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Targeted gene carrier material based on graphene oxide and its preparation and application
InactiveCN102274521ALarge specific surface areaEasy to retouchGenetic material ingredientsInorganic non-active ingredientsTumor targetFolic acid
The invention relates to a tumor-targeted gene vector material and a preparation method and use thereof. In the invention, functional graphene oxide is used as a targeted transport vector of genomic molecules such as siRNA. The preparation method comprises: connecting folic acid tumor cell targeted molecules and amino-terminated polyethylene glycol by amido bonds; connecting the graphene oxide with the coupling material by amido bonds to obtain the tumor targeted functional graphene oxide; anchoring aminated molecules with an aromatic conjugated structure onto the surface of the graphene oxide through pi-pi conjugation; and finally, preparing the tumor targeted high-efficiency gene transport system by static attraction between the positively charged functional graphene oxide and the negatively charged genomic molecules. The vector material has a high targeting performance for tumor cells in which folic acid receptor is expressed more highly, and after being loaded with siRNA, the corresponding target gene level and related protein expression can be reduced. The invention provides a new method for the in-vivo targeted transportation of genes.
Owner:TIANJIN MEDICAL UNIV
Environment protection type efficient aerating powder for shield construction
ActiveCN101462844AHigh foaming ratioEasy to operateTransportation and packagingMixingFoaming agentSlag
The invention discloses an environmental protection high efficiency foaming agent for shield construction. The foaming agent comprises the following components in percentage by weight: 5 to 20 percent of aliphatic alcohol polyethenoxy ether sulfate, 1 to 8 percent of alcohol amide, 0.5 to 8 percent of alcohol ether, and 64 to 93.5 percent of water. The foaming agent for shield construction has a high foaming ratio and produces abundant and fine foams with even diameter distribution. The produced bubbles have small pores, good lubricity and stability, simple production operation, and lower cost. The foaming agent can better meet the requirements of the slag soil improvement during the process of earth pressure balanced shield.
Owner:GUANGZHOU AORUN SYNTHETIC MATERIAL
Kit for extracting nucleic acid from sputum and extraction method
The invention relates to the field of biology, and relates to a method of extracting a nucleic acid by taking sputum as a sample and application of the extraction method. The invention provides a dedicated kit for extracting a nucleic acid from sputum and an extraction method. The specific extraction method comprises the following steps: adding a digestive juice KB which is 1-5 times of the sputum into the sputum, vibrating and uniformly mixing the liquid, adding protease to the liquid, keeping warm at the temperature of 37-65 DEG C for 1-5 hours, adding a binding solution BS which is 0.5-3 times of the sputum sample capacity, after vibrating and uniformly mixing, enabling the complete solution to pass through a nucleic acid adsorption column, allowing the nucleic acid in the sample to be bound with a silica membrane of the adsorption column, conducting steps of washing, eluting and the like, and extracting the nucleic acid from the sputum. The nucleic acid extracted by the kit provided by the invention is high in yield and purity, and can be directly used for molecular biological detection.
Owner:宁波有成生物医药科技有限公司
RNA type sample preservation and dilution liquid and preparation thereof
ActiveCN105506129AProtection from degradationInhibit RNase activityMicrobiological testing/measurementPolymerase LHigh pressure
The invention aims at providing RNA type sample preservation and dilution liquid and a preparation method thereof and belongs to the scopes of dilution and preservation of ribonucleic acid (RNA) type samples in the technical field of polymerase chain reactions (PCR). The preparation method comprises the steps of adding a certain amount of RNA preservative and chemical reagent to deionized purified water; adjusting pH value to 4.8-5.2, and then maintaining the constant volume; conducting sealing and autoclaved sterilization, and leaving the mixture for standby application at the room temperature. The RNA type sample preservation and dilution liquid is a white transparent solution, has the advantages of being stable, easy to preserve, convenient and the like and is applicable to preservation and / or dilution of RNA type samples such as serum, plasma, tissues, urine and secreta. After the RNA samples are immersed in the RNA type sample dilution and preservation liquid, RNA in fresh histocyte can be preserved in a good condition for one day at the temperature of 37 DEG C, a week at the temperature of 25 DEG C, one month at the temperature of 4 DEG C and a long time at the temperature of minus 20 DEG C or minus 80 DEG C.
