Composition for inhibiting calcification of endothelial progenitor cells and detection kit

A technology of endothelial progenitor cells and a detection kit, which is applied in the field of compositions for inhibiting calcification of endothelial progenitor cells, can solve problems such as insufficient research, and achieve the effect of improving diagnosis and treatment efficiency and reducing expression

Active Publication Date: 2019-06-28
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]However, the molecular regulatory mechanism of EPCs osteogenic phenotype transformation, especially the r...

Method used

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  • Composition for inhibiting calcification of endothelial progenitor cells and detection kit
  • Composition for inhibiting calcification of endothelial progenitor cells and detection kit
  • Composition for inhibiting calcification of endothelial progenitor cells and detection kit

Examples

Experimental program
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Embodiment 1

[0028] 1. Separation and purification of EPCs derived from human peripheral blood: extract 15-20ml of peripheral blood from normal people, give lymphocyte separation medium (specific gravity 1.077) and perform density gradient centrifugation to obtain mononuclear cells, and then give CD34+ / CD133+ / KDR+ magnetic beads After sorting (Miltenyi Biotec, Bergisch Gladbach, Germany), the purity was identified by flow cytometry to be greater than 90%, and then specifically stained by Dil and FITC-UEA-1, CD34+ / CD133+ / KDR triple-positive cells combined with specific phagocytosis Dil and FITC-UEA-1 currently recognize this group of cells as EPCs, and then use this group of cells for the next experiment. Such as figure 1 Shown: figure 1 A shows the spindle shape of the EPC under the light microscope, figure 1 B shows Dil and FITC-UEA-1 specific staining, figure 1 C shows the expression levels of CD133, CD34 and KDR on the surface of EPCs identified by flow cytometry. figure 1 A and fi...

Embodiment 2

[0047] This embodiment provides a detection kit, which provides the risk of vascular calcification by detecting the gene expression of Sox9, ZBTB16, FOXO1 and Galectin-2. This prompts further examination of the patient.

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Abstract

The invention provides a composition for inhibiting calcification of endothelial progenitor cells and a detection kit. The composition for inhibiting the calcification of endothelial progenitor cellscomprises one of over-expression plasmids of Sox9, interference plasmids of ZBTB16, interference plasmids of FOXO1 and interference plasmids of Galectin-2 or a combination of at least two of the components above. The composition for inhibiting the calcification of endothelial progenitor cells can effectively reduce the formation of EPC mineralized nodules induced by an osteogenesis induction culture medium and simultaneously reduce the expression of a bone morphogenetic protein BMP-2. Furthermore, the provided detection kit can remind a user of the risk of vascular calcification, help to screen patients who need further detection, and improve the diagnosis and treatment efficiency.

Description

technical field [0001] The invention relates to a composition for inhibiting calcification of endothelial progenitor cells, and also relates to a detection kit, which belongs to the field of biotechnology. Background technique [0002] Vascular calcification, as a common way of vascular remodeling, is an independent risk factor for coronary heart disease. Vascular calcification reduces the compliance of blood vessels. PCI treatment for coronary calcified lesions often leads to difficulties in delivering balloons or stents. Insufficient expansion of stents, poor symmetry and uneven distribution are the causes of in-stent stenosis and in-stent thrombosis. one. Studies have shown that for every doubling of the coronary calcium score (CCS), the risk of coronary events increases by 15-35%. Vascular calcification is mainly divided into intimal calcification and medial calcification, and intimal calcification is more prone to plaque rupture and thrombosis, which is closely relate...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12Q1/02
Inventor 孟舒刘博裴崇哲蔺艳军
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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