Kit and method for detecting F-circP3F in rhabdomyosarcoma

Abstract

The invention relates to the technical field of biomedical examination, in particular to a kit for detecting fusion gene related circular RNA-F-circP3F in paraffin-embedded tissue of rhabdomyosarcoma, which comprises a reagent for detecting a molecular marker F-circP3F of rhabdomyosarcoma and a fusion gene PAX3-FOXO1, a primer composition and a probe. The nucleotide sequence of the molecular marker F-circP3F is as shown in SEQ ID No. 1 (sequence identifier number 1). The invention provides the kit and the method for detecting F-circP3F in the paraffin-embedded tissue of rhabdomyosarcoma on the basis of one-step RT-PCR and qRT-PCR technologies, absolute quantitative detection of F-circP3F mRNA in the paraffin-embedded tissue can be realized, the operability and the repeatability are high, the kit and the method can be used for batch detection, the sensitivity and the specificity are relatively high, and the kit and the method can be used for detecting F-circP3F in the paraffin-embedded tissue of rhabdomyosarcoma. And diagnosis, typing and prognosis evaluation of rhabdomyosarcoma are facilitated.

Description

technical field [0001] The invention relates to the technical field of biomedical testing, in particular to a detection kit and a detection method for detecting F-circP3F in rhabdomyosarcoma paraffin-embedded tissue. Background technique [0002] Rhabdomyosarcoma (rhabdomyosarcoma, RMS) is the most common soft tissue malignancy in children and adolescents, accounting for about 50% of all soft tissue malignancies in children. According to clinical and histomorphological characteristics, it can be divided into four types: embryonal RMS (ERMS), alveolar RMS (ARMS), spindle cell RMS (SRMS) and pleomorphic RMS (PRMS). main types. They differ not only in age of onset, location, tissue morphology, etc., but also in treatment and prognosis. [0003] So far, studies have found that there are two different types of molecular genetic changes in different subtypes of RMS: acinar RMS (ARMS) with specific chromosomal translocations t (2;13) (q35;q14), t (1; 13) (q36;q14) and its fusion...

AI Technical Summary

Problems solved by technology

Northern blot is prone to RNA degradation, although its high specificity can reduce the false positive rate, but its sensitivity is lower than that of RT-PCR, and the experimental reagents are harmful to the human body; RNAscope and BaseScope technology is a new RNA in situ hybridization technology, Although it has the characteristics of unique double Z probe design, signal amplification system and intracellular RNA quantification, it can be used for tissue in situ detection of circRNA, but the technical requirements are high and the price is expensive; circRNA sequencing (circRNA-seq) needs to construct a library, data The analysis is complicated and expensive, and the results need to be further verified by RT-PCR; the RT-PCR experimental method simplifies the experimental steps, especially the one-step RT-PCR, which has a fast reaction speed, reduces the possibility of experimental contamination, operability and repeatability Strong, can be used for batch detection, high sensitivity and specificity, is the preferred detection method for domestic and foreign scholars to study circRNA

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