Owner:GUANGZHOU BDS BIOLOGICAL TECH CO LTD
Preparation method of flow cytometry sample for chrysanthemum
InactiveCN104374617AEfficient removalEnsure stabilityPreparing sample for investigationStainingDecomposition
The invention belongs to the field of the detection on flow cytometry, and provides a preparation method of a flow cytometry sample for chrysanthemum. The method comprises the following steps: carrying out a splitting decomposition step, namely, carrying out the splitting decomposition on a chrysanthemum sample, filtering and centrifuging to obtain a cell nucleus to be dyed; and carrying out a dyeing step, namely, dyeing the cell nucleus to be to be dyed to obtain the dyed cell nucleus. According to the flow cytometry analysis, an appropriate cell lysis buffer solution is adopted, so that cytoplasmic fragments remained in the complete cell nucleus of the chrysanthemum can be effectively eliminated, the stability of the cell nucleus in suspension liquid can be maintained, and the agglutination of the cell nucleus in the suspension liquid can be prevented; DNA can be protected from degradation; an appropriate environment is provided for the exclusive nucleus DNA chemical staining, and the adverse impact of the cytoplasm component on the dyeing can be effectively reduced; by optimizing the sample preparation flow, the disturbance peak in the computer testing can be effectively reduced, and the precision, accuracy and stability of a detection result can be improved.
Owner:BEIJING AGRO BIOTECH RES CENT
Application of rice OsBBTI4 protein gene to improving resistance of rice to rice blast
ActiveCN108517333AIncrease resistanceImprove disease resistanceProtease inhibitorsFermentationBiotechnologyTrypsin inhibitor
The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Nucleic-acid composite nano-drug self-assembled and constructed based on coordination of metal ions, and preparation method and application of nucleic-acid composite nano-drug
ActiveCN111317826AIncrease load factorEasy to makePowder deliveryOrganic active ingredientsPharmaceutical drugTherapeutic effect
The invention provides a nucleic-acid composite nano-drug self-assembled and constructed based on coordination of metal ions, and a preparation method and application of the nucleic-acid composite nano-drug. The nucleic-acid composite nano-drug can be applied to drugs for treating skin; the nucleic-acid composite nano-drug is formed through driving a nucleic acid nano-material with relatively goodbiocompatibility to assemble a nucleic acid nano-carrier with uniform size and controllable particle size and loading a drug based on the coordination of the metal ions; or the nucleic-acid compositenano-drug is formed through driving assembling of a conjugate of a chemotherapy drug and nucleic acid; or the nucleic-acid composite nano-drug is formed through driving a gene drug or a gene drug ofan embedded drug. According to the method provided by the invention, the complexity of a design and synthesis process of a nucleic acid nano-material by a nucleic acid nanotechnology is improved, thebiotoxicity of the nano-material is effectively lowered, the hydrophobic problem of the drug can be solved, and the treatment effect of the drug is improved; and in addition, the nano-composite nano-system has relatively good skin tissue permeability and provides a novel way of think for the field of local drug administration.
Owner:SHANGHAI JIAO TONG UNIV
Application of reduction response polymersome nano-drug in preparation of brain tumor treatment drugs
ActiveCN107998082AHigh drug loading efficiencyImprove in vitro stabilityOrganic active ingredientsMacromolecular non-active ingredientsIn vivoGlioma cell
The invention discloses application of a reduction response polymersome nano-drug in preparation of brain tumor treatment drugs. Block copolymers PEG-P(TMC-DTC), PEG-P(LA-DTC), PEG-P(TMC-DTC)-PEI, PEG-P(LA-DTC)-PEI, PEG-P(TMC-DTC)-Sp, PEG-P(LA-DTC)-Sp and targeted polymers thereof are combined in different ratios, and dual-targeted reduction-sensitive reversible crosslinked vesicles of different targeted molecular proportions can be prepared. Through the hydrogen-bond interaction or other electrostatic interaction between great hydrophilic inner cavities of the crosslinked vesicles and / or PEI-spermine and protein drugs or gene drugs, efficient encapsulation of micromolecular chemotherapeutic drugs, the protein drugs and the gene drugs can be achieved. By means of the in-vivo dual-targeteddrug-carrying crosslinked vesicles, more efficient blood brain barrier crossing, higher tissue penetration depth and endocytosis of more glioma cells can be achieved, and the reduction response polymersome nano-drug is very potential in treating brain gliomas and efficient.
