68results about How to "Good denaturation effect" patented technology

The present invention relates to outer wall paint, and is especially one kind of high performance nanometer outer wall paint and its preparation process. The nanometer outer wall paint has introduced organosilicon and fluoro resin to result in watertight coating with high permeation resistance and breathing function, water-thinned resin, nanometer titania particle, dispersing assistant and other assistants to make the paint possess well dispersed nanometer titania particle and excellent photocatalytic self-cleaning function.

The invention discloses a drug-loaded microsphere and the preparation method, belonging to the technical field of biomedicine, which comprises following steps: adopting water/oil/water multiple emulsion technology and self-assembly technology of protein to fully mix the water soluble medicine and regenerated silk fibroin solution; adding the mixture in oil phase with emulsifier with certain amount during stirring for emulsification; adding organic solvent and stirring, thereby the silk fibroin is denaturalized and Beta structured to form milky crystalline silk fibroin fine particle, and the medicine is embedded; removing supernatant liquid through centrifugal effect; adding organic solvent; further crystallizing the silk fibroin and embedding the medicine; removing solvent and residual oil phase through centrifugal effect and collecting the microsphere. The average particle size of the silk fibroin drug-loaded microsphere is between 5.84 to 86.27Mum, the embedding rate is 42 to 99%, the drug loading dosage is 2.5 to 8.5%. The drug-loaded microsphere has the advantages of applicability to requirements for different purposes and wide application prospect.

The invention belongs to the technical field of metallurgy of iron and steel, and particularly relates to a calcium treatment process of rod wire alloy steel. The invention adopts a converter smelting-LF (ladle furnace) refining-RH refining-square billet continuous casting production technique. The calcium treatment process is implemented in a way that: after the LF refining finishes, feeding an iron calcium wire, silico-calcium wire or solid calcium wire into molten steel at the linear speed of 50-250m/min to control the mass percent of calcium aluminum in the molten steel at 0.08-0.16%; after the soft blow, carrying out RH treatment; after the RH treatment finishes, carrying out calcium carbonate treatment, and feeding the iron calcium wire, silico-calcium wire or solid calcium wire into molten steel at the linear speed of 50-250m/min to control the mass percent of calcium aluminum in the molten steel at 0.04-0.08%; and carrying out soft blow, and casting. When the treatment process is used for producing high-quality steel, the inclusion level can not deteriorate, the castability of the molten steel is good, the number of continuous casting furnaces is increased, the calcium content of the molten steel is low but does not influence the casting performance, and the treatment process plastifies the inclusions and improves the mechanical properties of steel.

The invention discloses a method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F, which comprises the following steps: (1) primarily enriching and purifying the alcohol extract of lucid ganoderma by use of macroporous resin, and separating a ganoderic acid part with purity of 30-60% from the alcohol extract of lucid ganoderma; (2) separating the separated ganoderic acid part through high-speed counter-current chromatography to obtain ganoderic acid with relatively high purity, wherein a solvent system consists of petroleum ether, ethyl acetate, methanol and water at a volume ratio of 5:(3-7):(1-6):(4-9); the upper phase of the solvent is a stationary phase, and the lower phase is a mobile phase; a sample is dissolved with the lower phase solution and fed; the flow velocity of the stationary phase is 10-20mL/min, the flow velocity of the mobile phase is 1.5-3mL/min, and the rotation speed of the host is 600-1,000rpm; the separated object is collected by an automatic fraction collector, and one fraction is collected from every 4-10mL; (3) analyzing and detecting the components of the liquid collected by each test tube by high performance liquid chromatography, collecting, concentrating and drying according to different components, and separating to obtain ganoderic acid C1 and ganoderic acid F with purity up to 95%. The method has the characteristics of high efficiency, high speed, large separation quantity, high recovery rate, solvent saving and the like.

