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Preparation method of amino and polypeptide modified AIE polymer nanoparticles

The invention relates to a preparation method of amino and polypeptide modified AIE polymer nanoparticles, the preparation method comprises the following steps of: 1) dissolving an emulsifier and an amino functional monomer in water to obtain an aqueous phase solution; 2) dissolving AIE molecules and an Austrian ripening effect inhibitor in a hydrophobic monomer to obtain an oil phase solution; 3)adding the aqueous phase solution into the oil phase solution, stirring and pre-emulsifying to obtain crude emulsion, and performing ultrasonic treatment to obtain monomer miniemulsion; introducing nitrogen to remove oxygen, adding a water-soluble initiator to react to prepare amino modified AIE polymer nanoparticle emulsion; 4) dissolving omega-maleimide alkyl acid and a carbodiimide condensingagent in an acidic pH buffer solution for activation to obtain an activated intermediate solution; 5) adding the activated intermediate solution into the emulsion prepared in the step 3) to react to prepare maleimide modified AIE polymer nanoparticle emulsion; and 6) adding an aqueous solution of a polypeptide containing cysteine sequence units at the end to the emulsion prepared in the step 5) for reaction to obtain the amino and polypeptide modified AIE polymer nanoparticles.
Owner:ZHEJIANG SCI-TECH UNIV

Method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles

A method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles comprises the following steps: (1) dissolving an emulsifier and an amino functional monomer in water to obtain an aqueous solution; (2) dissolving an AIE molecule, an Ostwald ripening effect inhibitor and an oil-soluble initiator in a hydrophobic monomer to obtain an oil phase solution; (3) adding the aqueous solution to the oil phase solution, stirring and pre-emulsifying the obtained mixture to obtain a crude emulsion, carrying out ultrasonic treatment to obtain a fine monomer emulsion, introducing nitrogento remove oxygen, and carrying out a reaction to produce an amino-modified AIE polymer nanoparticle emulsion; (4) dissolving omega-maleimido alkyl acid and a carbodiimide condensing agent in an acidicpH buffer solution, and performing activation to obtain an activated intermediate solution; (5) adding the activated intermediate solution to the emulsion prepared in step (3), and carrying out a reaction to obtain a maleimide-modified AIE polymer nanoparticle emulsion; and (6) adding an aqueous solution of a polypeptide containing a cysteine sequence unit at the end to the emulsion prepared in step (5), and carrying out a reaction to produce the amino acid-and-polypeptide-modified AIE polymer nanoparticles.
Owner:ZHEJIANG SCI-TECH UNIV

High-yield disease-resistant planting method of trichosanthes kirilowii maxim

The invention discloses a high-yield disease-resistant planting method of trichosanthes kirilowii maxim. The method includes the steps of (1) field preparation, wherein the ground surface of a planting field is leveled, deep and wide gullies are dug at intervals, and after the soil of the planting field is defrosted in the early Spring, base fertilizer and attapulgite are applied into the soil; (2) sowing; (3) planting, wherein planting holes are dug, humic acid fertilizer and humic organic fertilizer are evenly blended and used for filling the planting holes, vegetable seedlings are buried into the planting holes after punning, planting soil covers the seedlings, and planting water is sprayed after punning; (4) management of fertilizer and water, wherein during the period from the later August to the middle September, organic slow release fertilizer is applied to the planting field; before accelerating germination, accelerating germination fertilizer is applied for dressing; 10 days before blossoming, compound fertilizer of nitrogen, phosphorus and potassium is applied for dressing, and boric fertilizer is applied to leaf surfaces in the process of applying the compound fertilizer of nitrogen, phosphorus and potassium. The high-yield disease-resistant planting method of trichosanthes kirilowii maxim can effectively improve disease resistance of trichosanthes kirilowii maxim, further increase yield of trichosanthes kirilowii maxim and further improve quality of trichosanthes kirilowii maxim.
Owner:安徽太白庄园生态农业有限公司

VNSAK polypeptide and application thereof

ActiveCN104341530AOvercome weak immunogenicityOvercoming the drawbacks of immune toleranceMammal material medical ingredientsBlood/immune system cellsEpitopeSART3
The invention discloses a VNSAK polypeptide and an application thereof. The VNSAK polypeptide sequentially comprises an epitope zone and an endoplasmic reticulum retention base sequence zone from an end N to an end C; the epitope zone comprises an SART3-109 epi-position, an HNRPL-501 epi-position and an NY-ESO-1 epi-position; the SART3-109 epi-position is indicated as amino acid residues from the first position to the tenth position of the N end of a sequence 1 of the sequence table, the HNRPL-501 epi-position is indicated as the amino acid residues from the eleventh position to the twentieth position of the N end of the sequence 1 of the sequence table, the NY-ESO-1 epi-position is indicated as the amino acid residues of the twenty-first position and the twenty-ninth position of the N end of the sequence 1 of the sequence table, and the endoplasmic reticulum retention base sequence zone is indicated as the amino acid residues of the thirty-third position to the thirty-sixth position of the N end of the sequence 1 of the sequence table. The invention also protects the application of the VNSAK polypeptide in preparing tumor treating medicines. The VNSAK polypeptide and the application thereof have an important significance on treating the tumor.
Owner:CHONGQING QINLIAN BIOMEDICAL TECH