Owner:SUZHOU UNIV
Method for extracting and transforming excrement DNA in quantity
ActiveCN109486809AProtection from degradationMeet cold chain transportationMicrobiological testing/measurementDNA preparationStool dnaFeces
The invention discloses a method for extracting and transforming excrement DNA in quantity. The method comprises the steps of collecting 1g-5g of excrement into a tube containing protecting liquid, adding cracking liquid for adequately cracking so as to remove an inhibitor, precipitating nucleic acid by virtue of an organic solvent, resuspending precipitates so as to obtain nucleic acid for nucleic acid extraction or nucleic acid transformation, carrying out a series of combined washing steps, and finally carrying out elution, so as to obtain excrement DNA. The method comprises the steps of extraction and transform of excrement DNA and combination of extraction and transformation. The method has the characteristics of low cost, high utilization rate, good repeatability, simplicity and rapidness; a large number of excrement DNA or transformed excrement DNA can be obtained, and more possibilities can be provided for the transformation or the downstream detection.
Owner:杭州艾维克生物科技有限公司
Fecal nucleic acid preserving fluid and preparation method and application thereof
The invention relates to a fecal nucleic acid preserving fluid as well as a preparation method and application thereof. The fecal nucleic acid preserving solution comprises a Tris-HCl buffer solutionwith the concentration being 0.01 to 1M, ethylenediaminetetraacetic acid (EDTA) with the concentration being 0.01 to 1M, sodium chloride with the concentration being 0.01 to 0.5 M, sodium citrate withthe concentration being 0.01 to 0.5 M and absolute ethyl alcohol with the volume concentration being 10 to 30 percent, wherein the pH value of the preservation solution is 7.0 to 10.0. The fecal nucleic acid preserving fluid provided by the invention can effectively maintain the stability of nucleic acid in feces, and is convenient for a user to sample at any time and transport at normal temperature. And downstream detection of intestinal flora is not influenced.
Owner:SHANGHAI REALBIO TECH CO LTD
Toughening and plasticizer migration-resisting polyvinyl chloride material as well as preparation method and application thereof
The invention provides a toughening and plasticizer migration-resisting polyvinyl chloride material as well as a preparation method and application thereof and relates to the technical field of macromolecular materials. The preparation method of the toughening and plasticizer migration-resisting polyvinyl chloride material comprises the following steps: preparing laminar di-hydroxyl metal oxide through a high-temperature hydrothermal method; carrying out surface modification on the laminar di-hydroxyl metal oxide by utilizing dopamine, dopamine hydrochloride or a dopamine derivative, so as to obtain polydopamine modified laminar di-hydroxyl metal oxide; finally, mixing polyvinyl chloride, a plasticizer, a stabilizer and the polydopamine modified laminar di-hydroxyl metal oxide to obtain modified polyvinyl chloride. The prepared material has the advantages of relatively remarkable toughening and plasticizer migration-resisting properties and the like, and the compatibility of inorganic nanoparticles and the polyvinyl chloride is improved; furthermore, polydopamine can be used for preventing a polymer matrix from being degraded in an UV (Ultraviolet) illumination process, the performance stability of the polyvinyl chloride material is improved and the service life of the polyvinyl chloride material is prolonged.
Owner:XIAMEN UNIV OF TECH
Preparation method of high-purity 2-alkyl-4-isothiazoline-3-ketone
The invention discloses a preparation method of high-purity 2-alkyl-4-isothiazoline-3-ketone. The preparation method comprises the following steps: suspending amide into a reaction medium inert solvent; introducing CMI and MIT mixture tail gas or MIT tail gas generated by the previous step, so as to obtain an amide suspending solution with proper hydrogen chloride content; and introducing a chlorinating reagent. According to the preparation method, a byproduct, namely hydrogen chloride, has an inhibition effect on the generation of 5-chloro-2-alkyl-4-isothiazoline-3-ketone. The content of the hydrogen chloride is increased through utilizing reaction raw materials and solvents; and finally, the high-purity 2-alkyl-4-isothiazoline-3-ketone is obtained.
