71results about How to "Protection from degradation" patented technology

The invention relates to a tumor-targeted gene vector material and a preparation method and use thereof. In the invention, functional graphene oxide is used as a targeted transport vector of genomic molecules such as siRNA. The preparation method comprises: connecting folic acid tumor cell targeted molecules and amino-terminated polyethylene glycol by amido bonds; connecting the graphene oxide with the coupling material by amido bonds to obtain the tumor targeted functional graphene oxide; anchoring aminated molecules with an aromatic conjugated structure onto the surface of the graphene oxide through pi-pi conjugation; and finally, preparing the tumor targeted high-efficiency gene transport system by static attraction between the positively charged functional graphene oxide and the negatively charged genomic molecules. The vector material has a high targeting performance for tumor cells in which folic acid receptor is expressed more highly, and after being loaded with siRNA, the corresponding target gene level and related protein expression can be reduced. The invention provides a new method for the in-vivo targeted transportation of genes.

The invention discloses an environmental protection high efficiency foaming agent for shield construction. The foaming agent comprises the following components in percentage by weight: 5 to 20 percent of aliphatic alcohol polyethenoxy ether sulfate, 1 to 8 percent of alcohol amide, 0.5 to 8 percent of alcohol ether, and 64 to 93.5 percent of water. The foaming agent for shield construction has a high foaming ratio and produces abundant and fine foams with even diameter distribution. The produced bubbles have small pores, good lubricity and stability, simple production operation, and lower cost. The foaming agent can better meet the requirements of the slag soil improvement during the process of earth pressure balanced shield.

The invention relates to the field of biology, and relates to a method of extracting a nucleic acid by taking sputum as a sample and application of the extraction method. The invention provides a dedicated kit for extracting a nucleic acid from sputum and an extraction method. The specific extraction method comprises the following steps: adding a digestive juice KB which is 1-5 times of the sputum into the sputum, vibrating and uniformly mixing the liquid, adding protease to the liquid, keeping warm at the temperature of 37-65 DEG C for 1-5 hours, adding a binding solution BS which is 0.5-3 times of the sputum sample capacity, after vibrating and uniformly mixing, enabling the complete solution to pass through a nucleic acid adsorption column, allowing the nucleic acid in the sample to be bound with a silica membrane of the adsorption column, conducting steps of washing, eluting and the like, and extracting the nucleic acid from the sputum. The nucleic acid extracted by the kit provided by the invention is high in yield and purity, and can be directly used for molecular biological detection.

The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.

The invention discloses application of a reduction response polymersome nano-drug in preparation of brain tumor treatment drugs. Block copolymers PEG-P(TMC-DTC), PEG-P(LA-DTC), PEG-P(TMC-DTC)-PEI, PEG-P(LA-DTC)-PEI, PEG-P(TMC-DTC)-Sp, PEG-P(LA-DTC)-Sp and targeted polymers thereof are combined in different ratios, and dual-targeted reduction-sensitive reversible crosslinked vesicles of different targeted molecular proportions can be prepared. Through the hydrogen-bond interaction or other electrostatic interaction between great hydrophilic inner cavities of the crosslinked vesicles and/or PEI-spermine and protein drugs or gene drugs, efficient encapsulation of micromolecular chemotherapeutic drugs, the protein drugs and the gene drugs can be achieved. By means of the in-vivo dual-targeteddrug-carrying crosslinked vesicles, more efficient blood brain barrier crossing, higher tissue penetration depth and endocytosis of more glioma cells can be achieved, and the reduction response polymersome nano-drug is very potential in treating brain gliomas and efficient.

The invention discloses a method for extracting and transforming excrement DNA in quantity. The method comprises the steps of collecting 1g-5g of excrement into a tube containing protecting liquid, adding cracking liquid for adequately cracking so as to remove an inhibitor, precipitating nucleic acid by virtue of an organic solvent, resuspending precipitates so as to obtain nucleic acid for nucleic acid extraction or nucleic acid transformation, carrying out a series of combined washing steps, and finally carrying out elution, so as to obtain excrement DNA. The method comprises the steps of extraction and transform of excrement DNA and combination of extraction and transformation. The method has the characteristics of low cost, high utilization rate, good repeatability, simplicity and rapidness; a large number of excrement DNA or transformed excrement DNA can be obtained, and more possibilities can be provided for the transformation or the downstream detection.

The invention relates to a fecal nucleic acid preserving fluid as well as a preparation method and application thereof. The fecal nucleic acid preserving solution comprises a Tris-HCl buffer solutionwith the concentration being 0.01 to 1M, ethylenediaminetetraacetic acid (EDTA) with the concentration being 0.01 to 1M, sodium chloride with the concentration being 0.01 to 0.5 M, sodium citrate withthe concentration being 0.01 to 0.5 M and absolute ethyl alcohol with the volume concentration being 10 to 30 percent, wherein the pH value of the preservation solution is 7.0 to 10.0. The fecal nucleic acid preserving fluid provided by the invention can effectively maintain the stability of nucleic acid in feces, and is convenient for a user to sample at any time and transport at normal temperature. And downstream detection of intestinal flora is not influenced.