Folate receptor-targeted antihypertensive peptide composition and preparation method thereof

The invention discloses a folate receptor-targeted antihypertensive peptide composition and a preparation method thereof, and relates to antihypertensive peptide. The antihypertensive peptide composition is prepared from 0.5-10 parts of antihypertensive peptide VP5, 0.1-5 parts of folate-modified lipid, 1-30 parts of phospholipid, 0.25-10 parts of cholesterol and 10-100 parts of a polyester material. The preparation method comprises the steps that 1, polyester nanoparticles loaded with the VP5 are prepared; 2, the folate-modified lipid, phospholipid and cholesterol are dissolved in an organic solvent, pressure reduction is conducted, rotary evaporation is conducted to remove the organic solvent, a thin film is obtained, the polyester nanoparticles loaded with the VP5 are added, hydration is conducted to remove the thin film, homogenizing or extruding is conducted after ultrasonic treatment is conducted, and the antihypertensive peptide composition is prepared; or the folate-modified lipid, phospholipid and cholesterol are dissolved in the organic solvent, pressure reduction is conducted, rotary evaporation is conducted to remove the organic solvent, a homogeneous thin film is prepared, then a normal saline solution or a glucose solution or a phosphate buffer solution is added, hydration is conducted to remove the thin film, the polyester nanoparticles loaded with the VP5 are added, homogenizing or extruding is conducted after ultrasonic treatment is conducted, and the antihypertensive peptide composition is prepared.
Owner:SHENZHEN POLYTECHNIC

Copolymerization micelle drug-loading nanoparticle and application thereof

The invention discloses a copolymerization micelle drug-loading nanoparticle. The copolymerization micelle drug-loading nanoparticle is a block copolymer comprising a discharged hydrophilic polymer chain block, a discharged hydrophobic polymer chain block and a cationic polymer chain block. The discharged hydrophilic polymer chain block and the cationic polymer chain block are positioned at two ends of the discharged hydrophobic polymer chain block through chemical bonds. The cationic polymer chain block is grafted with a lipid ligand. The nano drug-loading particle as a triblock copolymer self-assembled micelle system is capable of realizing co-transmission of siRNA and a chemotherapy drug. A triblock copolymer is amphipathic, and can be self-assembled into the micelle nanoparticle. Preparation cost is low and preparation is convenient. A hydrophilic part is capable of reinforcing circulation time. The discharged hydrophobic polymer chain block is used for wrapping adriamycin so as toform a hydrophobic kernel. The cationic polymer chain block carries positive charge, so that the negative charge siRNA can be carried. Cholesterol is connected to the cationic polymer chain block, sothat efficiency of endocytosis is improved.
Owner:WENZHOU MEDICAL UNIV

Disease-resistant planting method of nursery stocks

The invention discloses a disease-resistant planting method of nursery stocks. The method comprises the following steps of 1, soil preparation, wherein sandy soil which is deep in soil layer, loose and fertile, and good in drainage is selected, base fertilizer is spread, the soil is covered with thin film for 5-7 days, the thin film is uncovered, the soil is subjected to deep ploughing and fine tillage, bedding is conducted, and the bed surface is flattened for use; 2, planting, wherein planting pits are dug, humate fertilizer and decomposed organic fertilizer are uniformly mixed to fill the planting pits with the mixture, after tamping, the nursery stocks are buried into the planting pits, then the planting pits are covered with planting soil, and after tamping, the pits are watered withenough field planting water; 3, fertilization, wherein ditching is conducted, organic slow release fertilizer is applied, after topdressing, watering is conducted, maize straw powder is utilized to cover the pits, the moisture is preserved, foliage dressing is conducted from June to July, and spraying is conducted at dawn or dusk when dew exists. The disease-resistant planting method of the nursery stocks can effectively improve the disease-resistant capability of the nursery stocks, and can improve the planting efficiency and planting quality of the nursery stocks.
Owner:芜湖浪尖山生态林业有限公司
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