Owner:甘肃开美高精细化工有限公司
Preparation process of environment-friendly water-based ink
The invention discloses a preparation process of environment-friendly water-based ink. The preparation process comprises the following steps: step 1, weighing raw materials; step 2, adding deionized water into a dispersion cylinder, then adding a pigment, carrying out ultrasonic treatment at normal temperature for 10 minutes to prepare an aqueous dispersion liquid of the pigment, then adding a wetting agent and half of a dispersing agent, and carrying out high-speed dispersion and grinding to obtain color paste; and step 3, mixing the color paste with the polyacrylate emulsion, and adding a modified filler, a dispersing agent and a defoaming agent to obtain the environment-friendly water-based ink. According to the invention, the water-based polyacrylate emulsion is used as a film-formingsubstance of the ink; the emulsion has high film-forming property and adhesive force; by adding the modified filler, the phenomenon of ink aging caused by ultraviolet rays can be effectively prevented, the damage caused by the ultraviolet rays to an organic resin base material is reduced, and the water-based ink has water resistance and ultraviolet-resistant stability at the same time; the raw materials are green and environment-friendly, and the water-based ink which is environment-friendly, excellent in comprehensive performance and capable of effectively defending ultraviolet rays is obtained.
Owner:郑梦平
Cytomembrane nano-vesicles and production method thereof
PendingCN111235108AProtection from degradationEfficient intakeNervous disorderNervous system cellsSingle cell suspensionCell membrane
The invention discloses cytomembrane nano-vesicles and a production method thereof. The cytomembrane nano-vesicles are composed of bi-molecule-layer lipid membranes and contents wrapped by the bi-molecule-layer membranes, wherein the membranes and the contents of the cytomembrane nano-vesicles all come from metrocytes used for production. The production method of the cytomembrane nano-vesicles comprises the following step: sequentially and repeatedly extruding a single-cell suspension to pass through polycarbonate membranes, with different pore diameters, of an extruder to obtain a clear solution, and conducting gradient centrifugation, separation and purification on the clear solution to obtain the cytomembrane nano-vesicles. The production method of the cytomembrane nano-vesicles is highin yield, the nano-vesicles are stable in property, the product uniformity is good, operation is simple and convenient, the quality is controllable, and the repeatability is high, so that large-scaleproduction is facilitated, and the cytomembrane nano-vesicles can be applied to treatment of Parkinson's disease.
Owner:FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
Application of targeted reduction response vesicular nanometer medicines in preparation of brain tumor treatment medicines
ActiveCN107998081ANot degraded to prevent leakagePrevent leakageOrganic active ingredientsMacromolecular non-active ingredientsBiocompatibility TestingApoptosis
The invention discloses application of targeted reduction response vesicular nanometer medicines in preparation of brain tumor treatment medicines. Reduction sensitive reversible crosslinking vesiclesbased on block polymers PEG-P(TMC-DTC), PEG-P(LA-DTC), PEG-P(TMC-DTC)-PEI, PEG-P(LA-DTC)-PEI, PEG-P(TMC-DTC)-Sp or PEG-P(LA-DTC)-Sp and targeting polymers with ANG as the targeting molecules can efficiently wrap small-molecular chemotherapy medicines, protein medicines and gene medicines sensitive to brain glioma cells. The medicine-carrying vesicles can efficiently permeate through a blood brainbarrier in vivo to enter tumor substances and can also rapidly release medicines after entering tumor cells to induce cell apoptosis. The system has the advantages that the process is simple, the carrier biocompatibility is good, the enrichment of medicines at brain tumor parts is remarkably improved, and the concentration of medicines in brain tumor cells is increased. By means of the system, medicines can be conveyed to brain glioma selectively and efficiently.
