13results about How to "High drug loading efficiency" patented technology

The invention belongs to the technical field of medical materials, and particularly relates to a degradable wound dressing and a preparation method thereof. Compared with the prior art, the degradable wound dressing prepared by the preparation method disclosed by the invention is a completely biodegradable Janus composite film, and a hydrophobic inner layer and a hydrophilic outer layer are obtained by selecting different materials; the material PGA with excellent biological performance is utilized, the problem that electrostatic spinning cannot be carried out through pure PGA is solved, PGA and gelatin are creatively mixed, the advantages of PGA and gelatin are combined, and the PGA / Gel electrostatic spinning film which is high in toughness, high in tensile strength, good in biocompatibility and high in medicine carrying efficiency is obtained; meanwhile, the functions of resisting bacteria, resisting inflammation, discharging seepage and promoting angiogenesis are achieved, and the wound healing rate is effectively guaranteed.

The invention belongs to the technical field of medicines, and discloses a cationic lipid C6-A1, a nano-liposome particle composed of the same and a preparation method of the nano-liposome particle, and the stability is improved: the stability of the nano-liposome can be remarkably improved through the structural design and PEG modification of the C6-A1 lipid, and in-vivo degradation is reduced. And the optimized preparation method and introduction of cationic lipid can improve the encapsulation efficiency of drugs and genes. And the PEG modified lipid can reduce the recognition of an immune system, prolong the circulation time of the liposome in blood and improve the targeted transmission capability. The cation characteristic of the C6-A1 is beneficial to the combination and endocytosis of the gene and a cell membrane, and the gene transfer efficiency is improved.

The invention relates to the technical field of biomedical materials, in particular to a hydrogel microneedle for skin improvement and a preparation method thereof.The hydrogel microneedle for skin improvement comprises a microneedle matrix and fisetin, the microneedle matrix is a hydrogel matrix, and the fisetin is embedded in the microneedle matrix. According to the hydrogel microneedle for skin improvement, the hydrogel microneedle has good mechanical performance, biocompatibility and degradability, the effect of slowly releasing medicine can be achieved, the hydrogel matrix wraps fisetin, the targeting performance of anti-aging active ingredients can be effectively enhanced, and the anti-aging effect of the skin is improved. The anti-aging agent is slowly released in a corium layer to play an anti-aging role, and has a wide application prospect in medical beauty and skin care products.

The invention discloses a preparation method of drug delivery nanoparticles based on hydrogel microneedles, and relates to the technical field of biological medicine, the technical key points are as follows: step S1, preparing a white suspension solution; step S2, preparing a white powdery porous hollow nano microsphere A; step S3, preparing A nano microsphere powder loaded with TP; step S4, preparing a CaCO3 (at) Lip (at) DEC205 nano microsphere preparation; and S5, preparing the PVP microneedle: dropwise adding a proper amount of polyvinylpyrrolidone solution into the mold, keeping the weights of the two six-hole culture plates equal, centrifuging, putting the obtained culture plates into a drying oven, evaporating the solution to dryness, and taking down the prepared microneedle from the mold. The liposome is added, so that the TP drug loading efficiency is higher, and the toxic and side effects of the whole body are reduced.

The invention discloses a nano preparation for regulating an idiopathic pulmonary fibrosis mechanical microenvironment. According to the nano preparation, a zeolite imidazole framework material-8 is used as a carrier, the outer layer of the nano preparation is coated with a polydopamine coating, RGD targeting peptide is combined, and two drugs are synergistically loaded, so that dual regulation and control on the mechanical microenvironment of the lung are realized. The functionalized metal organic framework is used as a drug delivery carrier, and zinc ions and drugs can be responsively released in an acid environment. On one hand, the mechanical tension of the lung is reduced by repairing the damaged type II alveolar epithelial cells and promoting the differentiation of the damaged type II alveolar epithelial cells; on the other hand, lung mechanical signal transmission is inhibited, the contractility of myofibroblasts is reduced, and excessive deposition of extracellular matrixes is reduced. Finally, the nano preparation plays a dual role, the steady state of a lung mechanical microenvironment is recovered, and a brand new strategy and method are provided for treatment of idiopathic pulmonary fibrosis.

The invention discloses a new application of a proton pump inhibitor. The proton pump inhibitor realizes treatment on related diseases and enhances the treatment effect by promoting endocytosis of extracellular vesicles. The proton pump inhibitor is used for enhancing anti-tumor immunity; the proton pump inhibitor is used for relieving pd-1 drug resistance in tumor treatment; the proton pump inhibitor is used for enhancing the drug delivery function of the extracellular vesicles; the proton pump inhibitor is used for removing extracellular vesicles. The invention finds that the proton pump inhibitor can enhance anti-tumor immunity, reduce drug resistance, enhance the delivery effect of extracellular vesicles on therapeutic drugs and reduce side effects of the drugs. The invention provides a pharmaceutical composition containing the proton pump inhibitor, and the pharmaceutical composition has a wide application prospect.

The present invention relates to a stimulus-responsive dual-drug co-delivery micelle and a preparation method and application thereof, belonging to the field of polymer chemistry and pharmaceutical preparation. The present invention uses doxorubicin (DOX) directly as a hydrophobic block, and bonds with the hydrophilic block mPEG through a disulfide bond that can respond to a high concentration of glutathione in the tumor microenvironment to form an mPEG-SS-DOX micelle material, and uses molecular dynamics simulation screening to obtain the Flory-Huggins interaction parameter between curcumin (Cur) and the hydrophobic end of mPEG-SS-DOX to be the smallest. The self-assembly process of drug-loaded micelles and the effect of drug loading on the morphology and structure of micelles are studied by dissipative particle dynamics simulation. The mPEG-SS-DOX / Cur micelles with redox responsiveness are successfully prepared, and the micelle particle size is small, the surface is negatively charged, and the drug loading and encapsulation efficiency are good.