The invention discloses a preparation method of high-purity 2-alkyl-4-isothiazoline-3-ketone. The preparation method comprises the following steps: suspending amide into a reaction medium inert solvent; introducing CMI and MIT mixture tail gas or MIT tail gas generated by the previous step, so as to obtain an amide suspending solution with proper hydrogen chloride content; and introducing a chlorinating reagent. According to the preparation method, a byproduct, namely hydrogen chloride, has an inhibition effect on the generation of 5-chloro-2-alkyl-4-isothiazoline-3-ketone. The content of the hydrogen chloride is increased through utilizing reaction raw materials and solvents; and finally, the high-purity 2-alkyl-4-isothiazoline-3-ketone is obtained.

The invention discloses a preparation process of environment-friendly water-based ink. The preparation process comprises the following steps: step 1, weighing raw materials; step 2, adding deionized water into a dispersion cylinder, then adding a pigment, carrying out ultrasonic treatment at normal temperature for 10 minutes to prepare an aqueous dispersion liquid of the pigment, then adding a wetting agent and half of a dispersing agent, and carrying out high-speed dispersion and grinding to obtain color paste; and step 3, mixing the color paste with the polyacrylate emulsion, and adding a modified filler, a dispersing agent and a defoaming agent to obtain the environment-friendly water-based ink. According to the invention, the water-based polyacrylate emulsion is used as a film-formingsubstance of the ink; the emulsion has high film-forming property and adhesive force; by adding the modified filler, the phenomenon of ink aging caused by ultraviolet rays can be effectively prevented, the damage caused by the ultraviolet rays to an organic resin base material is reduced, and the water-based ink has water resistance and ultraviolet-resistant stability at the same time; the raw materials are green and environment-friendly, and the water-based ink which is environment-friendly, excellent in comprehensive performance and capable of effectively defending ultraviolet rays is obtained.

The invention discloses application of targeted reduction response vesicular nanometer medicines in preparation of brain tumor treatment medicines. Reduction sensitive reversible crosslinking vesiclesbased on block polymers PEG-P(TMC-DTC), PEG-P(LA-DTC), PEG-P(TMC-DTC)-PEI, PEG-P(LA-DTC)-PEI, PEG-P(TMC-DTC)-Sp or PEG-P(LA-DTC)-Sp and targeting polymers with ANG as the targeting molecules can efficiently wrap small-molecular chemotherapy medicines, protein medicines and gene medicines sensitive to brain glioma cells. The medicine-carrying vesicles can efficiently permeate through a blood brainbarrier in vivo to enter tumor substances and can also rapidly release medicines after entering tumor cells to induce cell apoptosis. The system has the advantages that the process is simple, the carrier biocompatibility is good, the enrichment of medicines at brain tumor parts is remarkably improved, and the concentration of medicines in brain tumor cells is increased. By means of the system, medicines can be conveyed to brain glioma selectively and efficiently.

The invention provides a preparation method of a feed-grade zinc oxide premixing agent. The preparation method comprises the following steps of (1) grinding zinc oxide powder, performing screening with an 80-mesh sieve to obtain fine powder, adding the fine powder into an ethanol water solution, dropwise adding a silane coupling agent solution, and taking suspension liquid for standby application; (2) adding a delta-tocopherol-ethanol solution to the suspension liquid under the normal atmospheric temperature, performing heating to 60-70 DEG C, performing a reaction for 2-3 hours, taking suspension liquid, performing filtering, washing filter cakes with ethanol, performing drying, performing grinding, and performing screening so as to obtain nanometer zinc oxide; (3) mixing the nanometer zinc oxide with starch, sodium hydroxypropycellulose, sodium cyclamate, a binder and a wetting agent through a mixing machine, performing granulation, performing shot blasting, and performing drying to obtain mini pills; and (4) performing screening on the mini pills with a classifying sieve so as to obtain the feed-grade zinc oxide premixing agent of which the particle size is 20-80 meshes. The zinc oxide particles in the made zinc oxide premixing agent are dispersed in a nanometer grade, so that the feed-grade zinc oxide premixing agent is uniform to disperse and not liable to agglomerate, has favorable biological value and utilization, and cannot generate side effects on growth of animals.

The invention provides a microsphere for double protection of antibody drug and intravitreal injection, and a preparation method thereof. Particles of a monoclonal antibody drug are prepared by adopting a method of water phase-water phase emulsification, polylactic acid-glycolic acid copolymer and polyketal are utilized as carrier materials, and a sustained release microsphere that wraps and carries the particles of a dextran-monoclonal antibody drug is prepared by employing an emulsion solvent volatilization method of water phase-oil phase-solid phase. Denaturation and aggregation of antibodies are caused by an acidic microenvironment of polylactic acid-glycolic acid in an organic phase/aqueous phase interface and a degradation process, the drug loading capacity of the microsphere is increased and a burst release phenomenon during a releasing process of the microsphere is reduced, and double protection on the monoclonal antibody drug of the microsphere is achieved; the polyketal enables the drug loading capacity of the microsphere to be increased, enables the stimulation of an acidic degradation product of the polylactic acid and glycolic acid on eye environment to be reduce, andenables side effects such as endophthalmitis to be reduced; and the microsphere can release the monoclonal antibody for not less than 28 days in the in vitro environment and for not less than 2 monthsin the eye, and so, the frequency of administration can be reduced, pain of patients and economic burden are reduced, and the compliance of patients is improved.