Owner:SUZHOU UNIV
Preparation method of feed-grade zinc oxide premixing agent
InactiveCN107080055AHigh chemical activityImprove absorption rateAccessory food factorsWorking-up animal fodderSilanesAtmospheric temperature
The invention provides a preparation method of a feed-grade zinc oxide premixing agent. The preparation method comprises the following steps of (1) grinding zinc oxide powder, performing screening with an 80-mesh sieve to obtain fine powder, adding the fine powder into an ethanol water solution, dropwise adding a silane coupling agent solution, and taking suspension liquid for standby application; (2) adding a delta-tocopherol-ethanol solution to the suspension liquid under the normal atmospheric temperature, performing heating to 60-70 DEG C, performing a reaction for 2-3 hours, taking suspension liquid, performing filtering, washing filter cakes with ethanol, performing drying, performing grinding, and performing screening so as to obtain nanometer zinc oxide; (3) mixing the nanometer zinc oxide with starch, sodium hydroxypropycellulose, sodium cyclamate, a binder and a wetting agent through a mixing machine, performing granulation, performing shot blasting, and performing drying to obtain mini pills; and (4) performing screening on the mini pills with a classifying sieve so as to obtain the feed-grade zinc oxide premixing agent of which the particle size is 20-80 meshes. The zinc oxide particles in the made zinc oxide premixing agent are dispersed in a nanometer grade, so that the feed-grade zinc oxide premixing agent is uniform to disperse and not liable to agglomerate, has favorable biological value and utilization, and cannot generate side effects on growth of animals.
Owner:ZHEJIANG ESIGMA BIOTECH CO LTD
Use of single targeted reduction-responsive vesicle nano-drug in preparation of drug for treating brain tumors
ActiveCN108126210AHigh drug loading efficiencyIncreased release ratePharmaceutical non-active ingredientsAntineoplastic agentsBlood vesselGlioma cell
The invention discloses a use of a single targeted reduction-responsive vesicle nano-drug in preparation of a drug for treating brain tumors. Through reduction-responsive reversibly crosslinked vesicles of block polymers such as PEG-P(TMC-DTC), PEG-P(LA-DTC), PEG-P(TMC-DTC)-PEI, PEG-P(LA-DTC)-PEI, PEG-P(TMC-DTC)-Sp and PEG-P(LA-DTC)-Sp and a targeting polymer using ApoE as a targeting molecule, small-molecule chemotherapeutic drugs, protein drugs and gene drugs sensitive to brain glioma cells are efficiently encapsulated. The drug-carrying targeting vesicles can efficiently target multiple receptors (including LRP-1, LRP-2 and LDLR) that are highly expressed on the surfaces of brain microvascular endothelial cells in tumor regions thereby efficiently penetrating the blood-brain barrier andbeing efficiently enriched in the brain tumor region. ApoE-targeting related receptors are also highly expressed on the surfaces of glioma cells so that the drug-carrying targeting vesicles can be efficiently endocytosed by glioma cells and fast release drugs to induce apoptosis.
Owner:SUZHOU UNIV
Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method
ActiveCN113926432AEnhanced inhibitory effectReduce false negative rateOther chemical processesAlkali metal oxides/hydroxidesLysisMagnetic bead
Owner:HANGZHOU ANYU TECH CO LTD
Microsphere for double protection of antibody drug and intravitreal injection, and preparation method thereof
ActiveCN108938600AGood denaturation effectPromote aggregationSenses disorderAntibody ingredientsSide effectMicrosphere
The invention provides a microsphere for double protection of antibody drug and intravitreal injection, and a preparation method thereof. Particles of a monoclonal antibody drug are prepared by adopting a method of water phase-water phase emulsification, polylactic acid-glycolic acid copolymer and polyketal are utilized as carrier materials, and a sustained release microsphere that wraps and carries the particles of a dextran-monoclonal antibody drug is prepared by employing an emulsion solvent volatilization method of water phase-oil phase-solid phase. Denaturation and aggregation of antibodies are caused by an acidic microenvironment of polylactic acid-glycolic acid in an organic phase / aqueous phase interface and a degradation process, the drug loading capacity of the microsphere is increased and a burst release phenomenon during a releasing process of the microsphere is reduced, and double protection on the monoclonal antibody drug of the microsphere is achieved; the polyketal enables the drug loading capacity of the microsphere to be increased, enables the stimulation of an acidic degradation product of the polylactic acid and glycolic acid on eye environment to be reduce, andenables side effects such as endophthalmitis to be reduced; and the microsphere can release the monoclonal antibody for not less than 28 days in the in vitro environment and for not less than 2 monthsin the eye, and so, the frequency of administration can be reduced, pain of patients and economic burden are reduced, and the compliance of patients is improved.