The invention discloses a method for extracting astaxanthin from a fresh shrimp shell. The method comprises the following steps: boiling fresh shrimp shell with boiling water, fishing out, draining, putting into a refrigerator, freezing, taking out, and crushing with a plant crusher; extracting with an organic solvent under an ultrasonic-assisted condition, so as to obtain an extract liquid; carrying out vacuum concentration, putting a concentrated liquid into the refrigerator, and storing; collecting the extracted shrimp shell residue, freezing in the refrigerator, taking out and thawing at a room temperature, and repeating the freezing-thawing process; mixing the repeated frozen and thawed shrimp shell residue with the organic solvent, stirring, extracting, and carrying out vacuum suction filtration, so as to obtain an extract liquid I and shrimp shell filter residues; repeatedly extracting the shrimp shell filter residues, so as to obtain an extract liquid II; collecting the extract liquid I and the extract liquid II by virtue of a rotary evaporator for vacuum concentration; and mixing the obtained concentrated liquid with the concentrated liquid of the extract liquid, and carrying out freeze-drying, so as to obtain an astaxanthin crude extract. The method has the advantages of being simple to operate, low in cost, and convenient to popularize and apply; the extraction process can be carried out at room temperature; and high extraction rate of the astaxanthin is ensured.

The invention provides a method for brewing a soy sauce through puffing soybeans. The method comprises the steps of pretreating raw materials: performing dust removal and impurity removal on soybeans,cleaning the soybeans after dust removal and impurity removal, crushing the cleaned soybeans to obtain bean granules, performing dust removal and impurity removal on bran, and cleaning the bran afterdust removal and impurity removal; puffing the raw materials: puffing the bean granules and the bran; preparing mother culture: weighing the puffed bran, flour and water, uniformly mixing the weighedbran with the weighed flour and the weighed water in the mass ratio of the weighed bran to the weighed flour to the weighed water being 4 to 1 to 4, performing sterilization, performing inoculation with aspergillus oryzae spores, and performing culturing until the mother culture is mature; preparing finished culture: weighing the puffed bean granules, the flour, the puffed bran and distilled water, uniformly mixing the weighed puffed bean granules with the weighed flour, the weighed puffed bran and the weighed distilled water in the mass ratio of the weighed puffed bean granules to the weighed flour, to the weighed puffed bran to the weighed distilled water being 20 to 16 to 1 to 16, performing sterilization, performing scattering, performing inoculation with mature mother culture havingthe same mass, and performing culturing until materials are covered with peak green spores so that finished culture is obtained; and preparing undecanted wine and performing fermentation: uniformly mixing the finished culture with salt water in the volume ratio of the finished culture to the salt water being 1 to 1.7, performing loading into a jar, and performing fermentation. Compared with a traditional technology, the method for brewing a soy sauce through puffing soybeans provided by the invention has the advantages that the time for brewing the soy sauce is shortened, the utilization rateof protein and the yield rate of the soy sauce can be increased, and the soy sauce is outstanding in palatable taste and unique in flavor and has scorch aroma compared with finished products in the prior art.

The invention relates to the technical field of food processing, in particular to an oyster preservation method. The oyster preservation method comprises the following steps: (1) opening shells to take meat: opening shells of oysters to take meat, and cleaning the meat with clear water; (2) rinsing: rinsing the oyster meat obtained in the step (1) in rinsing liquid for 8-12 minutes; (3) ultrasonic treatment: performing ultrasonic treatment on the oyster meat obtained in the step (2); and (4) photodynamic sterilization: soaking the oyster meat obtained in the step (3) in a photosensitizer solution, then immediately taking out the oyster meat, and performing LED light source irradiation to complete the sterilization process. According to the oyster preservation method, the quality of the oyster meat can be effectively improved, so that the eating quality of the sea oyster meat is improved, meanwhile, the oysters can be effectively sterilized in a photodynamic sterilization mode, the shelf life of the oysters is obviously prolonged, and economic benefits are increased.

The invention discloses a complete granulated feed for suckling pigs and a preparation method thereof, which belong to the technical field of animal feed. The complete granulated feed comprises the following raw materials in parts by weight: feed main material 1000, a granulate modifier 40-50, a lactococcus lactis premix 20-30, nutritive substance 25-35, traditional Chinese medicine substance 20-25, western medicine additives 10-15, mineral substance 10-15, milk 55-85, and vegetable oil 10-15, wherein the feed main material comprises corn, soybean cake, wheat bran, whey powder, sugar beet, fish meal, buckwheat, peanut shell, milk powder, egg shell, glucose and salt; the granulate modifier comprises puffed corn powder, starch, puffed soybean meal, soybean lecithin, puffed meat and bone meal, tartaric acid, sodium bicarbonate, sucrose esters, ethanol and non-dairy creamer. The palatability and the utilization rate of the feed are improved, the nutritive components of the feed are enriched and balanced, and the waste of the feed is reduced.

