Decontamination of surfaces contaminated with prion-infected material with oxidizing agent-based formulations

A technology of prions and oxidants, applied in the direction of detergent materials, detergent compounding agents, chemical instruments and methods, etc., can solve the problems of damage to medical equipment, damage, etc., and achieve the effect of alleviating the effect of equipment

Inactive Publication Date: 2005-02-23
AMERICAN STERILIZER CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, such handling damages most medical devices and causes damage in other ways

Method used

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  • Decontamination of surfaces contaminated with prion-infected material with oxidizing agent-based formulations
  • Decontamination of surfaces contaminated with prion-infected material with oxidizing agent-based formulations
  • Decontamination of surfaces contaminated with prion-infected material with oxidizing agent-based formulations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Samples were prepared by treating it with a prion model (BSA denatured protein). The samples were then treated with a composition containing peracetic acid at a concentration of 1,000 mg / L at 40-60°C. The composition also includes a surfactant system, a phosphate buffer system, an organic corrosion inhibitor, and a chelating agent. The composition is free of heavy metals.

[0069] Table 4 summarizes the amount of BSA decomposed during 12 minutes of exposure to the composition.

[0070] temperature(℃)

[0071] The results showed that at 40°C and 45°C, little, if any, protein breakdown occurred. Whereas, at 50°C and 55°C, proteins were efficiently decomposed. At 60°C, some protein residue remained, suggesting protein clumping. That is, when the temperature is around and above 60°C, the protein aggregates and has a higher resistance to protect itself from attack by peracetic acid or other strong oxidants.

[0072] The effect of peracetic acid concentration o...

Embodiment 2

[0076] Ileal fluid-dependent organism (IFDO) form prion model specimens were treated with peracetic acid formulations as described in Example 1 at 50°C with peracetic acid concentrations of 0-2,000 mg / liter, and then in modified mycoplasma cultured in liquid medium. Aliquots of the IFDO suspension were added directly to 1,000 mg / liter, 1,500 mg / liter, 2,000 mg / liter, and 2,500 mg / liter peracetic acid solutions at 50°C. The models were then incubated in modified Mycoplasma liquid medium for 48 hours at 37°C. Figure 5 Shown is the logarithm versus time curve for the prion model in solutions of 0, 1,000, 1,500, 2,000, and 2,500 mg / liter. An initial concentration of 2,500 mg / liter of peracetic acid is preferred since high concentrations of material on practical devices can decompose peracetic acid.

Embodiment 3

[0078] Specimens of the prion model of ileal fluid-dependent organism (IFDO) morphology were treated with 1,500 mg / L peracetic acid formulation at various temperatures between 25-60°C and then cultured in modified mycoplasma-based agar such as as described in Example 2. Image 6 The relationship between the logarithmic decline of the prion model and the time is described when the temperature is 25, 30, 40, 45, 50, 55, and 60 °C, respectively. The formulation is optimal at temperatures above 50°C. However, since proteins may coagulate at temperatures above about 60°C (as illustrated in Table 4), the optimal temperature for prion denaturation is 50-60°C, more specifically 53-57°C.

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PUM

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Abstract

A surface which carries a material which is infected with prions is cleaned with an alkaline cleaning solution to remove as much proteinaceous material as possible from the surface. The cleaning agent is an alkaline cleaning agent which attacks prions remaining on the surface and which attacks prions removed from the surface during the cleaning step. After the cleaning solution is drained, a solution of surfactants, buffers, and heavy metal free corrosion inhibitors is circulated over the surface at 50-60 DEG. The surfactants disperse and unclump the prion-contaminated material. A strong oxidant, preferably peracetic acid, is added to the . solution to bring the peracetic acid concentration to 1,000-2,500 ppm. The peracetic acid or other strong oxidant attacks the prions, particularly the unclumped prion strands, deactivating the prions. After rinsing and drying, the surface may be wrapped in a microbe impermeable barrier and subjected to terminal sterilization, such as steam autoclaving.

Description

technical field [0001] This application relates to the inactivation of prions. The present application also relates to, and is described with particular reference to, the inactivation of prion-infected material derived from medical and surgical instruments. However, the present invention further relates to the decontamination of other surfaces contaminated with prion-infected material, such as drug preparation equipment, food processing equipment, laboratory animal research equipment, including floors, work surfaces, equipment, cages, fermenters, fluid pipes and many more. Background technique [0002] Prions are proteinaceous infectious agents that cause a similar fatal encephalopathy known as transmissible spongiform encephalopathy (TSEs). These diseases include Creutzfeldt-Jakob disease (CJD) in humans, bovine spongiform encephalopathy (BSE) in livestock, also known as "mad cow" disease in humans, scrapie in sheep, and wasting disease in elk and deer. These diseases ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/02A01N25/30A01N37/02A61K31/327A61L2/00A61L2/14A61L2/18A61L2/20C11D1/88C11D3/04C11D3/06C11D3/20C11D3/33C11D3/37C11D3/39C11D11/00C11D17/00
CPCA61L2/14A61L2/0088A61L2/186A61L2/208A61K31/327A61L2/22C11D11/0023A61L2/0082C11D3/3947A61L2202/24
Inventor 杰拉尔德·E·麦克唐奈赫伯特·J·凯泽凯瑟琳·M·安特洛加珍妮弗·A·斯科科斯
Owner AMERICAN STERILIZER CO
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