Owner:南京锐利施生物技术有限公司
A kind of all-effect eye cream and preparation method thereof
ActiveCN106580798BNo smellGood chemical stabilityCosmetic preparationsToilet preparationsWrinkle skinLanosterol
The invention discloses an all-in-one eye cream and a preparation method. The eye cream comprises deionized water, cis-butanediol, hydrogenated polyisobutene, dicaprylyl carbonate, isononyl isononanoate, glycerol, polydimethylsiloxane, dimethiconol, mycose, bio-saccharide gum-1, cetearyl alcohol olivate, sorbitan olivate, palmitoyl tripepitde-5, shaddock extract, Equisetum arvense extract, ascorbyl methylsilanol pectinate, methylsilanol hydroxyproline aspartate, dimethylsilanol hyaluronate, cetostearyl alcohol, cetearyl glucoside, C10-30 cholesterol / lanosterol esters, shea butter, Dipalmitoyl Hydroxyproline, sodium acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-80, isocetane, phenoxyethanol and the like. The eye cream can solve a variety of eye skin problems such as periorbital puffiness, black rim of eye and eye wrinkles.
Owner:广东科盈科技有限公司
Swab sample preserving fluid
PendingCN113322252ARapid and efficient inactivationAvoid secondary infectionDNA preparationSucroseViral nucleic acid
The invention discloses a swab sample preserving fluid. The swab sample preserving fluid is prepared from the components of isosorbide dimethyl ether, sucrose fatty acid ester and polyether NPE-108. The preserving liquid is a swab sample preserving liquid which can rapidly inactivate viruses and is good in preservation effect, can preserve viral nucleic acid for 16 days at the normal temperature of 37 DEG C and can preserve viral nucleic acid for 60 days at the temperature of 2-8 DEG C, can effectively protect nucleic acid from being degraded, does not affect subsequent extraction and detection of nucleic acid, and is safe and effective. Moreover, the swab sample preserving liquid can be used for quickly inactivating the preserved virus sample within 10 minutes, so that secondary infection of an operator is effectively prevented.
Owner:GUANGZHOU HYBRIBIO MEDICINE TECH LTD +2
Application of rice osbbti4 protein gene in improving rice blast resistance
ActiveCN108517333BIncrease resistanceImprove disease resistanceProtease inhibitorsFermentationBiotechnologyNucleotide
The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Hepatitis B virus serum sample preserving diluent and preparation method thereof
InactiveCN108998569AUndamagedFew ingredientsMicrobiological testing/measurementMicroorganism based processesSerum igeSerum samples
The invention discloses a hepatitis B virus serum sample preserving diluent. The hepatitis B virus serum sample preserving diluent comprises Trise-HCL, NaCL, BSA, gentamicin sulfate, P-300, sunset yellow, lemon yellow and deionized purified water. On the basis of 1 L of the deionized purified water, the contents of Trise-HCL, NaCL, BSA, gentamicin sulfate, P-300, sunset yellow and lemon yellow are0.01 mol, 8.8 g, 20 g, 0.68 ml, 2.0 ml, 0.01 g, and 0.01 g, respectively. The hepatitis B virus serum sample preserving diluent of the invention has the advantages that the diluent comprises few components; the preparation method is simple; the prepared finished preserving diluent can protect the structure of a viral nucleic acid material from damage, inhibit the activity of DNase and effectivelyprotect the DNAs of a virus from degradation, has the advantages of stability, easy storage, convenience, etc., and is applicable to preservation and dilution of the hepatitis B virus (HBV DNA) serumsample. Test results prove that after addition of the preserving diluent prepared by the invention into the hepatitis B virus (HBV DNA) serum sample, DNAs and RNAs can be completely stored at 37 DEGC for 1 week, stored at 20-25 DEG C for 1 month, stored at 4 DEG C for 6 months and store at -20 DEG C or -80 DEG C for a long time.
