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Swab sample preserving fluid

A technology for preserving liquid and samples, applied in the field of nucleic acid detection, which can solve the problems of unknown inactivation and preservation effect of new coronavirus, no virus inactivation function, and inability to effectively prevent secondary infection of operators.

Pending Publication Date: 2021-08-31
GUANGZHOU HYBRIBIO MEDICINE TECH LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent CN 111925941A discloses a virus preservation solution and its application. In the virus preservation solution, although the virus nucleic acid can be preserved for up to 21 days, the preservation solution does not have the virus inactivation function and cannot effectively prevent the operator from secondary secondary infection
Chinese patent CN 111718908A discloses a virus sample preservation solution and its preparation method and application. The virus sample preservation solution can inactivate the virus for 10 minutes. The preservation time of the conditions is 1-2 months, and long-term preservation can be achieved at -20°C, but this preservation solution has not been used for the preservation of COVID-19, and its inactivation and preservation effects on the new coronavirus are unknown

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 preservation solution

[0033] 1. After a lot of explorations and attempts, the present invention has obtained a preservation solution formula with good virus inactivation effect and long nucleic acid storage time, and this formula is used as the official formula.

[0034] The formula of preservation solution 1 is shown in Table 1 (official formula), and each component is dissolved in ddH 2 O and set the volume to 1L, shake and mix well, and the pH of the preservation solution is 7±0.5.

[0035] Table 1 Preservation solution 1 (official formula, 1000mL)

[0036]

[0037] Note: The concentration calculation method is w / w for solids and v / v for liquids.

[0038] In addition, some failed formulations are shown below for comparison.

[0039] Preservation solution 2 formula (as shown in Table 2, failure formula): The difference between this formula and the official formula is that polyether NPE-108, sucrose fatty acid ester, and isosorbi...

Embodiment 2

[0052] Example 2 The investigation of the preservation solution on the preservation time and preservation effect of the new coronavirus pseudovirus

[0053] 1. Use the four preservation solution formulations in Example 1 as experimental materials to verify their preservation time for viruses.

[0054] (1) Study on storage time at 37°C

[0055] Test sample: Dilute the new coronavirus to 10% with the above preservation solution. 4 Copies / mL, stored at 37°C for 0 days, 2 days, 5 days, 8 days, 12 days, 14 days, and 16 days, and then extract the virus with the Kaipu new coronavirus 2019-nCoV nucleic acid detection kit (fluorescent PCR method) Nucleic acid, and detection.

[0056] The test results are shown in Table 5. The samples stored in the preservation solution 1 of the present invention for 16 days at 37°C can still be detected by the new crown kit, while the other samples stored in the preservation solution for 16 days cannot be detected by the new coronavirus 2019-nCoV. Nuc...

Embodiment 3

[0072] The collection preservation and detection of embodiment 3 swab samples

[0073] 1. Nasopharyngeal swab collection

[0074] 1) The patient's head is tilted back (about 70 degrees) and kept still.

[0075] 2) Estimate the distance from the base of the ear to the nostril with a swab stick.

[0076] 3) Insert from the nostril perpendicular to the face, and the penetration distance should be at least half of the length from the earlobe to the tip of the nose. After encountering resistance, it reaches the posterior nasopharynx. It should stay for a few seconds to absorb the secretions (generally 15-30 seconds), and rotate the swab 3-5 times. It is advisable to gently rotate the swab to take it out. The swab head is immersed in the preservation liquid. in the collection tube.

[0077] 4) Break off the sterile swab rod at the top, discard the tail, tighten the tube cap and seal it with parafilm.

[0078] 2. Oropharyngeal swab collection

[0079] 1) Ask the patient to rinse...

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Abstract

The invention discloses a swab sample preserving fluid. The swab sample preserving fluid is prepared from the components of isosorbide dimethyl ether, sucrose fatty acid ester and polyether NPE-108. The preserving liquid is a swab sample preserving liquid which can rapidly inactivate viruses and is good in preservation effect, can preserve viral nucleic acid for 16 days at the normal temperature of 37 DEG C and can preserve viral nucleic acid for 60 days at the temperature of 2-8 DEG C, can effectively protect nucleic acid from being degraded, does not affect subsequent extraction and detection of nucleic acid, and is safe and effective. Moreover, the swab sample preserving liquid can be used for quickly inactivating the preserved virus sample within 10 minutes, so that secondary infection of an operator is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection. More specifically, it relates to a swab sample preservation solution. Background technique [0002] Swab sampling is a common method for pathogen detection and sampling. For example, in the detection and screening of infected persons under the novel coronavirus pneumonia epidemic, a large number of swab samples need to be collected for detection. The preservation and transportation of swab samples requires the use of swab sample preservation solution, that is, virus preservation solution, which is suitable for the collection, preservation and transportation of common virus samples such as new coronavirus, influenza virus, hand-foot-mouth virus, etc. A liquid that protects the detected substance of the virus from sub-virus samples. It can save the collected throat swabs, nasal swabs or tissue samples from specific parts. The stored samples can be used for subsequent molecular biolo...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 葛毅媛朱兴彪郭津津谢龙旭王博管亚伟冯文华
Owner GUANGZHOU HYBRIBIO MEDICINE TECH LTD
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