The invention discloses a rapeseed oil squeezing method with a high conversion ratio and belongs to the technical field of vegetable oil squeezing. The method comprises the following specific steps: S1: raw material cleaning; S2: high-temperature frying; S3: grinding blank forming; S4: blank steaming treatment; S5: oil material squeezing; S6: deacidification of fats and oils; and S7: static settlement. According to the method, prior to oil squeezing, oil materials are firstly cleaned and crushed and then are subjected to high-temperature heat treatment, thus, interior of the oil materials canbe subjected to a series of changes such as destruction of oil material cells, prompting of protein denaturation and lowering of viscosity of the fats and oils so as to be applicable to squeezed oil squeezing and oil yield increase, unsaturated fatty acids such as linoleic acid in the fats and oils are not destroyed, nutritional ingredients and natural flavors of crude oil in oil-bearing crops arereserved, and chemical residual is absent.

The invention discloses a DNA extracting method for analyzing the MSAP of epihylla. The method comprises the following steps: taking grafted and rejuvenated young epiphylla leaves as a material, adopting a CTAB (Cetyl trimethyl Ammonium Bromide) method to perform whole genome DNA extraction, removing impurities and preventing browning through improving the concentration of Beta-mercaptoethanol and PVP (Polyvinyl Pyrrolidone), adding chloroform isoamylol, performing secondary extraction to remove proteins effectively so as to obtain high-quality and high-concentration epiphylla genome DNA, analyzing by an MSAP technology, and displaying the methylation polymorphism of the DNA. The extracting cost is low in cost, can obtain the high-concentration genome DNA, and lays the foundation for researching the epigenetics of epiphylla.

The invention relates to the technical field of disinfection wet wipes, and discloses a medical disinfection wet wipe which is prepared by the following steps: reacting cellulose with epoxy chloropropane to obtain epoxidized cellulose, reacting the epoxidized cellulose with 1, 10-decanediol to obtain hydroxylated alkyl modified cellulose, reacting with the hydroxylated alkyl modified cellulose to obtain chloroalkylated cellulose, and further reacting the chloroalkylated cellulose with N,N-dimethyl dodecylamine to obtain obtain quaternary ammonium salt modified alkylated cellulose, dissolving the quaternary ammonium salt modified alkylated cellulose in ethanol to form an antibacterial solution, and infiltrating a non-woven fabric in the antibacterial solution to obtain the medical disinfection wet tissue capable of promoting wound healing. The cellulose can stimulate the proliferation of local tissue cells in the wound, which is conducive to wound healing. A long alkyl chain is introduced into a cellulose matrix, so that the adhesion of cellulose can be improved, the rapid hemostasis effect is achieved, quaternary ammonium salt can promote bacterial cell protein to be denatured and destroy the interiors of cells, and then growth and reproduction of bacteria are inhibited, so that the disinfection and antibacterial performance of the medical disinfection wet tissue is enhanced.

The invention discloses an efficient and safe crop straw feed fermentation inoculant, a solid additive and a preparation method thereof. The preparation method includes: selecting a group of three-class nine strains of fungi, actinomycetes and bacteria which are high in functionality and combining efficiency; subjecting the strains to strain purification and rejuvenation, single-strain enlarged cultivation, mixed liquid fermentation and secondary enlarged cultivation of a concentrated mother liquid inoculant. In the preparation method, the whole preparation process is completed under the conditions of drainage and ventilation of the concentrated mother liquid inoculant in a solid straw feed semi-open fermentor. The straw feed biological solid additive after feed fermentation of various plant straws has capability of efficiently decomposing and softening straw fibers, can improve palatability, animal digestion capability and straw utilization efficiency; a microbial inoculant can degrade pesticide residue in straw and reduce infection of chemically harmful substance and becomes a substitute product of antibiotics, safety and hygiene value of using the straw to produce feed are improved, and the efficient and safe crop straw feed fermentation inoculant becomes an agroecological feed additive.

