12results about How to "Increase load factor" patented technology

The invention provides a resveratrol-loaded exosome and a preparation method thereof, and belongs to the technical field of biological medicine manufacturing. The preparation method of the resveratrol-loaded exosome comprises the following steps: performing 3D culture on human umbilical cord or hair follicle-derived mesenchymal stem cells under a low-oxygen condition, collecting supernate, and separating by a differential ultracentrifugation method to obtain the exosome of the mesenchymal stem cells; the method comprises the following steps: mixing a resveratrol solution containing a surfactant with the MSCs-Exos, and carrying out co-incubation and ultrafiltration to obtain the resveratrol loaded MSCs-Exos. According to the invention, the surfactant is added, so that not only are the entrapment rate and the drug loading rate of the MSCs-Exos loaded resveratrol greatly improved, but also the activity of the MSCs-Exos is maintained, the common effect of the MSCs-Exos and the resveratrol is fully exerted, and a synergistic effect is achieved. The resveratrol loaded MSCs-Exos disclosed by the invention has an effect of treating diabetes mellitus.

The invention discloses a preparation method of stability-enhanced alginic acid-keratin-based double-coated nanoparticles. The preparation method comprises the following steps: carrying out controllable enzymolysis, enzyme deactivation, centrifugation and freeze-drying on keratin to obtain keratin nanoparticles formed by self-assembly; dispersing the keratin nanoparticles in an acidic buffer solution to carry out structure development, adding bioactive molecules, and adjusting the pH to be neutral to recombine and close the keratin nanoparticle structure to obtain a keratin nanoparticle solution loaded with the bioactive molecules; the preparation method comprises the following steps: taking keratin as a template, dispersing the keratin into a sodium alginate solution, dropwise adding into a calcium chloride solution, cross-linking to form a calcium alginate shell, dialyzing or centrifuging to remove free bioactive molecules, and freeze-drying to obtain the bioactive molecule loaded alginic acid-keratin-based double-coated nanoparticles. The invention also discloses double-coated nanoparticles and application thereof, through controllable enzymolysis and pH regulation and control, the self-assembly and bioactive molecule encapsulation of the keratin nanoparticles are improved, and the double-coated nanoparticles with regular shape, good dispersibility, good redissolvability and good environmental stability are formed.

This invention discloses a MaaS dynamic bus route and station planning method and a trip optimization method based on spatiotemporal clustering. The MaaS algorithm based on spatiotemporal clustering, within a defined boundary area of ​​no more than 25 square kilometers, determines bus routes and stops based on existing public transport demand where both origin and destination are within this area, according to established spatiotemporal clustering rules. It then returns the determined bus routes, stops, and estimated arrival times to passengers using the planned bus routes and stops. The application of this invention maximizes the convenience of public transport, reduces unnecessary waiting time and connection distances, increases bus occupancy rates, reduces the probability of empty bus runs, and minimizes stops at unnecessary stops, thereby improving operational efficiency.

The invention discloses a barrel structure for a steel ball coal mill, which comprises a barrel with two closed ends, a transmission shaft and an annular bin, and the transmission shaft is coaxially positioned in an inner cavity of the barrel, penetrates through the two ends and is fixedly connected with the barrel; the annular outer wall of the annular bin is connected to the inner wall of the middle of the barrel in a bolted mode so that an inner cavity of the barrel can be divided into a first bin chamber and a second bin chamber which are equal in volume. The annular inner wall of the annular bin is fixedly arranged on the transmission shaft in a sleeving mode, and a notch is formed in the annular inner wall, opposite to the transmission shaft, of the annular bin so as to communicate the first bin with the second bin to balance the material level. The annular bin is installed in the middle of the barrel, on one hand, the annular bin can occupy the space of the barrel, then the effective volume in the barrel is reduced, and the steel ball loading coefficient is increased by reducing the effective volume of the barrel; on the other hand, the inner cavity of the barrel can be divided into two chambers with the same volume through the annular bin, the balance of the raw coal amount and the steel ball amount in the two chambers is kept through the notch, and it is guaranteed that the steel balls are separated in the two chambers.

The invention belongs to the field of food, and provides a preparation method of mixed fruit vesicles, which comprises the following steps: respectively extracting phenolic compounds in grape pomace, fig pomace and hawthorn pomace to obtain a grape pomace extract, a fig pomace extract and a hawthorn pomace extract; taking at least one of the grape pomace extract, the fig pomace extract and the hawthorn pomace extract to obtain a mixed pomace extract; uniformly mixing the mixed pomace extract with soybean lecithin and water, adding gelatin and / or resistant dextrin, uniformly mixing, and performing ultrasonic treatment to obtain the vesicles. According to the invention, the grape extract, the figs, the hawthorn fruits and the nano-vesicles are organically combined for the first time. The preparation can be easily added into food and beverages, the value chain of fruits such as grapes can be enriched, and the environment and human health can be improved. The invention demonstrates that the preparation, as a novel delivery system, plays a positive role in protecting intestinal tracts and strengthening intestinal microbiomasses.

The invention relates to a mitochondrial delivery system and a preparation method and application thereof. According to the mitochondrial delivery system, safe and efficient cell entry of active mitochondria can be achieved, the mitochondrial delivery system has appropriate particle size and surface potential, toxicity to cells is avoided while the entrapment rate of the mitochondria is effectively improved and the electrochemical stability and biological activity of a mitochondrial membrane are guaranteed, and the mitochondrial cell entry efficiency is remarkably improved. The mitochondrial delivery system not only can transplant active mitochondria into common cells to repair cell damage, but also can transplant the active mitochondria into egg cells to play a role across a zona pellucida and a plasma membrane barrier. The mitochondrial delivery system can be used for treating mitochondrial diseases related to mitochondrial dysfunction, especially infertility caused by mitochondrial dysfunction of egg cells.

