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Molecular marker TaSnRK2.3B related with thousand seed weight of wheat and content of soluble sugar in wheat straw and application of molecular marker TaSnRK2.3B

A thousand-grain weight and wheat technology, which is applied in the field of molecular marker TaSnRK2.3B, can solve the problems of not knowing the relationship between molecular marker agronomic traits and undeveloped TaSnRK2.3B gene.

Active Publication Date: 2017-05-31
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0003] The wheat TaSnRK2.3B gene is involved in the response to various stresses, and its overexpression can significantly improve the resistance of plants to various stresses (Tian et al.Cloning and characterization of TaSnRK2.3B, a novelSnRK2 gene in common wheat.J Exp Bot.2013,64(7):2063-2080), so it is an excellent genetic resource for stress-resistant molecular breeding of wheat. However, the molecular marker of the TaSnRK2.3B gene has not been developed so far, and there is no way to know the relationship between molecular markers and agronomic traits. relation

Method used

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  • Molecular marker TaSnRK2.3B related with thousand seed weight of wheat and content of soluble sugar in wheat straw and application of molecular marker TaSnRK2.3B
  • Molecular marker TaSnRK2.3B related with thousand seed weight of wheat and content of soluble sugar in wheat straw and application of molecular marker TaSnRK2.3B
  • Molecular marker TaSnRK2.3B related with thousand seed weight of wheat and content of soluble sugar in wheat straw and application of molecular marker TaSnRK2.3B

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Embodiment 1

[0041] Example 1. Detection of 2 SNPs and their marker polymorphisms related to 1000-grain weight, stalk soluble sugar content and plant height of wheat

[0042] 1. Specific primers for amplifying genomic DNA fragments containing two SNPs in wheat

[0043]The inventors found variation sites from 32 common hexaploid wheat varieties in Table 1 (from the National Germplasm Repository), and selected two representative SNPs to develop them as molecular markers, corresponding to the sequence table Sequence 1 from The 25th position from the 5' end (designated as the E site, there are polymorphisms of C and T), and the 295th position from the 5' end of Sequence Listing Sequence 2 (designated as the F site, there are polymorphisms of G and C) morphism); these two sites E and F mainly have two haplotypes in the natural variation population of wheat:

[0044] Monomer type A: C, G;

[0045] Monotype B: T, C.

[0046] Table 1. 32 common hexaploid wheat varieties

[0047]

[0048] ...

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Abstract

The invention discloses a molecular marker TaSnRK2.3B related with the thousand seed weight of wheat and the content of soluble sugar in wheat straw and an application of the molecular marker TaSnRK2.3B and provides a method for identification or assistant identification of to-be-detected wheat characters. The method comprises steps as follows: the genotype of the E locus and the F locus of a to-be-detected wheat population genome is detected; the to-be-detected wheat character is determined according to the genotype of the E locus and the F locus as follows: the thousand seed weight of the wheat population with the genotype of the E locus being C / C and the genotype of the F locus being G / G is higher than that of the wheat population with the genotype of the E locus being T / T and the genotype of the F locus being C / C. Experiments prove that wheat with higher thousand seed weight and smaller plant height can be found by detecting the SNP (single nucleotide polymorphism) of the two loci. The molecular marker provides a new method for molecular marker assisted selection type breeding of wheat and has great significance in culture or studies of high-yield and low-stalk wheat varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker TaSnRK2.3B related to 1000-grain weight and stalk soluble sugar content of wheat and its application. Background technique [0002] Wheat is one of the most important food crops in the world, and its production is closely related to human food security. Drought is the most important abiotic limiting factor affecting wheat production. Protein kinases are the hubs of the stress signal transmission network and play an extremely important role in the response to stress. Sucrose non-fermental protein kinase (SnRK) is a kind of serine / threonine protein kinase, of which SnRK2 is involved in the transduction of various stress signals, and plays a role in plant stress resistance, root development and crop yield formation. play a key role in. How to use SnRK2 in the breeding of new crop varieties with resistance to stress and high yield is not only the focus of molecular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 毛新国景蕊莲苗丽丽
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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