Preparation method for tumor antigen targeting DC cells
A tumor antigen, targeted technology, applied in the field of cell culture, to achieve the effect of strong tumor-specific immune response, wide variety, and efficient uptake
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Embodiment 1
[0039] Step 1: Prepare Tumor Tissue Lysate
[0040] 1.1 Take fresh in situ resected gastric cancer tissue positive for CEA.
[0041] 1.2 Remove the normal tissue above the surgically resected tumor tissue, rinse with normal saline until there is no blood and no fat residue.
[0042] 1.3 Soak with 0.1% chlorhexidine (chlorhexidine) for 30 minutes, rinse with sterile saline 3 to 4 times.
[0043] 1.4 Shred the tumor tissue with sterile surgical scissors, add an appropriate amount of saline for grinding, and collect the single cell suspension after filtering through a 200-mesh mesh.
[0044] 1.5 Put the single-cell suspension into a centrifuge tube, freeze at -86°C, thaw at room temperature, repeat freezing and thawing 10 times, and observe under a microscope until no cells survive.
[0045] 1.6 Centrifuge the single cell lysate at 3000rpm for 30min. Aseptic operation The supernatant was filtered and sampled with a 0.22 μm pore size sterile filter and stored at 4°C for quality...
Embodiment 2、3
[0056] The operation process of steps 1-5 is the same as that of Example 1, except that the concentration and time of adding CEA antibody, tumor tissue lysate in step 3 are different:
Embodiment 2
[0057] Example 2: DC cells were cultured until the nth day (n=3), the concentration of anti-CEA antibody was 20 ng / ml, and the concentration of tumor tissue lysate was 100 ug / ml.
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