The invention discloses an all-in-one eye cream and a preparation method. The eye cream comprises deionized water, cis-butanediol, hydrogenated polyisobutene, dicaprylyl carbonate, isononyl isononanoate, glycerol, polydimethylsiloxane, dimethiconol, mycose, bio-saccharide gum-1, cetearyl alcohol olivate, sorbitan olivate, palmitoyl tripepitde-5, shaddock extract, Equisetum arvense extract, ascorbyl methylsilanol pectinate, methylsilanol hydroxyproline aspartate, dimethylsilanol hyaluronate, cetostearyl alcohol, cetearyl glucoside, C10-30 cholesterol/lanosterol esters, shea butter, Dipalmitoyl Hydroxyproline, sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, isocetane, phenoxyethanol and the like. The eye cream can solve a variety of eye skin problems such as periorbital puffiness, black rim of eye and eye wrinkles.

The invention discloses a swab sample preserving fluid. The swab sample preserving fluid is prepared from the components of isosorbide dimethyl ether, sucrose fatty acid ester and polyether NPE-108. The preserving liquid is a swab sample preserving liquid which can rapidly inactivate viruses and is good in preservation effect, can preserve viral nucleic acid for 16 days at the normal temperature of 37 DEG C and can preserve viral nucleic acid for 60 days at the temperature of 2-8 DEG C, can effectively protect nucleic acid from being degraded, does not affect subsequent extraction and detection of nucleic acid, and is safe and effective. Moreover, the swab sample preserving liquid can be used for quickly inactivating the preserved virus sample within 10 minutes, so that secondary infection of an operator is effectively prevented.

The invention discloses an oxygen-based high-efficiency clean bleaching pulp preparation method and a device thereof. A hollow hybrid rotor in the device is connected to the shaft end of a high-speedvariable frequency motor spindle located at the bottom of a mixing reaction chamber by bolts. Blades of the hollow hybrid rotor are a plurality of linear or spiral blades distributed symmetrically ina hollow structure. The method is widely used in oxygen bleaching or ozone bleaching of chemical pulp of any raw material. All use oxygen-containing bleaching agents. Final products decomposed from the chemical reaction are oxygen and water, and the residual of harmful substances in the bleached pulp is eliminated. The bleached pulp is high in whiteness, more uniform and not prone to return to yellow. A variable frequency motor drives the hollow rotor to conduct high speed mixing through the spindle and a mechanical seal, so that medium- and high-concentration pulp and an oxygen-based gaseousbleaching agent in the mixed reaction chamber are more efficient in mass transfer. The mixed contact is more uniform and sufficient. The device is reasonable in structure, can accurately control consumption of liquid, oxygen and ozone and simple and efficient in the bleached pulp preparation process.

The invention relates to a preparation method of chitosan derivative nanoparticles for delivering siRNA, and belongs to the technical field of gene biology. A positively charged chitosan hydrochloridesolution can be mixed with an siRNA solution capable of inhibiting protein expression related to proliferation and migration of colon cancer cells, then a carboxymethyl chitosan solution with negative electricity is added, and self-assembly is performed to form chitosan derivative nanoparticles (siRNA-CDNPs) for delivering siRNA through electrostatic interaction between molecules. The chitosan derivative nanoparticles for delivering the siRNA can effectively protect the siRNA under the condition that the pH value is 1.2, and under the condition that the pH value of a colon cancer cell environment is 5.5, controllable release of the siRNA is realized through response to external stimulation (ultrasound); the siRNA-CDNPs can effectively inhibit the expression of beta-catenin protein in colon cancer cells, and is expected to inhibit the proliferation and migration of cancer cells. The preparation method is easy to operate, green, environment-friendly and low in cost.

The invention discloses an active targeting type amphiphilic polypeptide composite nano-micelle prodrug as well as preparation and application thereof, and belongs to the technical field of biological medicines. The composite nano-micelle prodrug is obtained by co-assembling a first polypeptide with negative charges and a second polypeptide with positive charges through electrostatic interaction and hydrophilic and hydrophobic effects; the first polypeptide is Lys-AAm-Gly-Arg-Gly-Asp-Ser, wherein AA is aspartic acid or glutamic acid, and m is 1, 2, 3 or 4; the second polypeptide is Cys-BBn, wherein BB is lysine, arginine or histidine, and n is 1, 2, 3 or 4; amino at a N end of the first polypeptide is connected with a hydrophobic alkyl chain, and amino at a lysine side chain of the first polypeptide is connected with a fluorescent molecule; cysteine in the second polypeptide is covalently linked with an anti-tumor drug through sulfydryl. The nano-composite micelle can be actively targeted to tumor cells, and is disassembled after responding to a tumor slightly acidic environment to form a small-size micelle with a size of 20-30 nm, so that deep delivery of the polypeptide anti-tumor prodrug at a tumor part is facilitated.