Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method

A nucleic acid extraction reagent and coronavirus technology, which is applied in the new coronavirus nucleic acid extraction kit and extraction field, can solve the problems of time-consuming, cumbersome and complicated operations, and achieve the effect of reducing costs and reducing the false negative rate

Active Publication Date: 2022-01-14
HANGZHOU ANYU TECH CO LTD
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used nucleic acid extraction technology has a complicated extraction process, a long time, and low nucleic acid extraction efficiency, which cannot meet the needs of clinical testing; commonly used viral nucleic acid extraction and purification technologies include Trizol method, spin column method, etc.
The Trizol method separates proteins and nucleic acids by using the different distribution positions of DNA, RNA, and proteins in the organic layer and the aqueous layer. The extraction of nucleic acids is cumbersome, and the reproducibility of the extraction effect for different samples is not good. It takes at least 1.5 h, the operation is cumbersome and time-consuming, so it is not suitable for clinical testing
The operation of conventional centrifugal adsorption column method for nucleic acid extraction is relatively complicated and time-consuming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method
  • Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method
  • Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] 1. Preparation of novel biological nanomagnetic beads

[0118] In parts by weight, 1 part of FeCl 3 ·6H 2 O, 0.38 parts of FeCl 2 4H 2 O and 5 parts of 1.5M hydrochloric acid solution were added with 1.12 parts of 1M ammonia water under stirring, magnetically separated after stirring and reacting for 35min, and the resulting solid matter was washed to obtain Fe 3 o 4 ;

[0119] By weight, 0.01 part of Fe 3 o 4 Mix with 0.5 parts of distilled water, then mix with 45 parts of isopropanol, add 1 part of PEG while shaking and stirring, continue stirring for 12 minutes, add 2.5 parts of distilled water and 1 part of TEOS, magnetically separate after stirring, and wash the obtained solid substance to obtain Fe 3 o 4 @SiO 2 nano magnetic beads;

[0120] React 1 part of urea with 1 part of chloromethyltrimethoxysilane under heat reflux for 3.5 hours, conduct vacuum distillation after the reaction, and collect the target fraction to obtain the modified silane coupling...

Embodiment 2

[0129] 1. Preparation of novel biological nanomagnetic beads

[0130] With embodiment 1.

[0131] 2. Novel Coronavirus Nucleic Acid Extraction Kit

[0132] The composition and concentration of the novel coronavirus nucleic acid extraction kit are shown in Table 3 and Table 4.

[0133] Table 3 Components of the Novel Coronavirus Nucleic Acid Extraction Kit

[0134]

[0135] Table 4 Concentration of each component of the novel coronavirus nucleic acid extraction kit

[0136]

Embodiment 3

[0138] 1. Preparation of novel biological nanomagnetic beads

[0139] With embodiment 1.

[0140] 2. Novel Coronavirus Nucleic Acid Extraction Kit

[0141] The composition and concentration of the novel coronavirus nucleic acid extraction kit are shown in Table 5 and Table 6.

[0142] Table 5 Components of the Novel Coronavirus Nucleic Acid Extraction Kit

[0143]

[0144] Table 6 The concentration of each component of the new coronavirus nucleic acid extraction kit

[0145]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel coronavirus nucleic acid extraction kit and a nucleic acid extraction method, belonging to the technical field of nucleic acid extraction. The kit comprises a suspension prepared from novel biological nano-magnetic beads. A preparation method of the novel biological nano-magnetic beads comprises the step of subjecting Fe3O4@SiO2 nano-magnetic beads to reacting with a modified silane coupling agent obtained by modifying chloromethyl trimethoxysilane with urea. The method for extracting novel coronavirus nucleic acid by using the kit comprises the following steps: adding a lysis solution into a sample, carrying out pyrolysis at room temperature, adding a washing solution, carrying out oscillating and centrifuging, taking supernate, adding the novel biological nano-magnetic bead suspension, and carrying out adsorbing at room temperature; after adsorption, removing supernate, adding a washing solution, and conducting uniform mixing; performing standing, removing supernate, and carrying out airing; and after airing, adding an eluent, and carrying out eluting at room temperature to obtain an RNA solution. The kit disclosed by the invention can be used for extracting high-purity and high-concentration nucleic acid, and the extracted nucleic acid is not easy to degrade.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction, in particular to a novel coronavirus nucleic acid extraction kit and extraction method. Background technique [0002] After the virus nucleic acid is extracted and purified and identified by PCR amplification, the test results can be used as one of the clinical references. However, due to the existence of exogenous and endogenous nucleases and their relatively stable enzyme activity, it is relatively difficult to extract and purify high-quality viral nucleic acids. The formula of nucleic acid extraction reagents has a great influence on the quality and yield of nucleic acid. Therefore, the development of efficient, rapid and high yield nucleic acid extraction reagents is a subject of continuous exploration in this field. As an automated nucleic acid extraction method, the magnetic bead method is based on the specific adsorption of magnetic beads on nucleic acid in high-salt solut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30C12N15/10
CPCB01J20/06B01J20/103B01J20/22B01J20/28009B01J20/28016C12N15/1013
Inventor 冯瑞娟章贤骏陈倩倩李海锋
Owner HANGZHOU ANYU TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com