Method for constructing tissue engineering islet by adipose derived mesenchymal stem cells and obtained tissue engineering islet

Abstract

The invention discloses a method for constructing tissue engineering islet by adipose derived mesenchymal stem cells and the obtained tissue engineering islet. The method employs adipose derived mesenchymal stem cells with extensive sources to perform culture on islet acellular matrix, so that the acellular matrix can provide a three-dimensional space suitable for growth to adipose derived mesenchymal stem cells so as to promote cell adhesion, proliferation and differentiation, and formation of a three-dimensional structure engineering islet tissue able to stably secrete insulin. The method realizes gathering of insulin secreting cells, enhances cell utilization and is expected to promote the effect of repairing islet injury by tissue engineering method.

Description

technical field [0001] The invention relates to tissue-engineered islets, in particular to a method for constructing tissue-engineered islets using adipose-derived mesenchymal stem cells and the obtained tissue-engineered islets. Background technique [0002] Type I diabetes, also known as insulin-dependent diabetes mellitus, is an autoimmune disease characterized by selective destruction of islet β cells mediated by T lymphocytes, insufficient insulin secretion, and unbalanced regulation of blood sugar concentration, leading to hyperglycemia. At present, oral hypoglycemic drugs and exogenous insulin injections are the main methods for the treatment of type Ⅰ diabetes, but they cannot fundamentally cure diabetes. Islet transplantation is an effective way to completely cure diabetes, but it has been facing two key problems of insufficient donor sources and immune rejection. [0003] Adipose-derived mesenchymal stem cells are cells with multi-directional differentiation poten...

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Problems solved by technology

[0004] Acellular matrix is ​​a natural biological material. The original acellular dermal matrix was used as a dermal substitute in burn surgery, neurosurgery and other fields. With continuous development, acellular matrix has developed autologous and allogeneic and a variety of species from different sources, but there are few studies on the use of islet tissue as a raw material for the preparation of acellular matrix

At the same time, in the process of using acellular matrix to construct engineered tissues, there is a defect that the seed cells are lost in the process of replacing the seed cells with the acellular matrix in the liquid medium, which reduces the success rate of tissue construction.

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