The invention relates to a roxburgh rose active extract with an auxiliary protection effect on acute alcoholic liver injury. The active extract is prepared by the following steps: performing alcohol reflux extraction on freshly squeezed roxburgh rose pomace or roxburgh rose fruits, filtering, concentrating to remove ethanol, adding water to disperse water insoluble precipitate, filtering, sequentially washing the precipitate with water and petroleum ether or n-hexane, removing water-soluble substances and grease, and drying. The active extract is rich in triterpene and flavone components, has an auxiliary protection effect on acute alcoholic liver injury, can be used for preparing medicines and auxiliary medicines for auxiliary protection of acute alcoholic liver injury or used as a healthy food additive, and has relatively high application value and market prospect. The roxburgh rose extract has important significance in high-value utilization and sustainable development of the rosa roxburghii tratt fruit industry, an intermediate or additive raw material is provided for medicines or health-care foods for auxiliary protection of acute alcoholic liver injury, and the preparation method is simple, easy to implement, low in cost and capable of realizing industrial production.

The invention provides a microsphere for double protection of antibody drug and intravitreal injection, and a preparation method thereof. Particles of a monoclonal antibody drug are prepared by adopting a method of water phase-water phase emulsification, polylactic acid-glycolic acid copolymer and polyketal are utilized as carrier materials, and a sustained release microsphere that wraps and carries the particles of a dextran-monoclonal antibody drug is prepared by employing an emulsion solvent volatilization method of water phase-oil phase-solid phase. Denaturation and aggregation of antibodies are caused by an acidic microenvironment of polylactic acid-glycolic acid in an organic phase/aqueous phase interface and a degradation process, the drug loading capacity of the microsphere is increased and a burst release phenomenon during a releasing process of the microsphere is reduced, and double protection on the monoclonal antibody drug of the microsphere is achieved; the polyketal enables the drug loading capacity of the microsphere to be increased, enables the stimulation of an acidic degradation product of the polylactic acid and glycolic acid on eye environment to be reduce, andenables side effects such as endophthalmitis to be reduced; and the microsphere can release the monoclonal antibody for not less than 28 days in the in vitro environment and for not less than 2 monthsin the eye, and so, the frequency of administration can be reduced, pain of patients and economic burden are reduced, and the compliance of patients is improved.

The invention provides a coix seed and black bean fermented sauce and a preparation method thereof. The preparation method comprises the following steps: respectively roasting coix seeds and black beans, crushing and sieving to obtain coix seed powder and black bean powder; performing puffing treatment on the coix seed powder, the black bean powder and the vital gluten to obtain puffed raw materials; preparing aspergillus oryzae, monascus and corynebacterium glutamicum into a mixed bacterium suspension; moistening and stirring the puffed raw materials and the mixed bacterial suspension with water to obtain a mixture, and subjecting the mixture to ventilation and starter propagation to make a koji; fully mixing the finished koji and saline water and subjecting the mixture to heat preservation fermentation; after the fermentation for 18-22 days, adding a mixed seed solution of saccharomyces rouxii and torulopsis torulosa into the raw materials, continuing the fermentation; after fermentation for 60-90 days, completing preparation of the fermented sauce. The coix seed and black bean fermented sauce can effectively solve the problem that the application range of the existing coix seeds is limited. The invention provides the fermented sauce with advantages in nutrition and functional value, which meets the requirements of modern consumers for health, nutrition and low salt, and widens the application range of the coix seeds.

The invention discloses a step-by-step deoxidation operation method suitable for low-carbon steel. The step-by-step deoxidation operation method comprises the following specific steps that a deoxidation route of low-carbon steel production carries out deoxidation by adopting a step-by-step operation method of a converter and a steel ladle, as follows: 1, carrying out precipitation deoxidation on molten steel in the converter by adopting an aluminum deoxidizer in the earlier stage of tapping; and 2, tapping the deoxidized molten steel in the converter to the steel ladle, and alloying by adopting secondary deoxidation, wherein the deoxidation sequence is from weak to strong by adopting a mode of calcium carbide, low-carbon ferromanganese, aluminum ferromanganese and silicon-calcium alloy.