The present application relates to the technical field of nanomedicine, in particular to a β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid nanoparticle carrying ginsenoside CK, a preparation method and application. A first esterification reaction is performed between a β-cyclodextrin solution and a carboxyl-activated carboxymethyl chitosan solution to obtain a β-cyclodextrin-carboxymethyl chitosan polymer; a second esterification or amidation reaction is performed by adding carboxyl-activated deoxycholic acid to the β-cyclodextrin-carboxymethyl chitosan polymer to obtain a β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid polymer; an ethanol solution of ginsenoside CK is mixed with a water solution of the β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid polymer to obtain a whole particle, and a freeze-drying protective agent is added for freeze-drying to obtain the β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid nanoparticle carrying ginsenoside CK. The present application focuses on the design of an intelligent carrier, realizes the synergistic optimization of high drug loading and intelligent delivery, and overcomes the defects of the prior art.

The invention discloses an application of a Lamp2b-IMTP fusion exosome in treatment of coronary heart disease. The specific application method comprises the following steps: preparing an exosome matrix, carrying out optimization treatment on the exosome matrix, and finally, specifically combining the optimized exosome matrix with myocardial cells through IMTP on the surface of the Lamp2b-IMTP fusion exosome in an intravenous injection administration mode. Therapeutic substances are accurately delivered to damaged myocardial tissues, which is the core advantage of the fused exosome and is also the basis of subsequent treatment effects: IMTP (myocardial targeting peptide) is modified on the surface of the exosome, and the IMTP can be accurately combined with a specific receptor on the surface of a damaged myocardial cell.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to a hyaluronic acid-modified co-supported nanoparticle, its preparation method, and its application. The nanoparticle has a core-shell structure, with the core being a coordination complex of zinc-doped Prussian blue and sinomenine hydrochloride, and the outer shell being a bovine serum albumin-hyaluronic acid conjugate. This invention improves the therapeutic effect on knee osteoarthritis.

The invention provides a nasopharynx cancer targeting nano train drug delivery system, and belongs to the field of nano materials and biomedical engineering, the targeting specificity of a nucleic acid aptamer S3 is utilized, nano train loading chemotherapeutic drug adriamycin is coupled, and nano train drug-loading nanoparticles modified by the nucleic acid aptamer S3 are prepared and used for targeted killing of NPC cells and tissues. The aptamer-modified nano train can reduce toxic and side effects on normal tissues and cells while remarkably improving the loading capacity of the medicine and improving the targeting property on nasopharyngeal carcinoma; besides, the nucleic acid aptamer S3, the NK cells and the combined S3-NK cells are prepared, the targeting characteristic of the nucleic acid aptamer and the immunocompetence of the NK cells are fully utilized through the combined strategy, a treatment platform with a synergistic effect is formed, the targeting delivery and treatment effect on nasopharyngeal carcinoma cells is remarkably improved, and wide clinical application prospects are shown.

The invention belongs to the technical field of stimuli-responsive pharmaceutical preparations, and particularly relates to a stimuli-responsive bionic drug-loaded nanoparticle based on polypyrrole and a preparation method thereof. The preparation method comprises the following steps: reacting pyrrole with an oxidizing agent to obtain a polypyrrole skeleton, coupling the polypyrrole skeleton with halogenated methylphenylboronic acid, and coupling a phenylboronic acid group with a drug containing a vicinal diol or propylene glycol structure through a dehydration reaction, then, the polypyrrole skeleton can adsorb charged drugs, and finally, the biomimetic drug-loaded nanoparticles are obtained by wrapping cell membranes. The bionic nanoparticles can recognize related receptors expressed by a blood brain barrier, reach the blood brain barrier in a targeting manner, are internalized into the brain, are enriched to a brain lesion, and selectively release related drugs under the stimulation of high-concentration active oxygen, abnormal bioelectricity and acidic pH of lysosome in neuronal cells of the lesion; the curative effects of protecting neurons, inhibiting reactive glial hyperplasia and repairing damaged blood-brain barriers are achieved; meanwhile, phenylboronic acid groups can remove active oxygen of focuses, oxidative stress is relieved synergistically, and inflammatory response is relieved.

The present invention belongs to the field of biopharmaceuticals, and is particularly related to the application of ultrasonic microfluidics in exosome (exo) drug encapsulation. The drug loading efficiency of exo-siRNA prepared by ultrasonic microfluidics is about 1-2 times that of electroporation, common ultrasonic method and incubation method; Ultrasonic microfluidics uses cavitation to quickly mix the entire mixed system uniformly, so that siRNA quickly enters exosomes, with high drug loading efficiency, and compared with common ultrasonic methods, ultrasonic microfluidics can complete the encapsulation of siRNA in a shorter time through a single low-power ultrasound, and has less effect on the membrane structure of exosomes and the structure of siRNA; 293F-exo prepared by ultrasonic microfluidics 293F-exo-siRNA can be quickly taken up by cells, therefore, ultrasonic microfluidics provides a new and effective encapsulation method for exosomes as drug carriers.