Owner:AUTOBIO DIAGNOSTICS CO LTD
Oxygen-based high-efficiency clean bleaching pulp preparation method and device thereof
ActiveCN109267413AEnhanced mass transferConsistent mass transferPulp bleachingHigh concentrationChemical reaction
The invention discloses an oxygen-based high-efficiency clean bleaching pulp preparation method and a device thereof. A hollow hybrid rotor in the device is connected to the shaft end of a high-speedvariable frequency motor spindle located at the bottom of a mixing reaction chamber by bolts. Blades of the hollow hybrid rotor are a plurality of linear or spiral blades distributed symmetrically ina hollow structure. The method is widely used in oxygen bleaching or ozone bleaching of chemical pulp of any raw material. All use oxygen-containing bleaching agents. Final products decomposed from the chemical reaction are oxygen and water, and the residual of harmful substances in the bleached pulp is eliminated. The bleached pulp is high in whiteness, more uniform and not prone to return to yellow. A variable frequency motor drives the hollow rotor to conduct high speed mixing through the spindle and a mechanical seal, so that medium- and high-concentration pulp and an oxygen-based gaseousbleaching agent in the mixed reaction chamber are more efficient in mass transfer. The mixed contact is more uniform and sufficient. The device is reasonable in structure, can accurately control consumption of liquid, oxygen and ozone and simple and efficient in the bleached pulp preparation process.
Owner:SOUTH CHINA UNIV OF TECH
Nanoparticle containing 5-methylpyrimidine-2, 4 (1H, 3H)-diketone derivative and preparation method and application thereof
PendingCN114601922AEfficient releaseAchieve therapeutic effectPowder deliveryOrganic active ingredientsDiketoneNanoparticle
The invention relates to nanoparticles as well as a preparation method and application thereof, and belongs to the field of biological medicines. The nanoparticle comprises a 5-methylpyrimidine-2, 4 (1H, 3H)-diketone derivative and an auxiliary material. The nanoparticles can entrap nucleic acid, and have the advantages of low toxicity, high entrapment efficiency, good transfection effect and good bioavailability. The preparation method is simple to operate, low in cost, environment-friendly and beneficial to industrial production.
Owner:SHENZHEN LONGUIDE BIOPHARMA CORP
Preparation method of chitosan derivative nanoparticles for delivering siRNA
ActiveCN111840575ADecreased expression of β-cateninEffective internalizationOrganic active ingredientsPowder deliveryCancer cellColon cancer cell
The invention relates to a preparation method of chitosan derivative nanoparticles for delivering siRNA, and belongs to the technical field of gene biology. A positively charged chitosan hydrochloridesolution can be mixed with an siRNA solution capable of inhibiting protein expression related to proliferation and migration of colon cancer cells, then a carboxymethyl chitosan solution with negative electricity is added, and self-assembly is performed to form chitosan derivative nanoparticles (siRNA-CDNPs) for delivering siRNA through electrostatic interaction between molecules. The chitosan derivative nanoparticles for delivering the siRNA can effectively protect the siRNA under the condition that the pH value is 1.2, and under the condition that the pH value of a colon cancer cell environment is 5.5, controllable release of the siRNA is realized through response to external stimulation (ultrasound); the siRNA-CDNPs can effectively inhibit the expression of beta-catenin protein in colon cancer cells, and is expected to inhibit the proliferation and migration of cancer cells. The preparation method is easy to operate, green, environment-friendly and low in cost.
Owner:HEFEI UNIV OF TECH
Amphiphilic polypeptide P13 and preparation method thereof
ActiveCN108752429AThe role of apparent active recognition bindingImprove buffering effectPeptide preparation methodsCrystallographyCancer cell
The invention discloses amphiphilic polypeptide P13 and a preparation method thereof and belongs to the field of polypeptide. The amphiphilic polypeptide disclosed by the invention has amino acid sequences with hydrophilic segments DGRHHH and lyophobic segments, the lyophobic segments consist of L and A amino acids, and specifically, the amino acid sequences are DGRHHHLLLAAAA. The invention further provides a method for preparing the amphiphilic polypeptide P13. The method is a solid phase synthesis method. The amphiphilic polypeptide P13 disclosed by the invention has active targeting recognition upon cancer cells, meanwhile has a sponge proton effect, that is, an escape capability, and has great values in development and application of drug delivery carriers.
