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Method of detecting copy number variation of Qinchuan cattle FGF13 genes and application thereof

A technology of gene copy number and copy number variation, applied in the field of molecular genetics research, can solve the problems that have not yet been seen, and achieve the effect of accurate copy number type and low cost

Active Publication Date: 2016-05-04
NORTHWEST A & F UNIV
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Problems solved by technology

[0004] But so far, there is no report on the method of real-time quantitative PCR (quantitiveReal-TimePCR, qPCR) for the detection of Qinchuan cattle fibroblast growth factor 13 (FibroblastGrowthFactor13, FGF13) gene copy number variation (CopyNumberVariations, CNVs)

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  • Method of detecting copy number variation of Qinchuan cattle FGF13 genes and application thereof
  • Method of detecting copy number variation of Qinchuan cattle FGF13 genes and application thereof
  • Method of detecting copy number variation of Qinchuan cattle FGF13 genes and application thereof

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Embodiment Construction

[0025] The invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the invention.

[0026] In the previous research on cattle genome resequencing, it was found that the copy number variation occurred in the 621003 to 622778 positions of the bovine FGF13 genome sequence. Therefore, based on the physiological function of the FGF13 gene and the regulation mechanism of CNV, the copy number variation and growth of the FGF13 gene were studied. The correlation of traits is very necessary and can provide an important theoretical basis for molecular breeding of cattle.

[0027] The present invention designs specific primers based on the region of copy number variation in the Qinchuan cattle FGF13 genome sequence obtained by resequencing as a template, then uses Qinchuan cattle genomic DNA as a template to perform qPCR amplification, and uses the BTF3 gene as an internal reference gene, ...

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Abstract

The invention discloses a method of detecting copy number variation of Qinchuan cattle FGF13 genes and application thereof. According to the method, based on real-time quantitative PCR, Qinchuan cattle genome DNA is used as a template, a pair of specific PCR primers is utilized for amplifying a copy number variation region of the Qinchuan cattle FGF13 genes, another pair of specific PCR primers is utilized for amplifying cattle general transcription factor 3 genes as reference at the same time, and lastly a 2-delta delta Ct method is utilized for calculating and determining the individual copy number variation type. The method lays a foundation for establishment of correlation between copy number variation of the Qinchuan cattle FGF13 genes and growth traits, and is beneficial to accelerating Qinchuan cattle molecular marker assisted selective breeding work, simple, quick and convenient to apply and popularize.

Description

technical field [0001] The invention belongs to the field of molecular genetics research, and in particular relates to a method for detecting the variation of the FGF13 gene copy number of Qinchuan cattle. -ΔΔCt The value thus determines the copy number type of the individual. Background technique [0002] In the development of China's beef cattle industry, the growth rate and meat quality of beef cattle are the two main constraints. The study of the molecular mechanism regulating the growth and muscle development of beef cattle can provide a theoretical basis for the development of China's beef cattle industry. Numerous studies have shown that growth factors play a vital role in the process of regulating the growth and development of the body, and fibroblast growth factor (Fibroblast Growth Factor, FGF) plays such a role. This family of proteins is involved in a variety of physiological processes, such as embryonic development, cell growth, morphogenesis, and tissue repai...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2561/113C12Q2545/101
Inventor 陈宏蔡含芳白跃宇蓝贤勇石涛徐瑶张良志雷初朝
Owner NORTHWEST A & F UNIV
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