The invention discloses an annular direct-current busbar power system used in a ship dynamic positioning mode and a control method of the annular direct-current busbar power system. According to the annular direct-current busbar power system, due to the annular direct-current busbar networking mode, more flexible and rapid protection performance is achieved, that is, before a ship power grid is affected by faults, the frequency converter can rapidly detect abnormal conditions and actively complete isolation of fault points, so that the whole power grid and normal branches are not affected, and after the faults are eliminated, the frequency converter can rapidly detect the abnormal conditions and actively complete isolation of the fault points. And normal connection is quickly recovered. The variable-speed generator set is adopted in the system, the speed regulation range of the variable-speed generator set is expanded, the variable-speed generator set can automatically regulate the running speed according to loads, and load oil consumption can be always kept within an economic range, so that oil is saved, emission is reduced, the energy efficiency utilization rate of the generator set is remarkably improved, and the flexibility of the system is improved. The method is more suitable for ships with ship dynamic positioning working condition requirements.

The invention relates to a preparation method of a metal ion complexation mRNA delivery system and a product and application thereof, and the preparation method comprises the following steps: (1) mixing mRNA and metal ions in a solvent at 10-35 DEG C for 10-60 min to obtain nanoparticles; and (2) mixing the nanoparticles with a phospholipid membrane at 10-35 DEG C for 2-60 minutes, and wrapping the nanoparticles with the phospholipid membrane to obtain the metal ion complexation mRNA delivery system. Metal ions and mRNA are mixed and complexed to form complex nanoparticles, and mRNA loading capacity can be improved. The surfaces of the nanoparticles are coated with phospholipid membranes, so that the biocompatibility of the delivery system is improved, the in-vivo circulation time of the delivery system is prolonged, and meanwhile, fusion between the delivery system and a target cell membrane can be promoted. Besides, the mixing temperature of mRNA and metal ions is controlled to be 10-35 DEG C, the mixing time is controlled to be 10-60 min, the reaction condition is mild and rapid, the prepared nanoparticles are uniform in particle size, and safer and more effective mRNA delivery can be achieved.

The hybrid power system of the four-wheel-drive vehicle comprises an engine, a first motor, a second motor, a front axle transmission part and a rear axle transmission part, the engine is provided with a first input shaft, and power output is connected to the first input shaft; the front axle transmission part comprises a first clutch, a first speed reducer, a hydraulic torque converter, a planetary gear mechanism, a second speed reducer, a third clutch and a differential mechanism; according to the power system of the hybrid power vehicle, only two motors and one engine are used for achieving all functions needed by the four-wheel-drive hybrid power vehicle, and a power assembly is more simplified.

The invention discloses a remote control compensation device capable of bidirectional reactive power compensation and harmonic treatment, and belongs to the technical field of electric energy quality treatment. The problems that an existing device can only provide fixed reactive power compensation and cannot provide needed reactive power compensation according to reactive power changes are solved; and only capacitive compensation is mainly used, the requirement of compensating inductive reactive power cannot be met, and meanwhile, remote control cannot be realized. The remote control device capable of bidirectional reactive compensation and harmonic suppression is applied to the power distribution network, so that the power transmission capacity of the power distribution network and the power supply capacity of a rural power grid distribution transformer can be effectively improved, and the requirements of energy conservation and consumption reduction of the low-voltage power distribution network are met. Meanwhile, the voltage level of the power distribution area can be effectively adjusted, reactive power in the system can be compensated in real time, normal voltage of users at the tail end of the area during the load peak period is guaranteed, three-phase load balance of each node of the area is achieved, and the power supply quality of the area is remarkably improved.

The invention relates to a transmission mechanism and a double-motor electric drive axle assembly. The transmission mechanism comprises a first input shaft and a second input shaft, a sun gear of the first planetary reducer is connected with a first input shaft and a second input shaft, a planet carrier serves as an output end, and the second input shaft is connected with the sun gear through a first one-way transmission part and connected with the planet carrier through a one-way transmission structure. The second input shaft rotates in the first rotating direction, transmits power to the sun gear through the first one-way transmission piece and disconnects power transmission with the one-way transmission structure, and the second input shaft rotates reversely, transmits power to the planet carrier through the one-way transmission structure and disconnects power transmission with the sun gear. When the second input shaft rotates in the first rotation direction or rotates in the reverse direction, the rotation directions of the planet carriers are the same. The power requirements of high-speed driving and climbing working conditions can be met, the power of a single motor is low, the highest rotating speed of the motor is low, the falling point of an efficient area tends to be consistent with the working condition point of the whole vehicle, and the utilization rate of the efficient area is high.

The present application relates to ionizable lipid molecules containing a tocopherol structure, lipid nanoparticles comprising the same and uses thereof. Specifically, an ionizable lipid molecule containing a tocopherol and derivatives thereof structure represented by formula (1), lipid nanoparticles comprising the same, methods of preparation and uses thereof are provided. Compared with ionizable lipid molecules conventionally used in the art, the lipid nanoparticles prepared from the ionizable lipid molecules represented by formula (1) of the present application can significantly improve the delivery efficiency and expression of nucleic acids.