The invention provides a Hirsutella sinensis fermented powder extract. The heavy metal content of the extract is lower than that in the international food hygiene standard, the extract does not have pesticide residue, can be completely dissolved in water or ethanol and does not contain culture medium residue, and the product is difficultly oxidized, difficultly generates smell of mutton, and has long retention period. The extract has obvious activity of resisting renal failure, 0.2 to 1.0g/Kg extract can obviously reduce serum creatinine (SCr) and blood urea nitrogen (BUN) content of a rat with renal failure, improves nephridial tissue degeneration caused by gentamicin, inverses renal tubular epithelial cell proliferation inhibition caused by aristolochic acid, reduces mRNA expression of renal failure key factors (TGF beta 1, TIMP-1, PAI-1 and ET) and has other effects; and compared with that of Hirsutella sinensis fermented powder, the effect of the Hirsutella sinensis fermented powder extract is improved by 2 to 10 times, the dosage can be greatly reduced, and the Hirsutella sinensis fermented powder extract is conveniently used by the patient and can be applied to preventing and treating renal failure disease. The Hirsutella sinensis fermented powder extract can serve as an active ingredient to be mixed with pharmaceutic excipients so as to prepare granules, capsules, tablets, buccal tablets, oral solution, tinctures and other formulations.

The invention relates to a preparation process for a fattening cream river crab compound feed and belongs to the technical field of agricultural breeding. The compound feed is prepared from the following materials: high protein flour, peeled expanded bean pulp, fermented bean pulp, fish meal, chicken powder, shrimp powder, sleeve-fish paste, phospholipid oil, soya-bean oil, fish oil, spirulina, choline chloride, zeolite powder, monocalcium phosphate, table salt, yeast hydrolysate, antibacterial peptides, chitosan compound vitamins, compound organic microelements and astaxanthin. By roughly crushing the materials first and then carrying out ultraomicro crushing on the materials, the materials are fine enough. The materials are mixed in grade and can be fully mixed, so that the fusing degreeof the powder and the oil is improved, and follow-up tempering and expanding operations are facilitated. The longer the tempering time is, the longer the contact and mix time of vapor and the powderyfeed is, and the more the added vapor amount is, the more favorable the curing of the river crab feed is. The tempered materials are extruded and expanded, so that starch is fully cured, the proteinsare deteriorated, the antinutritional factors in different feed raw materials are deactivated, and insects are killed and bacteria are sterilized, and therefore, the digestive absorption utilizationof the feed by the river crabs is improved, the intestinal tract irritation is reduced, diseases are reduced, and the water quality is improved.

The invention relates to a soybean milk preparation method capable of effectively controlling false boiling of a soybean milk machine. The soybean milk machine comprises a cup body, a smashing device,a heating device and an overflow prevention electrode, the smashing device is arranged in the cup body, the heating device heats the cup body, the method comprises (a) smashing stages: including a primary smashing stage and a secondary fine smashing stage, the primary smashing stage is the stage where water and materials are subjected to primary smashing, the secondary fine smashing stage is thestage where a milk liquid obtained after primary smashing is subjected to fine smashing again, a false boiling identification step is arranged between the primary smashing stage and the secondary finesmashing stage, and the false boiling identification step is the stage where the milk liquid obtained after primary smashing is heated to touch the overflow prevention electrode, and then the secondary fine smashing stage is started again. Compared with the prior art, the nutrients can be well released and the flavor can be formed, false boiling can be effectively controlled, and the smashing fineness is high.

The invention relates to an adding treatment agent for preparing PMHSS, in particular to a refining agent for secondary refining refining of the PMHSS. The refining agent comprises a primary refining agent for performing desulfuration and deoxidation during primary refining and a secondary refining agent for performing secondary refining after the primary refining. At the same time, the invention further relates to a secondary refining method of the PMHSS applying the refining agent and a preparation method of the PMHSS applying the secondary refining method. Two times of waste washing are adopted, that is, the primary refining agent is adopted for the first waste washing, so that desulfuration and deoxidation on molten steel are realized, then the secondary refining agent is added to remove impurities, and the purity of the molten steel is effectively improved.

The invention provides a kit for extracting plasma free DNA by a paramagnetic particle method and a use method of the kit. The kit comprises a lysis solution, protease K, a binding solution, a magnetic particle solution, a first cleaning solution, a second cleaning solution and an eluent, wherein the lysis solution and the binding solution are concentrated to prepare lysate freeze-dried powder and binding solution freeze-dried powder respectively. According to the kit, through a specific reagent combination, repeated centrifugation is not needed in the use process, the total amount of plasma free DNA is guaranteed, cross contamination possibly caused in the centrifugation process is avoided, and the DNA purity is improved.