and the surrounding skin can be uniform, and the keloid recurrence can be effectively prevented.

The invention discloses a pyroelectric nano-particle based on a black phosphoryl-Fe < 3 + > complex as well as a preparation method and application of the pyroelectric nano-particle. The pyroelectric nanoparticle comprises a cell membrane and a black phosphorus-Fe < 3 + > complex wrapped in the cell membrane. The preparation method comprises the following steps: uniformly shaking the cell membrane and the black phosphoryl-Fe < 3 + > complex in an air bath, and extruding by using a liposome extruder. Under laser on / off irradiation, the prepared nano-particles are triggered by temperature change to generate pyroelectric catalysis for water decomposition in tumor intercellular fluid, aggregation of the nano-drug at a tumor site is improved, tumor cells are further efficiently killed, and in the catalysis process, along with generation of oxygen and ROS, polarization of tumor-related macrophages is further induced, so that the tumor-related macrophages are effectively killed, and the tumor-related macrophages are effectively killed. Anti-tumor immune response is activated, and tumor recurrence and metastasis are inhibited.