Owner:ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
Active targeting type amphiphilic polypeptide composite nano-micelle prodrug as well as preparation and application thereof
ActiveCN113069416AGood biocompatibilityNot easy to degradePhotodynamic therapyPharmaceutical non-active ingredientsArginineSide chain
The invention discloses an active targeting type amphiphilic polypeptide composite nano-micelle prodrug as well as preparation and application thereof, and belongs to the technical field of biological medicines. The composite nano-micelle prodrug is obtained by co-assembling a first polypeptide with negative charges and a second polypeptide with positive charges through electrostatic interaction and hydrophilic and hydrophobic effects; the first polypeptide is Lys-AAm-Gly-Arg-Gly-Asp-Ser, wherein AA is aspartic acid or glutamic acid, and m is 1, 2, 3 or 4; the second polypeptide is Cys-BBn, wherein BB is lysine, arginine or histidine, and n is 1, 2, 3 or 4; amino at a N end of the first polypeptide is connected with a hydrophobic alkyl chain, and amino at a lysine side chain of the first polypeptide is connected with a fluorescent molecule; cysteine in the second polypeptide is covalently linked with an anti-tumor drug through sulfydryl. The nano-composite micelle can be actively targeted to tumor cells, and is disassembled after responding to a tumor slightly acidic environment to form a small-size micelle with a size of 20-30 nm, so that deep delivery of the polypeptide anti-tumor prodrug at a tumor part is facilitated.
Owner:HUAZHONG UNIV OF SCI & TECH
Flame-retardant waterproof exterior wall paint coating
InactiveCN112391096AWith waterproof functionEase of ring-opening polymerizationFireproof paintsWax coatingsEpoxyChlorinated paraffins
The invention discloses a flame-retardant waterproof exterior wall paint coating, which is prepared from the following raw materials by weight percentage: 55-75% of epoxy resin, 5-8% of a styrene-acrylic emulsion, 3-6% of a phosphorus-containing waterproof agent, 5-10% of talcum powder, 0.5-1.5% of chlorinated paraffin, 2-5% of polyether polyol, 4-9% of vinyl acetate, 0.3-0.8% of a defoaming agentand 5-10% of ethanol. By preparing the phosphorus-containing waterproof agent with waterproof and flame-retardant properties and connecting the phosphorus-containing waterproof agent into the epoxy resin, the phosphorus-containing waterproof agent and the epoxy resin are firmly connected together through ring-opening polymerization and chemical bonds, and in the preparation process of the exterior wall paint coating, the situation that the waterproof agent or the flame retardant is mixed unevenly does not exist; and when a coating film is prepared, the situation of non-uniform waterproof andflame-retardant properties is prevented, and meanwhile, when the coating is eroded by rainwater and sunlight, the waterproof and flame-retardant components of the coating cannot be broken, so that theaging of the exterior wall paint is effectively prevented, and the service life of the exterior wall paint is prolonged.
Owner:石鑫
Compound lonicera and forsythia ammonia-sensitive capsules and preparation technology thereof
ActiveCN102657787AReduce consumptionLong validity periodOrganic active ingredientsAntiviralsVitamin CQuality performance
The invention discloses compound lonicera and forsythia ammonia-sensitive capsules. The capsules comprise polyacrylic resin II, vitamin C, citric acid, paracetamol, chlorpheniramine maleate, dry lonicera and forsythia paste, starch, weeping forsythia volatile oil, schizonepeta volatile oil, mint oil and beta cyclodextrin. The invention also discloses a preparation technology for the capsules. The technology comprises the following steps of: (A), preparing the dry lonicera and forsythia paste; (B), weighing raw materials; (C), dissolving the polyacrylic resin II; (D), coating the vitamin C and the citric acid with resin liquid; (E), coating the paracetamol with the resin liquid; (F), mixing the chlorpheniramine maleate, the dry lonicera and forsythia paste and the starch; (G), coating the weeping forsythia volatile oil, the schizonepeta volatile oil and the mint oil with the beta cyclodextrin; and (H), putting the obtained fine powder into a mixer, adding the coated weeping forsythia volatile oil, schizonepeta volatile oil and mint oil, mixing and packing. The invention has the advantages that stable content of the raw materials is ensured, the validity period of the capsules is prolonged, the production efficiency is improved, the quality performance of the capsules is stable, and the yield of the capsules is improved.
Owner:四川彩虹制药有限公司
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